JOURNAL OF FOOD SCIENCE
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2024
·
IF 3.2
Longfei Liu,Hongling Cai,Youfeng Zhang,Qingzhe Jin,Xingguo Wang,Jun Jin
Walnut oils are of important academic and economic value, and are becoming one of the most important woody oils. Accurate and moderate refining techniques are required to produce high-quality walnut oils. In this work, walnut oils obtained from cold processing were refined in three typical techniques, mainly chemical refining, water degumming, and molecular distillation. Physicochemical properties (acid value and peroxide value [POV]), minor components (tocopherol, polyphenols, and phytosterol), oxidative stability indices, and volatile compounds were analyzed to find out the appropriate refining method for the cold-pressed walnut oils. Quality indices of all the refined oils from the three different refining methods met the requirements of the national standard, of which the POV of chemically refined oil (0.241 g/100 g) was higher than crude oil (0.058 g/100 g). Water degumming was most suitable for retaining of bioactive compounds, for example, the tocopherol was 259.40 mg/kg, the polyphenols was 44.54 mg GAE/kg, and the phytosterol was 987.32 mg/kg, but oxidation stability of the obtained oil (3.09 h) was lower than that of molecular distilled oil (4.18 h). Initial physicochemical properties especially the POV had a significant impact on oxidation stability. There is a trade-off between the retention of nutrients and extending shelf life, indicating appropriate refining techniques should be developed; that is, water degumming is suggested to be involved in producing high-quality cold-pressed walnut oils.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Meijun Liu,Yuxin Wang,Yueqi Chen,Tingting Zhang,Zhenbin Zhang,Houfen Li,Fengjun Shan,Taigang Ma
Hexavalent chromium (Cr(VI)) is one of the hazardous pollutants in wastewater because of acute toxicity and superb solubility. Graphene oxide (GO) is the promising adsorbent to handle Cr(VI), however, the drawbacks of easy aggregation, limited adsorption sites, unable valence changes and difficult recyclability hinder its further applications. In this study, Fe 3 O 4 modified L-glutamic acid functionalized graphene oxide (M−Glu/GO) was synthesized for Cr(VI) decontamination by the solvothermal method for effectively offsetting the above shortcomings. From characterization results, M−Glu/GO carried the traits of the relatively bigger surface area, abundant active sites, favorable surface charges and stable magnetic property. Cr(VI) adsorption process by M−Glu/GO was conformed to the pseudo-second order kinetic model with fast adsorption rate ( k 2 = 1.75 × 10 –3 g·mg −1 ·min −1 ) and Freundlich isotherm model with the maximum adsorption capacity of 75.63 mg·g −1 at 308 K, far more exceeding than GO (6.38 mg·g −1 ). Surprisingly, with the help of oxygen-containing groups, amine groups and Fe 3 O 4 with ample electron supplement, M−Glu/GO was capable of transforming partly Cr(VI) into Cr(III), realizing the detoxification. It exhibited practical application potentials with magnetic separation under various water matrices and kept a relative adsorption capacity of 70 % after 8 cycles. Furthermore, six interactions, involving pore filling, hydrophilic interactions, coordination interactions, anion-π interactions, electrostatic interactions and adsorption-reduction, witnessed the satisfied Cr(VI) adsorption by M−Glu/GO. This work paves a feasible way to accelerate the Cr(VI) adsorptive removal, and provides a vivid case for developing recyclable GO-functionalized adsorbents.
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Journal of Functional Foods
·
2017
·
IF 3.144
Han Peng,Wenting Li,Hongyan Li,Zeyuan Deng,Bing Zhang
Both extractable (EPE) and non-extractable bound phenolic (BPE) in the seed coat and cotyledon of black bean were investigated in the present study. Their total phenolic contents (TPC) and antioxidant activities (DPPH, ABTS and FRAP) were evaluated, and the phytochemical compositions of EPE and BPE in both seed coat and cotyledon of black soybean were identified and quantified ( Glycinemax(L.) merr ) by UPLC-ESI-QTOF-MS/MS and UPLC-ESI-QqQ-MS/MS. In the present study, a total of 112 and 78 compounds were identified or tentatively characterized in the black soybean EPE and BPE, respectively, with the majority being phenolic acids and flavonoids. Seed coat contains more abundant phenolics than that of cotyledon in all fractions. In addition, our results indicated that acid hydrolysis is more thorough than alkaline hydrolysis in the extraction of bound phenolics of black soybean. Our study provides available information for non-extractable bound phenolic constituents and their distribution in the seed coat and cotyledon of black soybean.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Shi Yong-juan,Pei Jia,Zhang Jian,Niu Jia-lin,Zhang Hua,Guo Sheng-rong,Li Zhong-hua,Yuan Guang-yin
A strategy of suppressing the fast degradation behaviour of Mg-based biomaterials by the introduction of one of Mg degradation products Mg(OH) 2 was proposed according to the following degradation mechanism, Mg + 2H 2 O ⇋ Mg(OH) 2 + H 2 ↑. Specifically, Mg(OH) 2 submicron particles were mixed into poly (L-lactic acid) (PLLA) to synthesize a composite coating onto hydrofluoric acid-pretreated Mg-Nd-Zn-Zr alloy. The in vitro degradation investigations showed that the addition of Mg(OH) 2 particles not only slowed down the corrosion of Mg matrix, but also retarded the formation of gas pockets underneath the polymer coating. Correspondingly, cytocompatibility results exhibited significant improvement of proliferation of endothelial cells, and further insights was gained into the mechanisms how the introduction of Mg(OH) 2 particles into PLLA coating affected the magnesium alloy degradation and cytocompatibility. The present study provided a promising surface modification strategy to tailor the degradation behaviour of Mg-based biomaterials.
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ENVIRONMENTAL POLLUTION
·
2016
·
IF 4.839
Xia Jia,Yonghua Zhao,Tuo Liu,Shuping Huang,Yafei Chang
Glomalin-related soil protein (GRSP), which contains glycoproteins produced by arbuscular mycorrhizal fungi (AMF), as well as non-mycorrhizal-related heat-stable proteins, lipids, and humic materials, is generally categorized into two fractions: easily extractable GRSP (EE-GRSP) and total GRSP (T-GRSP). GRSP plays an important role in soil carbon (C) sequestration and can stabilize heavy metals such as lead (Pb), cadmium (Cd), and manganese (Mn). Soil contamination by heavy metals is occurring in conjunction with rising atmospheric CO 2 in natural ecosystems due to human activities. However, the response of GRSP to elevated CO 2 combined with heavy metal contamination has not been widely reported. Here, we investigated the response of GRSP to elevated CO 2 in the rhizosphere of Robinia pseudoacacia L. seedlings in Pb- and Cd-contaminated soils. Elevated CO 2 (700 μmol mol −1 ) significantly increased T- and EE- GRSP concentrations in soils contaminated with Cd, Pb or Cd + Pb. GRSP contributed more carbon to the rhizosphere soil organic carbon pool under elevated CO 2 + heavy metals than under ambient CO 2 . The amount of Cd and Pb bound to GRSP was significantly higher under elevated (compared to ambient) CO 2 ; and elevated CO 2 increased the ratio of GRSP-bound Cd and Pb to total Cd and Pb. However, available Cd and Pb in rhizosphere soil under increased elevated CO 2 compared to ambient CO 2 . The combination of both metals and elevated CO 2 led to a significant increase in available Pb in rhizosphere soil compared to the Pb treatment alone. In conclusion, increased GRSP produced under elevated CO 2 could contribute to sequestration of soil pollutants by adsorption of Cd and Pb.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2291-2298
·
IF 4.58
Wu Huifang,Jiang Jiahui,Gu Xiaoting,Tong Changlun
Nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) with a high quantum yield (69%) and excellent photoluminescent properties were synthesized via a facile hydrothermal method using citric acid and cysteine as precursors. The cytotoxicity of N,S-CQDs was evaluated through the MTT assays using HepG2 cells as the target. The cell viability is still >95% after a 24 h incubation with particles in a concentration of up to 300 μg·mL −1 , thereby indicating low cytotoxicity and excellent biocompatibility of N,S-CQDs. The emission spectra of the N,S-CQDs are nearly independent of the excitation wavelengths in the range from 280 to 380 nm and have the strongest fluorescence emission centered at 415 nm. Co-doping with N and S promotes the electron-transfer rate and coordination interaction between N,S-CQDs and Fe(III) ions which acts as a quencher of fluorescence. Fluorescence can be recovered, however, by addition of L-Cys. Hence, the N,S-CQDs act as a highly sensitive and selective “turn-off-on” probe for the determination of Fe(III) or Cys. The limits of detection are 14 nM for Fe(III) and 0.54 μM for Cys. The interferences by 17 common metal ions and heavy metal ions, 12 other quenchers and 16 amino acids were investigated and found to be tolerable. The probe was successfully applied for cellular imaging of Fe(III) in HepG2 cells by using fluorescence microscopy.
Nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) with a high quantum yield of 69% were prepared through a facile one-step hydrothermal method. They are shown to be a highly sensitive and selective fluorescent “turn-off-on” probe for Fe(III) ions and cysteine.
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JOURNAL OF LUMINESCENCE
·
2016
·
IF 2.693
Hailun Wang,Weiju Zhu,Min Fang,Ying Xu,Cun Li
L-Glutathione (GSH) capped CdTe quantum dots (QDs) (GSH@CdTe QDs) have been successfully synthesized. GSH@CdTe QDs could selectively detect Ag + by fluorescence emission spectrometry in aqueous medium. The fluorescence intensity of GSH@CdTe QDs linearly increased upon increasing concentrations of Ag + . The detecting limit of Ag + was found to be 1.3 nM calculated from the linear concentration range of 0.02-0.2 μM. The other foreign mental ions displayed no significant response revealing good selectivity of the sensors towards Ag + . The experimental results indicated the GSH@CdTe QDs were well applied as fluorescence sensor to detect Ag + in aqueous with high sensitivity and selectivity. Furthermore, it was found that the enhancement of fluorescence intensity arose from the formation of Ag 2 Te which modified the surface traps on GSH@CdTe QDs.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Fanping Shi,Lei Wang,Yan Li,Yu Zhang,Xingguang Su
In this work, we designed a simple, convenient and high sensitive “turn-on” fluorescence platform for trypsin assay based on N -acetyl- l -cysteine capped CdTe Quantum Dots (NAC-CdTe QDs). A series of NAC-CdTe QDs with different sizes were synthesized in aqueous via refluxing routes. The fluorescence of NAC-CdTe QDs could be effectively quenched by hemoglobin (Hb) via the electron transfer interactions from NAC-CdTe QDs to hemoglobin due to the electrostatic attraction of QDs/Hb complex. In the presence of trypsin, hemoglobin would be hydrolyzed to small peptides, releasing inactive hemin molecules.Hemin has slight effect on the fluorescent intensity of NAC-CdTe QDs, so the combination of hemoglobin and NAC-CdTe QDs would be dissociated, leading to the fluorescence recovery of NAC-CdTe QDs. Therefore, we could monitor the trypsin activity by utilizing the different fluoresence responses of NAC-CdTe QDs to hemoglobin and hemin. The recovered fluorescence intensity of NAC-CdTe QDs was proportional to the logarithm of trypsin concentration in the range of 0.05–10 mU mL −1 and the detection limit for trypsin was 0.036 mU mL −1 . A model for trypsin inhibition was further established to verify the feasibility of the system. The IC 50 value is 3.06 μg mL −1 for the inhibitor from soybean. Thus, a label free real time fluorometric assay for trypsin and inhibitor screening has been developed. The established method showed a high selectivity and sensitivity for trypsin over other biological relevant enzyme, and it was applied to the determination of trypsin in human urine sample with satisfactory results.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2017
·Vol. 18
·期 6
·1246
·
IF 3.226
Nan Xiang,Xinbo Guo,Fengyuan Liu,Quan Li,Jianguang Hu,Charles Stephen Brennan
Sweet corn is one of the most widely planted crops in China. Sprouting of grains is a new processes to increase the nutritional value of grain products. The present study explores the effects of light on the nutritional quality of sweet corn sprouts. Gene expression of phenolic biosynthesis, phytochemical profiles and antioxidant activity were studied. Two treatments (light and dark) were selected and the morphological structure of sweet corn sprouts, as well as their biochemical composition were investigated to determine the effects of light on the regulation of genes responsible for nutritional compounds. Transcription analyses for three key-encoding genes in the biosynthesis of the precursors of phenolic were studied. Results revealed a negative regulation in the expression ofZmPALwith total phenolic content (TPC) in the light group. TPC and total flavonoid content (TFC) increased during germination and this was correlated with an increase in antioxidant activity (r= 0.95 and 1.0). The findings illustrate that the nutritional value of sweet corn for the consumer can be improved through germination to the euphylla stage.Keywords:germination;sweet corn;phenolic biosynthesis;antioxidant activity
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ANALYST
·
2015
·Vol. 140
·期 22
·7650-7656
·
IF 4.107
Hao-Hua Deng,Gang-Wei Wu,Dong He,Hua-Ping Peng,Ai-Lin Liu,Xing-Hua Xia,Wei Chen
Given the importance of hydrogen peroxide (H2O2) in many biological processes and its wide application in various industries, the demand for sensitive, accurate, and economical H2O2 sensors is high. In this study, we used Fenton reaction-stimulated fluorescence quenching of N-acetyl-L-cysteine-protected gold nanoclusters (NAC-AuNCs) as a reporter system for the determination of H2O2. After the experimental conditions were optimized, the sensing platform enabled the analysis of H2O2 with a limit of detection (LOD) as low as 0.027 μM. As the glucose oxidase cascade leads to the generation of H2O2 and catalase catalyzes the decomposition of H2O2, these two biocatalytic procedures can be probed by the Fenton reaction-mediated quenching of NAC-AuNCs. The LOD for glucose was found to be 0.18 μM, and the linear range was 0.39–27.22 μM. The LOD for catalase was 0.002 U mL−1, and the linear range was 0.01–0.3 U mL−1. Moreover, the proposed sensing methods were successfully applied for human serum glucose detection and the non-invasive determination of catalase activity in human saliva, demonstrating their great potential for practical applications.
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Acta Biomaterialia
·
2014
·
IF 5.684
Bin Yang,Yin Lv,Jing-yi Zhu,Yun-tao Han,Hui-zhen Jia,Wei-hai Chen,Jun Feng,Xian-zheng Zhang,Ren-xi Zhuo
The present work reports the construction of a drug delivery nanovehicle via a pH-sensitive assembly strategy for improved cellular internalization and intracellular drug liberation. Through spontaneous formation of boronate linkage in physiological conditions, phenylboronic acid-modified cholesterol was able to attach onto catechol-pending methoxypoly(ethylene glycol)- block -poly( l -lysine). This comb-type polymer can self-organize into a micellar nanoconstruction that is able to effectively encapsulate poorly water-soluble agents. The blank micelles exhibited negligible in vitro cytotoxicity, yet doxorubicin (DOX)-loaded micelles could effectively induce cell death at a level comparable to free DOX. Owing to the acid-labile feature of the boronate linkage, a reduction in environmental pH from pH 7.4 to 5.0 could trigger the dissociation of the nanoconstruction, which in turn could accelerate the liberation of entrapped drugs. Importantly, the blockage of endosomal acidification in HeLa cells by NH 4 Cl treatment significantly decreased the nuclear uptake efficiency and cell-killing effect mediated by the DOX-loaded nanoassembly, suggesting that acid-triggered destruction of the nanoconstruction is of significant importance in enhanced drug efficacy. Moreover, confocal fluorescence microscopy and flow cytometry assay revealed the effective internalization of the nanoassemblies, and their cellular uptake exhibited a cholesterol dose-dependent profile, indicating the contribution of introduced cholesterol functionality to the transmembrane process of the nanoassembly.
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CHINESE JOURNAL OF CHEMISTRY
·
2013
·Vol. 31
·期 10
·1329-1335
·
IF 0.917
Saisai Hong,Wenyu Kang,Yue Su,Yinlong Guo
In the traditional research of volatile compounds, some trace-level compounds could not be identified by gas chromatography-mass spectrometry. Target and post-targeted methods were applied in the investigation of trace-level volatile compounds in fresh turf crop ( Lolium perenne L.) based on gas chromatography in combination with hybrid quadrupole time-of-flight mass spectrometry. According to literatures published, a target analysis was performed by using retention index, accurate masses of characteristic ions and second-stage mass spectra (MS 2 spectra). And a series of experiments showed that low electron impact energy was beneficial to the improvement of the abundances of low abundance molecular ion peak. Enhancing the abundances of low abundance molecular ion peak was beneficial to qualitative analysis. Totally, 60 volatile compounds were identified, the great majority compounds of which were benzeneacetaldehyde (14.8%), 2,5-dimethyl-pyrazine (9.6%), and hexanal (9.3%). Identification was complied by mass spectral search, retention index and accurate masses of characteristic ions.
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