ELECTROCHIMICA ACTA
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2020
·
IF 6.215
Dongmiao Qin,Xiaohua Jiang,Guichun Mo,Jinsu Feng,Biyang Deng
A label-free electrochemiluminescence (ECL) immunosensor is developed to detect alpha-fetoprotein (AFP). Ru (bpy) 3 2+ -doped silica nanoparticles (Ru–SiO 2 ) and gold nanoparticle-modified reduced graphene oxide (rGO@Au) are integrated to form an rGO@Au@Ru–SiO 2 composite. The composite is employed as a carrier for immobilizing the target antibody, and a label-free ECL immunosensor is constructed via antigen–antibody interaction. Owing to the numerous Ru (bpy) 3 2+ molecules in the Ru–SiO 2 , a high ECL signal is acquired. With excellent conductivity and a large surface area, the rGO@Au composite not only accelerates electron transfer but also improved the ECL signal and captures antibodies, thus enhancing ECL response and sensitivity. Moreover, boron nitride quantum dots (BNQDs) are used as rGO@Au@Ru–SiO 2 coreactants, achieving high ECL intensity. The proposed ECL system exhibits significant potential in bioanalysis , which is attributed to the excellent biocompatibility and good stability of BNQDs. Under optimal experimental conditions, the sensor has a linear range of 0.0001 ng mL −1 to 100 ng mL −1 and a limit of detection equal to 0.03 pg mL −1 (S/N = 3). The proposed technique is successfully applied in the susceptible detection of AFP in human serum. The recovery ranges from 99% to 100%, and the RSD is less than 2.8%. The constructed ECL immunosensor exhibits acceptable performance during actual application.
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FOOD CHEMISTRY
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2020
·
IF 6.306
Manli Guo,Jingtian Chi,Chi Zhang,Minglin Wang,Hui Liang,Juying Hou,Shiyun Ai,Xiangyang Li
In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), β-galactosidase (β-Gal) and glucose oxidase (GOx) (AuNCs/β-Gal/GOx@ZIF-8) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size (<1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/β-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.
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ACS Sensors
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2020
·
IF 7.333
Xiao-Bin Huang,Shao-Hua Wu,Hao-Cheng Hu,Jian-Jun Sun
The “turn-on” mode surface-enhanced Raman scattering (SERS) aptasensor for ultrasensitive ochratoxin A (OTA) detection was developed based on the SERS “hot spots” of AuNanostar@4-MBA@Au core–shell nanostructures (AuNS@4-MBA@Au) and exonuclease III (Exo III)-assisted target cycle amplification strategy. Compared with conventional gold nanoparticles, AuNS@4-MBA@Au provides a much higher SERS enhancement factor because AuNS exhibits a larger surface roughness and the lightning rod effect, as well as an excellent electromagnetic field between the AuNS core and the Au shell, which contribute to the superstrong SERS signal. Meanwhile, Exo III-assisted target cycle amplification can be used as an effective method for the further amplified detection of OTA. Additionally, the utilization of streptavidin magnesphere paramagnetic particles offers a green, economical, and facile technology for the accumulation and separation of the signal probe AuNS@4-MBA@Au from solution. All these factors lead to a significant enhancement of detectable signals and superhigh sensitivity. As a result, the limit of detection as low as 0.25 fg mL–1 could be achieved, which was lower than that in the other reported literatures on SERS methods for OTA detection as we know. The developed SERS aptasensor also provides a promising tool for foodstuff detection.
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MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Huanhuan Li,Xiaoqian Huang,Md. Mehedi Hassan,Min Zuo,Xiangyang Wu,Yiping Chen,Quansheng Chen
Here, we proposed a novel dual-channel biosensor for rapid and sensitive detection of Hg 2+ . Compared with the previous biosensors, the [email protected] /graphene-upconversion ( [email protected] ) was prepared via a facile method and acted as a versatile signal indicator that can simultaneously readout surface-enhanced Raman spectroscopy (SERS) and fluorescence signals for Hg 2+ detection. In this strategy, the core shell magnetite colloid nanocrystal clusters-polymethacrylic acid magnetic beads (MCNCs/PMAA MBs) were synthesized and conjugated with aptamer to specific capture the Hg 2+ . The dual-channel biosensor was thus fabricated by immobilizing the [email protected] onto the surface of MBs through the complementary pairing of aptamer and complementary DNA (cDNA). Upon Hg 2+ incubation, Hg 2+ preferably bound to the MBs-aptamer, resulting in the subsequent release of preloaded [email protected] into supernatants. Under magnetic attraction, the MBs-aptamer was accumulated immediately, resulting in the concentration of Hg 2+ become homodromous proportional to the dual channel intensity of the supernatants. Under the optimized conditions, the dual channel biosensor achieved excellent performances for Hg 2+ with limits of detection (LOD) of 0.33 and 1 ppb, respectively. Furthermore, the feasibility of the biosensor to qualify Hg 2+ was also established in spiked tap water and milk samples. This strategy may provide a bridge between a highly sensitive fluorescence assay and a rapid SERS assay, it can broaden the applicability of Hg 2+ detection, which can be easy to detect Hg 2+ in real samples by coupling with SERS or fluorescence assay.
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JOURNAL OF HAZARDOUS MATERIALS
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2019
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IF 7.65
Yuanyuan Liu,Zhaoxia Cai,Long Sheng,Meihu Ma,Xiaoyun Wang
The solvated mercuric ion (Hg 2+ ) from industrial pollutants are highly toxic to the ecological environment and human health. Driven by urgent need for the selective and sensitive detection of Hg 2+ , a magnetic relaxation switching (MRS) based on Fe 3 O 4 nanoparticles (NPs) was designed. Practically, the concentrations of Hg 2+ in industrial pollutant is usually much higher than the detection range. Thus, gold nanoparticles (AuNPs) were synthesized on the surface of Fe 3 O 4 NPs to enable the visual detection of [email protected] 3 O 4 NPs. The presence of Hg 2+ in sample can specifically cause the aggregation of [email protected] 3 O 4 -aptamers NPs through T-Hg 2+ -T base pairs, leading to the change in transverse relaxation time T 2 value of detection solution. The MRS sensor showed excellent response for Hg 2+ ions in the range of 10 nM-100 nM and 100 nM to 5 μM. A highly sensitive and selective measurement of Hg 2+ was obtained with a limit of detection of 2.7 nM. Noticeably, the visual detection can qualitatively analyze the Hg 2+ beyond 5 μM by naked eye without advanced instrumentation and skilled operators.
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Frontiers of Chemical Science and Engineering
·
2020
·Vol. 14
·期 5
·813-823
·
IF 3.552
Wang Mengyun,Zhang Shengbo,Li Mei,Han Aiguo,Zhu Xinli,Ge Qingfeng,Han Jinyu,Wang Hua
Novel, hierarchical, flower-like Ag/Cu 2 O and Au/Cu 2 O nanostructures were successfully fabricated and applied as efficient electrocatalysts for the electrochemical reduction of CO 2 . Cu 2 O nanospheres with a uniform size of ∼180 nm were initially synthesized. Thereafter, Cu 2 O was used as a sacrificial template to prepare a series of Ag/Cu2O composites through galvanic replacement. By varying the Ag/Cu atomic ratio, Ag 0.125 /Cu 2 O, having a hierarchical, flower-like nanostructure with intersecting Ag nanoflakes encompassing an inner Cu 2 O sphere, was prepared. The as-prepared Ag x /Cu 2 O samples presented higher Faradaic efficiencies (FE) for CO and relatively suppressed H 2 evolution than the parent Cu 2 O nanospheres due to the combination of Ag with Cu 2 O in the former. Notably, the highest CO evolution rate was achieved with Ag 0.125 /Cu 2 O due to the larger electroactive surface area furnished by the hierarchical structure. The same hierarchical flower-like structure was also obtained for the Au 0.6 /Cu 2 O composite, where the FE CO (10%) was even higher than that of Ag 0.125 /Cu 2 O. Importantly, the results reveal that Ag 0.125 /Cu 2 O and Au 0.6 /Cu 2 O both exhibit remarkably improved stability relative to Cu 2 O. This study presents a facile method of developing hierarchical metal-oxide composites as efficient and stable electrocatalysts for the electrochemical reduction of CO 2 .
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jiahao Wu,Chao Yu,Yujie Yu,Jun Chen,Chengli Zhang,Rufei Gao,Xinyi Mu,Yanqing Geng,Junlin He
A novel dual-mode aptasensor was fabricated for ultra-sensitive detection of microcystin-LR (MC-LR). The direct method was developed using chronoamperometry for quick detection. A glass carbon electrode (GCE) was first modified with MoS 2 -PtPd nanoparticles (NPs). MoS 2 -PtPd was used as a substrate for a higher loading of aptamers and to amplify the signal by catalytic activity toward H 2 O 2 . The aptamer was then assembled onto the modified electrode surface to work as a recognition probe to bind MC-LR. To increase the sensitivity of the aptasensor, we developed an indirect method for ultra-sensitive detection through synthesizing and utilizing novel zeolitic imidazolate framework (ZIF)-8-thionine (Thi)-Au as signal material for the first time. This signal material not only possessed the high conductivity of Au NPs, but also produced current signal from a redox reaction by Thi . The complementary strand of the aptamer was immobilized on the surface of ZIF-8-Thi-Au and their compounds hybridized with the aptamer on the MoS 2 -PtPd modified GCE. MC-LR would replace ZIF-8-Thi-Au compounds by competition binding with the aptamer. The electrical signal would decrease then and be detected by SWV. Under optimal conditions, the linear ranges of the indirect and direct methods were respectively 0.01–50 and 0.1–50 ng mL −1 . And LOD of the indirect and direct method were respectively 0.006 and 0.045 ng mL −1 (signal-to-noise ratio = 3). The aptasensor ensured the accuracy of the detection outcome through comparing the results of the two modes and provided a potentially reliable and ultra-sensitive detection method of MC-LR in water environments.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Gaozhi Ou,Anshun Zhao,Hui Liao,Zhaowei Zhang,Fei Xiao
Aflatoxin in food is a significant concern that threatens human health and life and causes a heavy burden on international trade and healthcare. In this work, we developed a new type of label-free electrochemical immunosensor for the high-sensitivity determination of aflatoxin B1 (AFB1) based on Au nanoparticles decorated urchin-like Bi 2 S 3 (Au/Bi 2 S 3 ) anchored on electrochemically reduced graphene oxide (ERGO) modified carbon fiber (CF) microelectrode (Au/Bi 2 S 3 /ERGO/CF). The morphology and characterization of the modified microelectrode were investigated using scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). Under the optimum conditions, the label-free electrochemical immunosensor detected AFB1 within the linear range of 10 pg mL −1 to 20 ng mL −1 , with a low detection limit of 8 pg mL −1 and high sensitivity (0.48 μA/ng mL). The immunosensor showed high selectivity, good reproducibility, and acceptable stability. The immunosensor was applied to detecting AFB1 in cornflour samples, offering excellent reliability and accuracy compared with typical detection methods. Finally, this platform provides a superior prospect for the development of a nanohybrid immunosensor.
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Nanoscale
·
2023
·Vol. 15
·期 36
·14931-14940
·
IF 6.7
Xiang Long,Yang Yang,Zhen-Long Dou,Qu-Quan Wang,Li Zhou
Heterocrystals consisting of multiple species have received wide attention owing to the advantage of the cooperative effect contributed by different functional counterparts; therefore, a controlled growth strategy is highly desired. Herein, we report an effective method to synthesize dumbbell-like Au–PtCu solid and hollow nanorods, regulated by the unique surface capping and oxidation etching roles of copper ions. Dumbbell-like nanorods are prepared through site-selective co-deposition of platinum and copper on both tips of gold nanorods assisted by the capping effect of the CTAB–Cu+ complex to passivate the side surface. On the other hand, hollow dumbbell-like Au–PtCu nanorods are formed through triggering the etching effect of copper ions by increasing the reaction temperature to 80 °C. The manipulation of the morphology and extinction properties of the trimetallic Au–PtCu nanorods is demonstrated by adjusting the concentration of copper ions. Under excitation with a near-infrared 808 nm laser, the dumbbell-like Au–PtCu nanorods show excellent photothermal conversion, with a 3.1 times temperature increment (ΔT) compared to bare Au nanorods, while the hollow dumbbell-like Au–PtCu NRs demonstrate improved photocatalytic activity under xenon lamp irradiation.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Xiaobo Zhang,Zhiru Li,Lin Hong,Xiuwen Wang,Jijuan Cao
Fumonisin B1 (FB1), as one of the highest toxicity mycotoxins, poses a serious threat to animal and human health, even at low concentrations. It is significant and challenging to develop a sensitive and reliable analytical device. Herein, a paper-based electrochemical aptasensor was designed utilizing tetrahedral DNA nanostructures (TDNs) to controllably anchor an aptamer (Apt), improving the recognition efficiency of Apt to its target. First, gold nanoparticles (AuNPs)@MXenes were used as a sensing substrate with good conductivity and modified on the electrode for immobilization of complementary DNA–TDNs (cDNA–TDNs). In the absence of FB1, numerous Apt–Au@Pt nanocrystals (NCs) was hybridized with cDNA and assembled on the sensing interface, which accelerated the oxidation of TMB with H2O2 and produced a highly amplified differential pulse voltammetry (DPV) signal. When the target FB1 specifically bound to its Apt, the electrochemical signal was decreased by releasing the Apt–Au@Pt NCs from double-stranded DNA (dsDNA). On account of the strand displacement reaction by FB1 triggering, the aptasensor had a wider dynamic linear range (from 50 fg/mL to 100 ng/mL) with a lower limit of detection (21 fg/mL) under the optimized conditions. More impressively, the designed FB1 aptasensor exhibited satisfactory performance in corn and wheat samples. Therefore, the TDN-engineered sensing platform opens an effective approach for sensitive and accurate analysis of FB1, holding strong potential in food safety and public health.
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RARE METALS
·
2023
·
IF 8.8
Zhuang Wen-Chang,Zhang Hai-Yue,Chen Zhen-Yang,Cheng Wen-Jing,Na Wei-Dan,Li Zhao,Tian Lin
Paper-based sensing platform is a point of need analytical toolkit for safety testing. However, the sensitivity, specificity, and simplicity are still challenging. Herein, we report a novel strategy (Au/δ-MnO 2 hollow nanosphere and 3,3′,5,5′-tetramethylbenzidine (TMB) induced test strips for signal-on detection) that can be utilized for hexavalent chromium (Cr 6+ ) detection. Interestingly, Cr 6+ (CrO 4 2− ) as a smart switch can remarkably enhance the oxidase-like activity of Au/δ-MnO 2 hollow nanosphere. The presence of Cr 6+ can regulate the surface electronic redistribution of Au/δ-MnO 2 and adjust the geometric configuration, which leads to the improvement in oxidase-like activity of Au/δ-MnO 2 . As a proof-of-concept application, a visual paper-based sensing platform of Cr 6+ along with quantitative analysis by the test strips was successfully constructed. This paper-based sensing platform exhibits a linear range with excellent selectivity for other interfering substances and lower limit of detection of 0.09 μmol·L −1 , providing a promising toolkit at-home Cr 6+ measurement and environmental monitoring. Graphical abstract
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Peifang Chen,Caiyun Jiang,Zhouping Wang,Hong-zhen Lian,Xiaoyuan Ma
As a virulent metabolite, aflatoxin B 1 (AFB 1 ) presented in various cereal grain is tightly implicated in severe human diseases. In this study, 3D plasmonic nanohybirds of Raman molecule 4-mercaptobenzoic acid (4-MBA)-embedded and AFB 1 aptamer-modified bimetallic nanostars as SERS probes bound to magnetic nanoflowers were fabricated and demonstrated as a high-performance SERS-active aptasensor to quantitatively analyze AFB 1 . Bimetallic Au@Ag SERS plasmonic nanoprobes with enhanced properties were capable of enhancing discriminative Raman peaks of 4-MBA. Then, the integration of iron tetroxide nanoparticles (Fe 3 O 4 NPs) and molybdenum disulfide nanosheets (MoS 2 NShs) with huge specific surface area constituted stable 3D Fe 3 O 4 @MoS 2 plasmonic nanoflowers, facilitating the bind of numerous aptamer-based SERS probes via the non-covalent interaction between MoS 2 NShs and aptamer, which were ideal candidates for SERS-active substrates. Additionally, Fe 3 O 4 NPs as magnetic core endowed 3D nanocomposites with specific magnetic separation characteristic that caused the collected SERS hotspots to exhibit superior signal response, and further strengthening the sensitivity in a complex food matrix. Aptamer-target AFB 1 specific recognition triggered linearly diminished 4-MBA signal intensity (I 4-MBA ) on the substrate to achieve a low detection limit of 58.9 pg/mL. Furthermore, the sensor has the potential to be a promising monitoring tool for other trace contaminants.
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