ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 409
·期 26
·6081-6090
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IF 3.431
Sheng Zhen,Chen Ligang
Abstract The concentration of L-cysteine (Cys) and glutathione (GSH) is closely related to the critical risk of various diseases. In our study, a new rapid method for the determination of Cys and GSH in water and urine samples has been developed using a fluorescent probe technique, which was based on crystal violet (CV)-functionalized CdTe quantum dots (QDs). The original QDs emitted fluorescence light, which was turned off upon adding CV. This conjugation of CV and QDs could be attributed to electrostatic interaction between COO − of mercaptopropionic acid (MPA) on the surface of QDs and N + of CV in aqueous solution. In addition, Förster resonance energy transfer (FRET) also occurred between CdTe QDs and CV. After adding Cys or GSH to the solution, Cys or GSH exhibited a stronger binding preference toward Cd 2+ than Cd 2+ -MPA, which disturbed the interaction between MPA and QDs. Thus, most MPA was able to be separated from the surface of QDs because of the participation of Cys or GSH. Then, the fluorescence intensity of the CdTe QDs was enhanced. Good linear relationships were obtained in the range of 0.02–40 μg mL −1 and 0.02–50 μg mL −1 , and the detection limits were calculated as 10.5 ng mL −1 and 8.2 ng mL −1 , for Cys and GSH, respectively. In addition, the concentrations of biological thiols in water and urine samples were determined by the standard addition method using Cys as the standard; the quantitative recoveries were in the range of 97.3–105.8%, and relative standard deviations (RSDs) ranged from 2.5 to 3.7%. The method had several unique properties, such as simplicity, lower cost, high sensitivity, and environmental acceptability.
Crystal violet-functionalized CdTe quantum dots for detecting L-cysteine and glutathione with switch-on fluorescent strategy
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高影响因子
Biomaterials Science
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2024
·
IF 5.8
Shiya Zhuang,Xingwei Jin,Lian Cen,Yuan Shao
Intravesical instillation is an effective post-treatment for bladder cancer performed by delivering medications directly into the bladder to target the remaining cancer cells. The current study thus aimed to develop porous poly(L-lactide-co-ε-caprolactone) (PLCL) microspheres encapsulated with 10-hydroxycamptothecin (HCPT) via microfluidics to serve as a drug delivery system with persistent floating capacity and sustained HCPT-release property for intravesical instillation. A microfluidic device was designed to fabricate PLCL microspheres and encapsulate HCPT (HCPT-MS) within them; methanol and tridecane were introduced into an oil phase as a co-solvent and pore-forming agent, respectively, to regulate the floating ability of microspheres. The physicochemical properties of the resulting microspheres were characterized, and the floating behavior, release profile and anti-tumor effects of HCPT-MS were investigated. The obtained spherical HCPT-MS were 119.23 μm in size, monodisperse, and featured a porous concave surface and hollow structure. The encapsulation efficiency and drug loading of HCPT within HCPT-MS was around 67% and 4.9%, respectively. HCPT-MS exhibited impressive floating capabilities in water, PBS and artificial urine even in a simulated bladder dynamic environment. These microspheres remained afloat after being subjected to 90 repeated simulated urination processes. The sustained release of HCPT from these floating microspheres lasted for more than 10 days. The IC50 (half maximal inhibitory concentration) of HCPT-MS was calculated to be 52.14 μg mL−1. T24 cells (human bladder cancer cells) when cultured with HCPT-MS at such a concentration were severely inhibited, and the inhibition further enhanced with an increase in culture time. Hence, the feasibility of the current porous and floating HCPT-MS as a formulation for intravesical instillation to deliver medications into the bladder with sustained release and stability was thus substantiated.
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Food Bioscience
·
2025
·
IF 5.9
Mengli Wang,Xinjing Li,Foxin Zhang,Heping Cui,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
Extra-added l- cysteine effectively inhibited the browning of Maillard peptides (MPs) derived from Lentinus edodes . Meanwhile, an unsaturated fatty acid (UFA) was introduced to increase the content of 1-octen-3-ol (mushroom alcohol) to enhance the mushroom aroma of the MPs. Among the three UFAs tested, linoleic acid (LA) exhibited the strongest promoting effect on 1-octen-3-ol, resulting in a 1-octen-3-ol content of 74.57 ± 1.48 μg/L in MPs. When LA was co-heated with l- cysteine and/or xylose, a synergistic effect was observed between LA and l- cysteine, leading to the highest 1-octen-3-ol content of 211.29 ± 3.62 μg/L in LA- l- cysteine-xylose system. The enzymatic hydrolysate of commercial safflower seed oil was further used as an alternative source of LA to fortify the typical mushroom flavor. The incorporation of 25 % safflower seed oil hydrolysate was recommended to enhance the 1-octen-3-ol production (133.09 ± 8.74 μg/L) in MPs. These findings provide new ideas for enhancing the mushroom aroma, offering potential applications in flavor design and food processing.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Na Li,Yefan Wang,Zhifeng Tan,Yunpeng Xu,Xiaoyang Liu,Yuxin Liu,Dayong Zhou,Deyang Li
This study used ultra-high pressure processing (HPP) heat-assisted technology combined with L-cysteine (L-cys) to process ready-to-eat (RTE) shrimp. Subsequently, the effects of physical field and chemical modifications on the color of RTE shrimp were studied. The results showed that the RTE shrimp treated with HPP-Heat-L-cys showed better performance in terms of brightness value (65.25) and astaxanthin (AST) content (0.71 μg/g) during storage, maintaining the original color of RTE shrimp effectively. In addition, it was observed that the application of HPP-Heat-L-cys significantly delayed phenol oxidation, lipid oxidation, and Maillard reaction compared with traditional HPP or heat treatments. Specifically, the total phenolic content of RTE shrimp treated with HPP-Heat-L-cys was higher than that of other samples, but the TBARS and browning index were lower. Furthermore, HPP-Heat-L-cys could delay the production of dark products (such as 2-methylanthraquinone, p-benzoquinone, lipofuscin and melanin), ultimately safeguarding the color stability of RTE shrimp during storage.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Shiyang Li,Chenchen Zheng,Longdou Tu,Dajian Cai,Yangxiang Huang,Congjie Gao,Yeqiang Lu,Lixin Xue
Tight ultra-filtration (TUF) membranes were constructed by in situ growing zinc imidazole frameworks micro-crystalline leaves (ZIF-L) in polyethylene imine (PEI) and polydopamine (PDA) deposit layers on porous polyethylene (PE) substrates. The effects of preparation conditions on the surface physical and chemical structures as well as on the dye/salt separation performance of the formed TUF membranes were systematically investigated. By inserting selective water permeation channels and increasing contacting surface areas, in situ-grown ZIF-L arrays tightly cross-linked in the coating matrix greatly increased water permeation without trading off dye/salt retention selectivity. The morphology of the included ZIF-L particles could be varied by adjusting the ligand/Zn molar ratio (α) in the preparation processes. Optimized PDA-PEI/ZIF-L@PE TUF membranes containing ZIF-L of cross-cross block morphology showed very high pure water permeability of 180 ± 20 L·m −2 ·h −1 ·bar −1 (LMHB) and retention selectivity (S CR/Na2SO4 and S MB/Na2SO4 ) of 267 and 43, respectively, as well as excellent stability and anti-fouling properties.
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JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 3.6
Jiatao Zhang,Tungalag Dong,Yuan Zhang,Jia Guo,Yulu Li,Xueyan Yun
Although poly(L-lactic acid) (PLLA) is biocompatible and degradable, its inherent brittleness, susceptibility to aging, low ultraviolet light (UV) resistance, and moderate gas barrier properties limit its diverse applications. In this study, graphene oxide (GO) was used as an initiator, unsaturated poly (butylene itaconate) (PBI) was used as a flexible chain segment, and GO-grafted P(LA- g -BI) (GO- g -PLBI) copolymer was synthesized by in situ melt polycondensation of lactic acid. Nuclear magnetic resonance, Fourier transform infrared, and X-ray photoelectron spectroscopy results confirmed the successful synthesis of GO-g-PLBI and the formation of a multi-branched structure. The GO- g -PLBI film demonstrated excellent ductility and oxygen barrier properties, with elongation at break increased by 73.8 times compared with PLLA, while oxygen permeability (OP) decreased by 40.3%. The incorporation of PBI facilitated micro-crosslinking within the copolymer, thereby enhancing its thermal stability. More gg and gt conformations were formed in the copolymer, while the crystallization of the copolymer induced by aging was inhibited, thus showing excellent anti-aging properties. Interestingly, the OP value of this micro-crosslinked GO-g-PLBI film decreased by 60.9% after physical aging, demonstrating extremely high oxygen barrier properties. This study offers a viable approach to developing PLLA/graphene nanocomposites that exhibit adjustable flexibility, enhanced oxygen barrier, heat and UV resistance, anti-aging properties, and high transparency. Graphical
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Mengting Lai,Hongzhu Chen,Xiaozhen Liu,Fuxiang Li,Fengyuan Liu,Yuting Li,Jingkun Yan,Li Lin
The aim of this study was to reveal the phenolic profile of mangosteen ( Garcinia mangostana L. ) pericarp and investigate their inhibitory effects on α-amylase and α-glucosidase. Mangosteen pericarp polyphenols (MPP) obtained by acetone extraction were fractionated with gradient ethanol elution. A total of twenty nine phenolic chemicals were found and quantified by UPLC-Q-TOF-MS/MS. MPP eluted by the 60% ethanol solution (MPP 60% ) showed the highest total phenolic content. Moreover, MPP 60% displayed the greatest inhibition activities on α-amylase and α-glycosidase by competitive and mixed inhibitions. Mechanistically, the inhibitory effects of MPP 60% on α-amylase and α-glucosidase involved the changes of the secondary structure as well as the static fluorescence quenching of these enzymes. Pearson correlation coefficients revealed that proanthocyanidin B2, quercetin glucoside, and mangostain contributed most to inhibiting the activities of α-amylase and α-glucosidase. This study indicated that polyphenols from mangosteen pericarps can be developed into potential inhibitors of α-amylase and α-glucosidase, offering a viable strategy for the valorization of mangosteen pericarps.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zhe Lin,Zhengcai Guo,Xiaohong Wang,Jinchun Tu,Delun Chen,Yang Cao,Qiang Wu
MoS 2 is an attractive electrochemical sensing electrode material for the detection of biomolecule because of its adjustable band gap, large surface area, and unique electrochemical characteristics. However, the poor catalytic and electrical conductivity of the semiconductor 2H-phase MoS 2 and the phenomenon of restacking during electrochemical use hinder its sensing application. Herein, electron injection engineering is employed for the formation and stabilization of high-concentration metal 1T-phase MoS 2 nanosheets on ionic liquid-functionalized carbon nanotubes (CNTs-IL). The increase of MoS 2 concentration in the metal 1T phase significantly accelerates electron transfer kinetics. The in-situ growth method results in a tightly interfaced coupling effect, thereby substantially shortening the electron transport pathway and decreasing interfacial transmission impedance. In addition, the sulfur defects on the surface of MoS 2 as the anchoring point of sulfhydryl groups promote the enrichment of thiols on the surface of the material and reduce the reaction barrier for sulfhydryl oxidation, thereby improving the catalytic performance. The prepared CNTs-IL-MoS 2 /SPE biosensor shows excellent l -cysteine sensing performance by electrochemical detection.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Qianyi Wei,Yongrong Zhou,Zhengtao Hu,Ye Shi,Qing Ning,Keyun Ren,Xinyu Guo,Ronglin Zhong,Zhi Xia,Yinghao Yin,Yongxin Hu,Yingjie Wei,Ziqi Shi
Ethnopharmacological relevance Psoraleae Fructus (Bu Gu Zhi) is the fruit of Psoralea corylifolia L. (PCL) and has been used for centuries in traditional Chinese medicine formulas to treat osteoporosis (OP). A new drug called "BX" has been developed from PCL, but its mechanism for treating OP is not yet fully understood. Aim of the study To explore the mechanism of action of BX in the treatment of ovariectomy-induced OP based function-oriented multi-omics analysis of gut microbiota (GM) and metabolites. Materials and methods C57BL/6 mice were bilaterally ovariectomized to replicate the OP model. The therapeutic efficacy of BX was evaluated by bone parameters (BMD, BV/TV, Tb.N, Tb.Sp), hematoxylin and eosin (H&E) staining results, and determination of bone formation markers procollagen type Ⅰ amino-terminal peptide (PⅠNP) and bone-specific alkaline phosphatase (BALP). Serum and fecal metabolomics and high-throughput 16S rDNA sequencing were performed to evaluate effects on endogenous metabolites and GM. In addition, an enzyme-based functional correlation algorithm (EBFC) algorithm was used to investigate functional correlations between GM and metabolites. Results BX improved OP in OVX mice by increasing BMD, BV/TV, serum PⅠNP, BALP, and improving Tb.N and Tb.Sp. A total of 59 differential metabolites were identified, and 9 metabolic pathways, including arachidonic acid metabolism, glycerophospholipid metabolism, purine metabolism, and tryptophan metabolism, were found to be involved in the progression of OP. EBFC analysis results revealed that the enzymes related to purine and tryptophan metabolism, which are from Lachnospiraceae_NK4A136_group , Blautia , Rs-E47_termite_group , UCG-009, and Clostridia_UCG-014 , were identified as the intrinsic link between GM and metabolites. Conclusions The regulation of GM and restoration of metabolic disorders may be the mechanisms of action of BX in alleviating OP. This research provides insights into the function-oriented mechanism discovery of traditional Chinese medicine in the treatment of OP.
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高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2024
·
IF 5.9
Yi Guo,Jinchao Chen,Chunmei Gao,Shifeng Ji,Yunqing Xing,Wenjing Tan
In this paper, ZIF-L was blended with citric acid (CA) and polyethersulfone (PES) to prepare a casting solution. A novel negatively charged ultrafiltration (UF) membrane (ZIF-L/CA/PES) was obtained using the reverse thermally induced phase separation (RTIPS) method. The hydrophilic carboxyl groups present in CA not only imparted high hydrophilicity and negative charge to the membrane surface, enhancing the membrane’s selectivity and contamination resistance, but also created an acidic environment to attack the Zn atoms in ZIF-L by the ionized H + from CA, resulting in the generation of micro-defective pores and an improvement in permeability. Additionally, the results showed that membranes generated through the RTIPS process exhibit uniform porous surfaces and spongy cross-sections, enhancing membrane performance. The optimized membranes demonstrated the capability to achieve a pure water flux of up to 2267 L/m 2 h with 93.1 % humic acid (HA) retention, and a remarkable membrane flux recovery rate of 75.8 %, suggesting the crucial roles played by CA and ZIF-L in the separation process. In conclusion, hydrophilic anti-fouling UF membranes with a high negative charge and excellent permeation selectivity were fabricated by establishing micro-defective channels through the incorporation of carboxylic acid and ZIF-L, combined with the RTIPS process.
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高影响因子
CEREAL CHEMISTRY
·
2024
·Vol. 101
·期 4
·884-897
·
IF 2.2
Zhaogui Chen,Chunmei Chen,Cheng Chen,Siyu Chen,Weixi Li,Zhengbiao Gu,Yan Hong
Backgrounds and Objectives Fermented rice cake is a common traditional fermented food in China with a crystal clear color, unique flavor, dense honeycomb structure, and soft and delicate texture. As its raw materials are not uniform, different batches often have poor product quality stability and different volatile flavor components. Therefore, this study investigates the effects of different indica rice flours on processing adaptability and their impacts on the flavor characteristics. Findings The results show that the seven indica rice flours have significantly different basic compositions and physicochemical properties ( p < .05). Specifically, the fermented rice cake prepared with TG2 showed a large specific volume, good elasticity, bright color, high volatile flavor compound contents, and the maximum comprehensive sensory evaluation score. Additionally, partial least squares discriminant analysis was conducted, and three volatile compounds were identified with variable importance for the projection >1 and odor activity value >1 as the criteria, namely, ethanol, styrene, and phenethyl alcohol, which played major roles in forming the overall aroma of fermented rice cakes. Conclusions The texture, color, and sensory score of fermented rice cakes are closely related to the basic components of the indica rice flour, and the most popular fermented rice cakes have a specific volume of no less than 2 mL/g, an L* of above 78, and a TPA-tested elasticity of about 3 mm. Significance and Novelty This study may help improve the processing of fermented rice cakes and provide a theoretical basis for their production and flavor control.
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Food Bioscience
·
2024
·
IF 4.8
Yujie Chen,Hehua Lei,Zheng Cao,Cui Zhang,Lijun Liu,Xin Gao,Quanzhi Qin,Limin Zhang,Gang Chen
Sacha inchi (SI, Plukenetia volubilis L.) is a South American oleaginous crop with multiple health benefits and has been traditionally used to treat various ailments due to its bioactive ingredients such as polyphenols and flavonoids. In this study, a variety of compounds including three flavonoids (epigallocatechin, luteolin-3′,7-di-O-glucoside and cosmosiin), two polyphenols (gallic acid and 4-hydroxycoumarin) and three organic acids (malic acid, citric acid and 2-isopropylmalic acid) were firstly identified by UHPLC-QQQ-MS. Subsequently, in vitro results show that SI leaf aqueous extract (SILE) exhibit potential to lower uric acid (UA) by affecting mRNA levels of UA synthases (XO and HGPRT) with neglectable impacts on cell viability of liver cells (L02). In vivo experiments further suggest that SILE can effectively reduce the levels of serum UA and renal inflammatory cytokines ( Il-1β , Il-6 and Tnf-α ), and improve renal function indicators (serum Cr and BUN), thereby repairing kidney injury in both acute and chronic hyperuricemic (HUA) animal models. In addition, SILE also improve UA metabolism by inhibiting UA biosynthesis and reabsorption and promoting UA excretion. 1 H NMR-based metabolomics reveals that SILE effectively regulate the levels of glucose, amino acid, purine and pyrimidine that are involved in UA metabolic pathway in kidney of HUA mice. These findings demonstrate that SILE rich in polyphenols and flavonoids has a great potential for HUA treatment by inhibiting xanthine oxidase activity and improving UA metabolism.
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