Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Zhang Tao,Zhu Xiaoqing,Xiong Jiyang,Xue Zhixin,Cao Yunteng,Gordon Keith C.,Xu Guiyin,Zhu Meifang
The electrostatic effects of separators under the internal electric field are often overlooked, leading to the unreliability of traditional theoretical models. Here we introduce the dielectric constant as a descriptor and develop a high dielectric constant fiber separator primarily composed of phosphorylated cellulose. Under the internal electric field, the intense electron displacement polarization within the high dielectric constant separator enhances the charge transfer kinetics and optimizes the solvation structure, thus mitigating the formation of amorphous organic oligomers at the solid-electrolyte interphase. Furthermore, the separator induces the formation of LiF, thereby forming a robust and low-resistance solid-electrolyte interphase. The separator exhibits high ionic conductivity (0.76 mS cm −1 at 25 °C) and Li + transference number (0.68). Consequently, the Li||LiFePO 4 pouch cell with the prepared separator achieve high specific energy exceeding 350 Wh kg −1 (relative to the mass of pouch cells) under practical quantities of active materials and electrolyte.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 10
·1327-1337
·
IF 2.7
Yi Jing,Zhang Maifei,Zhu Lin,Xu Changzhi,Li Binglin,Wu Panpan,Wu Hang,Zhang Buchang
Purpose Viruses, such as Ebola virus (EBOV), evolve rapidly and threaten the human health. There is a great demand to exploit efficient gene-editing techniques for the identification of virus to probe virulence mechanism for drug development. Methods Based on lambda Red recombination in Escherichia coli ( E. coli ), counter-selection, and in vitro annealing, a high-efficiency genetic method was utilized here for precisely engineering viruses. EBOV trVLPs assay and dual luciferase reporter assay were used to further test the effect of mutations on virus replication. Results Considering the significance of matrix protein VP24 in EBOV replication, the types of mutations within vp24 , including several single-base substitutions, one double-base substitution, two seamless deletions, and one targeted insertion, were generated on the multi-copy plasmid of E. coli . Further, the length of the homology arms for recombination and in vitro annealing, and the amount of DNA cassettes and linear plasmids were optimized to create a more elaborate and cost-efficient protocol than original approach. The effects of VP24 mutations on the expression of a reporter gene (luciferase) from the EBOV minigenome were determined, and results indicated that mutations of key sites within VP24 have significant impacts on EBOV replication. Conclusion This precise mutagenesis method will facilitate effective and simple editing of viral genes in E. coli .
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 11
·2687-2693
·
IF 3.578
Huang Li-Li,Jin Yong-Jie,Zhao Dongxu,Yu Chao,Hao Jian,Xie Hai-Yan
The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.
Initially, the 3-amino-phenylboronic acid (APBA) reacted with QDs-COOH to prepare the PBA capped QDs (QDs-PBA). Then, the vesicular stomatitis virus (VSV), on which the surface of the envelope is abundant with sialic acid (SA), could be labeled efficiently and specifically with QDs-PBA in one step based on sialic acid-phenylboronic acid recognition system. The labeling efficiency attained to (83 ± 4.3 %) (mean ± SD). The labeled VSA (QDs-VSV) was still infectious and capable of being imaged at the single virion level
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-7
·
IF 5.479
Zhang Limei,Qin Ju,Yang Qiang,Wei Siqi,Yang Rui
Carbon dots (CDs) were hydrothermally prepared from gelatin as the sole precursor. The CDs were hybridized with CoOOH nanosheets (NSs) to obtain a fluorescent probe (CD-CoOOH) for ascorbic acid (AA). The blue luminescence of the CDs, with excitation/emission maxima of 350/443 nm, is quenched by the NSs. If the NSs are reduced to Co 2+ by addition of AA, the CD-CoOOH hybrid is disintegrated and fluorescence recovers. The hybrid nanoprobe has a linear response in the 0.8–12.5 μM and 12.5–690 μM AA concentration ranges and a 0.025 μM limit of detection. Compared to previously reported methods for detecting AA, this methods has a wider linear range and a lower detection limit. The nanoprobe was utilized to quantify AA in spiked human serum samples and gave satisfactory results. The strategy was also applied to the construction of an “AND” logic gate, which avoided perplexed material decorations and chemical tracking.
Scheme 1. Schematic illustrations of the preparation of CDs and of the working principle for determination of ascorbic acid (AA).
✓ 已验证
DOI
PubMed
高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 17
·9089-9104
·
IF 6.123
Zhao Yang,Jin Bo,Zheng Tian,Peng Rufang
A novel natural macromolecule stabilizer, tea polyphenol, was developed based on the scavenging effect of nitroxide radical to enhance the thermal stability of nitrocellulose. The structural of tea polyphenols was confirmed by infrared spectroscopy, and their good thermal stability of tea polyphenols in nitrocellulose was confirmed by methyl violet paper test and vacuum stability test. The iso-thermogravimetric test showed that tea polyphenols could prolong the safe storage life of nitrocellulose. The inhibition of tea polyphenols on the autocatalytic thermal decomposition of nitrocellulose in the early stage of nitrocellulose thermal decomposition was confirmed by SEM images at different stages of nitrocellulose thermal decomposition. The thermal interaction between tea polyphenols and nitrocellulose was further analyzed by non-isothermal thermal analysis. Experiments confirmed that tea polyphenols can enhance the thermal stability of nitrocellulose. Their addition increased the T 0 of nitrocellulose by 2.0–9.4 ℃, the T bp 0 by 1.4–9.6 ℃, and the activation energy of nitrocellulose by 5.38–9.77 kJ·mol –1 .
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-12
·
IF 3.998
Wu Yifan,Li Lingtong,Chen Shaopeng,Qin Jun,Chen Xiaolang,Zhou Dengfeng,Wu Hong
Poly( D -lactic acid) (PDLA) with different polyethylene glycol (PEG) segment synthesized PDLA-PEG-PDLA triblock copolymer through the ring-opening reaction of D -LA and PEG will be used as a toughening modifier. The microstructure, crystal structures and crystallization behaviors of this triblock copolymer were investigated by Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC) and polarized optical microscopy (POM). The triblock copolymer is synthesized from the appearance of CH 2 stretching vibration peak at 2910 cm −1 and C-O stretching vibration peak at 1200 cm −1 from PEG in FTIR spectra. Moreover, the chemical shift that is about 3.6 ppm in 1 H NMR and 68.8ppm in 13 C NMR proves this matter. The results of XRD and DSC reveal that PDLA and PEG are crystallized separately, and are not fully compatible, and microphase separation has occurred in this triblock copolymer. PEG can induce the triblock copolymer to accelerate the rate of crystallization, allowing it to crystallize more completely in the same amount of time. When the molecular weight of PEG is 6000 or the ratio of D -LA/PEG is 1/1, the crystallizability of PDLA-PEG-PDLA triblock copolymer is the best.
✓ 已验证
DOI
PubMed
高影响因子
Science Bulletin
·
2015
·Vol. 60
·期 12
·1122-1127
·
IF 0
Liu Shuang,Wu Jia-Ke,Fan Cong-Cheng,Xue Guo-Biao,Chen Hong-Zheng,Xin Huolin L.,Li Han-Ying
A simple solution processing method was developed to grow large-scale well-aligned single crystals including 6,13-bis(triisopropylsilylethynyl)pentacene (TIPS-pentacene), anthracene, tetracene, perylene, C 60 and tetracyanoquinodimethane. As pinned by a solid needle, a droplet of semiconductor solution dried into single-crystal arrays on a 1 cm × 2 cm substrate. TIPS-pentacene was used to demonstrate the fabrication of hundreds of field-effect transistors (FETs) with the hole mobility as high as 6.46 cm 2 V −1 s −1 . As such, this work provides a high-throughput, yet efficient approach for statistical examination on the FET performance of organic single crystals.
✓ 已验证
DOI
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2017
·Vol. 244
·期 5
·851-860
·
IF 1.664
Xu Dan,Liu Hong,Jin Cheng-yu,Cao Chun-mei,Li Wen-gang,Zeng Fan-kui,Zhao Yu-ci,Liu Gang
Hongmei is a new potato variety developed in recent year, which is suitable for raw eating as a kind of fruit. In this study, sensory evaluations, nutritional components, morphology characteristics of starch granules, phenolic compounds, sugars, glycoalkaloids, and amino acid composition of potato Hongmei were investigated. The results show that Hongmei be suitable for raw eating mainly based on three reasons: (1) possess a juicy characteristic and lower earthy smell, with a distinctive fruity flavor of fresh cucumber; (2) the average particle size of starch from Hongmei is 18.31 ± 7.71 μm, which is smaller than the commercial potato starch significant ( p < 0.01); (3) with a content of 653.64 ± 83.25 mg/100 g of fructose, which is similar to the sweet potato be suitable for raw eating. The results also show that Hongmei had high concentrations of crude protein, total ascorbic acid, but lower starch content. The total glycoalkaloids content in the flesh is only 5.52 mg/kg, which implied that it is safe for raw eating. However, due to the total glycoalkaloids in the Hongmei peel is 358.88 mg/kg, which is higher than the safe standard of 200 mg/kg, it is recommended to peel when eating raw.
✓ 已验证
DOI
高影响因子
IONICS
·
2020
·Vol. 26
·期 4
·1787-1795
·
IF 2.394
Ge Zhi,Li Jie,Liu Jin
A flexible PEO-NaCF 3 SO 3 -MIL-53(Al) solid electrolyte is fabricated for all-solid-state sodium-sulfur batteries (ASSBs). When the mole ratio of EO (ethylene oxide of PEO):Na (sodium ion of NaCF 3 SO 3 ) is 20 and MIL-53(Al) is 3.24 wt%, high ionic conductivities of 6.87 × 10 −5 S cm −1 at 60 °C and 6.52 × 10 −4 S cm −1 at 100 °C are achieved. And the sodium ion transference number is significantly increased to 0.40 from 0.13 compared to the PEO-NaCF 3 SO 3 solid electrolyte without MIL-53(Al). When it is assembled to ASSBs, the batteries obtain high capacity retention and reversible current-rate capability. At 0.1 C and 60 °C, the solid-state battery delivers the first discharge capacity of 897.7 mAh g −1 and 674.9 mAh g −1 after 50 cycles with a coulombic efficiency near 100%. The enhanced electrochemical performances of the solid electrolyte, as well as ASSBs, are benefited from MIL-53(Al) filler.
✓ 已验证
DOI
高影响因子
Advanced Fiber Materials
·
2020
·Vol. 2
·期 2
·118-122
·
IF 0
Sun Yijun,Mwandeje Jackson Beja,Wangatia Lodrick M.,Zabihi Fatemeh,Nedeljković Jovan,Yang Shengyuan
The improvement of the photocatalytic performance of TiO 2 nanofibers (NFs), prepared by electrospinning, is achieved by surface modification with the rhodizonic acid (RhA). The condensation reaction between hydroxyl groups from TiO 2 NFs and RhA is accompanied by the red-shift of optical absorption due to interfacial charge transfer (ICT) complex formation. Crystal structure, morphology, and optical properties of unmodified and surface-modified TiO 2 NFs were analyzed. The photocatalytic performance of prepared samples has been examined through degradation of organic dye methylene blue. Superior photocatalytic activity of surface-modified TiO 2 NFs with RhA is attributed to their enhanced optical properties, i.e., the ability to harvest the photon energy in the visible spectral range.
✓ 已验证
DOI
WATER AIR AND SOIL POLLUTION
·
2016
·Vol. 227
·期 8
·1-9
·
IF 1.551
Yu Yanghai,Zhou Shiqing,Bu Lingjun,Shi Zhou,Zhu Shumin
An electrochemically activated persulfate (EC/PS) system was proposed for the degradation of herbicide diuron in this study. In the EC/PS system, the ferrous ions (Fe 2+ ) produced from iron electrode can activate persulfate to generate sulfate radical (SO 4
·- ) as well as hydroxyl radical (OH • ). The results showed that the degradation of diuron was significantly enhanced in the EC/PS system, compared to electrocoagulation, persulfate, and Fe 2+ /PS process. Both of SO 4
·- and OH · contributed to the degradation of diuron in the EC/PS system according to the radical scavenging studies. The pseudo first-order rate constants of diuron increased with increasing the applied currents and dosages of persulfate. pH affected the degradation of diuron indirectly through the speciation of iron and resulted in higher removal efficiency in acidic condition than in alkaline condition. Chloride, carbonate, and bicarbonate in real water inhibited the degradation of diuron dramatically through consuming SO 4
·- and OH · and abided by the order of CO 3
2− >HCO 3
− >Cl − . This study demonstrates that the EC/PS system is a novel, efficient, promising, and environmental-friendly method to treat diuron contamination.
✓ 已验证
DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-15
·
IF 14.919
Xiong Pan,Zhang Fan,Zhang Xiuyun,Liu Yifan,Wu Yunyan,Wang Shijian,Safaei Javad,Sun Bing,Ma Renzhi,Liu Zongwen,Bando Yoshio,Sasaki Takayoshi,Wang Xin,Zhu Junwu,Wang Guoxiu
The regulation of anions and cations at the atomic scale is of great significance in membrane-based separation technologies. Ionic transport regulation techniques could also play a crucial role in developing high-performance alkali metal batteries such as alkali metal-sulfur and alkali metal-selenium batteries, which suffer from the non-uniform transport of alkali metal ions (e.g., Li + or Na + ) and detrimental shuttling effect of polysulfide/polyselenide anions. These drawbacks could cause unfavourable growth of alkali metal depositions at the metal electrode and irreversible consumption of cathode active materials, leading to capacity decay and short cycling life. Herein, we propose the use of a polypropylene separator coated with negatively charged Ti 0.87 O 2 nanosheets with Ti atomic vacancies to tackle these issues. In particular, we demonstrate that the electrostatic interactions between the negatively charged Ti 0.87 O 2 nanosheets and polysulfide/polyselenide anions reduce the shuttling effect. Moreover, the Ti 0.87 O 2 -coated separator regulates the migration of alkali ions ensuring a homogeneous ion flux and the Ti vacancies, acting as sub-nanometric pores, promote fast alkali-ion diffusion.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）