Acta Biomaterialia
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2024
·
IF 9.4
Hongyi Lv,Ruijuan Xu,Xiangyan Xie,Qianqian Liang,Wanting Yuan,Yuting Xia,Xue Ao,Shiqiao Tan,Lijuan Zhao,Jinrong Wu,Yi Wang
The integration of barrier materials with pharmacological therapy is a promising strategy to treat intrauterine adhesions (IUAs). However, most of these materials are surgically implanted in a fixed shape and incongruence with the natural mechanical properties of the uterus, causing poor adaptability and significant discomfort to the patients. Herein, an injectable, biodegradable, and mechanically adaptive hydrogel loaded with platelet-rich plasma (PRP) is created by L‑serine and allyl functionalized chitosan (ACS) to achieve efficient, comfortable, and minimally invasive treatment of IUAs. L‑serine induces fast gelation and mechanical reinforcement of the hydrogel, while ACS introduces, imparting a good injectability and complaint yet strong feature to the hydrogel. This design enables the hydrogel to adapt to the complex geometry and match the mechanical properties of the uterine. Moreover, the hydrogel exhibits proper degradability, sustained growth factors (GFs) of PRP release ability, and good biocompatibility. Consequently, the hydrogel shows promising therapeutic efficacy by reducing collagen fiber deposition and facilitating endometrium cell proliferation, thereby restoring the fertility function of the uterus in an IUAs model of rats. Accordingly, the combination of L‑serine and ACS-induced hydrogel with such advantages holds great potential for treating IUAs. Statement of significance This research introduces a breakthrough in the treatment of intrauterine adhesions (IUAs) with an injectable, biodegradable and mechanically adaptive hydrogel using L‑serine and allyl functionalized chitosan (ACS). Unlike traditional surgical treatments, this hydrogel uniquely conforms to the uterus's geometry and mechanical properties, offering a minimally invasive, comfortable, and more effective solution. The hydrogel is designed to release growth factors from platelet-rich plasma (PRP) sustainably, promoting tissue regeneration by enhancing collagen fiber deposition and endometrium cell proliferation. Demonstrated efficacy in a rat model of IUAs indicates its great potential to significantly improve fertility restoration treatments. This advancement represents a significant leap in reproductive medicine, promising to transform IUAs treatment with its innovative approach to achieving efficient, comfortable, and minimally invasive therapy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xiangyan Xie,Xue Ao,Ruijuan Xu,Hongyi Lv,Shiqiao Tan,Jinrong Wu,Lijuan Zhao,Yi Wang
The combination of pharmacological and physical barrier therapy is a highly promising strategy for treating intrauterine adhesions (IUAs), but there lacks a suitable scaffold that integrates good injectability, proper mechanical stability and degradability, excellent biocompatibility, and non-toxic, non-rejection therapeutic agents. To address this, a novel injectable, degradable hydrogel composed of poly(ethylene glycol) diacrylate (PEGDA), sodium alginate (SA), and l -serine, and loaded with platelet-rich plasma (PRP) (referred to as PSL-PRP) is developed for treating IUAs. l -Serine induces rapid gelation within 1 min and enhances the mechanical properties of the hydrogel, while degradable SA provides the hydrogel with strength, toughness, and appropriate degradation capabilities. As a result, the hydrogel exhibits an excellent scaffold for sustained release of growth factors in PRP and serves as an effective physical barrier. In vivo testing using a rat model of IUAs demonstrates that in situ injection of the PSL-PRP hydrogel significantly reduces fibrosis and promotes endometrial regeneration, ultimately leading to fertility restoration. The combined advantages make the PSL-PRP hydrogel very promising in IUAs therapy and in preventing adhesions in other internal tissue wounds.
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NanoImpact
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2024
·
IF 4.7
Nan Zhao,Chang Liu,Chenyang Ji,Xuefeng Jiang,Jinyu Zhao,Liwen Qiang,Hangbiao Jin
Titanium dioxide nanoparticles (TiO 2 NPs) have been widely used in agriculture, which increased the risk to soil-plant systems. Studies have demonstrated that TiO 2 NPs can induce phytotoxicity. However, the toxicity mechanisms, particularly under the stress of TiO 2 NPs with different crystalline forms, remain inadequately reported. In this study, we combined transcriptomics and metabolomics to analyze the toxicity mechanisms in rice ( Oryza sativa L.) under the stress of anatase (AT) or rutile (RT) TiO 2 NPs (50 mg/kg, 40 days). The length (decreased by 1.1-fold, p = 0.021) and malondialdehyde concentration (decreased by 1.4-fold, p = 0.0027) of rice shoots was significantly reduced after AT exposure, while no significant changes were observed following RT exposure. Antioxidant enzyme activities were significantly altered both in the AT and RT groups, indicating TiO 2 NPs induced rice oxidative damage (with changes of 1.1 to 1.4-fold, p < 0.05). Additionally, compared to the control, AT exposure altered 3247 differentially expressed genes (DEGs) and 56 significantly differentially metabolites in rice (collectively involved in pyrimidine metabolism, TCA cycle, fatty acid metabolism, and amino acid metabolism). After RT exposure, 2814 DEGs and 55 significantly differentially metabolites were identified, which were collectively involved in fatty acid metabolism and amino acid metabolism. Our results indicated that AT exposure led to more pronounced changes in biological responses related to oxidative stress and had more negative effects on rice growth compared to RT exposure. These findings provide new insights into the phytotoxic mechanisms of TiO 2 NPs with different crystalline forms. Based on the observed adverse effects, the study emphasizes that any form of TiO 2 NPs should be used with caution in rice ecosystems. This study is the first to demonstrate that AT is more toxic than RT in paddy ecosystems, providing crucial insights into the differential impacts and toxic mechanisms of TiO 2 NPs with different crystalline forms. These findings suggest prioritizing the use of RT when TiO 2 NPs are necessary in agricultural development to minimize toxicity risks.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lian Xu,Jun-Jiang Shen,Ming Wu,Bing-Mei Su,Xin-Qi Xu,Juan Lin
Norepinephrine, a kind of β-adrenergic receptor agonist, is commonly used for treating shocks and hypotension caused by a variety of symptoms. The development of a straightforward, efficient and environmentally friendly biocatalytic route for manufacturing norepinephrine remains a challenge. Here, we designed and realized an artificial biocatalytic cascade to access norepinephrine starting from 3, 4-dihydroxybenzaldehyde and L-threonine mediated by a tailored-made L-threonine transaldolase PsLTTA-Mu1 and a newly screened tyrosine decarboxylase ErTDC. To overcome the imbalance of multi-enzymes in a single cell, engineering of PsLTTA for improved activity and fine-tuning expression mode of multi-enzymes in single E.coli cells were combined, leading to a robust whole cell biocatalyst ES07 that could produce 100 mM norepinephrine with 99% conversion, delivering a highest time-space yield (3.38 g/L/h) ever reported. To summarized, the current study proposed an effective biocatalytic approach for the synthesis of norepinephrine from low-cost substrates, paving the way for industrial applications of enzymatic norepinephrine production.
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Acta Biomaterialia
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2016
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IF 6.008
Qianming Lin,Yumeng Yang,Qian Hu,Zhong Guo,Tao Liu,Jiake Xu,Jianping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
Hydrogels have attracted much attention in cancer therapy and tissue engineering due to their sustained gene delivery ability. To obtain an injectable and high-efficiency gene delivery hydrogel, methoxypolyethylene glycol (MPEG) was used to conjugate with the arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) by click reaction, and then the synthesized MPEG-PLLD-Arg interacted with α-cyclodextrin (α-CD) to form the supramolecular hydrogel by the host-guest interaction. The gelation dynamics, hydrogel strength and shear viscosity could be modulated by α-CD content in the hydrogel. MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition. After gelation, MMP-9 shRNA plasmid (pMMP-9) could be encapsulated into the hydrogel matrix in situ and be released from the hydrogels sustainedly, as the release rate was dependent on α-CD content. The released MPEG-PLLD-Arg/pMMP-9 complex still showed better transfection efficiency than PEI-25k and induced sustained tumor cell apoptosis. Also, in vivo assays indicated that this pMMP-9-loaded supramolecular hydrogel could result in the sustained tumor growth inhibition meanwhile showed good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system , this supramolecular hydrogel is a promising candidate for long-term gene therapy. Statement of Significance To realize the sustained gene delivery for gene therapy, a supramolecular hydrogel with high-efficiency gene delivery ability was prepared through the host-guest interaction between α-cyclodextrin and PEGylated arginine-functionalized poly( l -lysine) dendron. The obtained hydrogel was injectable and biocompatible with adjustable physicochemical property. More importantly, the hydrogel showed the high-efficiency and sustained gene transfection to our used cells, better than PEI-25k. The supramolecular hydrogel resulted in the sustained tumor growth inhibition meanwhile keep good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system, this supramolecular hydrogel is a promising candidate in long-term gene therapy and tissue engineering.
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CATALYSIS LETTERS
·
2024
·Vol. 155
·期 1
·1-10
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IF 2.3
Wang Xu,Li Guohua,Feng Guo,Wang Yajie,Sun Hongxin
In this study, we developed a multiphase catalyst, BNNSs/His-Pd, by anchoring palladium nanoparticles onto L-histidine-modified boron nitride nanosheets. BNNSs/His-Pd was shown to be a good 2D heterogeneous catalyst. The enhanced affinity of the modified BNNSs for metal complexes, coupled with their inherent stability, contributes to the catalytic activity of the BNNSs/His-Pd-catalyzed Suzuki coupling reaction. The catalyst showed a broad substrate scope, good catalytic activity, and the convenience of not requiring inert gas protection in the Suzuki–Miyaura cross-coupling reaction. Even after 10 recycling cycles, the product yield remained at 95%, highlighting its significant potential for practical applications. Graphical Stable and efficient BNNSs/His-Pd heterogeneous catalysts were successfully constructed by modifying boron nitride nanosheets with L-histidine. The effect of L-histidine enhanced the dispersion and stability of Pd NPs on BNNSs carriers, combined with the inherent stability of BNNSs which resulted in the improvement of catalytic activity and recycling performance of the catalysts in the Suzuki coupling reaction.
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Chinese Medicine
·
2024
·Vol. 19
·期 1
·1-24
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IF 5.3
Cao Ting,Wang Xue-lian,Rao Jiang-yan,Zhu Hui-feng,Qi Hong-yi,Tian Zhen
Background Parkinson’s disease (PD) is a chronic neurodegenerative disorder that currently has no curable strategies. More and more evidence suggests that endoplasmic reticulum (ER) stress plays an essential role in PD pathogenesis. Periplaneta americana L. ( P. americana ) is a traditional Chinese medicine with diverse therapeutic properties. This study aims to investigate the neuroprotective effect and underlying mechanism of P. americana in in vitro and in vivo PD models. Methods The exposure of SH-SY5Y cells to 1-methyl-4-phenyl-pyridinium (MPP + ) was used as the in vitro PD model. MTT assay, Hoechst staining, Calcein AM-PI staining and flow cytometry were performed to measure the cell viability and apoptosis. DCFH-DA and JC-1 assay were used to measure the intracellular ROS and mitochondrial membrane potential (Δψm), respectively. Western-blot and immunostaining were conducted to detect the expression of key molecules related with ER stress. For the in vivo PD model induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydro-pyridine (MPTP), the motor function of mice was assessed by behavioral tests, the level of TH was examined by western-blot and immunostaining, the expression of key molecules related with ER stress was measured by western-blot. Results Periplaneta americana ethanol extract (PAE) concentration-dependently inhibited MPP + -induced cell loss and increased cell viability. PAE also remarkably attenuated ROS accumulation, the decline of Δψm as well as the excessive ER stress. The neuroprotective effects of PAE could be blocked by ROS inducer trimethylamine N-Oxide or ER stress activator tunicaymycin, while the antioxidant N-Acetyl-L-cysteine or ER stress inhibitor sodium 4-phenylbutyrate mimicked the effects of PAE. Furthermore, we found that PAE could activate AKT/GSK3β/β-catenin pathway. The effect of PAE on ROS production, Δψm and ER stress was blocked by AKT inhibitor MK-2206. In in vivo model, PAE significantly improved motor function, prevented dopaminergic neuronal loss and attenuated ER stress in substantia nigra and striatum of MPTP-treated mice. Similarly, the effects of PAE on MPTP-treated mice were also abolished by MK-2206. Conclusions Our results suggest that P. americana exerts neuroprotective effects through inhibiting ER stress via AKT-dependent pathway. Periplaneta americana may represent a promising therapeutic agent for PD treatment and is worthy of further being exploited.
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Analytical Methods
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2024
·
IF 2.7
Wenjuan Wang,Danrong Chen,Yujiao Cai,Zijing Liu,Hongfen Yang,Hongbin Xie,Jinquan Liu,Shengyuan Yang
Herein, we presented a paper-based POCT sensor based on lactate dehydrogenase-mediated alginate gelation combined with visual distance reading and smartphone-assisted colorimetric analysis dual-signal to determine the concentrations of L-lactate in yogurt samples. In this research, L-lactate was transformed to pyruvate by lactate dehydrogenase. The pyruvate then triggered the gelation of the sol mixture, increasing the viscosity (ηs) of the mixture, which showed a decrease in diffusion diameter on the paper-based sensor. Also, protons from pyruvate accelerated the degradation of Rhodamine B, causing color fading of the mixture, which could be analyzed by a RGB analysis application software. Under the optimal experimental conditions, the linear ranges of the visual distance reading and smartphone-assisted colorimetric analysis were 0.1-15 μM for the former and 0.3-15 μM for the latter, and the detection limits were 0.03 μM and 0.07 μM, respectively. As a proof-of-concept application, we then exploited the paper-based sensor to determine the concentrations of L-lactate in yogurt samples. The results from the dual-signal paper-based sensor are consistent with the ones from HPLC analysis. In short, this study developed a simple, convenient, cost-effective and feasible method for the quantitative detection of L-lactate in real samples.
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FOOD HYDROCOLLOIDS
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2024
·
IF 11
Xuemin Wang,Chaofan Guo,Jing Liu,Xianchao Feng,Jiliang Zhang,Jianxin Cao,Xuejiao Wang,Tingting Feng
The study utilized the synergistic effect of l -lysine and K + to improve the stability of Pickering emulsions based on κ -carrageenan microgels. The gel strength of κ -carrageenan gels, the zeta potential and particle size revealed that the microgel particles prepared with l -lysine and K + had the potential to be Pickering particles. l -lysine formed electrostatic interactions and hydrogen bonds with κ -carrageenan. Structural modifications of microgel particles by l -lysine (0.05%–0.10%) and low K + strength (≤40 mmol/L) promoted the formation of a dense interface layer at the oil-water droplet surface to prevent the accumulation of oil droplets and further significantly enhance the appearance and centrifugal stability of Pickering emulsions. The microstructure and rheological analysis showed that K + at low ionic strength, in cooperation with l -lysine, formed a more tightly structured layer between the microgel particles wrapped on the surface of oil droplets, thereby enhancing the stability of emulsions. In conclusion, these Pickering emulsions with prolonged stability are expected to be suitable healthy animal fat substitutes for use in food applications.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2024
·
IF 3.8
Jingwen Qian,Susu Liu,Zhongyu Wang,Mingyi Yu,Zheng Lian,Shule Zhang,Qin Zhong
In the typical wet flue gas denitration technology using sulfite solutions, the rapid free radical chain reaction of SO3•– oxidation by O2 is the main reason for the significant consumption of absorbents and inefficient denitration. The study utilizes l-ascorbic acid (VC) to inhibit the free radical chain reaction. VC combined with SO3•– to form stable compounds, which hindered the oxidation of SO3•– to SO5•– by O2, leading to an 80.7 mol % decrease in Na2SO3 consumption by adding millimolar-level VC. Compared to the sulfite solution, the best denitration efficiency of the VC/Na2SO3 mixture solution increased from 94.4 to 99.2 vol %, and the absorption time for absorption efficiency above 90 vol % was extended from 7 to 72 min. Additionally, a macro absorption model describing the rates of macro absorption was established based on various operating conditions.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Qianqian Jiang,Rongrong Lu,Zhihong Ma,Zhaoying He,Wanfu Hou,Mengtong Han,Pan Wang,Xiaoyan Zhao,Dan Wang
The microbial safety and quality properties of fresh-cut peppers ( Capsicum annuum L.) are influenced by disinfection. In this study, the effects of disinfection on the microbial community composition and quality attributes of green peppers stored at 4 ℃ for 8 days were investigated. The findings revealed that disinfection effectively reduced the total bacterial counts to approximately 6 log CFU/g at the end of storage compared to undisinfection samples (>7 log CFU/g) and altered bacterial community composition. Moreover, disinfection suppressed adverse changes in texture, maintained color by delaying chlorophyll degradation, and inhibited the accumulation of 12 screened key volatile organic compounds (VOCs) that produced mixed undesirable flavors, such as 2-hexenal and (E, E)-2,4-decadienal. Through the correlation analysis of microbiota and quality indicators, Pseudomonas was considered a marker of quality deterioration because of its significant negative correlation with firmness and positive correlations with ΔE and odor-causing VOCs, and disinfection decreased the relative abundance of this bacterium over time. These findings will provide vital guidance for fresh-cut pepper practices.
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PROGRESS IN ORGANIC COATINGS
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2024
·
IF 6.5
Mengchen Xue,Xiang Rao,Weihu Li,Jinghua Du,Wenhe Guo,Wangqin Zhou,Xiaoyu Dong,Le Wu,Guobing Zhang,Yunsheng Ding
A wide application of bio-based waterborne polyurethanes (BWPU) in the synthetic leather is the main direction in the future, however, it has been a great challenge for its development because of the poor mechanical properties and water resistance of BWPU. To address this issue, bio-based poly(trimethylene carbonate) diols (PTMC-OH) with a theoretical molecular weight of 2000 g/mol was prepared via ring-opening polymerization of trimethylene carbonate and 1,4-butanediol as an initiator. PTMC-OH, bio-based l -lysine diisocyanate as building blocks, and trimethylolpropane as a crosslinking agent were employed to prepare a series of linear and crosslinked bio-based waterborne polyurethanes (LBWPU and CBWPU). Remarkably, CBWPU-1 % film with crosslinked structure had high tensile strength and toughness shown as 41.5 MPa and 131 MJ·m −3 , respectively, while it also showed excellent hydrolysis resistance with the ignorable degradation in aqueous solution after 35 days since the weight loss of about 1.4 wt%. In addition, the synthetic leather coating made from CBWPU-1 % exhibited exceptional wear resistance, enduring 50,000 rubs without damages on the CBWPU coating. This work presents a facile and efficient method for the synthesis of BWPU and applying them to manufacture the bio-based synthetic leather.
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危险品化学品经营许可证（带存储）