Applied Sciences-Basel
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2021
·Vol. 11
·期 24
·11815
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IF 2.679
Meiwen Lv,Xiukang Wang,Noman Walayat,Zhongli Zhang,Muhammad Hamzah Saleem,Asad Nawaz,Rana Muhammad Aadil,Shakeel Ahmed,Mario J. Simirgiotis,José M. Lorenzo,Basit Latief Jan
This study aimed to analyze the cryoprotective effect of a ovalbumin (OVA) and β-cyclodextrin (βCD) mixture (3:1, OVA/βCD) on the structure, rheology and gelling properties of myofibrillar proteins (MPs) during 90 days of frozen storage. A mixture of OVA/βCD at different concentrations (0, 2, 4, and 6%) was added to MPs and stored at −18 °C for 90 days. The addition of OVA/βCD significantly decreased the sulfhydryl contents while it increased the surface hydrophobicity, which was closely connected with tertiary structural changes. Circular dichroism analysis showed that the addition of OVA/βCD enhanced the stability of the secondary structure by inhibiting the decline in the α-helix. Rheological properties analysis indicated that 6% OVA/βCD treatment showed better storage modulus (G’) and loss modulus (G”). In addition, treatment of OVA/βCD showed better gel forming properties than the control group (0%), helping to form a homogeneous and denser gel network. The results proved that 6% OVA/βCD could be act as a promising cryoprotectant, which can improve the structure and gel behavior ofCulter alburnusMPs during frozen storage. Moreover, OVA/βCD could be a potential alternative to conventional cryoprotectants at the industrial level to increase the economic and commercial values of seafood products.Keywords:myofibrillar proteins;ovalbumin;β-cyclodextrin;cryoprotectant;frozen storage
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MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Wei Wei,Qiang Han,Sheng Tian,Yanping Wang,Huiying Zhang,Haibo Wang,Qionglin Liang
The separation of active ingredients in traditional Chinese medicine (TCM) is of great significance in determining the efficacy of TCM as well as the pharmacological study of active ingredients, improving the scope of application of TCM and the efficiency of disease prevention and treatment. In the current study, we have successfully synthesized a core–shell structure magnetic microsphere Fe 3 O 4 -COF for the separation of α-asarone and β-asarone in the combined decoction of Acorous Tatarinowii Rhizoma (ATR) and Polygalae Radix (PR). The successful synthesis of Fe 3 O 4 -COF was demonstrated chemically and morphologically by using five characterizations such as Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM). Extraction conditions were investigated and optimized, including adsorbent dosage, adsorption time, sample pH value, elution solvent, elution time and volume of elution solution. Under the optimal experimental conditions, we got a low limit of detection (LOD) of α-asarone and β-asarone were 0.0379 μg/mL and 0.0432 μg/mL, and the relative standard deviations (RSD) were less than 2.3%. And the number and intensity of impurity peaks in the chromatogram after extraction of complex samples were significantly reduced and the good recoveries were still maintained after multiple extractions of complex samples, which showed satisfied specific adsorption and excellent resistance to matrix interference. The successful establishment of this method will provide a new direction for the separation and analysis of active ingredients in traditional Chinese medicine.
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ACTA BIOCHIMICA POLONICA
·
2021
·Vol. 68
·期 4
·765-774
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IF 2.149
Shaoyi Li,Yong Li,Bibo Tan,Zhaojie An
Krüppel-like factor 1 (KLF1) is a transcription factor that exhibits promoting effect in cervical cancer, but its correlation with gastric cancer (GC) has not been reported yet. In this study, we explored the role and potential mechanism of KLF1 in GC progression by using a series of experimental methods including RT-qPCR, Western blot, CCK-8 assay, EdU staining, and cell cycle analysis. KLF1 was found to be elevated in GC tissues (n=415) compared with the normal tissues by applying UALCAN to analyze datasets from The Cancer Genome Atlas (TCGA). The upregulation of KLF1 was also validated in GC cell lines. Functional studies proved that RNA interference-mediated silencing of KLF1 inhibited GC cell growth, as evidenced by the decreased cell viability, DNA synthesis, and arrested cell cycle in G1 phase. Moreover, KLF1 knockdown exerted the inhibitory effects on cell migration and invasion as well as the epithelial-mesenchymal transition (EMT) in GC cells. Conversely, overexpression of KLF1 had the opposite effects on GC progression. Furthermore, we proved that the activation of Wnt/β-catenin pathway was markedly inhibited by KLF1 knockdown and promoted by KLF1 overexpression. The blockade of Wnt/β-catenin pathway rescued the effects of KLF1 overexpression. These results suggested that KLF1 promoted the growth, migration, invasion, and EMT process in GC cells, and this promotion was achieved by activating the Wnt/β-catenin pathway. This work will be helpful for searching the potential therapeutic targets for treatment of GC.
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FOOD HYDROCOLLOIDS
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2021
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IF 9.147
Zhi-Wei Liu,Ying-Xue Zhou,Lang-Hong Wang,Zhang Ye,Lian-Jie Liu,Jun-Hu Cheng,Feng Wang,Alaa El-Din Bekhit,Rana Muhammad Aadil
In this study, interaction mechanism of astaxanthin (AST) with β-lactoglobulin (β-La) nanodispersions that were prepared by emulsification-evaporation method was investigated using multi-spectroscopies and molecular docking. Further, the antioxidant activity of the resultant nanodispersions was evaluated. The AST was successfully bound to the β-La and spontaneous interaction between β-La and AST complex was established. Hydrophobic interactions between β-La and AST were the dominant force that maintained the binding affinity. The nanodispersions were characterized by a static quenching of intrinsic fluorescence of the β-La, an increased particle size of β-La and AST complex and a decreased surface hydrophobicity of β-La. Molecular docking investigation of the nanodispersions elucidated that a linear molecule of AST was partly inserted into the hydrophobic core of β-La, which located at the internal cavity of β-barrel of β-La and encompassed by hydrophobic amino acid residues. The binding between β-La and AST significantly (P < 0.05) enhanced 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activity and intracellular reactive oxygen species scavenging ability of AST. The ABTS scavenging ability of AST in β-La/AST nanodispersion was almost four-fold higher than that of the individual AST. This study provided evidence that suggests the incorporation of AST in dairy products could have great potential to enhance the functionality of the dairy products and convey better activity than the individual components.
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FOOD CHEMISTRY
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2021
·
IF 7.514
Runan Zhao,Weiting Fu,Yijie Chen,Bin Li,Shilin Liu,Yan Li
The effect of cinnamaldehyde (CA) on the structure and properties of whey protein isolate (WPI) was investigated. The resultant WPI/CA complex was used as stabilizer to form emulsions and emulsion gels, which were used for the delivery and protection of β-carotene. The particle size and hydrophobicity of WPI solution increased and then decreased with the addition of CA. Circular dichroism showed that CA mainly changed the secondary structure of WPI, with increasing β-fold content from 47.2% to 72.9%. The fluorescence spectra showed that both tryptophan and tyrosine in WPI were involved in the interaction with CA. WPI/CA complex as the stabilizer could form the stable emulsions and emulsion gels, which showed better protection effect on β-carotene, and helped enhance its bioaccessibility. The knowledge provides insights into the development of new multifunctional food ingredients and the enhancement of protein modification in food system.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Shuai Lin,Changjun Zou,Hao Liang,Hong Peng,Ya Liao
β-cyclodextrin was covalently grafted onto the surface of magnetic multi-walled carbon nanotubes to synthesize a novel composite material (denoted as β-CD@Fe 3 O 4 /MWCNT). The structure of the magnetic material was characterized by using Fourier transform infrared (FT-IR spectroscopy), Raman spectroscopy , X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), vibrating sample magnetometer (VSM), and specific surface area (BET). The results from SEM showed that the dispersion of carbon nanotubes was better after β-cyclodextrin was compounded. TGA indicated that about 7.1% of the β-cyclodextrin was grafted onto the surface of the magnetic carbon nanotubes. In addition, β-CD@Fe 3 O 4 /MWCNT had larger specific surface area and pore volume . The adsorption of nickel ions on β-CD@Fe 3 O 4 /MWCNT was affected by pH, temperature and adsorption time. The pseudo-second-order kinetic model and Langmuir model describe the adsorption process well. Thermodynamic data showed that the adsorption process was spontaneous and exothermic, and the maximum adsorption amount of nickel ions (Ni 2+ ) on the β-CD@Fe 3 O 4 /MWCNT was 103 mg g −1 at room temperature . Moreover, regeneration experiments showed that the adsorbent still maintained a high adsorption capacity after 5 cycles. On this basis, the interaction mechanism between magnetic nanoparticles and Ni 2+ was discussed. In summary, as an environmentally friendly adsorbent, β-CD@Fe 3 O 4 /MWCNT has the potential to remove nickel ions from wastewater.
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POLYMER
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2021
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IF 4.43
Lin-feng Han,Xue Geng,Lin Ye,Ai-ying Zhang,Zeng-guo Feng
In this study β-cyclodextrins (β-CDs) were reacted with 1,6-diisocyanatohexane (HDI) end-capped poly (ε-caprolactone) (PCL) diols (M n = 10–80 kg/mol) to give access to β-CD terminated PCLs (PCL-CDs). At the same time an adamantine terminated PCL (P2Ad) was synthesized from the reaction of a PCL diol (M n = 2 kg/mol) with 1-adamantyl isocyanate and an adamantine end-capped hexane (HAd) was prepared from the reaction of HDI with 1-adamantyl amine. Subsequently PCL-CDs and their mixtures with HAd and P2Ad at a 95:5 feeding mass ratio were dissolved in hexafluoroisopropanol and cast into films. The dried films were characterized by means of mechanical property, shape memory, DSC and DMA measurements to reveal their phase-separation mechanism and solvent-free inclusion complexation. It was shown that attaching β-CDs to two ends of PCL diols leads to the new phase-separation morphology in the PCL-CDs matrix making a great contribution to the mechanical and shape memory properties. Adding HAd and P2Ad further enhances the mechanical and shape memory properties evidencing the occurrence of solvent-free inclusion complexation between β-CD and Ad-terminated PCLs.
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Drug Design Development and Therapy
·
2021
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IF 4.162
Weiyong Hong,Fangyuan Guo,Nan Yu,Sanjun Ying,Bang Lou,Jiangqing Wu,Ying Gao,Xugang Ji,Haiying Wang,Aiqin Li,Guoping Wang,Gensheng Yang
Purpose A novel folate receptor-targeted β-cyclodextrin (β-CD) drug delivery vehicle was constructed to improve the bioavailability, biosafety, and drug loading capacity of curcumin. Controlled release and targeted delivery was achieved by modifying the nanoparticles with folic acid (FA).Methods Folate-conjugated β-CD-polycaprolactone block copolymers were synthesized and characterized. Curcumin-loaded nanoparticles (FA-Cur-NPs) were structured by self-assembly. The physicochemical properties, stability, release behavior and tumor-targeting ability of the fabricated nanoparticles were studied.Results The average particle size and drug loading of FA-Cur-NPs was 151.8 nm and 20.27%, respectively. Moreover, the FA-Cur-NPs exhibited good stability in vitro for 72 h. The drug release profiles showed that curcumin from FA-Cur-NPs was released significantly faster in a pH 6.4 phosphate buffered solution (PBS) than in pH 7.4, indicating that curcumin can be enriched around the tumor site compared with normal cells. Additionally, the internalization of FA-Cur-NPs was aided by FA receptor-mediated endocytosis, and its cytotoxicity was proportional to the cellular uptake efficiency. Furthermore, in vivo studies confirmed that FA-Cur-NPs exhibited marked accumulation in the tumor site and excellent antitumor activity.Conclusion These findings suggest that FA-Cur-NPs are a promising approach for improving cancer therapy through active targeting and controllable release.
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ACTA HISTOCHEMICA
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2021
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IF 2.479
Xue Liu,Yumeng Shao,Xinyue Zhang,Xiang Ji,Min Xie,Huaman Liu
The precise etiology and pathogenesis of idiopathic pulmonary fibrosis are not completely understood, and no satisfactory treatment exists. This work aimed to examine the effects of calycosin (CA, an isoflavone compound) on pulmonary fibrosis (PF) and explore the underlying mechanism. In this study, we established a mice model of PF induced by 5 mg/mL bleomycin (BLM), and mice were orally administrated with 7 mg/kg or 14 mg/kg CA once a day for three weeks. In vitro , after pretreated with 80 μM CA, MLE-12 cells were stimulated with 10 ng/mL transforming growth factor-β1 (TGF-β1) for inducing epithelial-mesenchymal transition (EMT). The results showed that CA treatment ameliorated the severity of fibrosis and the lung tissue damage, as well as suppressed the secretion of inflammation factors in a dose-dependent manner of the PF mice model induced by BLM. Subsequently, CA inhibited the BLM-induced PF progression by repressing EMT, evidenced by the reverse of the downregulation of E-cadherin and the upregulation of vimentin, α-SMA, and fibronectin. Moreover, the elevated phosphorylation of AKT and GSK3β induced by BLM (or TGF-β1) was decreased by CA treatment, leading to the rescue of the high expression of β-catenin. CA prevented the translocation of β-catenin from the cytoplasm to the nucleus. The repressed effects of CA on the TGF-β1-induced EMT and the AKT/GSK3β/β-catenin axis, as well as the translocation of β-catenin were all reversed by a AKT activator SC79. Taken together, CA ameliorated PF by the EMT inhibition upon suppressing the AKT/GSK3β/β-catenin signaling pathway.
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Materials Today Communications
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2021
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IF 3.383
Jinxing Song,Huafeng Feng,Mengqi Wu,Linxiao Chen,Wei Xia,Wenqing Zhang
Biodegradable membranes of chitosan and its composite with other natural polymers provide a promising solution for biomaterials. To further improve the antibacterial activities of chitosan (CS)/hydroxypropyl methyl cellulose (HPMC) membrane, natural extracts tea polyphenols (TP) were employed in this work. And TP was encapsulated by β -cyclodextrin (CD) beforehand to improve its compatibility with HPMC. To our knowledge, it’s the first time ever reported to use CD encapsulated TP in CS/HPMC membranes. And variety characterization results such as Fourier transform infrared spectroscopy (FTIR), ultraviolet spectra (UV) and dynamic light scattering (DLS) showed that TP had successfully encapsulated with CD. After the solvent blending of CS/HPMC and CDTP, CS/HPMC/CDTP membrane was prepared by a casting method and exhibited good toughness and tensile strength. Finally, bacterial inhibitive rate and inhibition zones of the membrane with E. coli and S. aureus proved the enhanced antibacterial as expected. And the morphology of the bacteria was observed to estimate the possible interaction approach of materials and bacteria. The novel biodegradable membrane can be used for antibacterial coating application.
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PHYTOMEDICINE
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2021
·
IF 5.34
Yongmei Li,Zean Zhao,Jian Luo,Yanqing Jiang,Lu Li,Yanyu Chen,Leqi Zhang,Qinghua Huang,Ying Cao,Pingzheng Zhou,Ting Wu,Jianxin Pang
Background Hyperuricemia (HUA) is characterized by abnormal serum uric acid (UA) levels and demonstrated to be involved in renal injury leading to hyperuricemic nephropathy (HN). Apigenin (API), a flavonoid naturally present in tea, berries, fruits, and vegetables, exhibits various biological functions, such as antioxidant and anti-inflammatory activity. Purpose To investigate the effect of API treatment in HN and to reveal its underlying mechanisms. Methods The mice with HN were induced by potassium oxonate intraperitoneally and orally administered for two weeks. The effects of API on renal function, inflammation, fibrosis, and uric acid (UA) metabolism in mice with HN were evaluated. The effects of API on urate transporters were further examined in vitro . Results The mice with HN exhibited abnormal renal urate excretion and renal dysfunction accompanied by increased renal inflammation and fibrosis. In contrast, API reduced the levels of serum UA, serum creatinine (CRE), blood urea nitrogen (BUN) and renal inflammatory factors in mice with HN. Besides, API ameliorated the renal fibrosis via Wnt/β-catenin pathway suppression. Furthermore, API potently promoted urinary UA excretion and inhibited renal urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) in mice with HN. In vitro , API competitively inhibited URAT1 and GLUT9 in a dose-dependent manner, with IC 50 values of 0.64 ± 0.14 μM and 2.63 ± 0.69 μM, respectively. Conclusions API could effectively attenuate HN through co-inhibiting UA reabsorption and Wnt/β-catenin pathway, and thus it might be a potential therapy to HN.
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JOURNAL OF FOOD SCIENCE
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2021
·Vol. 86
·期 5
·2118-2130
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IF 3.167
Lingyu Zhu,Yang Song,Huilin Liu,Min Wu,Haizhou Gong,Hainan Lan,Xin Zheng
β-Carotene displays antioxidant and anti-inflammatory activities and prevents the development of cancer. Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer. However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear. In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS). The results revealed that β-carotene significantly ( p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged. Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation. β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00). The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats. Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium , the levels of which negatively correlated with the levels of inflammatory cytokines. Faecalibacterium may be a potential target in the alleviation of DSS-induced UC. β-Carotene can alleviate DSS-induced UC through the regulation of gut microbiota. This study provides a reference for the rational use of β-carotene in the treatment of UC. Practical Application β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
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