Biomolecules
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2020
·Vol. 10
·期 6
·825
·
IF 4.082
Yazhen Hu,Tomofumi Kurobe,Xiaoling Liu,Yong-An Zhang,Jianguo Su,Gailing Yuan
Hepcidin is an antimicrobial peptide and regulator of iron homeostasis which has two isoforms in most fishes and some mammals. Previous studies have reported that the two hepcidin isoforms have different roles. Hamp type-1 plays a regulatory role in iron metabolism and hamp type-2 mostly performs an antimicrobial role. In this study, we found thatCtenopharyngodon idella(C. idella) have only one hepcidin isoform (hamp type-1), which showed both broad-spectrum antibacterial and iron regulatory functions.C. idellahepcidin mature peptide (hepcidin-25) and truncated peptide (hepcidin-20) exhibited bactericidal activities against both Gram-positive and Gram-negative bacteria in a dose-dependent manner in part through membrane rupture and binding to bacterial genomic DNA. The data from challenge tests demonstrated that the administration of hepcidin-25 significantly reduced mortality rates ofC. idellabyA. hydrophilainfection, probably due to direct bactericidal activities of the peptide and a reduction of iron content in the fish serum. In addition, a comparison between hepcidin-20 and -25 suggests that the N terminal 5 amino acids play a critical role in reducing iron content in fish serum. Our findings revealed an important role of hamp type-1 in maintaining iron homeostasis and fighting against bacterial infections, suggesting the hepcidin has implications for the prevention and control of bacterial infection in aquaculture.Keywords:antibacterial;Ctenopharyngodon idella;hepcidin;host defense;iron homeostasis
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Jingming Wang,Bin Wu,Na Zhang,Mingyue Zhao,Tingting Jing,Yi Wu,YunQing Hu,Feng Yu,Xiaochun Wan,Wilfried Schwab,Chuankui Song
β-Ionone is a carotenoid-derived flavor and fragrance compound with a complex fruity and woody scent, known for its violet aroma. Due to the low odor threshold, β-ionone dramatically affects the aroma and quality of tea. Previous studies have shown that β-ionone increases during tea withering; however, its formation and regulation during the withering process are far from being understood. As dehydration is the most important stress during the withering of the tea leaves, we isolated a dehydration-induced gene belonging to the subfamily of carotenoid cleavage dioxygenases called carotenoid cleavage dioxygenase 1a (CsCCD1a) from Camellia sinensis and expressed it in Escherichia coli. The recombinant protein could generate volatile β-ionone and pseudoionone from carotenoids. CsCCD1a was induced by dehydration stress, and its expression was related to the β-ionone accumulation during tea withering. Overall, this study elucidated that CsCCD1a catalyzes the formation of β-ionone in C. sinensis and enhanced the understanding of the β-ionone formation under multiple stresses during the processing of tea.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Shuang Qiu,Jinghua Du,Yonghou Xiao,Qidong Zhao,Gaohong He
Ultra-deep removal of thiophene sulfides from transportation fuel via adsorption has aroused great attention under the pressure of gradually demanding environmental regulations. The development of high-efficiency adsorbents is the key to adsorptive desulfurization for producing clean transportation fuels. In this work, a microwave-assisted synthesis method was applied to rapidly fabricate hierarchical porous defective HKUST-1 (DEHKUST-1) towards removing benzothiophene from model fuel by selective adsorption. The effects of cetyltrimethylammonium bromide (CTAB) dosage, adsorption temperature and adsorption contact time on the DEHKUST-1 desulfurization performance were systematically investigated. The characterization results proved that both metal clusters and linker defects were generated in the as-prepared DEHKUST-1. CTAB as template could modulate the pore distribution and introduce reasonable defects in the framework of HKUST-1. At CTAB: Cu 2+ = 0.5 by mole ratio, a regular mesoporous distribution of 25–30 nm could be obtained with DEHKUST-1; it exhibited an impressive enhancement in adsorptive desulphurization performance. The maximum adsorption capacity of the adsorbent attained 14.4 mg S/g adsorbent at 20 °C in 4 h. Even after CTAB-H-0.5 was regenerated six times, its adsorption capacity remained 93% as that of the as-prepared adsorbent. An adsorption mechanism was proposed to illustrate the promoting desulfurization performance by introduction of hierarchical porous in HKUST-1. By virtue of the rapid synthesis and remarkable benzothiophene removal capacity, the DEHKUST-1 could be a potential adsorbent in desulfurization from real fuels.
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CATALYSIS TODAY
·
2025
·
IF 5.3
Yupeng Tian,Yujia Liu,Shoutao Ma,Wei Xu,Chenyang Zhao,Bing Sun
This study introduces a heart-like micromixer reactor and Au/TS-1 catalyst to address the dual challenges of safety and efficiency in gas-phase propylene epoxidation using H 2 and O 2 . Conventional methods face inherent explosion risks and rapid catalyst deactivation due to sintering and coking. The microreactor architecture achieves over 99% flame-quenching efficiency via sub-millisecond radical suppression and enhanced heat transfer, enabling safe operation with minimal inert gas dilution (N 2 < 70 vol%). Coupled with alkali-promoted Au/TS-1 catalysts, the system sustains > 90 % propylene oxide (PO) selectivity for 500 h, outperforming static mixers where activity halved within 100 h. This work pioneers a reactor-catalyst co-design strategy, decoupling safety constraints from reaction efficiency, and establishes a scalable platform for sustainable PO synthesis. Besides, the critical gaps in industrial-scale implementation of explosive gas-phase reactions is bridged.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2025
·
IF 14.3
Huan Zhou,Yulong Hao,Chen Fan,Shiwei Zhang,Chen Wang,Kaiyi Wang,Jie Zhou,Shijie Hao,Ting Shu,Xuemei Lu,Bo Li,Yongqiang Yu,Guolin Hao
Two-dimensional (2D) noble transition-metal dichalcogenide materials (NTMDs) have garnered remarkable attention due to their intriguing properties exhibiting potential applications in nanoelectronics, optoelectronics, and photonics. The electronic structure and physical properties of 2D NTMDs can be effectively modulated using alloy engineering strategy. Nevertheless, the precise growth of wafer-scale 2D NTMDs alloys remains a significant challenge. In this work, we have achieved the controllable preparation of wafer-scale (2-inch) 2D PdS 2 x Se 2(1 − x ) nanofilms (NFs) with fully tunable compositions on various substrates using pre-deposited Pd NFs assisted chemical vapor deposition technique . High-performance photodetectors based on the PdS 2 x Se 2(1 − x ) NFs were fabricated, which exhibit broadband photodetection performance from visible to near-infrared (NIR) wavelength range at room temperature. Significantly, the PdS 0.9 Se 1.1 -based photodetectors display a responsivity up to 0.192 A W −1 and a large specific detectivity of 5.5 × 10 11 Jones for 850 nm light, enabling an excellent high-resolution NIR single-pixel imaging (SPI) without an additional filtering circuit. Our work paves a new route for the controlled synthesis of wafer-scale and high-quality 2D NTMDs alloy NFs, which is essential for designing advanced optoelectronic devices.
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Protein & Cell
·
2024
·
IF 13.6
Ma Wei,Zhang Lu,Chen Weilong,Chang Zhaoxia,Tu Juchuanli,Qin Yuanyuan,Yao Yuwen,Dong Mengxue,Ding Jiajun,Li Siqin,Li Fengkai,Deng Qiaodan,Yang Yifei,Feng Tingting,Zhang Fanrong,Shao Xiying,He Xueyan,Zhang Lixing,Hu Guohong,Liu Quentin,Jiang Yi-Zhou,Zhu Shu,Xiao Zhi,Su Dan,Liu Tong,Liu Suling
Tumor-resident microbiota in breast cancer promote cancer initiation and malignant progression. However, targeting microbiota to improve the effects of breast cancer therapy has not been investigated in detail. Here, we evaluated the microbiota composition of breast tumors and found that enterotoxigenic Bacteroides fragilis (ETBF) was highly enriched in the tumors of patients who did not respond to taxane-based neoadjuvant chemotherapy. ETBF, albeit at low biomass, secreted the toxic protein BFT-1 to promote breast cancer cell stemness and chemoresistance. Mechanistic studies showed that BFT-1 directly bound to NOD1 and stabilized NOD1 protein. NOD1 was highly expressed on ALDH+ breast cancer stem cells (BCSCs) and cooperated with GAK to phosphorylate NUMB and promote its lysosomal degradation, thereby activating the NOTCH1-HEY1 signaling pathway to increase BCSCs. NOD1 inhibition and ETBF clearance increases the chemosensitivity of breast cancer by impairing BCSCs.
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JOURNAL OF HETEROCYCLIC CHEMISTRY
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2024
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IF 2
Guofang Jiang,Can Zhang,Xiaohong Xing,Jinbo Ouyang,Hong Yang,Zongbo Xie,Zhanggao Le
A novel and highly efficient method for the synthesis of 1-(4-hydroxybutyl)-2-benzoyl indoles from 2-pyrrolidine benzaldehyde and α -bromoacetophenone in a choline chloride (ChCl)/ZnCl 2 eutectic mixture was developed. This method, which does not require any catalyst, additive, or alkali, is useful for realizing tandem cyclization reactions. Several indoles were produced after cyclization and ring opening processes. Not all the compounds obtained are reported in the literature. This synthetic strategy proves that deep eutectic solvents can replace catalysts and organic solvents. Consequently, this is an environmentally friendly strategy that has significant potential in the development of green and sustainable chemistry.
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Chinese Journal of Natural Medicines
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2024
·
IF 4
Wenhui YAO,Rongpin TAO,Kai WANG,Xuansheng DING
ABSTRACT Icariin, a flavonoid glycoside, is extracted from Epimedium . This study aimed to investigate the vascular protective effects of icariin in type 1 diabetic rats by inhibiting high-mobility group box 1 (HMGB1)-related inflammation and exploring its potential mechanisms. The impact of icariin on vascular dysfunction was assessed in streptozotocin (STZ)-induced diabetic rats through vascular reactivity studies. Western blotting and immunofluorescence assays were performed to measure the expressions of target proteins. The release of HMGB1 and pro-inflammation cytokines were measured by enzyme-linked immunosorbent assay (ELISA). The results revealed that icariin administration enhanced acetylcholine-induced vasodilation in the aortas of diabetic rats. It also notably reduced the release of pro-inflammatory cytokines, including interleukin-8 (IL-8), IL-6, IL-1β, and tumor necrosis factor-alpha (TNF-α) in diabetic rats and high glucose (HG)-induced human umbilical vein endothelial cells (HUVECs). The results also unveiled that the pro-inflammatory cytokines in the culture medium of HUVECs could be increased by rHMGB1. The increased release of HMGB1 and upregulated expressions of HMGB1-related inflammatory factors, including advanced glycation end products (RAGE), Toll-like receptor 4 (TLR4), and phosphorylated p65 (p-p65) in diabetic rats and HG-induced HUVECs, were remarkably suppressed by icariin. Notably, HMGB1 translocation from the nucleus to the cytoplasm in HUVECs under HG was inhibited by icariin. Meanwhile, icariin could activate G protein-coupled estrogen receptor (GPER) and sirt1. To explore the role of GPER and Sirt1 in the inhibitory effect of icariin on HMGB1 release and HMGB-induced inflammation, GPER inhibitor and Sirt1 inhibitor were used in this study. These inhibitors diminished the effects of icariin on HMGB1 release and HMGB1-induced inflammation. Specifically, the GPER inhibitor also negated the activation of Sirt1 by icariin. These findings suggest that icariin activates GPER and increases the expression of Sirt1, which in turn reduces HMGB1 translocation and release, thereby improving vascular endothelial function in type 1 diabetic rats by inhibiting inflammation.
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Science of Advanced Materials
·
2016
·Vol. 8
·期 8
·1617-1627
·
IF 1.812
Wen Zhen,Su Jun-Qing,Chen Qing,Chen Li-Di
To improve the physicochemical and pharmacokinetic properties, solid lipid nanoparticles (SLNs) modified with (Polyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (PEG2000-DSPE) were prepared using glyceryl monostearate as a lipid carrier and resveratrol as a model
drug. Compared to ordinary SLNs, SLNs doped with low amount of PEG2000-DSPE [m(PEG2000-DSPE):m(lipids) = 1:5, w/w] exhibited improved physicochemical and pharmacokinetic properties, including higher entrapment efficiency (above 80%), smaller particle size (approximately 100 nm), more
distinguished drug release at low pH values (pH < 6.0), and longer drug circulation in vivo. According to the characterizations performed, the DSPE moiety was doped into lipid matrix to form a PEG2000-DSPE/lipid eutectic dopant, inhibiting lipid polymorphism transformation
from α-form to β-form, thereby preventing drug expulsion from the lipid. The dopant contained lattice defects, allowing for the incorporation of more resveratrol. Resveratrol was released in a sustained and pH-sensitive pattern due to nanoparticles destabilization
induced by partial protonation of PEG2000-DSPE. PEG chains distributed on the nanoparticle surface caused steric hindrance and “stealth” properties, thus maintaining dispersion stability and extending circulation time of nanoparticles. These results confirm that PEG2000-DSPE modification
effects are attributable to the matrix and surface structures of nanoparticles, which consist of a core containing DSPEinduced lattice defects and a shell coated with PEG chains.
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JOURNAL OF CHROMATOGRAPHY A
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2021
·
IF 4.759
Zhuoting Liu,Chunling Wang,Xu Wang,Ann Van Schepdael
Indoleamine 2,3-dioxygenase 1 (IDO1) has been shown to play an important role in the immune escape process of tumors, and therefore is considered as a promising target for tumor immunotherapy. In this study, off-line and on-line capillary electrophoresis methods were developed for IDO1 inhibitors screening from natural product extracts. The optimized separation conditions of CE were achieved with 32 mM sodium tetraborate (pH 9.22) as background electrolyte, using a separation voltage of 21 kV. The off-line CE method was verified by the determination of enzymatic kinetic parameters and inhibitory mechanisms of two known inhibitors. A partial filling on-line CE method combined with rapid polarity switching was used for rapid screening of IDO1 inhibitors. The whole reaction and separation process was completed within 5 min. The on-line CE screening results showed that six of 18 natural products had inhibitory effect on IDO1, namely Carthamus tinctorius, Schisandra chinensis , Raisin, Coffee, Hawthorn and Radix angelicae sinensis. The results of on-line CE experiments were consistent with the off-line results, which proved the practicability and effectiveness of the method for inhibitors screening.
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CARBOHYDRATE POLYMERS
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2021
·
IF 9.381
Zhixiang Cai,Hongbin Zhang
The chain conformational change in curdlan during carboxymethylation was investigated using nuclear magnetic resonance (NMR), circular dichroism (CD) spectroscopy, and atomic force microscopy (AFM). The distributions of carboxymethyl substituents within anhydroglucose unit (AGU) of CMCD were found to follow the order of OH (6) > OH (4) > OH (2) for CMCD with a low DS and OH (6) > OH (2) > OH (4) for CMCD with relatively high DS. The increased carboxymethylation level induced the chain conformation transition of curdlan from triple helix to random coil in water. The DS of 0.25 was the critical value of chain conformation transition, below which CMCD chains were triple helices. For DS larger than 0.25, CMCD existed in the state of random coils. The intermolecular hydrogen bonding between C2 hydroxyls in AGU sustained the triple helical conformation and stiffness of the polymer chain, which weakened with the increase in DS.
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ACS Nano
·
2024
·
IF 15.8
Xin Jiang,Lei Yi,Cheng Li,Haoxiang Wang,Wei Xiong,Yuan Li,Zaigang Zhou,Jianliang Shen
Currently, limited photosensitizers possess the capacity to reverse tumor hypoxia and reduce programmed death ligand-1 (PD-L1) and transforming growth factor-β (TGF-β) expression simultaneously, hindering the perfect photodynamic therapy (PDT) effect due to acquired immune resistance and the tumor hypoxic microenvironment. To tackle these challenges, in this research, we demonstrated that mitochondrial energy metabolism depression can be utilized as an innovative and efficient approach for reducing the expression of PD-L1 and TGF-β simultaneously, which may offer a design strategy for a more ideal PDT nanosystem. Through proteomic analysis of 5637 cells, we revealed that tamoxifen (TMX) can incredibly regulate PD-L1 expression in tumor cells. Then, to selectively deliver clinically used mitochondrial energy metabolism depressant TMX to solid tumors as well as design an ideal PDT nanosystem, we synthesized MHI-TMX@ALB by combining a mitochondria-targeted heptamethine cyanine PDT-dye MHI with TMX through self-assembly with albumin (ALB). Interestingly enough, the MHI-TMX@ALB nanoparticle demonstrated effective reversion of tumor hypoxia and inhibition of PD-L1 protein expression at a lower dosage (7.5 times to TMX), which then enhanced the efficacy of photodynamic immunotherapy via enhancing T-cell infiltration. Apart from this, by leveraging the heptamethine dye’s targeting capacity toward tumors and TMX’s role in suppressing TGF-β, MHI-TMX@ALB also more effectively mitigated 4T1 tumor lung metastasis development. All in all, the MHI-TMX@ALB nanoparticle could be used as a multifunctional economical PD-L1 and TGF-β codepression immune-regulating strategy, broadening the potential clinical applications for a more ideal PDT nanosystem.
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