Chemistry-Switzerland
·
2026
·Vol. 8
·期 1
·11
·
IF 2.4
Aikun Liu,Xu Liu,Zixuan Huang,Yanqing Ge
Nitrite, as a widely present nitrogen oxide compound in nature, and is extensively distributed in production and daily life; precise and rapid detection of it is of great significance for ensuring human health. This study developed nitrogen-doped carbon dots (N-CDs) using malic acid and 3-diethylaminophenol as precursors by one-step hydrothermal treatment. The obtained N-CDs exhibited strong green fluorescence with a high quantum yield of 20.86%. More importantly, they served as a highly effective fluorescent probe for NO2−sensing, demonstrating a low detection limit of 28.33 μM and a wide linear response range of 400 to 1000 μM. The sensing mechanism was attributed to an electrostatic interaction-enhanced dynamic quenching process. Notably, the probe enabled dual-mode detection: a distinct color change from light pink to dark brown under daylight for visual semi-quantification, and quantitative fluorescence quenching. The N-CDs showed excellent selectivity over common interfering ions. Furthermore, their low cytotoxicity and good biocompatibility allowed for successful bioimaging of exogenous and endogenous NO2−fluctuations in live HeLa cells. This work presents a facile green strategy to synthesize multifunctional N-CDs that realized the sensitive, selective, and visual detection of NO2−in environmental and biological systems.
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2026
·
IF 26.8
Weikang Lv,Tuya Naren,Abdellah Aazmi,Haoran Yu,Yujun Wang,Jie Ying Lee,Mengfei Yu,Junjun Jia,Xiuxiu Jiang,Huayong Yang,Liang Ma
Hepatic fibrosis, as the common pathological endpoint of chronic liver diseases, is characterized by a self-perpetuating vicious cycle comprising extracellular matrix (ECM) driven liver tissue stiffening and sustained hepatic stellate cell (HSC) activation. Although existing studies have simulated fibrotic microenvironments using 2D models with tunable matrix stiffness or static 3D cultures, these models lack engineered hepatic sinusoidal vasculature and dynamic mechanical stimulation within 3D ECM contexts. This study employed embedded sacrificial bioprinting to construct functional liver sinusoid-mimetic vascular networks within hydrogel matrix of precisely tunable elastic modulus, establishing a dynamically perfused in vitro liver fibrosis model. Experimental validation demonstrated that matrix stiffness directly drives HSC activation, inducing marked myofibroblastic transdifferentiation. Furthermore, compared to static models, 3D dynamic perfusion significantly enhanced hepatocyte sensitivity to high-stiffness matrix, more accurately replicating the functional decline of hepatocytes in fibrotic microenvironments observed in vivo. More critically, the biomimetic in vitro platform established in this study presents a potential avenue for evaluating pharmacotherapeutic interventions against liver fibrosis. Through targeted inhibition of key signaling hubs, we achieved partial reversal of HSC activation on stiff matrix and partial recovery of liver tissue function. Overall, by simultaneously integrating matrix stiffness modulation, 3D multicellular interactions, and hemodynamic stimulation, this work effectively addresses the insufficient responsiveness of hepatocytes to mechanical cues in conventional models due to inadequate mechanical stimulation. This approach provides a robust framework for faithfully recapitulating the pathophysiological progression of liver fibrosis in vitro through precise tuning of ECM mechanical properties, thereby offering a promising platform for future drug screening and therapeutic assessment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials
·
2026
·Vol. 19
·期 2
·445
·
IF 3.2
Ding Lin,Zhenhao Li,Jianying Hao,Xiaobo Xu,Xiuqiang Li,Yuan Feng,Xiaochen Lu,Fanglian Yao,Hong Zhang,Junjie Li
Diabetic wounds are often accompanied by severe inflammation, which is unfavorable for vascular growth and wound repair. Therefore, promoting the healing of diabetic wounds is of great significance. In this study, carboxymethyl chitosan (CMCS) was grafted with 4-formylphenylboronic acid (FPBA) and then crosslinked with oxidized sodium alginate (OAlg) to form a dual-dynamic covalent hydrogel (CPOA) based on borate ester bond and Schiff base bonds. Mesenchymal stem cells’ exosomes (Exos) were incorporated into the CPOA to construct CPOA@Exos for diabetic wound healing. Owing to the dual-dynamic covalent crosslinking network, the CPOA hydrogel showed good injectability and self-healing ability. In addition, the hydrogel displayed reactive oxygen species (ROS) responsive properties, enabling both scavenging of multiple free radicals and on-demand release of Exos in the ROS-rich wound microenvironment. A diabetic wound model was established on C57 mice, and treatment with CPOA@Exos demonstrated that it could promote the polarization of macrophages toward the M2 phenotype, enhance cellular proliferation in the wounded area, and thereby accelerate the healing of diabetic wounds. In conclusion, this study provides a new hydrogel wound dressing that can inhibit inflammation for the management of diabetic wounds.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Rui Hu,Da-Wen Sun,You Tian,Liang Xu,Libin Sun
As a new cryoprotectant, natural deep eutectic solvents (NADESs) have been shown to achieve efficient and safe cryoprotection through excellent thermodynamic characteristics and strong recrystallisation inhibition ability. To further discover the cryoprotection mechanism of NADESs, the transcriptomics of yeast cells cryopreserved by different NADESs were examined. Notably, the PG cryoprotected yeast cells showed the highest viability (Q4: 81.39%) and the earliest regrowth peak (~ 6 h), indicating superior recovery performance. Transcriptomic analysis suggests that NADESs significantly modulate yeast cryotolerance. Differentially expressed genes (DEGs) were predominantly enriched in carbohydrate, amino acid, and fatty acid metabolism. Specifically, key genes involved in glycolysis, such as PCK1 , FBA1 , and ENO1 , were significantly regulated. Furthermore, the up-regulation of osmotic pressure-related genes ( STL1 , FPS1 ) in GF-cryopreserved yeast is consistent with observed osmotic changes. This suggests that proline-containing NADESs may help modulate osmotic pressure, thereby mitigating osmotic damage. Overall, this study supports a more comprehensive understanding of the cryopreservation mechanisms of emerging NADESs. Importantly, while these results support a role for NADES-induced shifts in glycolysis and osmoadaptation, the causality will be further validated in future work using HOG1 Δ, STL1 Δ, and FPS1 Δ strains, as well as pharmacologic MAPK inhibition.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Ruo-Yun Zhang,Wei Yue,Yang Xuan,Long Qi,Lin-Lin Guo,Zhen-Zhen Zhu,Ming Yang,Zhang-Jie Pu,Guo-Qiang Li,Xin-Yi Mao,Ding Zhou,Zhao-Wei Zhang
Immunogenic cell death (ICD) attracts significant attention by initiating adaptive immune responses for antitumor immunotherapy. Although ROS is developed to trigger ICD as an inducer, the effect of immunotherapy is compromised by the scavenging of ROS in the immunosuppressive tumor microenvironment. Herein, we report an injectable cascade-amplified ICD nanofiber hydrogel self-assembled from steroid drug prednisolone sodium phosphate (PSP) and calcium chloride for the local delivery of glutaminase inhibitor CB-839 and the sonosensitizer Ce6 (CCPG). ROS is generated by stimulating Ce6 under ultrasound (US), which disrupts the Ca 2+ buffer capacity of mitochondria to induce Ca 2+ influx, thereby triggering ICD to release damage-associated molecular patterns (DAMPs) from dying tumor cells for initiating T cell infiltration. Moreover, ICD induction is further amplified by the glutaminase inhibitor CB-839. CB-839 suppresses glutathione (GSH) synthesis by inhibiting the conversion of glutamine to glutamate as the precursor of GSH, thereby limiting the scavenging of Ce6-generated ROS and enhancing their antitumor and ICD-triggering effects. Additionally, anti-inflammatory PSP suppresses matrix metalloproteinase-2 (MMP-2) expression, resulting in facilitating T cell infiltration. As a consequence, this work highlights a strategy to reprogram immunosuppressive tumor microenvironment via cascade-amplified ICD by inhibiting the scavenging of ROS to maintain Ca 2+ overload, providing new insights for enhancing the efficacy of immunotherapy.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.7
Gongde Lan,Yuchen Liu,Hongyuan Mao,Han Yu,Weicheng Huang,Pengxiang Chen,Xin Chen,Qingxu Song,Yufeng Cheng,Yuxia Luan
Boron neutron capture therapy (BNCT) is a promising strategy for selective tumor eradication. However, the clinical application of the boron delivery agent p ‑boronophenylalanine (BPA) is limited by challenges such as poor solubility, suboptimal tumor accumulation, and lack of effective imaging capabilities. Here, we present a metal polyphenol-based boron nanodrug (EB@Gd NPs), designed to address these challenges and enhance the efficacy of BNCT. Self-assembled from epigallocatechin-3-gallate (EGCG), BPA, and gadolinium ions (Gd 3+ ), the EB@Gd NPs exhibit significantly improved tumor targeting and enable real-time magnetic resonance imaging (MRI). These nanoparticles show enhanced tumor accumulation, with MRI providing dynamic insights into boron distribution, thus aiding in determining optimal timing for neutron irradiation. Furthermore, EGCG potentiates therapeutic effect of BNCT by inhibiting DNA repair and promoting efficient DNA double-strand breaks. Therefore, the EB@Gd NPs demonstrate potent tumor growth inhibition in BNCT, holding great potential for improving precision and efficacy of BNCT in cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2026
·Vol. 51
·期 1
·50
·
IF 3.8
Zainab Syeda Rida,Khan Jehan Zeb,Batool Syeda Anaa,Nadeem Humaira,Tipu Muhammad Khalid,Fatima Humaira,Ahmad Nadeem,Ul-Haq Zaheer,Irshad Nadeem
1,3,4-Oxadiazole derivatives have attracted substantial attention as promising therapeutic agents for neurodegenerative disorders due to their anti-inflammatory and neuroprotective properties. This study specifically examined a 1,3,4-oxadiazole derivative, i.e.,-{[5-(3-bromophenyl)-1, 3, 4-oxadiazol-2-yl] sulfanyl} ethan-1-ol (abbreviated as OX-1), in the context of multiple sclerosis (MS). Currently, approximately 2.8 million individuals worldwide are living with MS. The study demonstrates the therapeutic potential of OX-1 using the experimental autoimmune encephalomyelitis (EAE) model of MS. Neurobehavioral assessments indicate significant improvements in clinical scoring, motor deficits, muscular strength, and locomotor activity in EAE subjects. Imaging test reveal notable improvements in spinal deformity, further supporting the efficacy of the compound. Comprehensive analyses, including antioxidant assays, RT-PCR, and comet assays, confirm that OX-1 effectively reduces oxidative stress, accompanied by a significant decrease in cytokine expression. Histological examinations reveal critical pathological changes in the hippocampus, cortex, eyes, spinal cord, and optic nerve. The data demonstrate that this compound exhibits neuroprotective effects by activating the Nrf2/HO-1 pathway, thereby reducing oxidative stress and enhancing the antioxidant defense system. Additionally, it suppresses the TLR4/NF-κB pathway, significantly lowering pro-inflammatory cytokine production and immune cell infiltration. Furthermore, molecular docking and simulation studies demonstrate the binding interactions and potential modulatory effects of OX-1 on HO-1 and NF-κB. These results emphasize the therapeutic promise of OX-1 in effectively alleviating the clinical signs and symptoms associated with EAE-induced MS. Graphical The graphical abstract of EAE model of MS.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 3.8
Wang Dong,Li Mengrong,Zhang Xiangyu,Chai Shiwei,Chen Di
A novel ultrasound-assisted magnetic sorbent–wrapped stick dip extraction (UA-MSWS-DE) method was developed for the batch-scale determination of seven tyrosine kinase inhibitors (TKIs) in human plasma. The extraction device was fabricated by immobilizing octadecylphosphonic acid–functionalized magnetic nanoparticles onto flame-sealed glass capillaries embedded with magnets. Ultrasonic-assisted dip extraction was used to extract TKIs from diluted plasma, followed by quantification via liquid chromatography–tandem mass spectrometry (LC-MS/MS). Key extraction parameters were systematically optimized, yielding the following optimal conditions: sample pH 5.0 and volume 1.0 mL, sorbent particle size 20 nm and dosage 10 mg, extraction time 20 min, and desorption with 150 µL of ethanol for 8 min. The method demonstrated excellent linearity ( R 2 ≥ 0.99), low limits of quantification (0.1–5.0 ng/mL), and good intra-/inter-day precision (RSDs < 14.95%). The accuracy in blinded plasma samples, tested at 0.25–25% of the studied concentration range, ranged from 87.13 to 112.33%. Compared to conventional magnetic dispersive solid-phase extraction, the UA-MSWS-DE strategy simplifies handling and is designed to facilitate potential parallel processing using only an ultrasonic bath. Five greenness metrics confirmed the method’s high sustainability. Furthermore, the modular design enables adaptation to other analytes by modifying the sorbent coating, making UA-MSWS-DE a sensitive, green, and automation-ready method for therapeutic drug monitoring. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2026
·
IF 5.9
Mohammad Imtiyaj Khan,Monika Kumari,Hidam Bishworjit Singh,Danni Zhang
Betalainic plants accumulate biosynthetically related betacyanins such as betanidin, betanin (betanidn-5- O -glucoside), and amaranthin [betanidin-5- O -(2′- O -glucuronyl)-glucoside]. In such plants, these pigments are involved in stress tolerance and other physiological roles. These pigments are pharmacoactive and also used as food colorants, smart indicators in packaging films, and others. Herein, we investigated the effects of incremental substitution in their structures using density functional theory (DFT), in vitro antioxidant activity, cytotoxicity, and seedlings of Brassica campestris L., which is a betalain-devoid crop plant. The results showed betanin’s better electron-donating potential, amaranthin’s better in vitro antioxidant activity, and synergistic in vitro cytotoxic effect of all the three above-mentioned betacyanins. Individual betacyanins or in combinations of two of them were not cytotoxic. Betanin (50 mmol/L) produced comparable growth-promoting effect with 24-epibrassinolide (EBR; 10 μmol/L), a brassinosteroid. EBR and betanin did not affect total chlorophylls, but amaranthin enhanced them. Betanin enhanced tyrosine and ascorbic acid in B. campestris seedlings. Amaranthin neither produced a significant growth-promoting effect nor a comparable tyrosine-enhancing effect with betanin. Taken together, our findings establish that betanin is a physiologically more effective compound in planta , particularly in betalain-devoid B. campestris . Considering the importance of tyrosine and ascorbic acid in our diet and betanin’s ability to enhance them in B. campestris , pre-harvest treatment with betanin or betanin-containing formulations may be recommended to enhance the nutritional quality.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121326
·
IF 7.2
Jiaqi Meng,Yun Liu,Debing Wang,Bingbing Zhang,Xianchun Tang,Xinying Zhang,Zhichao Wu,Qiaoying Wang
Advanced oxidation processes utilizing peracetic acid (PAA) have garnered significant interest for the removal of micropollutants, while the mechanisms of employing both the light and thermal energy of solar radiation to activate PAA have not been explored. This study adopted the reduced graphene oxide (rGO) as a photothermal material under solar radiation to synergistically activate PAA for the degradation of carbamazepine (CBZ). The temperature notably increased to a maximum of 63.0 °C under the rGO concentration of 5 mg/L, and an optimal removal efficiency of 92.45 % with a k obs of 0.014 min −1 was achieved. The primary mechanism for CBZ removal was the generation of reactive species through the photoactivation and thermal activation of PAA under solar irradiation. Radical quenching experiments and electron spin resonance analysis identified CH 3 C(O)OO· as the principal contributor to CBZ degradation. Seven potential transformation products of CBZ were identified, and three possible degradation pathways were proposed. The toxicity of CBZ polluted water was effectively reduced after photothermal-activated PAA treatment. The removal efficiency of CBZ remained above 90 % after four consecutive cycles of rGO reuse. This study comprehensively elucidates the mechanism of photothermal activation of PAA and thus contributes to the degradation of micropollutants.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 12.6
Jingya Xiu,Lin Liu,Lingao Yin,Yihong Peng,Ziqi Li,Ziyun Lin,Yilin Song,Degong Yang,Jiulong Zhang,Chunrong Yang
Immunogenic cell death (ICD), instigated by reactive oxygen species (ROS), has emerged as an efficacious strategy for augmenting the immunogenicity of tumor cells. However, the effects of ICD are severely diminished by elevated levels of glutathione (GSH) within tumor cells to maintaining intracellular redox homeostasis. To address this, the novel carrier-free self-assembled nanoparticles are designed to aggravating redox dyshomeostasis by reducing GSH and increasing ROS levels. The nanoparticles (ZDS NPs) were established by the self-assembly of zinc ions (Zn 2+ ), doxorubicin (DOX) and Nrf2 siRNA. The ZDS NPs exhibited the ultra-high entrapment efficiency of DOX (99%) and siRNA (89%), and the releases of DOX and siRNA were both pH-dependent owing to the cleavage of coordinate and hydrogen bonds under acidic conditions. Following the endocytosis of ZDS NPs by tumor cells, redox homeostasis was significantly disrupted, DOX and Zn 2+ enhanced the production of ROS via activating cGAS/STING pathway, whereas siRNA reduced GSH levels by decreasing the expression of Nrf2 protein. This further promoted a stronger ICD effect with elevated secretion of ATP, HMGB1, CRT, thereby inducing the maturation of DCs and activating a more robust anti-tumor immunity. This study presents a novel approach for the synergistic enhancement of ICD in cancer immunotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6
Tongfang Chen,Jingjie Liang,Weiqing Liu,Fengxia Zhou,Chanci Qiu,Yuhui Peng,Xie Zhou
Background Glutathione (GSH), the most abundant cellular non-protein thiol and a central antioxidant, plays essential roles in regulating redox homeostasis, signal transduction, and detoxification processes. Altered GSH levels are closely associated with various pathological conditions, including neurodegenerative disorders, and liver injury . Notably, tumor tissues exhibit markedly elevated GSH concentrations (up to 4-10 times higher than in normal cells), where it promotes tumor proliferation, invasion, and chemoresistance by sustaining a reduced microenvironment, these characteristics make GSH an important biomarker and therapeutic target in oncology. Results We developed a novel "switch-on" near-infrared (NIR) fluorescence nanoprobe, Ag 2 S-CH@Cu-TCPP, for the highly sensitive and selective detection and imaging of GSH. The nanoprobe was constructed by conjugating histidine-rich CH peptides to carboxyl-capped Ag 2 S quantum dots (Ag 2 S QDs), which were then self-assembled onto two-dimensional Cu-TCPP metal-organic framework (MOF) nanosheets via coordination between Cu 2+ and imidazole groups. In this design, Cu-TCPP acts as an efficient quencher for the Ag 2 S-CH fluorescence via a fluorescence resonance energy transfer (FRET) mechanism. Upon encountering GSH, the reduction of Cu 2+ to Cu + disrupts the FRET process, leading to the recovery of the NIR fluorescence signal. This nanoprobe demonstrated a low detection limit of 5.33 μM and a wide linear range from 0.625 to 10 mM for GSH quantification, with excellent selectivity, high reproducibility, and favorable biocompatibility. We successfully applied the platform for monitoring intracellular GSH levels and distinguishing between cancer cells and normal cells based on their differential GSH expression. Significance and Novelty The "switch-on" probe demonstrates excellent biocompatibility and is applicable for cellular imaging. It enables discrimination between normal and cancer cells while supporting semi-quantitative analysis of both endogenous and exogenous GSH. This work provides a novel and reliable strategy for tumor diagnosis and advanced investigations into GSH-related biological processes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）