ELECTROANALYSIS
·
2022
·Vol. 34
·期 8
·1329-1337
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IF 3.077
Jie Liu,Dianping Tang
A simple and novel electrochemical immunoassay based on MXene (Ti 3 C 2 )−Au nanoparticles (AuNPs) was designed for sensitive screening of a disease-related biomarker, prostate-specific antigen (PSA), by using dopamine-loaded liposomes (DLL) for signal amplification. The system involves two parts, namely, sandwich-type immunoreaction to capture DLL and electrochemical measurement of dopamine. The target PSA can cause a specific antigen-antibody reaction and DLL are enriched in the enzyme-labeled pores. After Triton X-100 is injected into the detection cell, the carried DLL was quickly cracked to release dopamine wrapped in the cavity. A nanocomposite consisting of MXene (Ti 3 C 2 ) support to immobilize Au nanoparticles (Ti 3 C 2 −Au) was utilized to modify a glassy carbon electrode, which gives a strongly enhanced differential pulse voltammetric (DPV) signals for dopamine. In this case, the change of DPV signal depends on the amount of dopamine released by liposomes, which is further positively correlated with the concentration of the analyte PSA. Combining the of MXene (Ti 3 C 2 )−AuNPs nanomaterials (large specific surface area, excellent electrical conductivity, and good electrocatalytic properties) with the liposome signal amplification strategy, the electrochemical immunoassay exhibited excellent performance toward PSA determination with a broad linear range of 1 pg/mL to 50 ng/mL and limit of detection down to 0.31 pg/mL (S/N=3) under the optimized testing conditions. High specificity for PSA over other disease-related biomarkers and acceptable nanocomposite/electrode stability were acquired. The excellent analytical performance shows that the current strategy provides an effective detection platform for clinical sample analysis.
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高影响因子
Talanta Open
·
2023
·
IF 0
Yanan Li,Xin Hua,Jiajia Wang,Baokang Jin
In this paper, a novel electrochemical sensing platform for the sensitive detection of interleukin 6 (IL-6) was designed and fabricated. A label-free aptasensor was fabricated by modifying the glassy carbon electrode (GCE) using a nanocomposite composed of carboxylated multi-walled carbon nanotubes (cMWCNTs), cobalt hexacyanoferrate (CoHCF) and gold nanoparticles (AuNPs). The nanocomposite improved electron transfer rate and increased intercalation of signaling molecules. Moreover, it also offers desirable capabilities for the immobilization of biomolecules. The cMWCNTs/CoHCF/AuNPs modified electrode showed good electrochemical characteristics and biocompatibility, thus achieving excellent sensor performance. The differential pulse voltammetry (DPV) was used to detect the peak current of CoHCF in the redox process. The peak current is proportional to the concentration of IL-6 on the electrode surface. Under optimal conditions, the proposed aptamer sensor exhibits a linear range from 0.5 pg/mL to 1000 pg/mL and has a low detection limit of 0.17 pg/mL. In addition, the IL-6 aptamer sensor has good selectivity, high stability, and satisfactory reproducibility, showing broad potential in clinical research and diagnostic applications.
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SENSORS AND ACTUATORS A-PHYSICAL
·
2025
·
IF 4.9
Jun Dong,Xinyue Li,Shaolong Tu,Qingyan Han,Chengyun Zhang,Kaili Ren,Tuo Li,Wei Gao,Jianxia Qi
Surface-enhanced Raman scattering (SERS) was extensively employed in the domains of target analysis and detection owing to its benefits of high sensitivity and nondestructive detection. In this study, polystyrene (PS) microspheres served as templates. PS microspheres were systematically assembled on silicon wafer surfaces using air-liquid interfacial self-assembly. Subsequently, a PMMA-anisole solution was spin-coated to fill the interstices of the microspheres. Following the removal of the templates using cyclohexane ultrasonication method, gold nanoparticles were deposited onto the substrates utilizing the three-phase self-assembly procedure to construct the AuNPs@PMMA sphere-cavity array substrate. When the PMMA-anisole solution concentration was 1%, the spinning speed was 5500 r/min, and the annealing temperature was 120 °C, the most regular nanostructure of the spherical cavity substrate was achieved. The Raman activity of the fabricated substrates was investigated with the selection of Rhodamine (R6G), Crystal Violet (CV) and Aspartame (APM) as target molecules. The experimental results indicated that AuNPs@PMMA sphere-cavity array substrate exhibiting high sensitivity, excellent uniformity, and stability, and the detection limits for R6G and CV were 10 -11 M and 10 -10 M, respectively. In addition, the substrate achieved a low detection limit of 0.0313 g/L for aspartame (APM), demonstrating the potential application of the substrate in food safety testing.
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高影响因子
MEASUREMENT
·
2026
·
IF 6.1
Yi Li,Shunye Huang,Jianyang Zhang,Rongqi Zhang,Feng Xia,Xuqiang Wu,Like Li,Yong Zhao
Cholesterol is a vital biomolecule in the human body. Abnormal blood cholesterol levels may increase the risk of cardiovascular disease and Alzheimer’s disease. Therefore, there is an urgent need to develop a highly sensitive and rapid method of detecting cholesterol concentration in clinical settings. To meet this demand, we developed a reflective fiber-optic cholesterol sensor based on multimode and single-mode fibers (MMF-SMF). The probe uses AuNPs, synthesised using the sodium citrate reduction method, as a sensitising material, and cholesterol oxidase serves as the sensing membrane. This reflective optical fiber surface plasmon resonance (SPR) biosensor enables the detection of cholesterol concentration with high sensitivity. Experimental results demonstrated that Au film- AuNPs coupling enhances the refractive index sensitivity by 41.3 % to 2243.9 nm/RIU. Cholesterol detection sensitivity increases by 16.02 % to 0.210 nm/nM, and the linear detection range expands from 0–50 nM to 0–70 nM and the detection limit decreases to 4.586 nM. The sensor also exhibits excellent selectivity and stability, and can be used for the detection of cholesterol concentration in real serum samples, offering an efficient solution for developing portable biosensors.
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高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2026
·
IF 7
Kun-Yang He,Liang-Liang Cheng,Wan-Lu Zheng,Ya-Nan Zhang
A Fiber-optic surface plasmon resonance (SPR) bilirubin biosensor based on gold nanoparticles (AuNPs) and bilirubin oxidase (BOD) was proposed in this work. Leveraging the refractive index (RI) sensitivity of SPR and the specific binding capability of BOD to bilirubin, this sensor enables specific detection of bilirubin. To improve the sensitivity of bilirubin detection, AuNPs were introduced to amplify the evanescent field for optical signal enhancement while providing a high surface area for increased BOD immobilization sites. Experimental results demonstrated that the sensor achieved a sensitivity of 54.6 nm/mM within the 0–0.14 mM concentration range and a detection limit of $11.5~\mu $ M. This sensor exhibits the advantages of low sample consumption, compact size, rapid response, good stability, and high specificity. Additionally, the accurate detection of bilirubin in real serum samples demonstrates its great application potential for clinical diagnosis.
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高影响因子
ANALYST
·
2026
·
IF 3.6
Yu Sun,Shuo Li,Peng Tang,Yuankai Zhang,Chao Yi,Wang Peng
To address the limitations of traditional pesticide detection methods, including reliance on costly equipment, operational complexity, and time-consuming procedures, an AuNPs-modified Photonic Crystal (AuPC) microfluidic chip is proposed for sensitive and rapid detection of acetamiprid and myclobutanil. A Photonic Crystal (PC) sensor functionalized with AuNPs was developed. Numerical simulations revealed that AuNPs modification significantly enhanced localized electric field intensities, generating high-density electromagnetic hotspots in microsphere gaps, with a maximum field enhancement of 4.8-fold compared to unmodified counterparts. The sensor demonstrated a detection limit of 10−4 g L−1 for both analytes, with linear concentration-dependent spectral red shifts (R2 > 0.96) observed across 1 g L−1 to 10−4 g L−1. Sensitivity enhancement was achieved through synergistic photonic-plasmonic interactions, and a microfluidic-integrated PC platform was constructed, enabling efficient pesticide residue screening.
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PubMed
高影响因子
ACS Omega
·
2018
·
IF 0
Jia Du,Yiming Lu,Jian Zhang,Jinxin Wang,Yanfang Wang,Mao Li,Qidai Chen
Tuning optical limiting was achieved based on the nanostructural and synergistic effects of heterosized gold nanoparticles and fullerene on electrochemical assembly. In particular, with thicknesses of 200, 1, and 10 nm, heterosized AuNP multilayers with periodical pairs of layers present a superior threshold of 0.59 J cm–2 to monosized AuNP films with the values of 0.89–2.55 J cm–2, which was further significantly enhanced by the introduction of C70 with a significant threshold drop from 0.43 to 0.13 J cm–2, indicating that the reverse saturable absorption of C70 had a key contribution compared to the free carrier absorption of AuNPs. This paper not only demonstrates that the hybrid engineering of heterosized AuNPs into an identical film is an effective way to enhance the optical limiting but also indicates that the reverse saturable absorption of C70 is superior to free carrier absorption of AuNPs in optical limiting in AuNPs and C70 hybrid films.
研究领域:
纳米材料
·
光子学
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光学材料
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纳米光子学
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电化学
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Biosensors-Basel
·
2023
·Vol. 13
·期 2
·272
·
IF 5.4
Dong Wang,Xiyu Mao,Yitao Liang,Yu Cai,Tingting Tu,Shanshan Zhang,Tianyu Li,Lu Fang,Yue Zhou,Zhaoyang Wang,Yu Jiang,Xuesong Ye,Bo Liang
Urine analysis is widely used in clinical practice to indicate human heathy status and is important for diagnosing chronic kidney disease (CKD). Ammonium ions (NH4+), urea, and creatinine metabolites are main clinical indicators in urine analysis of CKD patients. In this paper, NH4+selective electrodes were prepared using electropolymerized polyaniline-polystyrene sulfonate (PANI: PSS), and urea- and creatinine-sensing electrodes were prepared by modifying urease and creatinine deiminase, respectively. First, PANI: PSS was modified on the surface of an AuNPs-modified screen-printed electrode, as a NH4+-sensitive film. The experimental results showed that the detection range of the NH4+selective electrode was 0.5~40 mM, and the sensitivity reached 192.6 mA M−1cm−2with good selectivity, consistency, and stability. Based on the NH4+-sensitive film, urease and creatinine deaminase were modified by enzyme immobilization technology to achieve urea and creatinine detection, respectively. Finally, we further integrated NH4+, urea, and creatinine electrodes into a paper-based device and tested real human urine samples. In summary, this multi-parameter urine testing device offers the potential for point-of-care testing of urine and benefits the efficient chronic kidney disease management.Keywords:electrochemical biosensor;PANI: PSS;ammonium ion;urea;creatinine;urinalysis
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PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 40
·期 10
·1141-1153
·
IF 3.815
Guo Xiao-Xiao,Hou Shi-Chang,Chen Jun,Liao Cong,He Wei-Dong
Incautious discharge of organic dyes such as methyl orange (MO) has produced serious pollution to the environment, calling for the efficient techniques to remove them with retaining the green world. The photo-catalytic degradation of organic dyes is promising among the developed techniques. Thus, a strategy based on transpiration-prompted photocatalytic degradation of dye pollutant under sunlight is put forward. Aniline (ANI) is graft-polymerized onto poly(acrylamide- co-N -4-aminophenylacrylamide) (PAAm) cryogel embedded with gold nanoparticles (AuNPs, diameter: 4–10 nm). The obtained cryogels integrated with AuNPs and PANI inside PAAm matrix (AuNP@PAAm- g -PANI) have been structurally explored based on the chemical composition and the phase/porous morphology. SEM and TEM observation shows that PANI and AuNPs are uniformly distributed in PAAm matrix. Since the macro-porosity of cryogel, hydrophilicity of PAAm and photo-thermal activity of PANI, PAAm-g-PANI cryogels without AuNPs can have a photo-thermal evaporation rate of water at 1.63 kg·m −2 ·h −1 . As a comparison, AuNP@PAAm- g -PANI cryogels with AuNPs exhibit higher one at 2.20 kg·m −2 ·h −1 , suggesting the promotion of AuNPs to photo-thermal evaporation. Meanwhile, PANI appreciatively assists AuNPs to display higher catalytic ability for the oxidative degradation of MO. Therefore, the removal of MO from water is obviously prompted by the water transpiration under sunlight with AuNP@PAAm- g -PANI cryogels, whose rate constant can reach to 0.320 h −1 , being three folds of that for the sole absorption of MO. This transpiration-prompted photocatalytic degradation provides a fascinating route to eliminate organic pollutants and obtain pure water from wastewater simultaneously with sustainable sunlight energy.
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高影响因子
Applied Nanoscience
·
2022
·Vol. 12
·期 12
·3883-3899
·
IF 3.869
Farooq Muhammad Umar,Sahin Yesim Muge,Naz Muhammad Yasin,Ijaz Salman,Shukrullah Shazia,Makhlouf Mohamed M.
A paradigm transition in cancer treatment gradually moved from "one drug-one target" toward "multi-target selective drugs design", because different drugs simultaneously inhibit tumor progression differently. The underlying mechanism has encountered significant challenges, such as encapsulating chemotherapies into a nanosized particulate system, adverse side effects, and managing multidrug-resistant. To address these issues, herein we engineered the gold nanoparticles’ surfaces with cross-linked polyethylene glycol that have well-controlled morphology and further encapsulated with commonly known chemo-drugs paclitaxel and bleomycin for nanovectorization of therapeutics to the intended site and there act through the concurrent action of dual targeting mechanisms without creating undesired side effects. The structures of the nanovectorization particulate system were confirmed by various spectroscopic techniques. The efficacy of nanovectorization particulate system and non-targeted free drugs was evaluated using the human cervical adenocarcinoma cell model. Our findings indicate that this nanovectorization particulate system had synergic stimuli-responsive characteristics and unrivaled control of efficient transportation of therapeutics agents. This system meets the high demand for multiple targeting delivery and has substantial benefits across multiple fields of nanomedicine, especially in chemotherapy.
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高影响因子
RSC Advances
·
2021
·Vol. 11
·期 42
·25976-25982
·
IF 3.361
Fengyi Cao,Changmin Wei,Gangqing Ma,Like Hou,Rencong Zhang,Lin Mei,Qi Qin
Cotton gauze has been used as a wound dressing since the 19th century, and still plays an important role in current clinical therapies. However, the antimicrobial ability of cotton gauze is limited. In this work, gold nanoparticles (AuNPs) were used as photothermal transduction agents to synthesize modified photothermal antimicrobial cotton gauze. The modified cotton gauze was synthesized by immersing and heating the clinical cotton gauze with AuNPs solution. XPS, ICP-OES, FTIR, XRD and SEM characterizations confirmed that AuNPs were successfully decorated on the surface of cotton gauzes. Besides, the mechanical properties, air and water vapour permeability performance of cotton gauze were not changed after modification. Photothermal antimicrobial experiments confirmed that AuNPs modified on the cotton gauze could convert light to heat, inducing rapid temperature increase of the cotton gauze. And the heat could kill microbial cells permeated in the modified cotton gauze, giving it the potential of being used for photothermal antimicrobial therapy.
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PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 15
·155603
·
IF 3.874
Jun Dong,Yi Cao,Qingyan Han,Wei Gao,Tuo Li,Jianxia Qi
In this paper, Au nanoparticles (AuNPs) are prepared using wet chemical reduction transfer of dense AuNPs film by self-assembly to the surface of Ag grating, which is inverted from the inner DVD after evaporation. The Ag grating/AuNPs self-assembly hybrid substrate commonly used in surface-enhanced Raman scattering (SERS) research is produced. The coupling effect between AuNP-AuNP and AuNPs-Ag slugs can evidently enhance the local electric field. Experimental results show that the hybrid SERS substrate can detect 10−9 M Rh6G, and the enhancement factor reaches 4.4 × 105. This small, cheap hybrid substrate has enormous potential in the field of SERS sensing.
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高影响因子
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