Journal of Environmental Chemical Engineering
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2023
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IF 7.7
Weichao Xu,Yuxuan Yi,Kunhao Guo,Anming Li,Xuehan Zhang,Feng Guo
The combination of photocatalysis and enzyme catalysis makes many previously difficult or slow chemical reactions easy to realize. A novel [email protected] −C 3 N 4 biohybrid catalyst was successfully synthesized using a simple in situ synthesis method to remove 4- tert -butylphenol (4- t -BP). A series of characterization methods were used to investigate the structure, morphology, chemistry, and optical properties of biohybrid catalysts. [email protected] −C 3 N 4 has improved photogenerated electron-hole pair separation efficiency and charge carrier mobility , as well as photocatalytic activity, with a removal rate of 4- t -BP nearly 100% within 180 min. The catalyst recycling test revealed that the composite could maintain its high removal efficiency after 10 recyclings. Meanwhile, the photocatalytic mechanism investigation reveals that ∙O 2 − play critical roles in the removal of 4- t- BP by g−C 3 N 4 . A detailed examination of the mechanism and pathway of 4- t -BP removal by photoenzyme oxidation was also carried out. This biohybrid catalyst opened up a new avenue for the advancement of environmental protection technology .
研究领域:
环境科学
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化学工程
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材料科学
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催化
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环境技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Yuan-Cheng Zhu,Fei Xu,Nan Zhang,Wei-Wei Zhao,Jing-Juan Xu,Hong-Yuan Chen
This work reports the use of compositionally heterogeneous asymmetric [email protected] core–satellite nanoassembly functionalized with DNA sequence as unique signaling nanoprobes for the realization of new energy-transfer-based photoelectrochemical (PEC) immunoassay of prostate- specific antigen (PSA). Specifically, the [email protected] asymmetric core–satellite nanoassemblies ( [email protected] ACS) were fabricated on a two-dimensional glass substrate by a modified controlled assembly technique, and then functionalized with DNA sequences containing PSA aptamers as signaling nanoprobes. Then, the sandwich complexing between the PSA, its antibodies, and the signaling nanoprobes was performed on a CdS QDs modified indium tin oxide (ITO) electrode. The single stranded DNA can server as a facile mediator that place the [email protected] ACS in proximity of CdS QDs, stimulating the interparticle exciton–plasmon interactions between [email protected] ACS and CdS QDs and thus quenching the excitonic states in the latter. Since the damping effect is closely related to the target concentration, a novel energy-transfer-based PEC bioanalysis could be achieved for the sensitive and specific PSA assay. The developed biosensor displayed a linear range from 1.0×10 −11 g mL −1 to 1.0×10 −7 g mL −1 and the detection limit was experimentally found to be of 0.3×10 –13 g mL −1 . This strategy used the [email protected] ACS-DNA signaling nanoprobes and overcame the deficiency of short operating distance of the energy transfer process for feasible PEC immunoassay. More significantly, it provided a way to couple the plasmonic properties of the Ag NPs and Au NPs in a single PEC bioanalytical system. We expected this work could inspire more interests and further investigations on the advanced engineering of the core–satellite or other judiciously designed nanostructures for new PEC bioanalytical uses with novel properties.
研究领域:
纳米技术
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生物化学
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分析化学
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生物物理学
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材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2019
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IF 4.916
Yi-Kun Li,Wei-tao Li,Xun Liu,Ting Yang,Ming-Li Chen,Jian-Hua Wang
A magnetic functionalized bio-sorbent based on aptamer was designed for the selective separation of ultra-trace Pb 2+ , shortly termed as Fe 3 O 4 @ [email protected] Pb(II) specific aptamer attached to the magnetic solid substrate served as affinity probe to capture and separate trace lead. Oligonucleotides with a polyA block were employed for the immobilization on the surface of AuNPs, with adenine sequences (polyA) as the part of effective anchoring block. The prepared Fe 3 O 4 @ [email protected] composites were characterized by FT-IR, SEM and XPS. The binding of lead on Fe 3 O 4 @ [email protected] composites surface was pH-dependent, the adsorption follows Langmuir model, and the adsorption dynamic fits the pseudo-second-order kinetics model. Procedure for lead separation and preconcentration was explored and combined with detection of graphite furnace atomic absorption spectrometry (GFAAS). Under the optimum condition, an enrichment factor of 17.73 was obtained with a sample volume of 1.0 mL. The limit of detection (LOD) was 57 ng L −1 along with a relative standard deviation (RSD) of 2.06 (n = 9). The procedure was further validated by a certified reference material GBW08608 and several environmental and blood samples.
研究领域:
环境科学
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分析化学
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材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
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2018
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IF 8.806
Zuyuan Luo,Siqi Zhang,Jijia Pan,Rui Shi,Hao Liu,Yalin Lyu,Xiao Han,Yan Li,Yue Yang,Zhixiu Xu,Yi Sui,En Luo,Yuanyuan Zhang,Shicheng Wei
The efficacy of stem cell-based bone tissue engineering has been hampered by cell death and limited fate control. A smart cell culture system with the capability of sequentially delivering multiple factors in specific growth stages, like the mechanism of the natural extracellular matrix modulating tissue formation, is attractive for enhancing cell activity and controlling cell fate. Here, a bone forming peptide-1 (BFP-1)-laden mesoporous silica nanoparticles ( [email protected] ) incorporated adhesion peptide, containing the arginine-glycine-aspartic acid (RGD) domain, modified alginate hydrogel (RA) system ( [email protected] ) was developed to promote the activity and stimulate osteo-differentiation of human mesenchymal stem cells (hMSCs) in sequence. The survivability and proliferation of hMSCs were enhanced in the adhesion peptide modified hydrogel. Next, BFP-1 released from [email protected] induced hMSCs osteo-differentiation after the proliferation stage. Moreover, BFP-1 near the cells was self-captured by the additional cell-peptide cross-linked networks formed by the ligands (RGD) binding to receptors on the cell surface, leading to long-term sustained osteo-stimulation of hMSCs. The results suggest that independent and sequential stimulation in proliferation and osteo-differentiation stages could synergistically enhance the survivability, expansion, and osteogenesis of hMSCs, as compared to stimulating alone or simultaneously. Overall, this study provided a new and valid strategy for stem cell expansion and osteo-differentiation in 2D or 3D culture systems, possessing potential applications in 3D bio-printing and tissue regeneration .
研究领域:
组织工程
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干细胞生物学
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生物医用材料
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再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）