PATHOLOGY RESEARCH AND PRACTICE
·
2025
·
IF 3.2
Ting-Ting Yang,Xin-Xin Ma,Xuan Zhang,Zhen-Qing Han,Shuang Zhang,Qi Wang,Hong-Wei Dong,Jia-Ren Liu
β-Ionone (BI) has shown the potent inhibitory malignant progression of multiple tumors including gastric cancer in vitro and in vivo. However, the mechanism by which BI inhibits gastric cancer remains to be clarified, especially in ferroptosis, which is a new form of programmed cell death. The aim of this study was to investigate whether BI could induce ferroptosis in gastric cancer and the underlying mechanisms. The results manifested that BI obviously inhibited cell viability and increased the Fe 2 + level, total reactive oxygen species (ROS) and lipid ROS levels and the malondialdehyde (MDA) concentrations, but reduced the mitochondrial membrane potential (MMP) and the expression of SLC7A11 and GPX4 in MKN45 cells and AGS cells ( P < 0.05 or P < 0.01). Besides, BI also arrested cell cycle and decreased the expression of Cyclin D1 and CDK4 ( P < 0.05 or P < 0.01). Mechanistically, BI reduced the levels of β-catenin and pGSK-3β/GSK-3β in MKN45 cells and AGS cells ( P < 0.05 or P < 0.01). Nevertheless, pretreatment with lithium chloride (LiCl), an inhibitor of GSK-3β, reversed BI-induced ferroptosis and cell cycle arrested in MKN45 cells ( P < 0.05). In addition, BI effectively inhibited the growth of gastric cancer xenografts and downregulated the levels of GPX4, SLC7A11, PCNA, β-catenin and pGSK-3β/GSK-3β ( P < 0.05 or P < 0.01). Collectively, our study reveals that BI inhibits proliferation of gastric cancer cells by induction ferroptosis via the Wnt/β-catenin signaling pathway and provides a novel therapy for gastric cancer.
研究领域:
肿瘤学
·
细胞生物学
·
药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2024
·
IF 5.1
Lei Zhong,Qiuhui Hu,Qiping Zhan,Mingwen Zhao,Liyan Zhao
Individuals with immune disorders cannot establish an adequate defense to pathogens, leading to gut microbiota dysbiosis. β-Carotene can regulate immune response, but its bioavailability in vivo is very low. Herein, we developed a glycosylated oat protein-based nanoparticle to improve the application of β-carotene for mitigating cyclophosphamide-induced immunosuppression and gut microbiota imbalance in mice. The results showed that the nanoparticles facilitated a conversion of β-carotene to retinol or retinyl palmitate into the systemic circulation, leading to an increased bioavailability of β-carotene. The encapsulated β-carotene bolstered humoral immunity by elevating immunoglobulin levels, augmenting splenic T lymphocyte subpopulations, and increasing splenic cytokine concentrations in immunosuppressed mice. This effect was accompanied by the alleviation of pathological features observed in the spleen. In addition, the encapsulated β-carotene restored the abnormal gut microbiota associated with immunosuppression, including Erysipelotrichaceae, Akkermansia, Bifidobacterium and Roseburia. This study suggested that nanoparticles loaded with β-carotene have great potential for therapeutic intervention in human immune disorders by specifically targeting the gut microbiota.
研究领域:
食品科学
·
免疫学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-11
·
IF 4.011
Geng Qianqian,Li Tian,Wang Xin,Chu Weijing,Cai Mengling,Xie Jingchan,Ni Hanwen
In this work, the inclusion complexes of hydrophobic herbicide bensulfuron-methyl (BSM) with β-cyclodextrin (β-CD) and (2-hydroxypropyl)-β-CD (2-HP-β-CD) were prepared and characterized. Phase solubility study showed that both β-CD and 2-HP-β-CD increased the solubility of BSM. Three-dimensional structures of the inclusion complexes were simulated by the molecular docking method. The docking results indicated that guest BSM could enter into the cavities of host CDs, folded, and centrally aligned inside the inclusion complexes. The benzene ring of the guest molecule was close to the wide rim of the host molecules; the pyrimidine ring and side chains of the guest molecule were oriented toward the narrow rim of the host molecule. The inclusion complexes were successfully prepared by the coprecipitation method. The physiochemical characterization data of 1 H NMR, FT-IR, XRD, and DSC showed that the guest and host molecules were well included. BSM had lower soil adsorption and higher herbicidal activity in the complexation form with β-CD or 2-HP-β-CD than in the pure form. The present study provides an approach to develop a novel CDs-based formulation for hydrophobic herbicides.
研究领域:
农业科学
·
环境科学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Surui Lu,Hong Deng,Chenyao Zhou,Zhengda Du,Xuena Guo,Yanfei Cheng,Xiuping He
β-Caryophyllene is a plant-derived bicyclic sesquiterpene with multiple biological functions. β-Caryophyllene production by engineered Saccharomyces cerevisiae represents a promising technological route. However, the low catalytic activity of β-caryophyllene synthase (CPS) is one of the main restrictive factors for β-caryophyllene production. Here, directed evolution of the Artemisia annua CPS was performed, and variants of CPS enhancing the β-caryophyllene biosynthesis in S. cerevisiae were obtained, in which an E353D mutant enzyme presented large improvements in Vmax and Kcat. The Kcat/Km of the E353D mutant was 35.5% higher than that of wild-type CPS. Moreover, the E353D variant exhibited higher catalytic activity in much wider pH and temperature ranges. Thus, both the higher catalytic activity and the robustness of the E353D variant contribute to the 73.3% increase in β-caryophyllene production. Furthermore, the S. cerevisiae chassis was engineered by overexpressing genes related to β-alanine metabolism and MVA pathway to enhance the synthesis of the precursor, and ATP-binding cassette transporter gene variant STE6T1025N to improve the transmembrane transport of β-caryophyllene. The combined engineering of CPS and chassis resulted in 70.45 mg/L of β-caryophyllene after 48 h of cultivation in a test tube, which was 2.93-fold of that of the original strain. Finally, a β-caryophyllene yield of 594.05 mg/L was obtained by fed-batch fermentation, indicating the potential of β-caryophyllene production by yeast.
研究领域:
生物技术
·
代谢工程
·
合成生物学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2018
·
IF 3.129
Jianfei He,Fengxian Guo,Luan Lin,Hongbin Chen,Jie Chen,Yongqiang Cheng,Zong-Ping Zheng
The inclusion complexes of oxyresveratrol (Oxy) with β-cyclodextrin (β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) were prepared and characterized by UV–vis spectroscopy, FT-IR spectroscopy, XRD , TG-DTG and SEM . Based on the optimal formulation, 222.23 ± 1.52 μg and 232.65 ± 2.64 μg of Oxy was included in 1.00 mg of the Oxy-β-CD and Oxy-HP-β-CD dried powder with the water solubility up to 14.44 ± 0.83 mg/mL and 47.33 ± 0.78 mg/mL, yielding about 30 and 100-fold increase in Oxy solubility (about 0.47 mg/mL), respectively. The physical stability and dissolution rate study showed that the formation of inclusion complexes significant enhanced the storage stability of Oxy and its dissolution rate. These results indicated that β-CD and HP-β-CD may be suitable delivery systems of Oxy. Furthermore, as illustrated in this study, inclusion complexes exhibited strong antibrowning effects on fresh grape juice in 24 h, showing its great potential application in the food industry.
研究领域:
食品科学
·
药物化学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hongxia Wang,Ludan Hu,Lin Peng,Jie Du,Miaochuan Lan,Yang Cheng,Liang Ma,Yuhao Zhang
Dual encapsulation of β-carotene (CAT) by β-cyclodextrin (CCLD) and chitosan (CS) are prepared via self-assembly process by special addition order and concentration. CCLD and CS alone could not effectively stabilize CAT, while CAT could be encapsulated in cavity of CCLD and the inclusion complex could be further strengthened by CS, due to hydrogen-bonding between CCLD and CS via groups including NH 2 and OH. The dispersion system based on dual encapsulation of CAT had outstanding shear-thinning behavior, proper pseudoplastic properties, satisfactory yield stress, excellent thermal stability and great thixotropy, illustrating high potential for 3D printing. 3D printing of CAT-encapsulated system with high-content CS on paper and bread proves its excellent extrudability and printability, with possible potential in nutrition personalization. The designed host encapsulation structure by CCLD and CS plays a guiding role in incorporating functional materials including bioactives, probiotics, enzymes, vitamins, etc., and provides a reference in innovative food technology.
研究领域:
食品科学
·
材料科学
·
化学工程
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHIRALITY
·
2020
·Vol. 32
·期 7
·1008-1019
·
IF 2.171
Yingjie Li,Xiaotong Lin,Shili Qin,Lidi Gao,Yimin Tang,Shuren Liu,Yuanyuan Wang
Covalent organic frameworks (COFs) as a novel stationary phase have attracted much attention in the field of chromatography owing to their permanent nanoscale porosity, higher surface area, and exceptional stabilities. Here, a novel isocyanate-β-cyclodextrin-modified COF (MDI-β-CD-modified COF) was synthesized using isocyanate-β-cyclodextrin as the chiral selector and imine-based TpPa-1 COF as the matrix by a bottom-up strategy. The reaction condition and the structure of MDI-β-CD-modified COF were optimized and characterized by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectra, nitrogen adsorption/desorption (Brunauer–Emmett–Teller [BET]), and thermogravimetric analysis (TGA). And then the coated open-tubular column (OT column) was prepared using MDI-β-CD-modified COF as chiral stationary phase (CSP) by in situ growth approach, which exhibited excellent stability and repeatability. For seven consecutive runs, the intraday and interday relative standard deviations (RSDs) were in range from 0.35% to 2.21% for the migration time of histidine. The column-to-column reproducibility ranged from 2.39% to 3.08%. Meanwhile, the separation of eight compounds including four amino acids and four β-blockers by capillary electrochromatography sufficiently verified the favorable chiral resolution properties of the MDI-β-CD-modified COF-coated OT column. This strategy of fabricating MDI-β-CD-modified COF-coated OT column expanded the application of imine-based COFs in chromatographic analytical fields.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hailin Wang,Leiwen Xiang,Zhuangwei Zhang,Guoqiang Li,Peng Li,Wentao Chen,Yu Fang,Xinyan Lin,Shufen Lin
Polyphenol-protein interactions are crucial for food processing, nutrition, and functional properties. This study investigates the interaction between rutin and β-casein (β-CAS) or β-lactoglobulin (β-LG) using multispectral analysis, molecular docking, and molecular dynamics (MD) simulations. Fluorescence spectroscopy reveals that rutin binds spontaneously (ΔG < 0) to β-CAS and β-LG, forming complexes with binding constants (Ka) at 298 K of 42.500 × 10 3 and 2.101 × 10 3 L·mol −1 , respectively, and at 308 K of 5.814 × 10 3 and 4.350 × 10 3 L·mol −1 . Multispectral analysis and microscopy reveal complex formation and changes in the proteins' secondary, crystalline, and microstructures. Molecular docking and MD simulations verify complex stability, showing heightened binding affinity between rutin and β-CAS. These results validate hydrophobic interactions and hydrogen bonding as the main forces between rutin and the two proteins. These findings offer insights for using milk proteins as rutin carriers and support potential food industry application.
研究领域:
食品科学
·
生物化学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Junping Zeng,Haitao Huang,Shiyuan Liu,Haibo Xu,Jin Huang,Jiahui Yu
This research is aimed to develop a kind of hollow nanosphere based on β-cyclodextrin-grafted α, β-poly(aspartic acid) (β-CD- graft -PAsp) as drug carrier to enhance the stability of camptothecin (CPT) in aqueous media. Firstly, mono(6-(2-aminoethyl) amino-6-deoxy)-β-cyclodextrin (β-CDen) was synthesized by a substitution reaction between mono-6-deoxy-6-(p-tolylsulfonyl)-β-cyclodextrin (6-OTs-β-CD) and ethylenediamine; and then, the five-member rings in poly( l -succinimide) (PSI) were successively opened by β-CDen to obtain β-CD- graft -PAsp. The synthesized β-CD- graft -PAsp was found to form the unique hollow nanosphere with the internal hole of about 17 nm and many β-CD cavities of 0.7 nm interspersed on the shell. The drug-loading behavior of the hollow nanosphere was also evaluated by using CPT as guest molecule of β-CD. It was worth of note that the β-CD- graft -PAsp hollow nanosphere as carrier enhanced the stability of CPT in aqueous media, and the CPT from the β-CD- graft -PAsp hollow nanosphere displayed a sustained release profile and hence resulted in the essential decrease of cytotoxicity to L929 cell line. Furthermore, almost no cytotoxicity of the β-CD- graft -PAsp is desirable for the application of drug carrier. As a result, the β-CD- graft -PAsp hollow nanosphere showed a great potential as the carrier of CPT.
研究领域:
药学
·
纳米技术
·
材料科学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
·
2021
·Vol. 53
·期 14
·2435-2448
·
IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
·
生物化学
·
疫苗研究
✓ 已验证
DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Xinming Feng,Jing Guo,Rubing Zhang,Wei Liu,Yujin Cao,Mo Xian,Huizhou Liu
An aminotransferase ω-TAEn was identified from Enhydrobacter aerosaccus. The ω-TAEn was successfully expressed in Escherichia coli and the obtained enzyme showed activity toward β-phenylalanine (β-phe) at optimal conditions. For optically pure (R)-β-phe, 50% yield was observed by kinetic resolution of racemic amino with pyruvate as the amino acceptor. To obtain (S)-β-phe, the lipase/ω-TAEn catalytic system was adopted. The ω-TAEn showed strict stereoselectivity to the amino donor. The formation of (S)-β-phe was observed using 3-aminobutyric acid as the amino donor, and (S)-β-phe was obtained by asymmetric synthesis with a yield of 82%.
研究领域:
生物化学
·
生物催化
·
酶学
·
化学生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of the Australian Ceramic Society
·
2018
·Vol. 55
·期 1
·71-76
·
IF 0.587
Du Xiaoni,Liu Yang,Wang Xiaohong,Feng Jingqing,Ren Chaofei
α-Bi 2 O 3 and β-Bi 2 O 3 nanoparticles were selectively synthesized via one-step solution combustion synthesis (SCS) using bismuth nitrate (Bi(NO 3 ) 3 ·5H 2 O) as the oxidant and tartaric acid (C 4 H 6 O 6 ) as the fuel, and α/β-Bi 2 O 3 heterojunction was rapidly prepared by treating SCS β-Bi 2 O 3 powders with NaOH solution. The physical and chemical properties of as-prepared samples were characterized by XRD, SEM, TEM, PL, and UV-vis techniques. The formation of α/β-Bi 2 O 3 heterojunction can be proven by TEM, UV-vis diffuse reflectance spectra, and PL spectra results. The band gap energies of α-Bi 2 O 3 , β-Bi 2 O 3 , and α/β-Bi 2 O 3 measured by UV-vis diffuse reflectance spectra were estimated to be about 2.9, 2.48, and 2.7 eV, respectively. The PL spectra of α/β-Bi 2 O 3 heterojunction showed higher efficiency of charge separation and transfer across the α–β phase junction than those of α-Bi 2 O 3 and β-Bi 2 O 3 , leading to the enhancement of photocatalytic activity. The synthesized α/β-Bi 2 O 3 heterojunction can degrade 86% of rhodamin B (RhB) after 180 min under visible-light irradiation, better than those of pure α-Bi 2 O 3 (24%, 180 min) and pure β-Bi 2 O 3 (60%, 180 min). Consequently, α/β-Bi 2 O 3 heterojunction is a promising photocatalyst, which can be easily prepared by SCS and post-treatment of NaOH solution.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）