COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Chengwei Wu,Xiyue Duan,Xuzhao He,Wenjian Weng,Hang Zhou,Gaorong Han,Zuobing Chen,Wen Chen,Kui Cheng
Neuroinflammation associated with inflammatory activation of astrocytes on the interface of implanted neural electrodes is the main cause of electrode failure. In this work, collagen/polypyrrole composite film (CPF) was designed and fabricated to modify the interface. The CPF had the co-exposure of collagen and polypyrrole in morphology, which allowed the biomimetic microenvironment provided by collagen to effectively inhibit the inflammatory activation of astrocytes. Meanwhile, the polypyrrole in the composite film significantly improved the electrochemical performance of the electrode interface, enabling it to provide an electrical microenvironment that inhibited inflammatory activation of astrocytes under electrical stimulation. Moreover, mechanistic analysis showed that appropriate electrical stimulation could upregulate calcium ion concentration while inhibit the activation of SOCE and inflammation-related signaling pathways. However, over-voltage stimulation increased ROS levels and reactivated pro-inflammatory signaling pathways. In general, benefiting from having both collagen biochemical cues and the ability to regulate the electrical microenvironment, CPF enabled astrocytes to have the lowest inflammatory factor secretion and inflammatory marker expression under appropriate electrical stimulation, effectively inhibiting the exacerbation of neuroinflammation. This work provides a new perspective on anti-neuroinflammation of the electrode interfaces.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Ruimin Ran,Xiaoyun Luo,Dongmei Zhai,Yeqing He,Xin Guo,Linjing He,Guoying Li
Accurate temperature control during food transportation is essential, as temperature fluctuations can accelerate bacterial proliferation and fat rancidity. Optimal packaging strategies should, therefore, minimize temperature variability in stored food whilst proactively inhibiting microbial growth, delaying fat rancidity, and enabling real-time freshness monitoring. In this study, a multifunctional bilayer collagen film (COL-OPCD) was prepared by a layer-by-layer deposition technique, consisting of an inner layer with antimicrobial, antioxidant, and pH-sensitive properties (oxidized bilberry extract/Pickering emulsion/collagen) and an outer thermally insulating layer (citric acid-diatomite, CD/collagen). The COL-OPCD film exhibited excellent thermal insulation (thermal diffusivity, 0.131 mm 2 /s; reflectivity, 64.71%), antioxidant activity (DPPH radical scavenging, 91.83%; ABTS radical scavenging, 99.73%), and antibacterial activity ( Escherichia coli inhibition rate, 90.07%; Staphylococcus aureus inhibition rate, 87.09%), and allowed real-time monitoring of freshness through visible color changes. It should be emphasized that due to the favorable protection provided by the insulated outer layer, temperature fluctuations inside the package were minimized, with a maximum temperature of 4.80 °C lower than that of the control group, preserving the antioxidant, antimicrobial, and pH-responsive properties of the inner film under high-temperature conditions. An assessment of shrimp preservation at 37 °C showed that the COL-OPCD film not only retarded temperature fluctuations in the packaging shrimp but also extended the shrimp shelf life (9 h) and monitored shrimp freshness in real-time.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yi Li,Tian Tu,Shasha Zhou,Aixia Jiang,Jinglei Wu
Hydrogels with anti-inflammatory and antibacterial capacity have gained increasing attention in regenerative medicine. Dimethyl itaconate (DMI), a derivative of itaconic acid, exhibits powerful anti-inflammatory activity. Herein, we report a green synthesis of a chitosan-dimethyl itaconate (CS-DMI) conjugate through a nucleophilic substitution reaction, where primary amines of chitosan attack the ester carbonyl groups of DMI to form conjugation under mild alkaline conditions. The resulting conjugate was subsequently crosslinked with genipin into a hydrogel. Our findings indicate that this reaction fundamentally changed the nature of CS, converting it from acidic aqueous solution into a nearly neutral aqueous CS-DMI solution. It improved the efficiency of genipin crosslinking while maintaining the robust antibacterial activity of CS. The CS-DMI hydrogel showed sustained drug release of DMI and greater compression strength over the CS hydrogel. The CS-DMI conjugate showed good cytocompatibility, significantly promoted macrophage polarization toward an M2-like phenotype, reducing reactive oxygen species and nitric oxide production in lipopolysaccharide-stimulated macrophages. In a murine subcutaneous implantation model, the CS-DMI hydrogel significantly reduced immune cell infiltration, primarily by suppressing neutrophil recruitment and promoting a shift in macrophage polarization toward the pro-healing M2 phenotype. These findings confirm a viable strategy for developing multifunctional hydrogels that simultaneously address anti-inflammatory and antibacterial needs for modulating inflammation in regenerative medicine.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Yiying Wei,Bo Cong,Xiaopu Ji,Dangyang Wang,Jinghui Hu,Zonglei Mou,Haiqing Song,Yue Zhi,Yushu Liu,Guofeng Zhang,Wan Ru Sun,Pingyu Wang,Deqiang Wang,Shuyang Xie,Ranran Wang
Flexible wearable sensors have garnered significant attention in recent years and exhibit promising application potential in the field of healthcare. Conductive hydrogels have emerged as ideal materials for the development of flexible wearable sensors due to their tunable structural properties, mechanical flexibility, and ease of processing. Nevertheless, most hydrogels are primarily engineered for detecting physiological signals, such as movement and electromyography (EMG), yet lack therapeutic functionalities. In addition, Previous reported hydrogels fabricated from synthetic polymers may retain residual small-molecule monomers, posing cytotoxicity risks. Herein, we report a multifunctional conductive hydrogel composed of carboxymethyl cellulose (CMC), polydopamine (PDA), and polypyrrole-polydopamine-MnO x nanoparticles (P-NPs), designed for concurrent motion monitoring, electromyography (EMG) signal detection, and photothermal therapy. The hydrogel was synthesized through a one-step amidation/oxidative polymerization approach, yielding an interpenetrating dual-network architecture with enhanced mechanical strength and tissue adhesion relative to single-network systems. The incorporation of P-NPs imparts high electrical conductivity, enabling real-time monitoring of physiological signals (e.g., joint movement, muscle activity), as well as effective photothermal conversion under near-infrared (NIR) irradiation. Notably, the hydrogel facilitates on-demand photothermal therapy to mitigate muscle fatigue upon detection of abnormal EMG signals, offering a strategy to prevent exercise-induced injury. Owing to its excellent biocompatibility and stability, the CMC/PDA/P-NPs hydrogel represents a promising platform for next-generation wearable devices integrating diagnostic and therapeutic functions.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Sentao Hu,Zi Ye,Ying Xu,Liaobing Xin,Xiangyou Zheng,Songying Zhang,Lie Ma
Intrauterine adhesions (IUAs), a leading cause of uterine infertility, present significant therapeutic challenges, with conventional treatments often yielding unsatisfactory reproductive outcomes. Although mesenchymal stem cell (MSC)-based therapies have demonstrated considerable promise, current biomaterial delivery systems face critical limitations, particularly in addressing the oxidative stress and inflammatory microenvironment of the damaged endometrium that impairs stem cell viability and function. Herein, we developed an injectable antioxidant hydrogel incorporating microspheres loaded with umbilical cord-derived MSCs (TPG hydrogel) for endometrial regeneration and fertility restoration. The TPG hydrogel was formed via dynamic phenylboronic ester crosslinking, endowing it with shear-thinning injectability for minimally invasive administration and potent reactive oxygen species (ROS) scavenging capacity to mitigate oxidative stress. This antioxidant microenvironment protected encapsulated MSCs from oxidative damage, thereby enhancing their therapeutic efficacy. Furthermore, the incorporation of EPLQLKM (E7) peptide-modified gelatin microspheres provided a bioactive niche that promoted cell adhesion and proliferation. Comprehensive in vitro and in vivo studies demonstrated that the TPG hydrogel exhibited dual functionality, including effective ROS scavenging and immunomodulatory effects characterized by the promotion of macrophage polarization toward the anti-inflammatory M2 phenotype. In a rat model of IUAs, the TPG hydrogel significantly enhanced endometrial regeneration and restored fertility. These findings highlight the TPG hydrogel as a promising therapeutic platform for the treatment of endometrial injury and fertility impairment associated with IUAs.
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Bioactive Materials
·
2022
·
IF 16.874
Tianshu Chu,Qingye Li,Chun Dai,Xiang Li,Xiang Kong,Yangming Fan,Hongyan Yin,Jianjun Ge
Vein grafts are widely used for coronary artery bypass grafting and hemodialysis access, but restenosis remains the "Achilles' heel" of these treatments. An extravascular stent is one wrapped around the vein graft and provides mechanical strength; it can buffer high arterial pressure and secondary vascular dilation of the vein to prevent restenosis. In this study, we developed a novel Nanocellulose-gelatin hydrogel, loaded with the drug Astragaloside IV (AS-IV) as an extravascular scaffold to investigate its ability to reduce restenosis. We found that the excellent physical and chemical properties of the drug AS-IV loaded Nanocellulose-gelatin hydrogel external stent limit graft vein expansion and make the stent biocompatible. We also found it can prevent restenosis by resisting endothelial-to-mesenchymal transition (EndMT) in vitro . It does so by activating autophagy, and AS-IV can enhance this effect both in vivo and in vitro . This study has added to existing research on the mechanism of extravascular stents in preventing restenosis of grafted veins. Furthermore, we have developed a novel extravascular stent for the prevention and treatment of restenosis. This will help optimize the clinical treatment plan of external stents and improve the prognosis in patients with vein grafts.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Rong Rong,Hao Li,Xiangyang Dong,Liqun Hu,Xiaowen Shi,Yumin Du,Hongbing Deng,Yue Sa
Proper bone scaffolds should be biocompatible, mechanically robust and porous for cell migration. Here, pure silk fibroin (SF)- chitosan (CS) aerogel scaffolds reinforced with different amount of SF nanofibers (SF-CS/NF 1% , SF-CS/NF 2% and SF-CS/NF 3% ) are prepared for bone regeneration. Surface morphology and composition were analyzed to ensure successful integration of each component. Incorporating 3 % nanofibers endowed the aerogels with a resistance to 3.5 times the compressive stress of the pure SF-CS aerogels. The benefits of nanofibers were also confirmed by the high porosity of 72.3 ± 1.3 %, the regulated pore size and the high-water uptake ratio of 1770.4 ± 156.8 %. Enhanced cell viability of the aerogel scaffolds was verified with Cell Counting Kit-8 (CCK-8) assays, and confocal microscopy and scanning electron microscopy (SEM) images were taken to assess the cell migration and distribution. The cell differentiation on the aerogel scaffolds was evaluated with enzyme-linked immunosorbent assay (ELISA). Significantly higher level of Collagen type I (Col-I), osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP) expression was observed on SF-CS/NF 3% aerogels. This biocompatible nanofiber-reinforced aerogel scaffold facilitates osteogenic differentiation by rougher surface, enhanced mechanical strength and well-regulated pores. Thus, as-prepared scaffolds may be further applied in bone regeneration field.
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MOLECULES
·
2022
·Vol. 27
·期 9
·2906
·
IF 4.927
Shuying Han,Zhaohua Zhang,Jia Chen,Jie Li,Mi Zhou,Zejian He,Zhen He,Longfei Li
Tympanic membrane perforation (TMP), a common disease, often needs a scaffold as the patch to support surgery. Due to the environment of auditory meatus, the patch can be infected by bacteria that results in failure; therefore, the ideal scaffold may combine biomimetic and antibacterial features. In this work, gelatin was used as the electrospinning framework, genipin as the crosslinking agent, and levofloxacin as an antibacterial in order to prepare the scaffold for TMP. Different contents of levofloxacin have been added to gelatin/genipin. It was found that, with the addition of levofloxacin, the gelatin/genipin membranes exhibit improved hydrophilia and enhanced tensile strength. The antibacterial and cell-cultured experiments showed that the prepared antibacterial membranes had excellent antibacterial properties and good biocompatibility, respectively. In summary, levofloxacin is a good group for the gelatin/genipin scaffold because it improves the physical properties and antibacterial action. Compared with different amounts of levofloxacin, a gelatin/genipin membrane with 1% levofloxacin is more suitable for a TM.Keywords:gelatin;genipin;levofloxacin;nanofiber membrane;tympanic membrane
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Jinghui Jiang,Xiaoxia Chen,Gong-Liang Zhang,Hongshun Hao,Hong-Man Hou,Jingran Bi
To improve the mechanical and barrier properties of cellulose and chitosan (CS) and to allow the application of volatile antibacterial benzyl isothiocyanate (BITC) in active packaging, a double-layer nanocomposite film was prepared. Cellulose nanofibers (CNF) were crosslinked with CS via genipin to build the substrate. Quaternized cellulose nanocrystals (QCNC) were synthesized for carrying BITC as the coating material . By the layer-by-layer self-assembly approach, CS-CNF/QCNC-BITC film was fabricated. This film possessed the tensile strength of 33.75 MPa, low permeabilities of oxygen (6.9 × 10 −17 m 3 /s·m·Pa) and moisture (1.2 × 10 −11 g/s·m·Pa), and good antibacterial activity with the inhibition zone diameters of 4.9, 4.2 and 2.7 cm against Escherichia coli , Salmonella typhimurium and Staphylococcus aureus . The total viable count , total volatile basic nitrogen and thiobarbituric acid-reactive substances of the chicken wrapped CS-CNF/QCNC-BITC were only 4.4 log CFU/g, 17.7 mg/100 g and 0.44 mg/kg at 14 days, indicating a potential application of CS-CNF/QCNC-BITC for food packaging.
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Biomimetics
·
2022
·Vol. 7
·期 2
·41
·
IF 3.743
Qing Min,Danlei Tian,Yuchen Zhang,Congcong Wang,Ying Wan,Jiliang Wu
An emulsification method was developed for fabricating core-shell microspheres with a thick shell layer. Kartogenin (KGN) and platelet-derived growth factor BB (PDGF-BB) were respectively loaded into the core portion and the shell layer of the microspheres with high loading efficiency. The optimally built microspheres were combined with chitosan (CH) and silk fibroin (SF) to construct a new type of composite hydrogel with enhanced strength and elasticity, using genipin or/and tyrosinase as crosslinkers for the intended use in cartilage tissue engineering. The composite hydrogels were found to be thermo-responsive at physiological temperature and pH with well-defined injectability. Rheological measurements revealed that they had an elastic modulus higher than 6 kPa with a high ratio of elastic modulus to viscous modulus, indicative of their mechanically strong features. Compressive measurements demonstrated that they possessed well-defined elasticity. In addition, some gels had the ability to administer the temporal separation release of PDGF-BB and KGN in an approximately linear manner for several weeks. The released PDGF-BB was found to be bioactive based on its effects on Balb/c 3T3 cells. The composite gels supported the growth of seeded chondrocytes while preserving their phenotype. The results suggest that these composite gels have the potential for endogenous cartilage repair.Keywords:composite hydrogel;core-shell microspheres;strength and elasticity;kartogenin;platelet-derived growth factor BB;cartilage tissue engineering
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Bioactive Materials
·
2022
·
IF 16.874
Zijian Shao,Tianyu Yin,Jinbo Jiang,Yang He,Tao Xiang,Shaobing Zhou
Neovascularization is critical to improve the diabetic microenvironment, deliver abundant nutrients to the wound and promote wound closure. However, the excess of oxidative stress impedes the healing process. Herein, a self-adaptive multifunctional hydrogel with self-healing property and injectability is fabricated through a boronic ester-based reaction between the phenylboronic acid groups of the 3-carboxyl-4-fluorophenylboronic acid -grafted quaternized chitosan and the hydroxyl groups of the polyvinyl alcohol, in which pro-angiogenic drug of desferrioxamine (DFO) is loaded in the form of gelatin microspheres ( [email protected] ). The boronic ester bonds of the hydrogel can self-adaptively react with hyperglycemic and hydrogen peroxide to alleviate oxidative stress and release [email protected] in the early phase of wound healing. A sustained release of DFO is then realized by responding to overexpressed matrix metalloproteinases. In a full-thickness diabetic wound model, the [email protected] loaded hydrogel accelerates angiogenesis by upregulating expression of hypoxia-inducible factor-1 and angiogenic growth factors, resulting in collagen deposition and rapid wound closure. This multifunctional hydrogel can not only self-adaptively change the microenvironment to a pro-healing state by decreasing oxidative stress, but also respond to matrix metalloproteinases to release DFO. The self-adaptive multifunctional hydrogel has a potential for treating diabetic wounds.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Weiguang Zhao,Shiyi Cao,Haoxin Cai,Yang Wu,Qi Pan,Heng Lin,Jun Fang,Yinyan He,Hongbing Deng,Zhihong Liu
Reconstruction of multilayered and functional smooth muscle tissues remains a challenge nowadays. Cryogels possess great advantages in three-dimensional tissue regeneration owing to the interconnected macroporous structure, but their applications have been hindered because of limited mechanical properties. Inspired by the natural extracellular matrix, cellulose acetate electrospun nanofibers (NFs) were incorporated to chitosan/silk fibroin (CS/SF) cryogel scaffolds to address this problem in this work. Compared with pure CS/SF scaffolds, CS/SF/NFs composite scaffolds showed roughened surface and enlarged pore size (216.2 ± 65.3 μm vs. 263.1 ± 75.7 μm) allowing for cell adhesion and proliferation. Incorporation of nanofibers upgraded the mechanical properties of scaffolds with a six-fold increase in compressive modulus. The proliferation and infiltration of smooth muscle cells (SMCs) were remarkably improved with the existence of nanofibers. Besides, SMCs cultured on CS/SF/NFs scaffolds displayed higher expression of contraction-related genes, verifying their potential for smooth muscle tissue engineering.
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