CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2016
·Vol. 32
·期 6
·943-946
·
IF 1.086
Li Min,Jin Yongri,Wang Xiaozhong,Wu Qian,Liu Ying,Li Peng,Li Xuwen
A new triterpenoid glycoside( 1 ) and seven known triterpenoid glycosides, pseudoginsenoside RT 2 ( 2 ), yesanchinoside R 2 ( 3 ), vinaginsenoside R13( 4 ), vinaginsenoside R8( 5 ), notoginsenoside E( 6 ), 6′′′- O -acetylginsenoside Re( 7 ), 6″- O -acetylginsenoside Rb 1 ( 8 ), were isolated from the rhizomes of Panacis majoris . The new triterpenoid glycoside was elucidated as 3- O -[ β - D -glucopyranosyl-(1→2)- β - D -(6′- O -ethyl)-glucuronopyranosyl]-oleanolic acid-28- O - β - D -glucopyranoside by extensive spectroscopic and phytochemical methods. Compounds 2 ― 8 were obtained from the plant for the first time. Compounds 3 and 4 displayed good activities against adenosine diphosphate (ADP)-induced platelet aggregation, and compounds 1, 5, 6 and 8 showed moderate activities. Compound 6 exhibited moderate antiplatelet aggregation activity induced by arachidonic acid(AA).
研究领域:
天然产物化学
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药用植物学
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心血管药理学
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药物代谢
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分子药理学
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2017
·
IF 3.255
Yang Huang,Tingting Zhang,Yumei Zhao,Haibo Zhou,Guangyun Tang,Marianne Fillet,Jacques Crommen,Zhengjin Jiang
Nucleobases, nucleosides and ginsenosides, which have a significant impact on the physiological activity of organisms, are reported to be the active components of ginseng, while they are less present in ginseng extracts. Few analytical methods have been developed so far to simultaneously analyze these three classes of compounds with different polarities present in ginseng extracts. In the present study, a simple and efficient analytical method was successfully developed for the simultaneous separation of 17 nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The effect of various experimental factors on the separation performance, such as the column type, temperature and backpressure, the type of modifier and additive, and the concentration of make-up solvent were systematically investigated. Under the selected conditions, the developed method was successfully applied to the quality evaluation of 14 batches of ginseng extracts from different origins. The results obtained for the different batches indicate that this method could be employed for the quality assessment of ginseng extracts.
研究领域:
分析化学
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植物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2019
·
IF 3.202
Nan Zhao,Mengchun Cheng,Shuai Huang,Dan Liu,Qiang Zhao,Yunpeng Bai,Xiaozhe Zhang
When characterizing components from ginseng, we found a vast number of multicharged anions presented in the liquid chromatography–mass spectrometry (LC-MS) chromatograms. The source of these anions is unclear yet, while ginsenosides, the major components of ginseng, are the main suspected type of molecules because of their sugar moiety. Our investigation using 14 pure ginsenosides affirmed that the multicharged anions were formed by ginsenosides rather than other types of ingredients in ginseng. Various anions could be observed for each ginsenoside. These anions contain ions ([M-2H]2−, [M+Adduct]2−), as well as those formed by polymerization of at least two ginsenosides, such as [nM-2H]2−, [nM-H+Adduct]2−, and [nM-3H]3−. The presence of so different types of ions from a ginsenoside explains the reason for the large number of anions in the LC-MS analysis of ginseng. We further found that formation of [nM-2H]2− ions was influenced by the number of sugar chains: ginsenosides containing two sugar chains produced all [nM-2H]2− ion types, whereas ginsenosides containing one sugar chain did not produce [2M-2H]2−. Thus, [2M-2H]2− and [3M-2H]2− can be utilized to rapidly identify monodesmosidic and/or bidesmosidic ginsenosides as joint diagnostic anions. The position of the glycosyl radical might be the key factor affecting the formation of multicharged multimer ions from monodesmosidic ginsenosides. Consequently, three groups of ginsenoside isomers were differentiated by characteristic [nM-2H]2− anions. Using concentration-dependent characteristics and collision-induced dissociation (CID), we confirmed that [nM-2H]2− ions are non-covalently bound multimers whose aggregation has marked distinction between monodesmosidic and bidesmosidic ginsenosides, accounting for the differentiated formation of [nM-2H]2− between them.
研究领域:
分析化学
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药物分析
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质谱学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 12
·2067
·
IF 2.861
Fan-Cheng Meng,Qiu-Shuang Wu,Ruibing Wang,Shao-Ping Li,Li-Gen Lin,Ping Chen,Qing-Wen Zhang
Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root ofP. japonicusC.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines.Keywords:Panax japonicusC. A. Mey.;Panacis Japonici Rhizoma;triterpenoid saponin;ginsenoside;chikusetsusaponin;pseudoginsenoside;standardized reference fraction;quantification;quality control;HPLC
研究领域:
中药学
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植物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Tao Li,Yongtao Yan,Linlin Han,Mingkun Li,Shiyu Liu,XinYang Zhou,Jungjoon Lee,Xiaomin Li,Yuqing Zhao
Rare saponins show numerous significant biological activities , however, there has been no research on using ginseng fruit rare saponins (GFRS) to minimize skin wrinkles. This paper studied the anti-wrinkle effect, related mechanism, material basis of efficacy, and structure-activity relationship of GFRS in vivo and in vitro . The findings demonstrate that GFRS has potent collagenase and elastase inhibition capabilities in addition to superior ultraviolet absorption properties. Secondly, GFRS can strengthen the endogenous antioxidant defense system, thus lessening the UVB-induced ultraviolet damage that L929 cells and zebrafish experience. Matrix metalloproteinases (MMPs) studies have shown that GFRS could significantly inhibit the UVB-induced MMP1 and MMP9 proteins production. Finally, the main ginsenoside component in GFRS was identified by LC-MS, and 16 kinds of ginsenoside were analyzed, including rare ginsenoside such as Rg 6 , F4, and R/S-Rg 3 , which may be caused by the transformation of prototype saponins Re and Rd caused by citric acid heat treatment (CAHT). After doing a quantitative HPLC analysis on twelve of them, it was discovered that CAHT could enrich the content of rare saponins in GFS. By evaluating these ginsenoside’s anti-wrinkle properties. It is found that the main material basis for GFRS’s anti-wrinkle action may be ginsenoside Rg 6 or F4. In summary, GFRS exhibits promising properties as an anti-wrinkle treatment because of its robust anti-oxidation, anti-collagenase, anti-elastase activity, favorable ultraviolet absorption effect, and capacity to regulate MMPs in vivo and in vitro models.
研究领域:
药理学
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皮肤科学
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植物化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Wenqi Xu,Cuicui Duan,Fumin Ma,Dan Li,Xiaolei Li
To convert ginsenosides Rb1, Rb2, Rb3, and Rc into Rd by a single enzyme, a putative β-glycosidase (Pxbgl) from the xylan-degrading bacterium Petroclostridium xylanilyticum was identified and used. The kcat/Km value of Pxbgl for Rb3 was 18.18 ± 0.07 mM–1/s, which was significantly higher than those of Pxbgl for other ginsenosides. Pxbgl converted almost all Rb3 to Rd with a productivity of 5884 μM/h, which was 346-fold higher than that of only β-xylosidase from Thermoascus aurantiacus. The productivity of Rd from the Panax ginseng root and Panax notoginseng leaf was 146 and 995 μM/h, respectively. Mutants N293 K and I447L from site-directed mutagenesis based on bioinformatics analysis showed an increase in specific activity of 29 and 7% toward Rb3, respectively. This is the first report of a β-glycosidase that can simultaneously remove four different glycosyls at the C–20 position of natural PPD-type ginsenosides and produce Rd as the sole product from P. notoginseng leaf extracts with the highest productivity.
研究领域:
生物化学
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生物技术
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药理学
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微生物学
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DOI
PubMed
高影响因子
PLoS One
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2022
·Vol. 17
·期 6
·e0269238
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IF 3.752
Juxin Yin,Jianjian Zhuang,Xin Zhang,Chaojian Xu,Shaowu Lv
Heavy-metal pollution has been established to affect ginseng quality. However, this effect is still unknown in ginseng of different ages, emphasizing the need to investigate the effects of heavy metals in soils on ginseng growth. Herein, we determined the content of heavy metals (Cu, Cd, Pb, Hg, and As) in ginseng of different ages (2 to 6-year-old) and the corresponding soil samples. Then, the total ginsenosides content of ginseng and rate-limiting enzyme (HMGR, SQE, CYP450) activity in the synthesis of ginsenosides were assessed. Results from 200 differently-aged Chinese ginseng showed that increased ginsenoside content in 3 to 5-year-old ginseng was paralleled by increased heavy metal element content in ginseng and its soil. The activity of rate-limiting enzymes increased in the first four years of ginseng growth and then exhibited a steady or downward trend. Further analysis suggested that heavy metal elements in soils could directly affect ginsenoside content. Moreover, we found that Cu significantly affected the rate-limiting enzyme CYP450 activity. Further principal component analysis and correlation analysis found that heavy metals could obviously inhibit ginseng growth during the 5th and 6th years. Heavy metal content in soils has huge prospects for predicting ginsenoside, Cu and As content in ginseng. This study provided support for ginseng cultivation, quality research and quality assessment.
研究领域:
植物学
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药理学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2022
·
IF 6.212
Ze-Yan Chen,Xu-Ya Wei,Ting Tan,Yu-Lin Feng,Juan Guo,Guang-Hong Cui,Chang-Jiang-Sheng Lai,Lu-Qi Huang
There is no effective method for unified quality evaluation for the same medicinal materials in similar prescriptions. In this study, we used Ginseng-Fuzi ( Aconitum carmichaelii ) proprietary Chinese medicines (GFPCMs), with different dosage forms, as examples to develop a new unified quality control strategy. Thus, using a high performance liquid chromatography-tandem mass spectrometry method, coupled with unified quality evaluation strategies to comprehensively characterize the contents of 12 aconitines and 25 ginsenosides containing eight groups of isomers in 10 GFPCMs. Quality control was carried out for the first time for aconitines and ginsenosides in Jianbuqiangshen Pill, Nanbao Capsule, Nvbao Capsule, Pingfengshengmai Capsule, Wumei Pill, and Xinbao Pill. Furthermore, 4 aconitines and 16 ginsenosides were firstly quantified in all 10 GFPCMs. Subsequently, there was a comprehensive comparison of the universality and accuracy of the quality control strategies based on absolute, normalized, and unit content. It was indicated the strategy-based unit content was more practical and effective. As for Fuzi, the total unit content of diester alkaloids should be less than 0.020%, while monoester alkaloids should be more than 0.010%. As for ginseng, the total unit content of Rb1, Rb2, Rc, Rg5, and Re should be higher than 2.0%, and the unit content of Rg5 was a good indicator for the classification of the dosage forms and processing methods. In summary, this strategy showed great potential for supervising the quality of Chinese medicine.
研究领域:
药理学
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分析化学
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中药学
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产品关联
DOI
高影响因子
Food Frontiers
·
2025
·
IF 6.9
Shu-zhen Ran,Rui Peng,Qin-wan Guo,Li-juan Ma,Jian-bo Wan,Jun-qing Huang,Zi-ying Wang,Gang Chen
Panax notoginseng Leaf (PNL), an edible-medicinal botanical resource, shows potential as functional ingredients for alleviating depression, owing to their unique saponin composition. This study investigates the regulatory effects of PNL-derived saponins on pituitary adenylate cyclase-activating polypeptide (PACAP) and their related antidepressant mechanisms. Our findings demonstrate that PNL supplementation significantly alleviates depressive-like behaviors. Mechanistically, PNL upregulates PACAP expression in the hippocampus and activates the downstream CREB and mTOR signaling pathways, leading to increased expression of synaptic plasticity markers, including BDNF and PSD95, while concurrently inhibiting microglial activation and the production of pro-inflammatory cytokines. Among the major saponins in PNL, ginsenosides Rb3 and Rc were found to synergistically enhance PACAP expression in the cultured PC12 cells. These results underscore the potential of PNL for novel functional food ingredients, highlighting the antidepressant synergism of its naturally occurring ginsenosides (Rb3/Rc), and provide a natural antidepressant strategy.
研究领域:
药理学
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神经科学
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植物学
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生物化学
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精神病学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Shuyun Zhu,Zhenjia Zheng,Hongwei Peng,Jing Sun,Xian-En Zhao,Huwei Liu
This work proposed a novel strategy based on quadruplex stable isotope derivatization for sensitive, accurate, speedy and green determination of panaxadiol (PD) and panaxatriol (PT). This strategy integrated with ultrasound-assisted dispersive liquid-liquid microextraction, microwave-assisted derivatization, and magnetic graphene oxide dispersive solid phase extraction as efficient sample pretreatment techniques, coupling with ultra-high performance liquid chromatography tandem mass spectrometry detection in multiple reaction monitoring mode for the analytical purpose of high-throughput, sensitivity, selectivity, green and accuracy. Quadruplex mass spectrometry sensitizing derivatization reagents, 3- N -( D 0 -/ D 3 -methyl-, and D 0 -/ D 5 -ethyl-)-2′-carboxyl chloride rhodamine 6 G, were designed, synthesized and applied for the high-throughput derivatization of hydroxyl‑containing PD and PT for the first time. The analogue derivatives of PD and PT standards using 3- N -propyl-2′-carboxyl chloride rhodamine 6 G as derivatization reagent were employed as the internal standards for the mass spectrometry quantitation to minimize matrix effect. All rhodamine 6 G derivatives of PD and PT were easily and selectively adsorbed by magnetic graphene oxide for efficient magnetic dispersive solid phase extraction before injection. Parameters of (micro-) extractions, derivatization and liquid chromatography-mass spectrometry conditions were all optimized in details. Method validation results consisting of linearity, accuracy, precision, repeatability, matrix effect, stability and limits of detection (LODs, 0.02 ng/mL for PD and 0.03 ng/mL for PT) were determined. The analytical performance of this method benefited from efficient integration of suitable sample pretreatment techniques and characteristic structure of rhodamine 6 G with four mass tags in derivatization reagents. The developed and validated method has been applied to the quantitation of PD and PT for the quality assessment of related foodstuffs and traditional Chinese medicinal materials.
研究领域:
分析化学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2018
·
IF 0.827
Yang-Fang He,Han-Qing Cai,Wen-Ying Li,Yang Xiu,Wen-Long Liu,Hong-Yue Chi,Hong Shen,Mao-Guang Yang,Jin Pei,Shu-Ying Liu
A quantitative analysis of ginseng chemical ingredients was established using UPLC-MS, in which the concentrations of ginsenosides, oligosaccharides, and amino acids in ginseng were determined. Unlike the C18 column, which showed good performance in the determination of ginsenosides, the NH2 column was used in this method, and it exhibited good separation abilities for three types of compounds, including 6 saponins, 4 oligosaccharides, and 15 amino acids. For each type of compound, a recovery of 88%–111%, with relative standard deviations (RSD) better than 6% (n＝6) were obtained; the limits of quantification (LOQ) for three types of substances were less than 1μg/mL. Furthermore, the method showed strong ability on the discrimination Asia Ginseng and America Ginseng, and was confirmed to be robust, selective, sensitive, rapid and inexpensive.GRAPHICAL
研究领域:
药用植物学
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分析化学
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中药学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 18
·9668
·
IF 5.924
Xiaoxuan Yu,Hui Li,Dongfa Lin,Weizhuo Guo,Zhihao Xu,Liping Wang,Shuwen Guan
Panax ginsengis a valuable traditional Chinese medicine in Northeast China. Ginsenoside, the active component of ginseng, has not been investigated much for its effects on aging and its underlying mechanism(s) of action. Here, we investigated the effects of total ginsenoside (TG), a mixture of the primary active ginsenosides fromPanax ginseng, on the lifespan ofCaenorhabditis elegans(C. elegans). We found that TG extended the lifespan ofC. elegansand reduced lipofuscin accumulation. Moreover, TG increased the survival ofC. elegansin response to heat and oxidative stress via the reduction of ROS. Next, we used RNA-seq to fully define the antiaging mechanism(s) of TG. The KEGG pathway analysis showed that TG can prolong the lifespan and is involved in the longevity regulating pathway. qPCR showed that TG upregulated the expression ofnrh-80,daf-12,daf-16,hsf-1and their downstream genes. TG also reduced the fat accumulation and promoted lipid metabolism. Moreover, TG failed to extend the lifespan ofdaf-16andhsf-1mutants, highlighting their role in the antiaging effects of TG inC. elegans. The four main constitution of TG were then confirmed by HPLC and included ginsenoside Re, Rg1, Rg2and Rd. Of the ginsenosides, only ginsenoside Rd prolonged the lifespan ofC. elegansto levels comparable to TG. These findings provided mechanistic insight into the antiaging effects of ginsenoside inC. elegans.Keywords:Panax ginseng;lifespan;lipid metabolism;stress-resistant;Caenorhabditis elegans
研究领域:
药理学
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衰老研究
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）