CARBON
·
2014
·
IF 6.16
Deli Xiao,Danhua Yuan,Hua He,Chuong Pham-Huy,Hao Dai,Cuixia Wang,Chan Zhang
A novel mixed hemimicelles solid phase extraction (SPE) based on magnetic carbon nanotubes (MCNTs) and ionic liquid (IL) is developed for the simultaneous extraction and determination of trace flavonoids in spiked human urine. In this novel SPE, the formation of C 16 mimBr with mixed hemimicelles on the surface of MCNTs@SiO 2 nanoparticles (NPs) causes retention of analytes by strong hydrophobic, π–π and electrostatic interactions . This SPE technique combines the advantages of mixed hemimicelles and MCNTs. In order to establish the guidelines for the preparation of mixed hemimicelles on composite materials, different main factors affecting the preconcentration of analytes, such as material type , amount of surfactant , pH of solution, ionic strength , extraction time, desorption condition and regeneration property, were investigated and optimized. Under the selected conditions, the accuracy of the method was evaluated by recovery measurements on spiked samples, and good recoveries with low relative standard deviations from 3.5% to 4.9% were achieved. No interferences were caused by endogenous compounds in human urine. For our knowledge, this is the first development of a mixed hemimicelles SPE based on MCNTs and IL for the extraction of trace analytes in complex biological samples.
研究领域:
分析化学
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药物分析
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 14
·2400003
·
IF 2.8
Kunlin He,Qian Chen,Xueling Chen,Chungu Zhang,Shun Feng,Lianhai Shan
Furosemide (FUR), banned in sports events by the World Anti-Doping Agency, is a key target in drug tests, necessitating a pretreatment material capable of selectively, rapidly, and sufficiently separating/enriching analytes from complex matrices. Herein, a metal-mediated magnetic molecularly imprinted polymer (mMIP) was rationally designed and synthesized for the specific capture of FUR. The preparations involved the utilization of chromium (III) as the binding pivot, (3-aminopropyl)triethoxysilane as functional monomer, and Fe 3 O 4 as core, all assembled via free radical polymerization. Both the morphologies and adsorptive properties of the mMIP were characterized using multiple methods. The resulting Cr(III)-mediated mMIP (ChM-mMIP) presented excellent selectivity and specificity toward FUR. Under optimized conditions, the adsorption capacity reached 128.50 mg/g within 10 min, and the imprinting factor was 10.41. Moreover, it was also successfully applied as a dispersive solid-phase extraction material, enabling the detection of FUR concentration as low as 20 ng/mL in human urine samples when coupled with a high-performance liquid chromatography/photodiode array. Overall, this study offers a valuable strategy for the development of novel recognition material.
研究领域:
分析化学
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材料科学
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药学
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体育科学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Quan Song,Yunsu Ma,Xu Wang,Tang Tang,Yueyue Song,Yujie Ma,Guanhong Xu,Fangdi Wei,Yao Cen,Qin Hu
In this work, a novel strategy for Zn 2+ detection was established by using an “on-off-on” ratiometric fluorescent nanosensor with the help of ethylene diaminetetraacetic acid (EDTA), a common metal chelating agent. The simple and practical ratiometric fluorescent nanosensor was synthesized by covalently binding CdTe quantum dots (QDs) and carbon dots (CDs), possessing two emission peaks at 525 nm and 450 nm under a single wavelength excitation of 360 nm, respectively. The CdTe QDs served as the response signal label, and the CDs, having no response to the analytes, acted as the reference signal. The fluorescence emission of the nanosensor was turned off to create a low-level “off” state when EDTA was added, and turned on dramatically with the addition of the target Zn 2+ . Under the optimal conditions, the change of the fluorescence intensity ratio of the nanosensor at 525 nm and 450 nm (ΔF 525 /F 450 ) had good linearity against the concentrations of Zn 2+ within a dynamic linear range of 0.50–40 μM. The limit of detection was as low as 0.33 μM (3σ/K), which was low enough for the detection of Zn 2+ in human body. The proposed method was successfully applied to the detection of Zn 2+ in human urine and plasma with RSDs less than 10%. The results show that the as-prepared QDs-CDs ratiometric nanosensor has potential application of clinical detection of Zn 2+ in the human body.
研究领域:
分析化学
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
·
2022
·
IF 6.157
Shuai Quan,Kaixiang Ji,Fangshuo Liu,Tesfaye Hailemariam Barkae,Mohamed Ibrahim Halawa,Saima Hanif,Baohua Lou,Jianping Li,Guobao Xu
Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10–1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.
研究领域:
分析化学
·
药物分析
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产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Xialin Luo,Gongke Li,Yufei Hu
In this work, a novel NH 2 -MIL-53(Al) incorporated poly(styrene-divinylbenzene-methacrylic acid) (poly(St-DVB-MAA)) monolith was prepared via chemical fabrication. Moreover, it has been efficiently applied to the in-tube solid-phase microextraction (SPME) for online coupling with high-performance liquid chromatography (HPLC) to the direct determination of five estrogens in human urine samples. The NH 2 -MIL-53(Al)-polymer monolith was suitable for in-tube SPME owing to its good permeability, high extraction efficiency, chemical stability, good reproducibility and long lifetime. The extraction conditions including extraction solvent, pH of sample solution, flow rate of extraction and desorption, and desorption volume were investigated. Under the optimum conditions, the enrichment factors were 180–304 and saturated amounts of extraction were 2326–21393 pmol for estriol , 17β-estradiol, estrone , ethinyl estradiol and progesterone , respectively. The adsorption mechanism was also explored which contributed to its strong extraction to target compounds. The proposed method had low limit of detection (2.0–40 ng/L) and good linearity (with R 2 between 0.9908 and 0.9978). Four endogenous estrogens were detected in urine samples and the recoveries of all five analytes were ranged from 75.1–120% with relative standard deviations (RSDs) less than 8.7%. The results showed that the proposed online SPME-HPLC method based on NH 2 -MIL-53(Al)-polymer monolithic column was highly sensitive for directly monitoring trace amount of estrogens in human urine sample.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Luxiu Li,Ya Cui,Lingling Ren,Chunyang Li,Yehao Liu,Zhongxiu Jin
In this work, a simple and efficient dispersive solid-phase microextraction (DSPME) method based on magnetic dummy molecularly imprinted polymer nanoparticles (MMIPs) was developed for the extraction of five phthalate monoesters (mPAEs) from human urine samples. The MMIPs synthesized by sol–gel reaction and surface imprinting were analyzed by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometry, and thermogravimetric analysis. The obtained results demonstrate that the MMIPs prepared herein are characterized by high selectivity, good adsorption capacity (124.5 mg/g), excellent reusability (over five sorption–desorption cycles), and fast adsorption rates (about 30 min to equilibrium) with respect to five mPAEs. In addition, the MMIPs can be easily separated from the sample solutions due to their good magnetic susceptibility (34.0 emu/g). Using MMIPs as sorbent material in DSPME, five mPAEs in three human urine samples were extracted, and then analyzed by high-performance liquid chromatography (HPLC). Based on the obtained results, this method shows good linearity in the range of 1–100 mg/L for monomethyl phthalate, monoethyl phthalate, and monobutyl phthalate, in the range of 1–80 mg/L for monobenzyl phthalate, and in the range of 1–50 mg/L for monoethylhexyl phthalate. Moreover, good recovery (80.0%–101.0%) of all five mPAEs was observed at three spiked level with relative standard deviations of 0.6–6.6%. Overall, the results reported herein indicate that the prepared MMIPs can be used as sorbent material for the dispersive solid-phase microextraction of phthalate monoesters in human urine samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Lei Chen,Mingyue Zhang,Fengfu Fu,Jingguang Li,Zian Lin
A rapid and facile approach was developed for the synthesis of core-shell structured magnetic covalent organic framework nanobeads by using the monodisperse Fe 3 O 4 nanoparticles (NPs) as magnetic core, and 1,3,5-triformylbenzene (Tb) and benzidine (Bd) as two building blocks (denoted as Fe 3 O 4 @TbBd), which were explored as an adsorbent for magnetic solid-phase extraction (MSPE) of estrogens from human urine sample. The core-shell structured Fe 3 O 4 @TbBd nanobeads showed some attractive features involving high specific surface area (202.18 m 2 /g), uniform pore size distribution (2.8 nm), high magnetic responsivity (41.4 emu/g), as well as excellent thermal and chemical stability, which made it an ideal adsorbent for selective isolation and enrichment of estrogens. Some parameters influencing extraction efficiency, including adsorbent dosage, extraction time, pH, ion strength, desorption solvent and desorption time were investigated systematically. Combined with HPLC–MS, a simple, fast, and sensitive method was developed for the enrichment and sensitive determination of seven estrogens, which showed good linearity (r > 0.9978) in the range of 0.005–10 μg/L, low limits of detection (0.2–7.7 ng/L, S/N = 3), low limits of quantification (0.6–25.6 ng/L, S/N = 10), high enrichment factors (75–197 fold), and good precision with relative standard deviations (RSD) lower than 4.8% for intra-day and 6.7% for inter-day. The proposed method was successfully applied to the analysis of trace estrogens in urine sample of pregnant woman with good recoveries (80.6–111.6%), demonstrating the promising potential of the Fe 3 O 4 @TbBd nanobeads as adsorbent in sample pretreatment.
研究领域:
分析化学
·
材料科学
·
环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2017
·
IF 0
Jiqing Yang,Xiaoxia Liu,Shucheng Sun,Xin Liu,Li Yang
A facile, cost-effective, and high-throughput screening method was developed for enzyme-based assays based on Robolid/Microplate (RLMP) platform. The RLMP platform is constructed by immobilizing enzyme on commercial robolids and combining it with a standard 96-well microplate to achieve high-throughput analysis. The initiation and quenching of enzymatic reaction can be performed by simply sandwiching or unsealing the enzyme-immobilized robolids and the sample-containing microplate. This platform enables measurements of multiple target analytes simultaneously based on immobilized enzymatic reactions, with analysis time independent of the number of wells in the microplate. Using urea as the model analyte, we have shown that the RLMP platform exhibits large linear detection range of up to 10 mM, fast analysis time of 30 min/96 samples, as well as good reproducibility and stability. Measurements of urea in human urine and serum samples were performed using the RLMP platform and were compared with the commercial urea test kit. A good correlation was found between the two methods. This study shows that the present RLMP platform has promising prospects for detection of clinical markers and application in disease diagnosis and biochemical analysis.
研究领域:
生物技术
·
分析化学
·
临床诊断
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DOI
PubMed
ANALYST
·
2015
·Vol. 140
·期 15
·5276-5286
·
IF 4.107
Lei Yu,Ping Liu,Ya-Lan Wang,Qiong-Wei Yu,Bi-Feng Yuan,Yu-Qi Feng
We developed a strategy for non-targeted profiling of aldehyde-containing compounds by stable isotope labelling in combination with liquid chromatography–double neutral loss scan–mass spectrometry (SIL–LC–DNLS–MS) analysis. A pair of stable isotope labelling reagents (4-(2-(trimethylammonio)ethoxy)benzenaminium halide, 4-APC and d4-4-(2-(trimethylammonio)ethoxy)benzenaminium halide, 4-APC-d4) that can selectively label aldehyde-containing compounds were synthesized. The 4-APC and 4-APC-d4 labelled compounds were capable of generating two characteristic neutral fragments of 87 Da and 91 Da, respectively, under collision induced dissociation (CID). Therefore, double neutral loss scans were carried out simultaneously to record the signals of the potential aldehyde-containing compounds. In this respect, the aldehyde-containing compounds from two samples labelled with 4-APC and 4-APC-d4 were ionized at the same time but recorded separately by mass spectrometry. The peak pairs with characteristic mass differences (n × 4 Da) can be readily extracted from the DNLS spectra and assigned as potential aldehyde-containing candidates, which facilitates the identification of the target aldehydes. 4-APC and 4-APC-d4 labelling also dramatically increased detection sensitivities of the derivatives. Using the SIL–LC–DNLS–MS strategy, we successfully profiled the aldehyde-containing compounds in human urine and white wine. Our results showed that 16 and 19 potential aldehyde-containing compounds were discovered in human urine and white wine, respectively. In addition, 5 and 4 aldehyde-containing compounds in human urine and white wine were further identified by comparison with aldehyde standards. Altogether, SIL–LC–DNLS–MS demonstrated to be a promising approach in the identification and relative quantification of aldehyde-containing compounds from complex samples.
研究领域:
分析化学
·
质谱学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Zhiqiang Ma,Lijun Yang,Yutong Wang,Mengfan Wang,Wei Qi,Zhimin He
Peptide assembly provides a new approach for the construction of enzyme-like catalysts. However, low stability is still a big challenge for the peptide-based enzyme mimics because the non-covalently driven structures are often destroyed when exposed to environmental changes. Inspired by the catalytic centre of natural horseradish peroxidase (HRP) and the biological function of dityrosine in structural proteins, in this study, we presented the construction of a stable peptide-based HRP mimic (CCA-YH) through introducing covalent bonds . CCA-YH was obtained through the co-assembly of YYHYY and hemin, followed by a ruthenium-mediated photo-crosslinking reaction. Catalytic activity assay demonstrated that CCA-YH displayed enhanced catalytic ability than the non-covalent co-assemblies (CA-YH). Remarkably, CCA-YH exhibited superior long-term stability than CA-YH and natural HRP under different pH, temperature, ion strength and organic medium environments, as well as a favorable reusability. Finally, CCA-YH was applied to the colorimetric detection of uric acid and sarcosine in human urine, which displayed satisfactory sensitivity, specificity and reproducibility. This is the first report the construction of stable peptide-based enzyme mimic based on covalent co-assembly strategy.
研究领域:
生物化学
·
分析化学
·
分子生物学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2014
·
IF 2.871
Hao Li,Qingni Yu,Bin Yang,Zhongjian Li,Lecheng Lei
Galvanostatic anodic oxidation of artificial human urine (AHU) was carried out by using boron doped diamond (BDD) and IrO 2 electrodes respectively. It was found that organic compounds in urine could be almost completely mineralized in a few hours and ammonia may be oxidized into N 2 by the electro-catalytic process. However, the oxidation processes were highly dependent on the electrode materials. On the non-active BDD electrode complete oxidation occurred, and the final oxidation products of inorganic ions, including ClO 4 − and NO 3 − , were formed. Whereas on the active IrO 2 electrode, the final products generated and left in solution were highly toxic substances, including N 2 H 4 and NO 2 − , because the oxidizing activity on the IrO 2 electrode surface is relatively low. The pH values during the degradation process were monitored and the corresponding degradation mechanisms and pathways on both electrodes are proposed based upon the measured inorganic intermediates.
研究领域:
环境科学
·
电化学
·
水处理技术
·
生物医学工程
·
分析化学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Shuming Zhang,Bixia Lin,Ying Yu,Yujuan Cao,Manli Guo,Lingling Shui
Ratiometric fluorescent probes could eliminate the influence from experimental factors and improve the detection accuracy. In this article, a ratiometric nanoprobe was constructed based on silver nanoclusters (AgNCs) with nitrogen-doped carbon dots (NCDs) and used for the detection of biothiols. The fluorescence peak of AgNCs was observed at 650 nm with excitation wavelength at 370 nm. In order to construct the ratiometric fluorescent probe, NCDs with the excitation and emission wavelengths at 370 nm and 450 nm were selected. After adding AgNCs, the fluorescence of NCDs was quenched. The mechanism of the fluorescence quenching was studied by fluorescence, UV–Vis absorption and the fluorescence lifetime spectra. The results indicated that the quenching could be ascribed to the inner filter effect (IFE). With the addition of biothiols, the fluorescence of AgNCs at 650 nm decreased due to the breakdown of AgNCs, and the fluorescence of NCDs at 450 nm recovered accordingly. Thus, the relationship between the ratio of the fluorescence intensities (I 450 /I 650 ) and biothiol concentration was used to establish the determination method for biothiols. Cysteine (Cys) was taken as the model of biothiols, and the working curve for Cys was I 450 /I 650 = 0.60C Cys − 1.86 (C Cys : μmol/L) with the detection limit of 0.14 μmol/L (S/N = 3). Then, the method was used for the detection of Cys in human urine and serum samples with satisfactory accuracy and recovery ratios. Furthermore, the probe could be applied for the visual semi-quantitative determination of Cys by naked eyes.
研究领域:
分析化学
·
纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
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