OPTICS AND LASERS IN ENGINEERING
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2020
·
IF 4.273
Hang Song,Qi Wang,Wan-Ming Zhao
In this work, we proposed a novel surface plasmon resonance (SPR) sensitivity-enhancing method by adding MoS 2 nanosheets between gold film and fiber (fiber-MoS 2 -gold film). And we apply it to the detection of human IgG protein. The sensor was fabricated by attaching MoS 2 nanosheets to the U-shaped fiber surface and then covering a layer of gold film. The refractive index sensitivity of 6184.4 nm/RIU was achieved by the fiber-MoS 2 -gold film sensor which is 59 percent higher than the gold film SPR sensor and 25 percent than the fiber-gold film-MoS 2 SPR sensor.The presence of MoS 2 increases the absorption of light energy and excites a stronger SPR phenomenon. At the same time, since the monolayer MoS 2 is a direct bandgap semiconductor, the absorbed light energy promotes the transfer of electrons, generates strong coupling on the gold film surface, enhances the surface electric field, and improves the sensitivity of the sensor. Biofunctionalized sensor detect human IgG with the limit of detection as low as 19.7 ng/mL. This work proposed a novel method for enhancing the sensitivity of SPR sensor, it owns a higher sensitivity and a lower LOD which has broad application prospects in measuring low concentration biological analytes.
研究领域:
光学
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生物传感
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纳米技术
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分析化学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qiao Liu,Xing-Li Xie,Chang-Jie Mao,Jing-Shuai Chen,He-Lin Niu,Ji-Ming Song
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available.
研究领域:
生物化学
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分析化学
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生物传感器
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免疫学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 19
·4552-4558
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IF 4.23
Xing Liu,Qi Wang,Huihui Zhao,Lichun Zhang,Yingying Su,Yi Lv
Inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of pH and temperature and lower sensitivity to proteases. Although many artificial enzymes have been investigated, searching for highly-efficient and stable catalysts is still of great interest. In this paper, we first demonstrated that bovine serum albumin (BSA)-stabilized MnO2 nanoparticles (NPs) exhibited highly peroxidase-, oxidase-, and catalase-like activities. The activities of the BSA-MnO2 NPs were evaluated using the typical horseradish peroxidase (HRP) substrates o-phenylenediamine (OPD) and 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of either hydrogen peroxide or dissolved oxygen. These small-sized BSA-MnO2 NPs with good dispersion, solubility and biocompatibility exhibited typical Michaelis–Menten kinetics and high affinity for H2O2, OPD and TMB, indicating that BSA-MnO2 NPs can be used as satisfactory enzyme mimics. Based on these findings, BSA-MnO2 NPs were used as colorimetric immunoassay tags for the detection of goat anti-human IgG in place of HRP. The colorimetric immunoassay using BSA-MnO2 NPs has the advantages of being fast, robust, inexpensive, easily prepared and with no HRP and H2O2 being needed. These water-soluble BSA-MnO2 NPs may have promising potential applications in biotechnology, bioassays, and biomedicine.
研究领域:
材料科学
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生物化学
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生物技术
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纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2011
·
IF 5.361
Cuifang Li,Zhifeng Fu,Zongyun Li,Zhenxing Wang,Wei Wei
A disposable electrochemiluminescent (ECL) immunosensor array was fabricated on a screen-printed carbon electrode (SPCE) substrate to perform multiplexed immunoassay (MIA) for the first time. The SPCE substrate was composed of an array of four carbon working electrodes, one common Ag/AgCl reference electrode, and one common carbon counter electrode. The immunosensor array was constructed by site-selectively immobilizing multiple antigens on different working electrodes of the SPCE substrate. With a competitive immunoassay format, the immobilized antigens competed with antigens in the sample to capture their corresponding tri(2,2′-bipyridyl)ruthenium(II)-labeled antibodies. The ECL signals from the immunosensors in this array were sequentially detected by a photomultiplier with the aid of a homemade single-pore-four-throw switch. Due to the ECL readout mechanism and the sequential detection mode, it could avoid the cross-talk between the adjacent immunosensors, which was common in other reported immunosensor array. Human, rabbit and mouse immunoglobulin Gs were near-simultaneously assayed as the model analytes. The linear ranges for them were 10–400, 20–400, and 20–400 ng/mL, with detection limits of 2.9, 6.1 and 6.5 ng/mL (S/N = 3), respectively. This novel ECL strategy based on immunosensor array coupled with non-array detector provided a simple, sensitive, low-cost and time-saving approach for MIA. It showed great application potential in point-of-care test and field analysis of bio-agents, with mass production potential and high throughput.
研究领域:
分析化学
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生物化学
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生物医学工程
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即时检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 7
·2355-2362
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IF 4.478
Zhao Xueqi,Zhang Yue,Wang Xinghua,Ma Pinyi,Song Daqian,Sun Ying
Long-range surface plasmon resonance (LRSPR) has demonstrated excellent performance in sensing and detection, due to its higher accuracy and sensitivity compared with conventional surface plasmon resonance (cSPR). In this work, we establish an LRSPR biosensor which employs PDA/Ti 3 C 2 -MXene/PDA-gold film as a sensing substrate and gold nanoparticles (AuNPs) as enhancers. Ti 3 C 2 -MXene is an emerging two-dimensional (2D) layered material which is used extensively in immunoassay and biosensing. The sensing substrate comprises two polydopamine (PDA) films between which is sandwiched a Ti 3 C 2 -MXene film based on a gold film, which provides a large surface area and abundant binding sites to rabbit anti-human IgG (Ab 1 ). Sandwich amplification is adopted to enhance the sensitivity of the LRSPR biosensor, and AuNPs/staphylococcal protein A (SPA)/mouse anti-human IgG (Ab 2 ) composites are introduced into the flow cell as enhancers after the immune binding of human IgG to Ab 1 . The antigen (human IgG) detection range is 0.075 μg mL −1 to 40 μg mL −1 , and the limit of detection is almost 20 times lower than that for cSPR biosensors. This novel LRSPR biosensor demonstrates excellent performance in immune sensing over a broad detection range and a low limit of detection. Subsequent modification of the LRSPR sensing platform could be made for extensive application in various biological detection fields. Graphical abstract
研究领域:
生物传感
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分析化学
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纳米技术
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免疫学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Cuixia Guo,Ziling Ye,Yonghong He,Zeyu Huang,Hongjian Zhang,Feng Huang
In response to the urgent requirement for high-throughput multiplexed detection, a novel dual-encoded suspension array (SA) based on thickness and digital fluorescent peak is proposed, along with the corresponding optical system. The SAs are prepared by thickness encoded micro-glass chips (MGCs) with electrostatically adsorbed quantum dots (QDs). Using the thickness characteristics of microcarrier itself and digital fluorescence peak (DFP) as the encoded signals ensure the high encoding stability with simple fabrication process at a low cost. A spectral-domain phase and fluorescence spectroscopy microscopy (SDPFSM) system is designed for the first time to decode the thickness information and DFP with ultrahigh accuracy. The high selectivity and sensitivity of the dual-encoded SAs are verified by the multiplexed immunoassays . The quantitative detection of anti-human IgG, anti-mouse IgG, and anti-rabbit IgG was realized with the limits of detection (LOD) of 0.19, 0.35 and 0.31 pM/mL and the sensitivity of 713, 470 and 456 a.u./(pM/mL), respectively. The coding number increases by a factor of 458 compared with the traditional fluorescence coding method due to the additional use of the thickness information of the MGCs itself as a coding channel. The aforementioned properties demonstrate that the dual-encoded SA and the corresponding decoding platform are promising and robust in multiplexed detection.
研究领域:
生物传感器
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分析化学
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生物医学工程
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光学工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Zhou Sha,Xiaochun Gao,Yijie Wang,Xiaotian Guan,Sihao Zhang,Jingru Zhao,Chunnan Wang,Shuqing Sun
Magnetic-fluorescent microbeads had been extensively used in various applications, particularly in the multiple detection of biological molecules. Currently, most commercially available magnetic-fluorescent microbeads had simple structures and the fluorescence intensity was inversely proportional to the concentration of magnetic particles, which affected performances of microbeads. In this work, a three-dimensional capillary microfluidic system was constructed to produce magnetic-fluorescent core-shell microbeads. Magnetic nanoparticles were dispersed in the core of the microbeads, while the shell consisted of quantum dots (QDs). Core-shell structures restricted fluorescence quenching caused by magnetic nanoparticles in the core area. Moreover, the number of cores could be adjusted precisely, which provided an additional encoding method besides the traditional fluorescence encoding. Three types of dual-encoded core-shell microbeads which exhibited excellent uniformity and fluorescence characteristics with additional magnetic response properties were produced. The self-assembly of silica nanoparticles on the surface of microbeads made the introduction of carboxyl groups easy. Furthermore, the capability of these microbeads for multiplex immunoassays was demonstrated using three types of IgG, and a calibration curve for the detection of human IgG was established using a sandwich immunoassay. These results indicated the potential application of this scheme in the field of biomedical detection.
研究领域:
生物技术
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纳米技术
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分析化学
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生物材料科学
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免疫测定技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
Wang Kexin,Ding Yadan,Hong Xia,Liu Yichun
A reliable, rapid and ultrasensitive immunoassay is described for determination of immunoglobulin G (IgG). It is making use of biofunctional magnetite (Fe 3 O 4 ) superparticles coated with SiO 2 and serving as an infrared (IR) probe. The unique IR fingerprint signals originating from the transverse and longitudinal phonon modes, respectively, of the asymmetric stretching of the Si–O–Si bridges display a satisfactory resistance to optical interference from the environment. The adoption of Fe 3 O 4 superparticles instead of Fe 3 O 4 nanoparticles as the magnetic core warrants a controllable structure and a strong magnetic response. This facilitates the efficient purification of the probes and the alleviation of the interfacial resistance between the liquid-solid interfaces by using a magnet. The gold-coated substrate was used to immobilize goat-anti-human IgG. The analyte (human IgG) was incubated with the IR probes, and then captured by the substrate immobilized antibody with the assistance of an external magnetic field. The integral area of the IR absorption band between 1250 cm −1 – 900 cm −1 was chosen for quantitative assay. The limit of detection is 95 fM, which is two orders of magnitude better than that without the magnetic field. The assay time was shortened from 2 h to 1 min. High selectivity, specificity, and long-term stability of the immunoassay were achieved. The performance of the assay when analyzing blood samples confirmed the practicability of the method.
Schematic presentation of the infrared (IR) immunoassay based on Fe 3 O 4 superparticle@SiO 2 nanocomposites. The assistance of an external magnetic field reduces the incubation time and improves the detection sensitivity.
研究领域:
分析化学
·
纳米技术
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生物传感器
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医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Wenwei Pan,Ziyu Han,Ye Chang,Xuexin Duan
We have developed a new three-dimensional (3D) surface for use in biosensors that is based on modified novel thorns-like polyelectrolytes (3D-PETx), which comprises of poly- L -lysine (PLL) appended with multitude oligo (ethylene glycol) (OEG) and biotin moieties. It tethered to the sensor surface by PLL, while the OEG-biotin chains are forced to stretch away from the surface for target detections. Due to its 3D structure, the number of the OEG-biotin per surface unit is markedly increased compared to conventional 2D polyelectrolytes (2D-PET) coating. Their antifouling property and sensing performance for human IgG and PSA were compared with the 2D-PET by BioLayer Interferometry (Blitz), Surface Plasmon Resonance (SPR), microfluidic devices and Enzyme-Linked ImmunoSorbent Assay (ELISA). Experimental results show that 3D-PETx presents higher sensitivity for biomarker detection both in buffer and in serum and provides an almost non-fouling surface even in undiluted serum. In addition, a sensitive PSA detection was achieved in undiluted serum with a LOD down to 0.6 ng/mL. The successful immunosensing in undiluted serum demonstrate the potential of the 3D-PETx coating for real clinical applications.
研究领域:
生物传感器
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聚合物化学
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分析化学
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生物技术
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）