MOLECULAR & CELLULAR PROTEOMICS
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2022
·Vol. 21
·期 9
·
IF 7.381
Lishan Zhang,Zujie Yao,Huamei Tang,Qingli Song,Huanhuan Song,Jindong Yao,Zhen Li,Xiaofang Xie,Yuexu Lin,Xiangmin Lin
Protein lysine acetylation (Kac) modification plays important roles in diverse physiological functions. However, there is little evidence on the role of Kac modification in bacterial antibiotic resistance. Here, we compared the differential expressions of whole-cell proteins and Kac peptides in oxytetracycline sensitive and oxytetracycline resistance (OXYR) strains of Aeromonas hydrophila using quantitative proteomics technologies. We observed a porin family protein Aha1 downregulated in the OXYR strain, which may have an important role in the OXY resistance. Interestingly, seven of eight Kac peptides of Aha1 decreased abundance in OXYR as well. Microbiologic assays showed that the K57R, K187R, and K197R Aha1 mutants significantly increased antibiotic resistance to OXY and reduced the intracellular OXY accumulation in OXY stress. Moreover, these Aha1 mutants displayed multidrug resistance features to tetracyclines and β-lactam antibiotics. The 3D model prediction showed that the Kac states of K57, K187, and K197 sites located at the extracellular pore vestibule of Aha1 may be involved in the uptake of specific types of antibiotics. Overall, our results indicate a novel antibiotic resistance mechanism mediated by Kac modification, which may provide a clue for the development of antibiotic therapy strategies.
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NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 44
·19091-19102
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IF 3.288
Faguang Ma,Yan Yan,Zhixin Yu,Yilin Wu,Xinlin Liu
The development of molecularly imprinted membranes (MIMs) has promoted applications of membrane-based separation technology, which has shown considerable advantages in water treatment, chemical separation and drug purification. Herein, imitated core–shell PEI@SiO2-based enoxacin-imprinted nanocomposite membranes (SPEIMs) were developed by mixing polyethylenimene-modified silica nanoparticles with polyvinylidene fluoride powder under freezing operation. As a typical and hazardous water pollutant, enoxacin was selected as the template molecule. Morphology and chemical characterizations were explored for an insightful analysis of the obtained membranes. Based on the organic–inorganic hybrid strategy, the as-prepared SPEIMs exhibited superior selectivity towards the target (enoxacin). Satisfactory adsorption performance (55.94 mg g−1), permeability (3.22 for norfloxacin/enoxacin and 7.07 for ciprofloxacin/enoxacin) and regeneration performance (90.31%) were achieved onto SPEIMs. The products and methods obtained in this work would potentially enrich the applications of MIMs in pollution control and biochemical separation.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Weie Wang,Qinqin Sang,Ming Yang,Juan Du,Libin Yang,Xin Jiang,Xiaoxia Han,Bing Zhao
Herein, a surface-enhanced Raman scattering (SERS) strategy based on semiconducting substrate was exploited for detection of several antibiotic residues both in ultrapure water system and in actual water system. The as-prepared Ag-TiO 2 (Ag synchronously deposited and doped TiO 2 ) nanoparticle SERS-active substrate can achieve high sensitive SERS detection for difloxacin hydrochloride, ciprofloxacin, enrofloxacin, danofloxacin and enoxacin (five widely used quinolone antibiotics) in actual water samples, and the detection limits are as low as 4.36 × 10 −12 , 7.08 × 10 −11 , 3.94 × 10 −11 , 3.16 × 10 −11 and 3.15 × 10 −10 mol/L, respectively. These detection limits are far below the maximum of residue limit (3.01 × 10 −7 mol/L) stipulated by the European Union. And, the desirable quantitative relationships can be obtained in a wide concentration range. The recoveries of five antibiotic residues from spiked actual water samples are found to be more than 80.8% with the relative standard deviations between 2.1% and 4.7%. Even, the proposed SERS method can accurately distinguish every antibiotic species from a mixed antibiotic residue sample with multiple antibiotics. And, Ag-TiO 2 nanoparticles can also serve as an efficient photocatalyst for photocatalytic degradation of these antibiotic residues, which provides a multi-functional platform for synchronous determination and degradation of antibiotic residues in real environment.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Huizhu Zhang,Yimin Tang,Weie Wang,Dongxue Yu,Libin Yang,Xin Jiang,Wei Song,Bing Zhao
Antibiotic residues in animal-derived food (egg) are threatening human health. Semiconductor heterojunction surface-enhanced Raman scattering (SERS) substrates can be used for ultra-sensitive detection of antibiotic residues in egg. Here, a TiO 2 /ZnO heterojunction was developed as a new SERS substrate based on an interface engineering strategy. Due to strong interfacial coupling and efficient carrier separating in heterostructure, utilization rate of photo-induced electrons in substrate was improved greatly, which realized the efficient charge transfer in substrate-molecule system, resulting in a prominent SERS enhancement. Taking the detection of enrofloxacin residue in egg as an example, the limit of detection (LOD) is only 13.1 μg/kg, which is far below the European Union standard, and lower than LODs of other conventional analytical methods and existing noble metal-based SERS methods. More importantly, benefiting from high sensitivity and selectivity of heterojunction and fingerprint characteristics of SERS, multiple antibiotic residues in egg can be identified simultaneously.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Ye Jiang,Xiaochan Wang,Guo Zhao,Yinyan Shi,Yao Wu
Antibiotic residues in aquaculture environments disrupt the ecosystem balance and pose a potential hazard to human health when entering the food chain. Therefore, ultra-sensitive detection of antibiotics is necessary. In this study, a multifunctional Fe 3 O 4 @mTiO 2 @Ag core–shell nanoparticle (NP), synthesized using a layer-by-layer method, was demonstrated to be useful as an enhanced substrate for in-situ surface-enhanced Raman spectroscopy (SERS) detection of various quinolone antibiotics in aqueous environments. The results showed that the minimum detectable concentrations of the six investigated antibiotics were 1 × 10 −9 mol/L (ciprofloxacin, danofloxacin, enoxacin, enrofloxacin, and norfloxacin) and 1 × 10 −8 mol/L (difloxacin hydrochloride) under the enrichment and enhancement of Fe 3 O 4 @mTiO 2 @Ag NPs. Additionally, there was a good quantitative relationship between the antibiotics concentrations and SERS peak intensities within a certain detection range. The results of the spiked assay of actual aquaculture water samples showed that the recoveries of the six antibiotics ranged from 82.9% to 113.5%, with relative standard deviations ranging from 1.71% to 7.24%. In addition, Fe 3 O 4 @mTiO 2 @Ag NPs achieved satisfactory results in assisting the photocatalytic degradation of antibiotics in aqueous environments. This provides a multifunctional solution for low concentration detection and efficient degradation of antibiotics in aquaculture water.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Zhenping Liu,Mingliang Jin,Han Lu,Jiyuan Yao,Xin Wang,Guofu Zhou,Lingling Shui
In this work, a 3D-framework of functionalized multi-walled carbon nanotubes ( f MWCNTs) decorated with molecularly imprinted polymer (MIP), has been fabricated to achieve fast and ultrasensitive electrochemical molecular sensing. The cavity networks of MIP layer from Norfloxacin (NFX) molecules offers simultaneous identification and quantification of NFX; and thus, ensures its high selectivity. The porous structure of f MWCNT 3D-framwork effectively increases the specific surface area, and the copolymer of f MWCNTs and MIP shows synergistic effect for electrocatalytic reaction of NFX at the modified sensor. Under optimized conditions, the fabricated sensors show wide linear detection ranges of 0.003-0.391 μM and 0.391–3.125 μM, low limit of determination (LOD) of 1.58 nM, and excellent selectivity in discriminating NFX according to its structural analogues and possible interferences. Precise and fast quantification of NFX in pharmaceutical formulations (Yunnan Baiyao and Ouyi Pharmaceutical) and rat plasma samples ( N = 4) suggests its simplicity and reliability. The recoveries from pharmaceutical formulations ranged from 97.36 to 109.58% with the precisions (relative standard deviations, RSD, n = 3) of 1.74–3.41%; and the recoveries from rat plasma samples ranged from 83.00 to 115.67% with RSDs ( n = 3) of 0.45–10.30%. Considering the advantages of easy fabrication, and selective and sensitive detection, such sensors with different types of MIPs would be widely applied in the fields of pharmaceutical analysis, and environmental and clinical therapeutic drug monitoring.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2019
·
IF 5.107
Jia Gao,Shi Zhou,Zhiqiang Hou,Qi Zhang,Minjia Meng,Chunxiang Li,Yilin Wu,Yongsheng Yan
In this paper, one pot-economical approach for in-situ modification of enoxacin (EXC) imprinted nanocomposite membrane was developed by employing carbon nanosphere sol (CNS) as coagulation bath during phase inversion process for increasingly antifouling property. Using polydopamine (pDA) as hydrophilic coating layer and second-reaction platform, the CNS based biomimetic molecularly imprinted nanocomposite membranes (CBMIMs) were then obtained which used EXC as the target molecule for specific adsorption and separation EXC from the sewage. The imprinting polymer layer was highly hydrophilic, which advanced antifouling performance to avoid the adsorption of contaminant protein. Using EXC as template, the optimal fabrication conditions on performances and properties of CBMIMs were studied. Isothermal adsorption curves, adsorption kinetics and rebinding selectivity were investigated to well explain the selectivity adsorption mechanism of imprinted membranes on EXC. The CBMIMs presented highly selective specificity, short kinetic equilibrium time and preferable rebinding capacity for EXC. The rebinding ability and imprinted factor ( β ) of CBMIMs could reach 32.10 mg g −1 and 3.15, respectively, which presented various highly favorable features, including high rebinding strength and excellent selective performance. Furthermore, the adsorption of actual environmental sewage by CBMIMs was investigated, which highlighted the advantage of CBMIMs. Several favorable performances of CBMIMs also provided further application for sewage treatment in the future.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-9
·
IF 5.705
Hua Jianhao,Jiao Yang,Wang Meng,Yang Yaling
The authors describe a method for the determination of norfloxacin (NOR) or ciprofloxacin (CIP). It is making use of a combination of fluorescence enhancement and magnetic solid-phase extraction (MSPE). Sulfur-doped carbon dots (S-CDs) are used as a fluorescent probe. They were prepared by a one-pot method using poly(4-styrenesulfonic acid-co-maleic acid) (PSMA) as a source for carbon and sulfur. NOR or CIP act as sensitizers of fluorescence (with excitation/emission maxima at 324/412 nm), probably due to strong hydrogen bond interaction and charge transfer with the S-CDs. The S-CDs were characterized by using TEM, XRD, XPS, FT-IR, UV-Vis and fluorescence spectroscopies. Response is linear in the 0.02–1.25 μM NOR concentration range, and the detection limit is 4.6 nM. The respective data for CIP are 0.02–1.0 μM and 6.7 nM. The average recoveries of NOR and CIP residues from spiked bovine raw milk are 96.2%~105.2% and 92.3%~102.5%.
Sulfur doped carbon dots (S-CDs) were synthesized by a hydrothermal method using poly(4-styrenesulfonic acid-co-maleic acid) (PSMA). Norfloxacin (NOR) or ciprofloxacin (CIP) was extracted by magnetic nanoparticles (MNPs), they were detected by carbon dots fluorescence enhancement.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-11
·
IF 5.3
Li Kaiwei,Jiang Han,Wang Liying,Wang Rui,Zhang Xuewei,Yang Libin,Jiang Xin,Song Wei,Zhao Bing
Semiconductor materials have become a competitive candidate for surface-enhanced Raman scattering (SERS) substrate. However, powdered semiconductors are difficult to execute a fast in situ detection for trace analytes. Here, we developed a new flexible semiconductor SERS substrate by in situ densely growing anatase TiO 2 nanoparticles on the surface of cotton fabric through a filtration-hydrothermal method, in which TiO 2 exhibits excellent controllability in size and distribution by regulating the ratio of water to alcohol in synthesis and the number of filtration-hydrothermal repetitive cycle. Cotton fabric/TiO 2 (Cot/TiO 2 ) substrate exhibits a high SERS activity and excellent spectral repeatability. The developed substrate has an ultra-high stability that can withstand long-term preservation; it can even resist the corrosions of strong acid and alkali, as well as high temperature up to 100 °C and low temperature down to − 20 °C. The flexible substrate can be used to carry out a rapid in situ detection for quinolone antibiotic (enrofloxacin and enoxacin) residues on the fish body surface by using a simple swabbing method, with high quantitative detection potential (up to an order of magnitude of 10 −7 M), and even for the simultaneous detection of both drug residues. The flexible substrate also exhibits an excellent recyclability up to 6 recycles in the actual SERS detection. Graphical Abstract
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Xin Jiang,Kaiwei Li,Yimin Tang,Xiuwen Wang,Wei Kan,Libin Yang,Bing Zhao
High-performance flexible semiconductor material can be used as an excellent multifunctional matrix for in-situ ultrasensitive surface-enhanced Raman scattering (SERS) detection and synchronous photocatalytic degradation of antibiotic residues in aquatic ecosystem. Here, a calcium-doped TiO 2 flexible matrix with double defects (surface oxygen vacancy defect and Ti 3+ energy level defect) was developed by its “in-situ one-step” hydrothermal synthesis on cotton fabric for the above purposes. Due to the joint contribution of double defects, a multi-channel charge transfer mode and a high-efficiency carrier separation are achieved, which endows flexible cotton fabric/Ca-doped TiO 2 (Cot/Ca-TiO 2 ) substrate with the greatly boosted SERS effect for in-situ detection of antibiotic residues on fish body surface and in fishpond water by a simple wiping or dipping sampling method, even for simultaneous identification of multi-component residues. The detection limits of three antibiotic residues (enrofloxacin, ciprofloxacin and enoxacin) are as low as 10 −9 M, which are far lower than the EU standard. More meaningfully, the flexible Cot/Ca-TiO 2 can be used as a multifunctional filter-membrane type photocatalyst for efficient on-site degradation of antibiotic residues in flowing fishpond water by a multi-grade photocatalysis means. Moreover, the flexible matrix exhibits good recyclability in both actual detection and photocatalysis.
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LUMINESCENCE
·
2015
·Vol. 31
·期 1
·241-246
·
IF 1.518
Qiong Yang,Xuanping Tan,Jidong Yang
A sensitive and simple method for the determination of enoxacin (ENX) was developed based on the fluorescence quenching effect of ENX for glutathione (GSH)-capped CdTe quantum dots (QDs). Under optimum conditions, a good linear relationship was obtained from 4.333 × 10 −9 mol⋅L −1 to 1.4 × 10 −5 mol⋅L −1 with a correlation coefficient (R) of 0.9987, and the detection limit (3σ/K) was 1.313 × 10 −9 mol⋅L −1 . The corresponding mechanism has been proposed on the basis of electron transfer supported by ultraviolet–visible (UV) light absorption, fluorescence spectroscopy, and the measurement of fluorescence lifetime. The method has been applied to the determination of ENX in pharmaceutical formulations (enoxacin gluconate injections and commercial tablets) with satisfactory results. The proposed method manifested several advantages such as high sensitivity, short analysis time, low cost and ease of operation. Copyright © 2015 John Wiley & Sons, Ltd.
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JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 38
·期 1
·134-140
·
IF 2.594
Dan Liu,Jiutong Ma,Yan Jin,Xiqian Li,Xiao Zhou,Qiong Jia,Weihong Zhou
This study describes the enrichment ability of ZnO-modified methacrylic acid- co -ethylene dimethacrylate polymer monoliths as stationary phases for the simultaneous determination of antibiotics (ofloxacin, ciprofloxacin, enoxacin, and pefloxacin) combined with high-performance liquid chromatography. The prepared monolith was characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier-transformed infrared spectroscopy, and thermogravimetric analysis. The polymer monolith microextraction method has been applied to the enrichment of fluoroquinolone antibiotics and satisfactory results were obtained in the analysis of water samples. Compared with the conventional methacrylic acid based monolith, the developed monolith exhibited a higher enrichment capacity because of the introduction of zinc oxide into the preparation process.
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