Journal of Environmental Chemical Engineering
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2022
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IF 7.968
Mengying Yang,Yunfan Jia,Yun Chen,Pengcheng Yan,Li Xu,Junchao Qian,Feng Chen,Henan Li
Photoelectrochemical (PEC) aptasensor is a promising analysis platform with high selectivity and sensitivity. Exploration of photoelectric active material with excellent photoelectric conversion efficiency is the key condition to design a PEC aptasensor with excellent sensitivity. Herein, a PEC aptasensor was designed and constructed by g-C 3 N 4 /Bi 24 O 31 Cl 10 heterojunction as photoelectric active material for realizing sensitive detecting enrofloxacin (ENR) antibiotic. The formation of heterojunction can facilitate separation and transfer of photogenerated carriers and reduce the recombination rate, leading to the enhancement of photocurrent response compared to that of individual semiconductor. The ENR aptamer was introduced as the specific recognition element to realize excellent selectivity of the PEC aptasensor. Because ENR-aptamer complex can block the transfer of charge, the ENR-PEC aptasensor presented the decreased photocurrent response with the increasing of ENR concentration without external voltage. The developed ENR-PEC aptasensor presented excellent sensitivity and selectivity for detecting ENR with a detection range from 0.5 to 100 fM and a low detection limit of 0.167 fM (S/N = 3). The ENR-PEC aptasensor with good reproducibility may be potential for the determination of the actual samples.
研究领域:
分析化学
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环境科学
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生物医学工程
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-11
·
IF 5.3
Liu Huamin,Li Chenghong,Wang Lina,Fang Lichao,Huang Hui,Deng Jun,Hu Yue,Li Mimi,Ran Xiaoping,Li Lulu,Zheng Junsong
A “signal-off” photoelectrochemical (PEC) sensing platform has been designed for the ultrasensitive detection of DNA methylation levels and multiple methylated sites. The platform employs tungsten trioxide and TpPa-1-COF loaded by gold nanoparticle (AuNPs@WO 3 @TpPa-1-COF) composite material as the photoactive component and p-type reduced graphene (rGO) as an efficient quencher. The PEC signal of AuNPs@WO 3 @TpPa-1-COF composite is effectively quenched in the presence of p-type rGO, because p-type rGO can compete with AuNPs@WO 3 @TpPa-1-COF to deplete light energy and electron donors. In addition, a hybrid strand reaction (HCR) amplification strategy fixes more target DNA and then combines with rGO-modified anti-5-methylcytosine antibody to facilitate ultrasensitive DNA methylation detection. Under optimal conditions, DNA methylation can be measured within a linear concentration range of 10 –14 to 10 –8 M, with an exceptionally low detection limit of 0.19 fM (S/N = 3). At the same time, the platform can conduct quantitative determination of multi-site methylation, with the linear equation △I = 44.19Log A + 61.43, and the maximum number of methylation sites is 5. The sensor demonstrates high sensitivity, excellent selectivity, and satisfactory stability. Furthermore, the proposed signal-off PEC strategy was successfully employed to detect DNA methylation in spiked human serum samples, with recoveries ranging from 93.17 to 107.28% and relative standard deviation (RSD) ranging from 1.15 to 5.49%. Graphical
研究领域:
生物化学
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分析化学
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Yong Liu,Rui Wang,Yuhan Zhu,Ruizhen Li,Jingdong Zhang
A photoelectrochemical (PEC) sensing platform was designed for catechol determination using CdS quantum dots (QDs), DNA and pristine graphene (PGR) nanocomposite film-modified electrode. In such a composite film-based PEC sensor, CdS QDs served as the photoelectric conversion element which produced photocurrent signal under visible light illumination; and DNA was designed as the biological recognition element which improved the PEC response of sensor toward catechol due to the interaction between DNA and catechol. To improve the electron transfer of the composite film, PGR with high electrical conductivity was doped, which significantly amplified the photocurrent signal of sensor. Based on such a CdS-DNA-PGR composite film-modified electrode, catechol showed a linear PEC response proportional to its concentration from 1.0 × 10 −8 to 1.0 × 10 −6 mol L −1 . The detection limit (3S/N) was estimated to be 4.9 × 10 −9 mol L −1 . The developed PEC sensor was successfully applied to determine trace catechol in water samples.
研究领域:
分析化学
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环境科学
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生物传感技术
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Jing Sun,Zhichang Ma,Hemeiling Cai,Junwei Di
A photoelectrochemical dopamine sensor based on BiOI and AuAgNPs nanoparticles modified indium tin oxide (ITO) electrode was prepared successfully. The noble metal material AuAg significantly improved the shortcomings of poor surface conductivity and easy recombination of photogenerated electron holes in BiOI. The prepared electrode generated a significantly enhanced and stable photocurrent signal under Xe lamp irradiation. In addition, in weak acidic electrolyte solution , dopamine molecules were protonated and preferent existed in the form of cations, which were easily attracted by electrostatic and adsorbed on the surface of negatively charged AuAgNPs. As an electron donor , DA is vulnerable to oxidation to DA + by BiOI holes, resulting in an increased anodic photocurrent. The detection limit was 0.3 pM (S/N = 3) and the dopamine concentration exhibited a nice linear relationship between 1 pM and 0.1 μM under ideal circumstances. The dopamine photoelectrochemical sensor shown strong sensitivity, a broad linearity range, and good selectivity against potential uric acid and ascorbic acid interference. This work provides a promising PEC detection platform for the detection of small and medium-sized molecules in biological analysis.
研究领域:
生物分析
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材料科学
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医学检测
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2023
·
IF 4.5
Hemeiling Cai,Jing Sun,Zhichang Ma,Junwei Di
An enhanced charge transfer between BiVO 4 and Au nanoparticles (AuNPs) was utilized to design a “turn-on” photoelectrochemical (PEC) biosensor for sensitive detection of target DNA. The calcination of BiVO 4 on indium tin oxide (ITO) substrate was integrated with Au nanoparticles (NPs) labelled probe DNA (pDNA-AuNPs) as photoelectrode (ITO/BiVO 4 /pDNA-AuNPs). The cathodic photocurrent increased due to the coupling BiVO 4 with AuNPs, which acted as the blank signal of this PEC platform. The probe specifically recognized and captured the target DNA (tDNA) to form double strain DNA (dsDNA) after hybridization. The photocurrent intensity of the ITO/BiVO 4 /dsDNA-AuNPs photoelectrode further increased due to the enhancement of the charge transfer between BiVO 4 and AuNPs through dsDNA chain. This photocurrent enhancement was linear proportional to the logarithm of tDNA concentration. Based on this strategy, the PEC assay displayed a linear range from 1 aM to 10 pM and the detection limit was as low as 0.2 aM (S/N = 3). It provides a promising platform for ultrasensitive target DNA detection, and thus shows a great potential in genetic disease or cancer diagnostics and infectious pathogen detection.
研究领域:
生物传感
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分析化学
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纳米技术
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产品关联
DOI
高影响因子
Biosensors-Basel
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2024
·Vol. 14
·期 6
·285
·
IF 4.9
Jie Huang,Florian Ion Tiberiu Petrescu,Bing Li,Likui Wang,Haiyan Zhu,Ying Li
The ratiometric detection method has a strong attraction for photoelectrochemical bioanalysis due to its high reliability and real-time calibration. However, its implementation typically depends on the spatial resolution of equipment and the pairing of wavelength/potential with photoactive materials. In this paper, a novel ratiometric photoelectrochemical biosensor based on front and back illumination was prepared for the detection of glutathione (GSH). Unlike traditional ratio methods, this ratiometric biosensor does not require voltage and wavelength modulation, thereby avoiding potential crosstalk caused by voltage and wavelength modulation. Additionally, the formation of a heterojunction between mTiO2and Ag2S is conducive to enhancing light absorption and promoting charge separation, thereby boosting the photocurrent signal. Apart from forming a heterojunction with TiO2, Ag2S also shows a specific affinity towards GSH, thus enhancing the selectivity of the mTiO2/Ag2S ratiometric photoelectrochemical biosensor. The results demonstrate that the ratiometric photoelectrochemical biosensor exhibits a good detection range and a low detection limit for GSH, while also possessing significant interference elimination capability. The GSH detection range is 0.01–10 mmol L−1with a detection limit of 6.39 × 10−3mmol·L−1. The relative standard deviation of 20 repeated detections is 0.664%. Impressively, the proposed novel ratiometric PEC biosensor demonstrates enviable universality, providing new insights for the design and construction of PEC ratiometric sensing platforms.
研究领域:
生物传感技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Huiyu Zou,Wuming Wu,Jingjing Zhou,Chunyan Deng
In this study, a novel integrated photoelectrochemical (PEC) sensor platform was proposed, utilizing an optical fiber (OF) as the working electrode for guided in situ light. A CdS quantum dots (QDs)/ZnO nanosheets (NSs) n-n heterojunction was quickly and easily constructed on the OF surface by successive ionic layer adsorption and reaction (SILAR). Au nanoparticles (NPs)@dsDNA as a capturing probe were modified on the CdS QDs/ZnO NSs@OF (CZ@OF). Due to the energy transfer between Au NPs@dsDNA and CdS QDs, the resultant opto-electrode has a lower background near zero, enabling the “signal-on” detection of biomarkers (interleukin-6 (IL-6) as a model). The OF-PEC biosensor demonstrated a wide linear range from 1 to 100 pg mL–1 with a regression coefficient (R2) of 0.9958 and an impressive detection limit (LOD) of 0.19 pg mL–1. More significantly, the proposed OF-PEC can be successfully used for the detection of IL-6 in serum samples from patients with pulmonary arterial hypertension, and it showed consistency and is more sensitive to trace concentrations compared to BD FACSCanto II flow cytometry used at the hospital. This holds significance for an early disease diagnosis. Therefore, the proposed OF-PEC not only achieves integration of the light source and sensing interface but also enables sensitive and accurate “signal-on” detection of IL-6. Furthermore, due to the flexibility and remote detection capabilities of OF, the application of OF-PEC is expected to be expanded more widely. This approach opens up possibilities for advances in PEC sensing.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
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IF 5.437
Kai Yan,Rui Wang,Jingdong Zhang
A novel photoelectrochemical (PEC) biosensing platform was constructed by assembling CdSe quantum dots (QDs) and DNA on liquid phase deposited TiO 2 (DNA–CdSe/TiO 2 ) film electrode. The transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis indicated that CdSe QDs were homogeneously assembled on TiO 2 film. The UV–visible diffuse reflectance spectra (DRS) showed that CdSe and DNA could effectively enhance the absorption of TiO 2 film to visible light. The obtained electrode showed a sensitive PEC response to o -aminophenol (OAP) under visible light irradiation . Due to the interaction between DNA and OAP, the response of OAP was improved by DNA immobilized on the sensing film. Under optimized conditions, the photocurrent was linearly proportional to OAP in the concentration range from 4.0×10 −7 to 2.7×10 −5 mol L −1 , with a detection limit (3 S/N) of 8.0×10 −8 mol L −1 . The novel strategy could provide a fast and sensitive method for OAP determination.
研究领域:
生物传感技术
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材料科学
·
分析化学
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DOI
高影响因子
Materials Express
·
2019
·Vol. 9
·期 7
·741-748
·
IF 1.465
Chi Kuan-Neng,Hu Rong,Yang Yun-Hui
In this work, a neoteric photoelectrochemistry (PEC) sensor on account of perylenediimide (PDI) by coupling with ascorbic acid (AA) responsive decomposition of cobalt oxyhydroxide (CoOOH) nanosheets was developed. PDI is a kind of organic photoelectric material with strong photoelectrochemical
properties in aqueous solution. When the surface of the PDI is modified by CoOOH, the photoelectric properties of the PDI could be effectively decreased. However, when AA is present, CoOOH is reduced to Co2+ while the photoelectric properties of PDI are restored. The photoelectric
sensor constructed thereby exhibits high selectivity and transient response, and is simple in operation, low in cost and short in time, and provides a novel detection method for small molecule ascorbic acid. In the case of a signal-to-noise ratio of 3, the detection limit is 33.4 nM. This
paper opens a new PEC method to detection of AA.
研究领域:
分析化学
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生物传感
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Li Li,Mingqing Li,Huan Liu,Boya Li,Baohui Wang
BiOI film was electrodeposited deposited on fluorine doped tin oxide (FTO), then it was transformed into a nanoporous BiVO 4 film by annealing at 420 °C. The BiVO 4 /FTO electrode is used as photoelectrochemical (PEC) sensor to detect ascorbic acid (AA) for the first time. Under illumination of low power 20 W LED lamp, the current density of the sensor increases with the increase of AA concentration. The proposed PEC sensor exhibited a sensitive detection for AA in a wide range from 5 μM to 300 μM with a low detection limit of 1 μM (S/N = 3) and sensitivity of 293 μA mM −1 cm −2 . A portable AA detection device was constructed with a 20W LED lamp as the light source, BiVO 4 /FTO electrode as an anode, Pt as a cathode, and ammeter as a photoelectric signal receiver. The concentration of AA detected by this device is close to that measured by the electrochemical workstation (RSD<10%), which makes the sensor practical and portable.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Wenfeng Zhuge,Xiaokun Li,Suxiang Feng
Simple, fast, and sensitive detection methods are required for reduced glutathione (GSH), a metabolic substance that acts as an indicator for a variety of diseases and is also found in numerous supplements and drugs. Herein, a sensitive and selective photoelectrochemical (PEC) sensor was developed for GSH detection. The novel PEC sensor was fabricated using CoFe 2 O 4 nanoporous spheres and copper tetraaminophthalocyanine–graphene oxide (CuTAPc-GO) as photoelectric materials. Under illumination, CoFe 2 O 4 facilitated the spatial separation of the photogenerated charge carriers in CuTAPc-GO, thereby enhancing the photocurrent signal of the PEC sensor. The influences of pH, CoFe 2 O 4 amount, coating amount, and applied bias potential on the PEC response toward GSH were investigated. Under optimized conditions, the designed PEC sensor presented a wide linear response range for GSH of 0.25–320 μM with a detection limit of 0.016 μM at a signal-to-noise ratio of 3. Moreover, this PEC sensor exhibited excellent selectivity, reproducibility, and stability, showing satisfactory results for the detection of GSH in urine and pharmaceutical drugs.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 7
·969-978
·
IF 3.1
Zuxing Zhang,Mingqing Li,Liying Zhai,Jiahui Wu,Li Li
A ternary nanocomposite photoelectrode composed of cadmium sulfide (CdS), polyaniline (PANI), and bismuth vanadate (BiVO4) was successfully designed by combining cyclic voltammetry (CV) with electrochemical deposition and high-temperature calcination. The first synthesized CdS/PANI/BiVO4 composite was used as a photoelectrochemical (PEC) monitoring platform for glutathione (GSH). The ternary CdS/PANI/BiVO4 nanocomposites exhibited higher PEC activity, which was attributed to the accelerated electron transfer by the loading of CdS and PANI, which enables the material surface to better adsorb the electrons separated by GSH, thereby oxidizing it into GSSH. The photoanodes were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis diffuse reflectance spectroscopy, and photoelectrochemical experiments. Under the optimal experimental conditions, the BiVO4 electrode modified with CdS and PANI exhibited a linear response in the concentration range of 0.1–20 μM with a sensitivity of 0.669 μA mM−1 cm−2 and a detection limit of 40 nM. Moreover, the PEC sensor exhibits good reproducibility and long-term stability. In summary, the designed materials have excellent electrochemical properties, which make them ideal candidates for PEC detection of GSH.
研究领域:
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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