APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2022
·Vol. 128
·期 4
·1-10
·
IF 2.983
Zhang Hongding,Wu Sifei,Xing Zhenhua,Gao Man,Sun Mengwei,Wang Jiaoyu,Wang Hai-Bo
Main conclusion Carbon dots (CDs) have attracted more and more attention due to their low toxicity, good biocompatibility and easy preparation. However, synthesis of CDs with high quantum yield using green and economic method is still challenging. Herein, an effective synthesis route was reported for green synthesizing CDs using waste chicken eggshell membrane as carbon source via microwave-assisted method. The obtained CDs showed good photo-stability with a high quantum yield of 19.5%. The as-prepared CDs was designed as a fluorescent probe for facile, rapid, sensitive determination of theophylline (TP) based on the fluorescence turn on–off strategy. The designed method exhibited good performance for the determination of TP. Under the optimal conditions, the fluorescent response of CDs was proportional to the TP concentrations in the range of 0.1–1 and 1–1000 μM with limit of detection of 28 nM. Moreover, the proposed method was applied to detect the TP content in tea samples. The as-prepared CDs provide a green and economic approach for the food safety test. Graphical abstract Eggshell membrane-derived carbon nanodots for direct and rapid detection of theophylline.
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高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Hui-Wen Gu,Xiao-Li Yin,Tian-Qin Peng,Yuan Pan,Hui-Na Cui,Zhi-Quan Li,Weiqing Sun,Baomiao Ding,Xian-Chun Hu,Zi-Hong Zhang,Zhi Liu
Identifying the geographical origins of green teas produced in specific regions is of significance since the geographical origin of tea influences its quality and price greatly. In this work, a novel two-dimensional (2D) fingerprints acquired by high-performance liquid chromatography-diode array detector (HPLC-DAD) was firstly proposed to identify geographical origins of Chinese green teas. A total number of 62 chemical components were extracted from 2D HPLC-DAD fingerprints of 78 tea samples by multivariate curve resolution-alternating least squares (MCR-ALS) algorithm. Afterward, principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to classify tea samples based on the extracted components. Inspection of PCA score plots of two scaling types (UV and Par) showed that tea samples from two different geographical origins have an obvious clustering tendency. As for the OPLS-DA analysis, the Q 2 cum of two types-scaling OPLS-DA models are greater than 0.75, and the total recognition rates for test set are 92.86%. What's more, according to p value of t -test, VIP values, V-plot and S-plot, 17 characteristic components were screened and identified as chemical markers to distinguish between Zhejiang teas and Shandong teas. This work indicated that the proposed strategy is suitable for identifying geographical origins of Chinese green teas.
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Food Bioscience
·
2023
·
IF 5.2
Su Zhou,Jiaying Yang,Yani Pan,Xinyu Feng,Hao Hu,Shicheng Ma,Cansong Ou,Fangyuan Fan,Shuying Gong,Yuefei Wang,Ping Chen,Qiang Chu
Numerous investigations have uncovered the outstanding therapeutic effect of tea extract on ulcerative colitis (UC). However, the therapeutic potential on UC of Pu’ er raw tea extract (PRTE) remains debated and need to be investigated. In our study, PRTE (100 and 300 mg/kg) was applied to the DSS-induced colitis mice via intragastric administration. Results have shown that PRTE treatment significantly ameliorated DSS-induced weight loss, loose stool, hematochezia, colon atrophy, splenomegaly and colon pathological injury. It could also inhibit UC by down-regulating pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and increasing the expression of tight junction protein (zonula occludens-1, ZO-1) to reinforce intestinal barrier. Fecal microbiota transplantation has further confirmed that PRTE could maintain intestinal homeostasis and relieve UC by improve the abundance of beneficial bacteria such as Lactobacillus , Muribaculum , Lachnospiraceae and Rikenellaceae , and reduce the abundance of harmful bacteria such as Turicibacter and Romboutsia . This study suggested that PRTE exhibited a noteworthy therapeutic effect on UC via restoring intestinal barrier function and maintaining gut microbiota homeostasis, which develops a safe and efficient novel strategy for UC prophylaxis and therapy, and provides theoretical support for medical application of Pu’ er raw tea in the future.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yingshuang Meng,Zhibo Qi,Zhonghua Li,Yuchao Niu,Mengyuan Wu,Zhijie Yuan,Gaohong He,Mingyang Yu,Xiaobin Jiang
Herein, a facile bionic research platform with fabricated hydrogel composite membrane (HCM) is constructed to uncover the effects of the main components of coffee’s metabolites on MSUM crystallization. Tailored and biosafety polyethylene glycol diacrylate/ N -isopropyl acrylamide (PEGDA/NIPAM) HCM allows the proper mass transfer of coffee’s metabolites and can well simulate the process of coffee’s metabolites acting in the joint system. With the validations of this platform, it is shown that chlorogenic acid (CGA) can hinder the MSUM crystals formation from 45 h (control group) to 122 h (2 mM CGA), which is the most likely reason that reduces the risk of gout after long-term coffee consumption. Molecular dynamics simulation further indicates that the high interaction energy ( E int ) between CGA and MSUM crystal surface and the high electronegativity of CGA both contribute to the restraint of MSUM crystal formation. In conclusion, the fabricated HCM, as the core functional materials of the research platform, presents the understanding of the interaction between coffee consumption and gout control.
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PubMed
高影响因子
Plants-Basel
·
2023
·Vol. 12
·期 21
·3707
·
IF 4.5
Yating Guo,Yili Shen,Boya Hu,Huichun Ye,Haowei Guo,Qiang Chu,Ping Chen
Enshi Yulu, a renowned Chinese steamed green tea, is highly valued for its unique sensory attributes. To enhance our comprehensive understanding of the metabolic variation induced by steaming fixation, we investigated the overall chemical profiles and organoleptic quality of Enshi Yulu from different tea cultivars (Longjing 43, Xiapu Chunbolv, and Zhongcha 108). The relationships between sensory traits and non-volatiles/volatiles were evaluated. A total of 58 volatiles and 18 non-volatiles were identified as characteristic compounds for discriminating among the three tea cultivars, and the majority were correlated with sensory attributes. The “mellow” taste was associated with L-aspartic acid, L-asparagine, L-tyrosine, L-valine, EGC, EC, and ECG, while gallic acid and theobromine contributed to the “astringent” taste. “Kokumi” contributors were identified as L-methionine, L-lysine, and GCG. Enshi Yulu displayed a “pure” and “clean and refreshing” aroma associated with similar volatiles like benzyl alcohol, δ-cadinene, and muurolol. The composition of volatile compounds related to the “chestnut” flavor was complex, including aromatic heterocycles, acids, ketones, terpenes, and terpene derivatives. The key contributors to the “fresh” flavor were identified as linalool oxides. This study provides valuable insights into the sensory-related chemical profiles of Enshi Yulu, offering essential information for flavor and quality identification of Enshi Yulu.Keywords:Enshi Yulu;tea cultivars;sensory traits;chemical profiles;flavor contributors
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高影响因子
ACS Sensors
·
2021
·
IF 7.711
Yintao Li,Zisheng Luo,Gangfeng Li,Tarun Belwal,Li Li,Yanqun Xu,Bin Su,Xingyu Lin
In the present study, a novel electrochemical sensor for the direct detection of caffeine in the crude sample has been prepared by plasma-triggered polydimethylsiloxane (PDMS) deposition on the indium tin oxide electrodes supported with silica nanochannels. The deposited PDMS contains both the original hydrophobic and oxidized hydrophilic PDMS oligomers. Nanochannels modified with these two kinds of PDMS with opposite wettability only allow the passage of small amphiphilic molecules such as caffeine, while other molecules including hydrophilic, hydrophobic, and large ones were all rejected. With the excellent shielding properties, the modified nanochannel electrode exhibits excellent anti-interference and antifouling capability, which could be directly used for the detection of caffeine in real crude food such as tea, milk, coffee, and coke without sample pretreatments. Moreover, the modified electrode has good repeatability and stability. In contrast, severe interference was observed when conventional electrodes were used directly in these unprocessed samples. The linear ranges of caffeine were determined to be between 50 nmol/L and 700 μmol/L, with a limit of detection of 20 nmol/L. The developed sensor provides a very simple, rapid, and cost-effective way for the interference-free and fouling-free analysis of specific amphiphilic compounds and can be extended to a wide range of applications.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Xiaoyun Jiang,Yongling Lu,Lishuang Lv
Acrolein (ACR) is found exogenously as a widespread environmental pollutant and endogenously, where it is thought to be involved as a pathogenic factor in the progression of many pathological conditions. Eliminating ACR by dietary-active substances has been found to be one potential strategy to prevent ACR-associated chronic diseases. This study first compared the scavenging ACR efficacy of four purine alkaloids, theophylline (TP), paraxanthine (PXT), theobromine (TB), and caffeine (CAF), and then, TP, CAF, and their metabolites were investigated for their ability to trap ACR in vivo. Our results indicated that TP, which possesses an −NH moiety at the N-7 position, exhibits the best ACR-trapping capacity in vitro, while CAF has a slight ability to trap ACR due to the substitutions by −CH3 at the N-1, N-3, and N-7 positions. After oral administration of TP or CAF, the ACR adducts of TP and the metabolites of TP or CAF (e.g., mono- and di-ACR-TP, mono-ACR-1,3-DMU, and mono-ACR-1-MU) were detected in urinary samples obtained from both TP- and CAF-treated mouse groups by using ultra-performance liquid chromatography–tandem mass spectrometry. The quantification studies demonstrated that TP and its metabolites significantly trapped ACR in a dose-dependent manner in vivo. Furthermore, we also detected those ACR adducts of TP and TP/CAF’s metabolites in human urine after four cups of green tea (2 g tea leaf/cup) or two cups of coffee (4 g coffee/cup) were consumed per day. Those results indicated that dietary TP or CAF has the potential capacity to scavenge ACR in vivo.
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高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Tian-Qin Peng,Xiao-Li Yin,Hui-Wen Gu,Weiqing Sun,Baomiao Ding,Xian-Chun Hu,Li-An Ma,Shu-Dong Wei,Zhi Liu,Shi-Yi Ye
Laoshan green teas plucked in summer and autumn were measured by high performance liquid chromatography-diode array detector (HPLC-DAD). After baseline correction, the fingerprints data were resolved by multivariate curve resolution-alternating least squares (MCR-ALS) and a total of 57 components were acquired. Relative concentrations of these components were afterwards applied to distinguish plucking seasons using principal component analysis (PCA), support vector machines (SVM) and partial least squares-discriminant analysis (PLS-DA). For both SVM and PLS-DA models, the total recognition rates of training set, cross-validation and testing set were 100%, 91.3% and 100%, respectively. Besides, three variable selection methods were employed to determine characteristic components for the authentication of summer and autumn teas. Results showed that PLS-DA model based on three characteristic components selected by VIP possesses identical predictive ability as the original model. This study demonstrated that our proposed strategy is competent for the authentication of plucking seasons of Laoshan green tea.
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高影响因子
COLLOID AND POLYMER SCIENCE
·
2020
·Vol. 299
·期 1
·37-47
·
IF 1.536
Wang Xiaotao,Xu Junyang,Xie Xun,Li Xuefeng,Liu Zuifang
Poly(vinyldiaminotriazine) nanoparticles adsorbed 5-fluorouracil, thymine, theobromine, and xanthine in aqueous phase. Each drug molecule formed triple hydrogen bonds with a diaminotriazine moiety at the nanoparticle surface. Adsorption isotherm studies suggested monolayer adsorption while the adsorption capacity was strongly dependent on the drug compositions/structures (0.053 mmol/g 5-fluorouracil, 0.094 mmol/g thymine, 0.0078 mmol/g theobromine, and 0.0065 mmol/g xanthine). The drug compositional/structural effects were also reflected in the adsorption kinetics. In addition to neutral pH adsorption, studies were performed at pH below the polymer’s pKa and above the drugs’ pKa, separately. Reducing hydrophobicity of the hydrogen bonding sites by protonating the diaminotriazine moieties at pH 4.5 resulted in more than 3 times decrease in adsorption capacities whereas deprotonating the drug molecules at pH 9 increased the adsorption capacity due to electrostatic attraction between the negatively charged drugs and the positively charged nanoparticles, which was supported by strong dependency of the adsorption capacity on ionic strength. The adsorption was reversible for all the four drugs. While increasing temperature caused faster desorption, reducing hydrophobicity of the diaminotriazine moieties by protonation at pH 4.5 had a stronger effect on both cumulative desorption and burst release. The results of this study demonstrated an important role of synergistic interaction between hydrogen bonding and hydrophobic affinity in poly(vinyldiaminotriazine) nanoparticle adsorption of the drugs in the aqueous phase.
Graphical abstract
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2021
·
IF 3.196
Jian Jiao,Zhiyong Zhou,Shichao Tian,Zhongqi Ren
The selective extraction of trace amounts in wastewater is necessary for the detection of water targets. In this study, theophylline-molecularly imprinted polymers (THP-MIPs) were prepared using molecular imprinting technique (MIT) for selective extraction of theophylline (THP) from wastewater. Modified silica, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) worked as functional matrix, monomers and cross-linking agents for THP-MIPs preparation. Influences of MAA dosage, cross-linking reagent dosage and cross-linking time on the adsorption capacity of synthesized THP-MIPs were analyzed. The Fourier-transform infrared (FT-IR) characterization analysis of THP-MIPs indicated the polymer was successfully synthesized. While, the thermodynamic analysis indicated the THP-MIPs had a good thermal stability below 220 °C and the THP-MIPs with the largest negative charge and the strongest electrostatic interaction at pH of 7.0. THP-MIPs reached a maximum adsorption capacity of 14.55 mg g −1 when the pH of the solution was 7.0 and the initial concentration of THP was 200 mg L −1 . The adsorption process of THP-MIPs was described using Freundlich isotherms and quasi-second-order kinetic models. The results indicated the synthesized THP-MIPs had a good selectivity and reusability in the presence of competing molecules.
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高影响因子
PLANT DISEASE
·
2019
·Vol. 103
·期 10
·
IF 3.583
Yuchun Wang,Fei Xiong,Qinhua Lu,Xinyuan Hao,Mengxia Zheng,Lu Wang,Nana Li,Changqing Ding,Xinchao Wang,Yajun Yang
Several Pestalotiopsis -like species cause gray blight disease in tea plants, resulting in severe tea production losses. However, systematic and comprehensive research on the diversity, geographical distribution, and pathogenicity of pathogenic species associated with tea plants in China is limited. In this study, 168 Pestalotiopsis -like isolates were obtained from diseased tea plant leaves from 13 primary tea-producing provinces and cities in China. Based on a multilocus (internal transcribed spacer, translation elongation factor 1-α, and β-tubulin gene region) phylogenetic analysis coupled with an assessment of conidial characteristics, 20 Neopestalotiopsis unclassified isolates, seven Pestalotiopsis species, including two novel ( Pestalotiopsis menhaiensis and Pestalotiopsis sichuanensis ), four known ( Pestalotiopsis camelliae , Pestalotiopsis chamaeropis , Pestalotiopsis kenyana , and Pestalotiopsis rhodomyrtus ) and one indistinguishable species, and three Pseudopestalotiopsis species, including two known ( Pseudopestalotiopsis camelliae-sinensis and Pseudopestalotiopsis chinensis ) and one indistinguishable species, were identified. This study is the first to evaluate Pestalotiopsis chamaeropis on tea plants in China. The geographical distribution and pathogenicity tests showed Pseudopestalotiopsis camelliae-sinensis to be the dominant cause of gray blight of tea plants in China. In vitro antifungal assays demonstrated that theobromine not only derepressed mycelial growth of the 29 representative isolates but also increased their growth. Correlation analysis revealed a linear positive relationship between the mycelial growth rate and pathogenicity ( P = 0.0148).
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2019
·
IF 2.994
Jin Tan,Rong Li,Zi-Tao Jiang,Shu-Hua Tang,Ying Wang
To circumvent the limitation of conventional high performance liquid chromatography (HPLC) method, synchronous front-face fluorescence spectroscopy was applied for the rapid and non-destructive determination of two major methylxanthines (theobromine and caffeine) and cocoa solid contents in dark chocolate for the first time. Total synchronous fluorescence spectra at wavelength interval (Δλ) between 30–100 nm of 21 brands of commercial dark chocolate bars with labeled cocoa solids of 50–100% were directly obtained at front-face geometry. The contents of the two methylxanthines were determined by HPLC. Partial least squares regression (PLSR) models for quantification were constructed, optimized, and validated by both full ( leave-one-out ) cross-validation and external validation. The coefficients of determination ( R 2 ) values are larger than 0.8, and the relative errors of prediction were ca. 10%. The built models were applied to the detection of four real samples and compared with HPLC method. The combination of front-face fluorescence and PLSR provides a fast and non-invasive procedure for the quantification of theobromine and caffeine in dark chocolate, as an effective alternative for the traditional HPLC method. The further application of the proposed strategy is the rough estimation of cocoa solids in dark chocolate.
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高影响因子
危险品化学品经营许可证（带存储）