SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhifeng Cai,Yixuan Zhang,Manlin Zhao,Jinjia Bao,Ling Lv,Haoyang Li
Copper nanoclusters (FA@CuNCs) emitting blue fluorescence were successfully developed via a one-pot technique. In this method, the copper chloride, folic acid and hydrazine hydrate were applied as a precursor, protective agent and reducing agent, respectively. The absorption, fluorescence excitation and emission spectra of FA@CuNCs were carried out by using ultraviolet–visible and fluorescence spectrometry, respectively. The morphology, particle size, functional groups, oxidation states of elements of FA@CuNCs were discussed via using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). The stability of FA@CuNCs was studied under various conditions, such as storage time at 25 ℃, ultraviolet radiation time, sodium chloride solutione and pH. The FA@CuNCs displayed blue fluorescence under the excitation wavelength of 361 nm, and the fluorescence quantum yield was 7.45 %. As a result of the inner filter effect, the alizarin red could significantly weaken the blue fluorescence of FA@CuNCs. Thus, the as-prepared FA@CuNCs could be utilized as fluorescence nanosensors for the trace determination of alizarin red. This platform suggested an excellent linear range for alizarin red varying from 0.5 to 200 μM with a fitting coefficient of 0.9955. The detection limit was calculated to be 0.064 μM in the light of the 3b/k (b and k refer to the standard deviation and slope of fitted curve, respectively). Furthermore, the as-developed FA@CuNCs could be used to detect the alizarin red in real samples and for the sensing of temperature.
研究领域:
化学
·
材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2017
·Vol. 19
·期 12
·1-11
·
IF 2.02
Chen Daquan,Song Xiaoyan,Wang Kaili,Guo Chunjing,Yu Yueming,Fan Huaying,Zhao Feng
In this article, in order to enhance the bioavailiability and tumor targeting of curcumin (Cur), the oligosaccharides of hyaluronan conjugates, folic acid-oligosaccharides of hyaluronan-acetal-menthone 1,2-glycerol ketal (FA-oHA-Ace-MGK) carried oHA as a ligand to CD44 receptor, double-pH-sensitive Ace-MGK as hydrophobic moieties, and FA as the target of folate receptor. The structure characteristics of this smart response multifunctional dual-targeting nano-sized carrier was measured by fourier-transform infrared (FT-IR) and nuclear magnetic resonance ( 1 H–NMR). Cur, an anticancer drug, was successfully loaded in FA-oHA-Ace-MGK micelles by self-assembly. The measurement results of transmission electron microscopy (TEM) presented that the Cur-loaded micelles were spherical in shape with the average size of 166.3 ± 2.12 nm and zeta potential − 30.07 mV. Much more encapsulated Cur could be released at mildly acidic environments than at pH 7.4, from the Cur-FA-oHA-Ace-MGK micelles. Cytotoxicity assay indicated that non-Cur loaded micelles mostly had no cytotoxicity to MCF-7 cells and A549 cells, and Cur-loaded micelles had significantly lower survival rate than Cur suspension in the same concentration, which proved that the drug-loaded micelles can effectively inhibit tumor cell growth. The targeting of CD44 receptors and folate receptors was proved in vitro cellular uptake assay. These results showed the promising potential of FA-oHA-Ace-MGK as an effective nano-sized carrier for anti-tumor drug delivery.
研究领域:
药学
·
肿瘤学
·
生物材料学
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产品关联
DOI
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2018
·Vol. 107
·期 2
·319-329
·
IF 3.231
Jianhui Chen,Xiaofeng Chen,Zhengyu Yang,Xiaojun Tan,Jiachen Wang,Yao Chen
We have successfully prepared a novel targeted drug delivery system, composed of folic acid (FA) as targeting molecule, methotrexate (MTX) as anticancer drug, and bioactive glass (BG) as carrier. The BG nanoparticles were synthesized by sol-gel method with the dodecylamine as template. The surface of BG nanoparticles was grafted with amino groups by (3-aminopropyl) triethoxysilane, then, FA and MTX were covalently conjugated to the surface of modified BG through amidation reaction, so FA functionalized BG (BG-FA) and targeted drug delivery system (MTX-BG-FA) were prepared. The physicochemical properties of BG, BG-NH 2 , and BG-FA were characterized by various methods, such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet–visible, and so on. Moreover, the drug release results showed that the MTX-BG-FA had sustained release property because of the peptide bond between MTX and BG-FA. And the cytocompatibility evaluation demonstrated that the BG and BG-FA were biocompatible and BG-FA even could promote cell proliferation, while the MTX-BG-FA had high cytotoxicity owning to the sustained release of anticancer drug. From the above, it could be concluded that MTX-BG-FA could kill tumor cells targetedly and sustainedly, which made it a good cancer targeted therapy material. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 319–329, 2019.
研究领域:
生物材料学
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肿瘤学
·
药剂学
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Yahui Song,Jianping Gao,Xiaoyang Xu,Huilin Zhao,Ruinan Xue,Jingkuo Zhou,Wei Hong,Haixia Qiu
Thermal sensitive supramolecular hybrid gels for injectable drug release were prepared by adding different amounts of agar into folic acid (FA) gelator. The gelation temperature was modulated in order to form injectable gel with body temperature (37 °C). Such kind of folic acid-agar (FAG) hybrid gel makes it possible to use supramolecular gel as injectable drug loaded gels for drug release. FT-IR and UV–vis spectra indicate that agar macromolecules involve in the self-assembly process through intermolecular H-bonding and π-π stacking interactions with FA molecules. The SEM and TEM images demonstrate that the fiber diameter of FAG hybrid gel is about 20 nm, much smaller than that of FA gel (40 nm). However, FAG hybrid has a denser nano-fibrous network structure than FA gels. Moreover, FAG hybrid gel is endowed with a more ordered network structure and a little better crystallization capability by adding agar. FAG hybrid gel also shows a shear-thinning behavior but the shear viscosity is about 2 times higher than that of FA gel. Compared with FA gel, the storage (G′) and loss (G″) moduli of the FAG gel are higher, which implies an enhanced gel strength. At the same time, both FA and FAG gels are facilely affected by some external factors such as acid, base and salts. In acidic or basic conditions, the strength became weak and the gelation temperature (Tg) decreased. While, within certain concentrations, the salt (NaCl) increased the gel strength and Tg. FAG gel suffered lower mass loss and owned better stability in different pH solutions compared with pure FA gel. The release behavior of the FA and injectable FAG gels was investigated by using Rhodamine B as a mimic model drug. FAG hybrid gel shows a long release profile and the release time is 3 times longer than that of FA gel, up to 30 h, and the accumulative release amount reaches about 86%. So it is a potential injectable gel for sustained release drug delivery system.
研究领域:
材料科学
·
药学
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产品关联
DOI
PubMed
高影响因子
BRAIN RESEARCH
·
2023
·
IF 2.9
Yong-Juan Gu,He-Ya Qian,Fang Zhou,Ling Zhang,Long Chen,Yu Song,Ya-Nan Chen,Hai-Long Zhang
Background Bone cancer pain (BCP) remains a clinical challenge due to the limited and side effects of therapeutic methods. Folic acid has been known as an FDA approved dietary supplement and proved to have an analgesic effect in neuropathic pain. Here we investigate the role and mechanism of folic acid in bone cancer pain of a rat model. Methods Walker 256 tumor cells were inoculated into the left tibia of rats to induce bone cancer pain model. Pain reflex were assessed by paw withdrawal threshold (PWT) response to Von Frey filaments and paw withdrawal latency (PWL) response to thermal stimulation. Folic acid was injected intraperitoneally to evaluate its analgesic effect in rats with bone cancer pain. Western blotting and qPCR were used to determine P2X2/3 receptor protein and mRNA levels in ipsilateral L4-6 dorsal root ganglion (DRG) and spinal dorsal horn (SDH). Results The PWT and PWL of rats with bone cancer pain were obviously decreased compared to the naïve and sham rats. Interestingly, continuous folic acid treatment significantly increased the PWT and PWL of rats with bone cancer pain. P2X2 and P2X3 receptors were clearly upregulated at both mRNA and protein expression in L4-6 DRG and SDH of rats with bone cancer pain. P2X2 and P2X3 receptors were mainly localized with CGRP (calcitonin gene-related peptide) or IB4 (isolectin B4) positive neurons in L4-6 DRG of rats with bone cancer pain. Notably, continuous folic acid treatment significantly reduced the expression of P2X2 and P2X3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Finally, intrathecal injection of A317491 (a selective antagonist of P2X2/3 receptors) markedly elevated the PWT and PWL of rats with bone cancer pain. Conclusion These results suggest that folic acid has an effective antinociceptive effect on bone cancer pain, which is mediated by downregulating P2X2/3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Folic acid may be a novel therapeutic strategy in cancer patients for pain relief.
研究领域:
药理学
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肿瘤学
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神经科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 24
·19662-19668
·
IF 3.289
Jing Wang,Jun Liu
Polyethyleneimine conjugated folic acid (PEI–FA) was coated onto the surfaces of fluorescent carbon nanodots (CDots) under neutral conditions through electrostatic interactions between the partially charged amino groups of PEI–FA and the carboxyl (–COOH) groups of the CDot surfaces. Because of the high abundance of folate receptors (FR) in cancer cells, folic acid (FA) was used as the targeting ligand to enhance the CDots' binding capability and penetration into the target cancer cells. We evaluated the amount of particles internalized by cancer cell lines displaying various levels of folate receptors. Two-photon excitation microscopy images revealed that the uptake of CDot–PEI–FA by FR-positive KB cancer cells was much higher than that by FR-negative A549 cancer cells. Moreover, quantitative flow cytometry analysis confirmed the receptor-mediated targeted delivery of the nanoparticles into different FR-expressing cells. The internalization of particles was ten-fold higher in the FR-positive KB cancer cells compared to that in the FR-negative A549 cancer cells; the difference was significant enough to be of biological importance. These preliminary results demonstrate the promising potential of CDot–PEI–FA for FR-positive cancer cell line diagnosis.
研究领域:
纳米技术
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癌症研究
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生物医学工程
·
分子成像
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2013
·Vol. 11
·期 1
·1-12
·
IF 0
Zhou Zhijun,Zhang Chunlei,Qian Qirong,Ma Jiebing,Huang Peng,zhang Xi,Pan Liyuan,Gao Guo,Fu Hualin,Fu Shen,Song Hua,Zhi Xiao,Ni Jian,Cui Daxiang
Background Gastric cancer is 2th most common cancer in China, and is still the second most common cause of cancer-related death in the world. Successful development of safe and effective nanoprobes for in vivo gastric cancer targeting imaging is a big challenge. This study is aimed to develop folic acid (FA)-conjugated silica coated gold nanoclusters (AuNCs) for targeted dual-modal fluorescent and X-ray computed tomography imaging (CT) of in vivo gastric cancer cells. Method AuNCs were prepared, silica was coated on the surface of AuNCs, then folic acid was covalently anchored on the surface of AuNCs, resultant FA-conjugated AuNCs@SiO 2 nanoprobes were investigated their cytotoxicity by MTT method, and their targeted ability to FR(+) MGC803 cells and FR(−) GES-1 cells. Nude mice model loaded with MGC803 cells were prepared, prepared nanoprobes were injected into nude mice via tail vein, and then were imaged by fluorescent and X-ray computed tomography (CT) imaging. Results FA-conjugated AuNCs@SiO 2 nanoprobes exhibited good biocompatibility, and could target actively the FR(+) MGC-803 cells and in vivo gastric cancer tissues with 5 mm in diameter in nude mice models, exhibited excellent red emitting fluorescence imaging and CT imaging. Conclusion The high-performance FA-conjugated AuNCs@SiO 2 nanoprobes can target in vivo gastric cancer cells, can be used for fluorescent and CT dual-mode imaging, and may own great potential in applications such as targeted dual-mode imaging of in vivo early gastric cancer and other tumors with FR positive expression in near future.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 23
·12171-12178
·
IF 7.443
Zhenxing Wang,Yanchao Xu,Yuyan Liu,Lu Shao
The current available superhydrophobic modification techniques that utilize mussel-inspired polydopamine (pDA) to construct hierarchical structures require the addition of nanoparticles or the usage of a high concentration of dopamine. These requirements are expensive and therefore lower the application efficiency. Herein, for the first time, a superhydrophobic fabric was prepared by a novel and simple mussel-inspired strategy with a much lower concentration of dopamine without any additional nanoparticles. Folic acid (FA) was first applied to a surface to induce the formation of rough pDA coatings with hierarchical structures. These hierarchical structures can be readily controlled by adjusting FA concentration or coating duration. After octadecylamine (ODA) chemical manipulation, the obtained fabric exhibited water contact and rolling off angles of about 162° and 7°, respectively, indicating that it was endowed with superhydrophobicity. Importantly, the superhydrophobic fabric can withstand continuous and drastic 3.5 wt% NaCl solution rinses and repeated tearing with an adhesive tape more than 30 times, suggesting that it has excellent durability. This novel mussel-inspired strategy can facilely and cost-effectively realize superhydrophobic manipulation and tailoring of materials. Moreover, an energy-saving and highly-efficient mini boat fabricated from our novel superhydrophobic fabric was utilized for self-driven oil spill cleanup. The boat can automatically recycle crude oil spills while floating freely on water with a cleanup rate of crude oil spill up to 97.1%, demonstrating great potential in environmental remediation. The novel strategy designed in this study will inspire the fast development of mussel-inspired superhydrophobic materials for applications in various fields.
研究领域:
材料科学
·
环境科学
·
化学工程
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产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Aihua Zou,Xiaotong Zhao,Ulrich A. Handge,Vasil M. Garamus,Regine Willumeit-Römer,Peihao Yin
Bufalin (BF), a traditional Chinese medicine, exhibited inhibitory activities against a broad spectrum of tumor cells. The present study elaborates that bufalin was successfully encapsulated into the cavity of β-cyclodextrin (β-CD), which was determined by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy ( 1 H NMR), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The best reaction mole ratio of BF/β-CD was 1:5. The solubilities of bufalin in water and phosphate buffer solution (pH = 7.4) were increased up to 24 and 34 times after encapsulated into the cavity of β-CD respectively. The inclusion efficiency (IE) and drug loading (DL) of bufalin in the inclusion complex were (94.22 ± 0.85)% and (14.11 ± 0.20)%, respectively. Then β-CD conjugated with folic acid (FA) were further prepared and employed to improve the anti-tumor efficacy of inclusion complex. The in vitro dissolution and solubility study showed better values of inclusion complex and FA targeted inclusion complex than that of pure BF. Cytotoxicity experiments by using HCT116 cell line revealed that the antitumor efficiency of bufalin were enhanced more than two folds in the presence of β-CD and folate conjugated β-CD (FA-PEI-β-CD), which demonstrated the potential application of β-CD (FA-PEI-β-CD) as delivery vehicles of bufalin for antitumor therapy.
研究领域:
药学
·
肿瘤学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yunqi Hao,Liying Yu,Tingting Li,Lihua Chen,Xu Han,Fang Chai
In this work, the blue emission carbon dots (FA-CDs) are synthesized by one-pot solvothermal method by using folic acid as precursor. The FA-CDs emitted bright emission at 445 nm when excited at 360 nm with the QY of 31.2 %. The FA-CDs exhibit sensitive quenching response to Hg 2+ with variable concentrations systematically, which determined FA-CDs can be employed as fluorescent probe, with a reliable linear relationship between fluorescence intensity and Hg 2+ concentration, and a limit of detection (LOD) of 1.29 nM. Notably, the quenched FA-CDs can be recovered by using EDTA saturated solution with the emission comparable to initial in succession. The FA-CDs based paper sensor can be explored with similar detection performance, and it can also be restored by EDTA saturated solution. Both the restored CDs and paper sensor can be reused in the next turn for detecting Hg 2+ , which allowed the FA-CDs and their paper sensor can be serviced as sustainable probe for Hg 2+ detection. The visual LOD of paper sensor can be determined at 0.1 μM, notably, the paper sensor can be reused at least 3 times with good performance, which is beneficial to environmental protection and saving resources. Possess excellent water solubility and non-toxic properties, the cellular imaging of FA-CDs was evaluated with excellent quality fluorescent image results. The FA-CDs provide a promising convenient fluorescent probe for multi-application in detection and imaging.
研究领域:
分析化学
·
环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yi Zhang,Qingbo Deng,Chang Tang,Minglu Zhang,Zilong Huang,Zhifeng Cai
Development of excellent sensors to determine trace concentrations of rifampicin is of intense importance for medicine analysis and human health. Herein, a facile and green fluorescent probe was established for the determination of rifampicin by using folic acid protected copper nanoclusters (FA-Cu NCs). Many characterization methods were applied for the analysis of the as-prepared FA-Cu NCs including UV–visible absorption spectra, fluorescence spectra, Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), fluorescence lifetime and X-ray photoelectron spectroscopy (XPS). The TEM image suggested that the as-prepared FA-Cu NCs were highly dispersed. The as-synthesized FA-Cu NCs emerged blue fluorescence under UV light and demonstrated maximum emission wavelength at 446 nm under the maximum excitation wavelength of 358 nm. After the addition of rifampicin, the FL intensities of FA-Cu NCs were uncommonly quenched. The related experimental data intimated that the quenching mechanisms were assumed to the inner filter effect (IFE) and static quenching. The as-proposed probe platform displayed an obvious linear relationship with rifampicin concentrations varying from 0.5 to 100 µM, and the corresponding detection limit (LOD) was 0.073 µM (S/N = 3). Finally, the as-established detection platform was successfully employed to analyze trace concentrations of rifampicin in real samples.
研究领域:
分析化学
·
医学检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2017
·Vol. 219
·期 4
·1700440
·
IF 2.5
Fei Peng,Liang Qiu,Ran Chai,Fanfan Meng,Chunmei Yan,Yulan Chen,Junjie Qi,Yong Zhan,Chengfen Xing
Multifunctional materials with cell imaging and therapeutic functions are anticipated to provide promising agents in cancer treatment. Herein, folic acid modified conjugated polymer nanoparticles (FA-CPNs) are exploited to target cancer cells and destroy the neighboring cancer cells. Folic acid in FA-CPNs enables the strong binding with high affinity to folate receptor, a cancer-cell-associated protein, to target cancer cells. FA-CPNs are able to produce cytotoxic reactive oxygen species upon irradiation 600 nm light, and therefore cause the cell death of folate receptor-overexpressed cancer cells effectively. Therefore, the conjugated polymer-based nanoparticles provide a multifunctional system for targeting cancer cells and photodynamic therapy.
研究领域:
纳米技术
·
癌症研究
·
诊疗学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）