FOOD CHEMISTRY
·
2023
·
IF 8.8
Dingding Liu,Kang Wei,Chenyu Zhang,Haoran Liu,Yang Gong,Yuanyuan Ye,Jiedan Chen,Mingzhe Yao,Liang Chen,Chunlei Ma
Albino tea has been receiving growing attention on the tea market due to its attractive appearance and fresh taste, mainly caused by high amino acid contents. Here, variations in the contents of five free amino acids in relation to pigment contents and tree age in two hybrid populations‘Longjin 43′(♀) × ‘Baijiguan’(♂) and ‘Longjin 43′(♀) בHuangjinya’(♂) with 334 first filial generation individuals including chlorophyll-deficient and normal tea plants were investigated. The data showed that the contents of main amino acids in all filial generation gradually decreased as plant age increased. Principal component analysis indicated that the amino acid content of individual plant tended to be stable with the growth of plants. Correlation analysis clarified that several main amino acids were significantly negatively correlated with chlorophyll a , chlorophyll b and carotenoid contents. Our results showed that the accumulation of amino acids in tea plant was closely related to leaf color variation and the tree age during growing period.
研究领域:
植物学
·
遗传学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Huan Du,Yingyun Hu,Kaixiang Lu,Tao Li,Yang Tian,Yongjin Hu
Folate is an essential vitamin for the human body and must be obtained exogenously. It is recognized that some microorganisms are capable of producing folates during growth, revealing possibilities for innovations of natural folate biofortified foods. The aim of this study was to identify potential folate-producing strains to enhance folate content in fermented Moringa oleifera leaves powder (MOLP). Although most of the tested strains exhibited folate production, there were significant differences among them in terms of both quantity and distribution, whether intracellular or extracellular. 10 strains with potential for folate production were chosen for enhancing the folate content of MOLP. The two most promising strains, Lactiplantibacillus sp. LP1 and Saccharomyces cerevisiae Y-J-1, demonstrated high folate levels up to 1940 and 2115 μg/100 g dry matter, respectively. These levels were 1.6 and 1.8-fold the initial value in MOLP. Additionally, the fermentation of MOLP not only enhanced its nutritional quality but also preserved its significant bioactivity. This study provides the initial understanding of how selected microorganisms can naturally enrich folates in fermented MOLP, presenting an alternative solution to address the widespread issue of folate deficiency while promoting human health.
研究领域:
营养学
·
微生物学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Rumeng Li,Bo Yao,Haichun Zeng
Nerol, a linear monoterpenoid, is naturally found in essential oils of various plants and is widely used in the fragrance, food, and cosmetic industries. Nerol synthase, essential for nerol biosynthesis, has previously been identified only in plants that use NPP as the precursor. In this study, a novel fungal nerol synthase, named PgfB, was cloned and characterized from Penicillium griseofulvum. In vitro enzymatic assays showed that PgfB could directly convert the substrate GPP into nerol. Furthermore, the successful expression of PgfB and its homologous protein in Saccharomyces cerevisiae resulted in the heterologous production of nerol. Finally, crucial amino acid residues for PgfB’s catalytic activity were identified through site-directed mutagenesis. This research broadens our understanding of fungal monoterpene synthases and presents precious gene resources for the industrial production of nerol.
研究领域:
真菌学
·
生物化学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Kewei Chen,Yunchang Li,Chunjie Zhou,Yuankai Wang,Zsolt Zalán,Tian Cai
Chlorophylls and β-carotene are fat-soluble phytochemicals in daily diets, while their bioaccessibility interaction remains unknown. Eight dietary chlorophylls and their derivatives (chlorophyll a , chlorophyll b , pheophytin a , pheophytin b , chlorophyllide a , chlorophyllide b , pheophorbide a , pheophorbide b ) were combined with β-carotene in six different oil matrices (corn oil, coconut oil, medium-chain triglycerides, peanut oil, olive oil and fish oil) and were subjected to in vitro digestion. Generally, chlorophylls significantly decreased β-carotene bioaccessibility by competitive incorporation into micelles. Dephytylated chlorophylls had a greater inhibitory effect on the micellarization and bioaccessibility of β-carotene compared to phytylated chlorophylls. In their co-digestion system, olive oil group exhibited the smallest particle size and biggest zeta potential in both digesta and micelles. For chlorophylls, the phytol group and their levels are key factors, which was also buttressed by the mice model where additional supplementation of pheophorbide a significantly hindered the accumulation of β-carotene and retinoids compounds.
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2020
·Vol. 7
·期 12
·3901-3913
·
IF 7.683
Jiahui Zhu,Jinfeng Li,Yu Shen,Shiqi Liu,Nengde Zeng,Xinhua Zhan,Jason C. White,Jorge Gardea-Torresdey,Baoshan Xing
Nanoparticles (NPs) are increasingly used as agrochemical components through foliar spraying such as foliage fertilizers or pesticides. However, the understanding of the mechanisms of nanoparticle absorption and translocation from the leaf surface is limited. In this study, ZnO NPs (30 nm) labeled with fluorescein isothiocyanate (FITC) were foliar applied to wheat leaf tissues to investigate the process of attachment and absorption. Using laser confocal microscopy, we observed that FITC–ZnO NPs cross the leaf epidermis through the stomata and accumulate first in the apoplast, followed by subsequent transport to mesophyll cells. The Zn concentrations in wheat leaf apoplast and cytoplasm decreased by 33.2% and 8.3% with stomatal aperture diameter reduction, respectively; the apoplastic Zn concentration is influenced more by stomatal aperture than the cytoplasmic Zn level. Scanning electron microscopy with energy-dispersive X-ray analysis was used to map Zn in the wheat leaves and data suggest a different Zn distribution for ZnO NPs and ZnSO4. Zn ions in ZnO NP-treated samples are heterogeneously distributed in comparison with those in ZnSO4-treated samples. The results indicate that the main route to cross the wheat leaf epidermis for ZnO NPs is via the stomata; then these nanoparticles accumulate and release Zn ions in the apoplast, and the released Zn ions and ZnO NPs are absorbed by mesophyll cells. Our findings demonstrate how ZnO NPs cross the wheat leaf epidermis, distribute within mesophyll tissues, and enter into plant cells, and this information is useful for the development of sustainable nano-enabled platforms for nanoscale micronutrient delivery.
研究领域:
植物科学
·
纳米技术
·
农学
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2024
·
IF 3.1
Yu Zhang,Minghui Xiao,Ranran Feng,Hong-Yu Zhang,Yuecheng Zhang,Jiquan Zhao
5-Methyltetrahydrofolate (5-MTHF) is an active form of folic acid (FA) and an essential micronutrient preventing anemia and numerous other adverse health conditions such as cardiovascular disease and cancer. Herein, a continuous flow sequence synthesis of 5-MTHF from FA was developed, involving three elementary steps: catalytic hydrogenation of FA to tetrahydrofolic acid (THFA), methylenation of THFA with formaldehyde, and hydrogenation of the methylenation intermediates to 5-MTHF. This process features an expeditious synthesis of 5-MTHF in an integrated flow platform with water as the sole solvent and without any intermediate purification. The overall yield of this three-step flow process was up to 53.54% in a total residence time of 71.4 min. The separated yield of 5-MTHF reached 49.3%.
研究领域:
生物化学
·
制药工程
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Yuying Song,Chong Cao,Kaerdun Liu,Jianbin Huang,Li Zheng,Lidong Cao,Fengmin Li,Pengyue Zhao,Qiliang Huang
Pesticide spraying droplets are prone to break and splash when they reach the leaf surface, especially for hydrophobic leaves. The lost pesticide will not deposit on the leaf surface to exert its effect, which eventually enters the environment to cause pollution and even endanger human health. The need to increase spray droplet deposition on plant leaf surfaces has been a great challenge. Many recent studies have increased the deposition of droplets by adding surfactants to spray liquids to reduce surface tension. Here, we propose a carrier system that is a coordination-triggered hierarchical folate/zinc supramolecular hydrogel loaded with pesticide active ingredient to improve the deposition of pesticide droplets. Rather than enhancing deposition by reducing the surface tension like surfactants, the pesticide-loaded folate/zinc hydrogel restricts the movement of liquid droplets through internal cross-linking to form nanofiber networks, thereby suppressing drop broken splashing and bouncing. Morever, the folate/zinc hydrogel is a biocompatible carrier with abundant raw material sources and simple preparation. The research in this paper provides new insights and directions for the study of green pesticide formulations and the enhancement of pesticide droplet deposition.
研究领域:
植物保护
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Chaozheng Zhang,Yao Chen,Yi Zuo,Maolin Wang,Hongyu Chen,Chuanqi Wang,Yuening Cao,Yingjie Zeng,Ying Chen,Tingting Zhang,Xian Ge,Xiujun Cao,Yilan Liu,Hongbin Cheng,Yun Deng,Jun Lu
Ligand-receptor recognition-based nanodrug delivery systems incorporate stimulus-responsive functionality to proactively transport chemotherapeutics to lesional tissues and intelligently release drugs in response to stimulation, thereby augmenting drug accumulation at target sites, boosting drug bioavailability, and avoiding systemic toxicity, which reveals a promising potential in malignant tumor therapy. Herein, we designed and prepared a dual-targeted nanomedicine, FA-HA-SS-PPT, utilizing microwave-assisted quantitative conjugating of podophyllotoxin (PPT) to folic acid (FA) and hyaluronic acid (HA) carriers via short-chain PEG bearing the tumor microenvironment-sensitive disulfide bond. The FA-HA-SS-PPT nanoparticles developed by self-assembly exploited the peculiarity of specifically binding to the folate receptor (FR) and CD44 receptor, initiated targeting to translocate the drug into the tumor cells and released the PPT by self-cyclization in high intracellular GSH concentration. Furthermore, this nanomedicine demonstrated superior tumor cytotoxicity and proliferation inhibition in vitro by triggering the mitochondrial apoptotic pathway and suppressing the microtubule protein formation. Notably, quantitative disulfide bond conjugation and nanomedicine formation endowed FA-HA-SS-PPT nanoparticles with astonishing in vivo circulatory stability and tumor microenvironmental responsiveness, further reinforcing drug security and anti-tumor capability. Therefore, quantitatively conjugating drugs provide a promising safe and efficient means to treat multi-receptor overexpressing tumors with multi-targeted nanomedicines.
研究领域:
药剂学
·
肿瘤学
·
生物化学
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2025
·
IF 3.6
Liang Li,Hui Jiang,Tingting Xu,Xi Wang
Citronellal and citronellol are monoterpenoids that serve as key ingredients in the flavor and fragrance industry and hold significant potential for pharmaceutical applications. Currently, these compounds are primarily obtained through chemical synthesis or extraction from plant essential oils. However, these methods raise concerns regarding environmental sustainability, carbon emissions, and the growing attentions for natural production. A promising alternative is microbial biosynthesis, which enables the natural and carbon-neutral production of these compounds in an environmentally friendly manner. In this study, we developed bienzymatic cascades for the efficient production of citronellal and citronellol from geraniol, utilizing E. coli 's endogenous glycerol dehydrogenase (GLDA), NADPH-dependent aldehyde reductase (AHR), and Catharanthus roseus iridoid synthase (CrIS). The highest in vitro production reached 92 mg/L of citronellal and 170 mg/L of citronellol in a 5-h enzymatic reaction. We also engineered these enzymatic cascades into E. coli strains for in vivo production, achieving up to 714 mg/L of citronellol. This study establishes new bienzymatic cascades for the efficient biosynthesis of citronellal and citronellol, demonstrating a possible bio-based production for natural flavor and fragrance compounds.
研究领域:
合成生物学
·
生物催化
·
代谢工程
·
工业微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemSusChem
·
2025
·Vol. 18
·期 11
·e202402588
·
IF 6.6
Shengnan Duan,Teng Gu,Chiasa Uragami,Shin-ichi Sasaki,Yuanqi Zhou,Ruohan Tao,Xianju Zhou,Chunbao Feng,Hitoshi Tamiaki,Xiao-Feng Wang,Zeyun Xiao,Hideki Hashimoto
Graphical A layered cascade bio-solar cell inspired by artificial photosynthesis is constructed using chlorophyll (Chl) and bacteriochlorophyll derivatives (BChl) to emulate PSI and PSII, respectively. Sub-ns TAS reveals a prolonged charge transfer state from BChl to Chl (Chl – /BChl + ), compared to the reverse (Chl + /BChl – ). This confirms the biomimetic device effectively replicates the natural photosynthetic electron transfer mechanism. The natural Z-scheme of oxygenic photosynthesis efficiently drives electron transfer from photosystem II (PSII) to photosystem I (PSI) via an electron transport chain, despite the lower energy levels of PSII. Inspired by this sophisticated mechanism, we present a layered cascade bio-solar cell (CBSC) that emulates the Z-scheme. In this design, chlorophyll derivatives (Chl) act as PSI analogs, while bacteriochlorophyll derivatives (BChl) serve as PSII analogs in the active layer. The resulting photocurrent, prominently detected in the near-infrared region, is validated through external quantum efficiency measurements. Sub-nanosecond transient absorption spectroscopy reveals a prolonged charge transfer (CT) state from BChl to Chl (Chl – /BChl + species) compared to the reverse direction (Chl + /BChl – species). This asymmetry highlights a dominant electron flow from BChl (PSII analog) to Chl (PSI analog) under simultaneous excitation, effectively replicating the natural Z-scheme electron transfer. These findings represent a significant advance in the design of bio-inspired solar cells, paving the way for artificial photosynthesis systems and offering profound insights into improving photovoltaic theory and efficiency.
研究领域:
生物化学
·
光合作用
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Hong Shen,Changwei Zhang,Hao zhou,Chuan Li,Hua Yuan,Jianxin Jiang,Chengzhang Wang
Chlorophyll compounds (Chls) from Ginkgo biloba leaves (GBL) are non-toxic natural pigments characterized by highly conjugated molecules and special spectra, making them ideally potential photosensitizers (PSs) in photodynamics therapy (PDT). In this study, 4 Chls were isolated from Ginkgo biloba polyprenols (GBPPs) wastes by using silica gel column separation and high performance liquid chromatography (HPLC). In addition, two new chlorophyll derivatives were successfully synthesized with chlorin e6 as the primary raw material. To evaluate and identify superior chlorophyll PSs, Density Functional Theory (DFT) and Density Functional Theory (TD-DFT)were employed to decode the microscopic and spectral properties of Ginkgo biloba Chls (GBChls). The spectral absorption, optical stability and reactive oxygen species (ROS) production of GBChls were further analyzed through comparisons of their frontier molecular orbital layout, charge distribution and excitation energy properties. Our results show that about 63 % absorption in the Q band for 14 GBChls comes from the π⇢π* transition from the highest occupied orbital (HOMO) to the lowest unoccupied orbital (LUMO), indicating the favorable delocalization effect of these GBChls. In particular, those GBChls with the long chain of the phytyl group exhibited higher total electron energy and thus could significantly increase the light stability. However, the large spatial volume and hydrophobicity of these compounds with the phytyl group chain was unfavorable for cellular uptake, leading to low ROS in cells. The electronegativity of porphin macrocycles in GBChls positively correlates with the intracellular ROS production rate, meaning that the former likely promotes the energy transfer between PSs and molecular oxygen during type II photoreaction. Among the GBChls, chlorin e6 demonstrated the highest ROS yields in DMF, while purpurin 18 compounds displayed long-wavelength absorption (698 nm), strong Q-band absorption, good optical stability, and high intracellular ROS production. Our findings highlight that chlorin e6 and purpurin 18 are most potential PSs anticancer drugs and offer new research perspectives for the processing and utilization of GBL resources.
研究领域:
植物化学
·
药物化学
·
光动力疗法
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 7
·1295-1303
·
IF 1.474
Liu Guangheng,Yang Xiangfeng,Niu Qiming,Sun Wenkui
ABSTRACT
A new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic
acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the
help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS)
and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast
agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized.
The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in
vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane,
surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.
研究领域:
肿瘤学
·
医学影像学
·
药剂学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）