FOOD CHEMISTRY
·
2023
·
IF 8.8
Siting Lai,Li Li,Qian Li,Shijiang Zhu,Guang Wang
Internal browning (IB) is a physiological disorder without external symptoms of postharvest pineapples, but whether and how IB influences pineapple volatiles remain unknown. We examined eigenvalues of volatiles in ‘Comte de Paris’ pineapples with or without IB using electronic nose (E-nose) and gas chromatography-mass spectrometry (GC–MS). Correlation coefficients between the responses of E-nose sensors S7 and S9 and IB were 0.836 and 0.804, respectively. The multilayer perceptron neural network and radial basis function neural network models classified IB degree with accuracy of 94.77% and 91.67%. GC–MS analysis revealed 30 volatile substances upregulated in pineapple with IB compared to those without, of which 15 were esters. IB regulated volatile compound synthesis through the lipoxygenase pathway which involved lipoxygenase , pyruvate decarboxylase 1, alcohol dehydrogenases , acyl-CoA oxidase 1 , and alcohol acyltransferase genes and their related enzymes. These results suggested that volatiles regulated by IB could be used to discriminate IB severity in pineapples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Yuwei Luo,Xin Chen,Jin Xin Che,Yonghua Zhang,Qiuli Ouyang,Nengguo Tao
Blue mold caused by Penicillium italicum is one of the most devastating postharvest diseases in Citrus fruit. This work aims to determine the antifungal potential of ( E )-2-octenal, a component of plant essential oil, on the mycelial growth and disease incidence of a prochloraz-resistant strain of P. italicum isolated from an orchard in Luxi, Hunan Province, China. An in vitro assay revealed that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of ( E )-2-octenal were 0.25 and 0.50 mL L −1 , respectively. An in vivo assay showed that wax + 4 × MFC ( E )-2-octenal treatment could reduce the blue mold incidence in Ponkan fruit inoculated with P. italicum without impairing fruit quality. Scanning electron microscopy analysis illustrated that the mycelia of P. italicum were anomalously twisted and shrunken upon treatment with ( E )-2-octenal. The primary antifungal targets were attributed to the cell membrane instead of the cell wall based on propidium iodide and calcofluor white staining results. In addition, ( E )-2-octenal induced massive accumulation of reactive oxygen species in hyphae in a dose-dependent manner, leading to oxidative stress and dysfunction of energy and respiration metabolism. At the same time, the activities of phosphofructokinase (PFK) and pyruvate kinase (PK) were decreased in ( E )-2-octenal-treated samples. Taken together, our present study suggested that ( E )-2-octenal could exert its antifungal activity against P. italicum by damaging the function of mitochondria and may be a potential antifungal substance for an alternative to chemical fungicides.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Junwei Yao,Heng Zhi,Qingshan Shi,Yu Zhang,Jin Feng,Jingxia Liu,Hui Huang,Xiaobao Xie
With the poorly soluble and intrinsically unstable feature, prochloraz (Pro) was confronted with lower bioavailability in the crop defense against fungal erosion. Therefore, it was a challenging project to explore the innovative antifungal compound delivery system for improving bioavailability. The superior adhesive fungicide formulation was supposed to be an efficient pathway to enhance transmembrane permeability and biological activity. According to abundant phenolic hydroxyl groups, tannic acid (TA) was an ideal modified adhesive biomaterial to improve interfacial interactions. The fundamental purpose of this research was focused on the synergistic mechanism of TA-interfacial-modified Pro-ethyl cellulose (EC) nanoparticles for improving bioavailability and biosafety. In the stability test, TA-modified Pro-EC nanoparticles had the capacity to reduce Pro initial release burst, extending a persistent validity and improving anti-photodegradation property. The toxicity index of Pro-EC and Pro-EC-TA was approximately 2.93-fold and 4.96-fold that of Pro technical against Fusarium graminearum (F. graminearum), respectively. Compared with nonmodified EC nanoparticles, TA-modified EC nanoparticles obtained eminent transmembrane permeability and superior adherence ability to F. graminearum, for hydroxyl and carboxyl groups of TA to enhance interaction with target cell membranes. The contents of cellular reactive oxygen species induced by Pro-EC and Pro-EC-TA nanoparticles were about 2.31 times and 3.00 times that of the control check (CK), respectively. Compared to the CK group, the membrane potential and ergosterol values of F. graminearum treated with Pro-EC-TA nanoparticles were drastically reduced by 74.91 and 56.20%, respectively. In the biosafety assay, the maximum half-lethal concentration value of the TA-modified Pro-EC nanoparticles indicated that the acute toxicity of the Pro-EC-TA nanoparticles to adult zebrafish was approximately 8.34-fold reduced compared to that of the Pro technical. These findings demonstrated that the successful interfacial modification of Pro-EC nanoparticles with TA was a highly efficient, environmentally safe, and promising alternative for sustainable agricultural application, thus making the fungicide formulation process more simplified, easier fabrication, and lower cost.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·
IF 5.8
Zhang Shuwen,Luo Ting,Weng You,Wang Dou,Sun Li,Yu Zheping,Zhao Yao,Liang Senmiao,Ren Haiying,Zheng Xiliang,Jin Yuanxiang,Qi Xingjiang
Carbendazim (CBZ) and prochloraz (PCZ) are broad-spectrum fungicides used in agricultural peat control. Both fungicides leave large amounts of residues in fruits and are toxic to non-target organisms. However, the combined toxicity of the fungicides to non-target organisms is still unknown. Therefore, we characterized the toxic effects of dietary supplementation with CBZ, PCZ, and their combination for 90 days in 6-week-old male Institute of Cancer Research (ICR) mice. CBZ-H (100 mg/kg day), PCZ-H (10 mg/kg day), and their combination treatments increased the relative liver weights and caused liver injury. The serum total cholesterol (TC), triglyceride (TG), glucose (Glu), pyruvate (PYR), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels were reduced, and synergistic toxicity was observed. Hepatic transcriptome revealed that 326 differentially expressed genes (DEGs) of liver were observed in the CBZ treatment group, 149 DEGs in the PCZ treatment group, and 272 DEGs in the combination treatment group. According to KEGG enrichment analysis, the fungicides and their combination affected lipid metabolism, amino acid metabolism, and ferroptosis. In addition, the relative mRNA levels of key genes involved in lipid metabolism were also examined. Compared with individual exposure, combined exposure to CBZ and PCZ caused a more obvious decrease in the expression of some genes related to glycolipid metabolism. Furthermore, the relative mRNA levels of some key genes in the combination treatment group were lower than those in the CBZ and PCZ treated groups. In summary, CBZ, PCZ, and their combination generally caused hepatotoxicity and glycolipid metabolism disorders, which could provide new insights for investigating the combined toxicity of multiple fungicides to animals.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYTOPATHOLOGY
·
2019
·Vol. 167
·期 10
·553-557
·
IF 1.097
Xiaoyu Liang,Yun Peng,Yanchao Liu,Meng Wang,Ye Yang,Yu Zhang
The rubber tree ( Hevea brasiliensis ) is the only resource for commercial natural rubber production and thus has economic importance in Southeast Asia. A spot disease on the leaves of a rubber tree was first discovered in 2017 in Hainan, China. In this study, the fungal isolate MA1 from the infected tissues was determined to be a pathogen of the spot disease by satisfying Koch's postulates. The isolate MA1 was identified as Bipolaris bicolor based on the morphological characteristics and multigene phylogenetic analysis. Among fungicides, prochloraz, iprodione and pyraclostrobin significantly inhibited hyphal growth of B. bicolor under in vitro conditions. This study constitutes the first report on the association of B. bicolor with leaf spot disease of rubber trees worldwide.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Wen Hao,Jingwen He,Jie Wu,Lin Cai,Yifei Wang,Guozhen Fang,Shuo Wang
Thiabendazole (TBZ) residues in food pose a serious threat to public health. Herein, an ultrasensitive molecularly imprinted electrochemiluminescence sensor (MIECLS) was developed to detect TBZ, using electron autoregulation in nitrogen-doped graphdiyne‑copper nanowires (NGDY-CuNWs) composite luminophore and cyclic amplification strategy of tin disulfide nanosheets (SnS 2 NSs). NGDY-CuNWs composite luminophores were formed by spontaneous chemisorption to provide electrochemiluminescence signals, and the charge redistribution in it resulted in a built-in potential that improved the electron transfer and redox reaction rate. The cyclic transformation of electron pairs (Sn 2+ /Sn 4+ ) on SnS 2 NSs catalyzed the generation of sulfate anion radicals to amplify electrochemiluminescence signals. Due to the complementary and synergistic interaction of functional monomers, high affinity imprinted cavities were formed to recognize TBZ. MIECLS had a wide detection range of 1 × 10 −9 –1 × 10 −5 mol L −1 with the limit of detection of 1.69 × 10 −10 mol L −1 and had huge application potential to detect pesticide residues.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Guang Yang,Fengrui Li,Haoru Zhang,Honglei Yan,Shuang Gao,Ying Fu,Fei Ye
Prochloraz is an extensively used imidazole fungicide in agriculture, but low water solubility limits its application. In the present study, inclusion complex nanofibers of prochloraz and Hydroxypropyl- γ -Cyclodextrin (prochloraz/HP γ CD-IC-NF) were prepared by electrospinning method. The results of various characterization experiments indicated that prochloraz was effectively encapsulated in the cyclodextrin cavity to form inclusion complex nanofibers. Phase solubility study demonstrated that HP γ CD had a significant solubilization effect on prochloraz. In 10 mM HP γ CD solution, the solubility of prochloraz was increased about 2.68 times. Thermogravimetric analysis experiments indicated the beginning thermos-decomposed temperature of prochloraz in prochloraz/HPγCD-IC-NF was raised by 31℃ (from 281℃ to 312℃). The molecular simulation visually showed the best state and the most stable model of prochloraz entering the cyclodextrin cavity. In addition, the fungal inhibitory activity of prochloraz/HP γ CD-IC-NF against Gibberella was 4.559 times to that of prochloraz. In conclusion, the preparation of inclusion nanofibers can improve the water solubility, thermal stability and antifungal activity of mimosine, which has the potential to reduce environmental damage in agricultural applications.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yibing Yan,Fangxiang Song,Songye Li,Tingxian Li,Qian Li,Yan Li
Accurate pesticide application enhances efficiency, reduces usage and minimizes environmental risks. This study employed amino-functionalized degradable mesoporous organosilicon nanoparticles (DMON-N) as a carrier, with chitosan (CS) acting as a capping agent. Chemical cross-linking of CS with Cu 2+ through chelation promotes the formation of Cu 2+ -CS complexes. A pH/redox dual-responsive prochloraz (PRO) delivery system (Cu-CS@PRO-DMON, CCPD) was constructed and evaluated for its potential application in controlling rice sheath blight. The successful preparation of CCPD was confirmed through a series of physicochemical characterizations. The findings demonstrated that CCPD exhibited a high PRO loading capacity (29.09 %) and that PRO could be released from CCPD for an extended duration (up to 144 h) under the influence of acidity and glutathione promotion. Furthermore, CCPD demonstrated excellent wettability (measured contact angle: 63.52 ± 0.42°), adhesion (23.83 ± 2.59 mg/cm 2 ) and resistance to rainfall washout on rice leaves. CCPD demonstrated superior antimicrobial efficacy to prochloraz technical (PRO TC) material and prochloraz emulsion in water (PRO EW). The biosafety assessment revealed that CCPD exhibited minimal acute toxicity to zebrafish, no discernible toxicological effects on human bronchial epithelial cells and no notable impact on rice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 39
·28976-28983
·
IF 3.9
Junqi Feng,Yanyan Zheng,Tao Luo,Fengna Xi,Hao Lai
The development of simple, rapid, and sensitive methods for detecting pesticide in environmental and food samples holds significant importance. Electrochemiluminescence (ECL) sensing platforms with high resistance to interference and contamination, and reduced consumption of ECL emitters, are highly desirable for such applications. In this work, we present an ECL sensing platform based on a graphitic carbon nitride nanosheets (CNNS) supported vertically ordered mesoporous silica film (VMSF) modified glassy carbon electrode (GCE) for the highly sensitive detection of the environmental pollutant 2,4,6-trichlorophenol (TCP) and the broad-spectrum insecticide prochloraz. Two-dimensional (2D) CNNS were synthesized by exfoliating bulk graphitic carbon nitride (g-C3N4) using concentrated sulfuric acid, serving as a novel conductive and adhesive layer for the growth of a stable VMSF on GCE via an electrochemical assistance self-assembly (EASA) method to prepare VMSF/CNNS/GCE. The electrostatic enrichment capability of VMSF nanochannels for the positively charged ECL emitter tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) realized stable and significantly enhanced ECL signals at a low concentration of Ru(bpy)32+ (10 μM). Based on the quenching effect of TCP on the ECL signal of Ru(bpy)32+, highly sensitive ECL detection of TCP was achieved by the VMSF/CNNS/GCE with a linear range from 10 nM to 0.7 mM and a low detection limit (DL) of 2.2 nM. As the metabolic end product of prochloraz is TCP, indirect ECL detection of prochloraz was also accomplished by measuring the produced TCP. Combined with anti-fouling and anti-interference abilities, as well as signal amplification of VMSF, the developed VMSF/CNNS/GCE sensor enabled the sensitive ECL detection of TCP in pond water and prochloraz in orange peel extract.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Xin-Yu Ji,Bing-Yi Wang,Yi-Feng Zhang,Yu-Jing Zhang,Ya-Jie Lai,Yang Yang,Xiang-Chen Wang,Su-Yan Wang,Pedro Laborda,Xin-Chi Shi
BACKGROUND Epicoccum sorghinum is a pathogenic fungus that causes leaf spot in a wide range of plants, including maize, and synthesizes the mycotoxin tenuazonic acid (TEA), which is carcinogenic. Despite the relevant economic and yield losses caused by E. sorghinum worldwide, methods for the control of this pathogen are lacking. RESULTS In this work, the efficacy of Bacillus -produced dipicolinic acid (DPA) for control of E. sorghinum was evaluated using in vitro and in vivo assays, and compared with the efficacy of three commercial fungicides, including carbendazim, prochloraz, and thiram. DPA inhibited E. sorghinum mycelial growth, and conidia germination, and produced important alterations in E. sorghinum hyphae. Interestingly, 10 mM DPA reduced TEA biosynthesis by 86.6%. Although DPA rapidly degraded on maize leaves, 10 mM DPA showed higher preventive (67.4% lesion length inhibition) and inhibitory (89.5% lesion length inhibition) efficacies for the control of E. sorghinum on maize leaves compared to the commercial fungicides. CONCLUSIONS Collectively, this study presents the first method for the control of E. sorghinum on maize and demonstrates that DPA application is a suitable approach to inhibit E. sorghinum symptoms in plants and TEA biosynthesis. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 24
·2022
·
IF 4.4
Qiongmei Mai,Yu Lu,Qianyu Cai,Jianglong Hu,Yunyou Lv,Yonglan Yang,Liqiang Wang,Yuezhao Zhou,Jie Liu
Sclerotinia sclerotiorumis one of the fungi that cause plant diseases. It damages plants by secreting large amounts of oxalic acid and cell wall-degrading enzymes. To meet this challenge, we designed a new pH/enzyme dual-responsive nanopesticide Pro@ZnO@Pectin (PZP). This nanopesticide uses zinc oxide (ZnO) as a carrier of prochloraz (Pro) and is encapsulated with pectin. When encountering oxalic acid released bySclerotinia sclerotiorum, the acidic environment promotes the decomposition of ZnO; at the same time, the pectinase produced bySclerotinia sclerotiorumcan also decompose the outer pectin layer of PZP, thereby promoting the effective release of the active ingredient. Experimental data showed that PZP was able to achieve an efficient release rate of 57.25% and 68.46% when pectinase was added or under acidic conditions, respectively. In addition, in vitro tests showed that the antifungal effect of PZP was comparable to that of the commercial Pro (Pro SC) on the market, and its efficacy was 1.40 times and 1.32 times that of the Pro original drug (Pro TC), respectively. Crucially, the application of PZP significantly alleviated the detrimental impacts of Pro on wheat development. Soil wetting experiments have proved that PZP primarily remained in the soil, thereby decreasing its likelihood of contaminating water sources and reducing potential risks to non-target organisms. Moreover, PZP improved the foliar wettability of Pro, lowering the contact angle to 75.06°. Residue analyses indicated that PZP did not elevate prochloraz residue levels in tomato fruits compared to conventional applications, indicating that the nanopesticide formulation does not lead to excessive pesticide buildup. In summary, the nanopesticide PZP shows great promise for effectively managingSclerotinia sclerotiorumwhile minimizing environmental impact.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Yibing Yan,Songye Li,Shanshan Wang,Yifan Peng,Kaikun Pi,Bin Sun,Liang Cheng,Bing Liu,Fangxiang Song
BACKGROUND Rice sheath blight, caused by Rhizoctonia solani ( R. solani) poses a significant threat to global rice production. Although prochloraz (PRO) is an effective fungicide, its poor photostability and low water solubility severely limit its field performance. We developed a sustainable, stimuli-responsive delivery system to enhance PRO efficacy and minimize environmental risks. RESULTS Multilayered mesoporous nanoparticles (MSN@PMO) were synthesized via a hard-template route and converted into hollow periodic mesoporous organosilicas (H-PMO) by sodium carbonate etching. PRO was encapsulated in H-PMO and sealed with pectin, yielding a dual pH/pectinase responsive formulation (Pec@PRO-H-PMO, PPHP). PPHP exhibited sustained release for 144 h under acidic and enzymatic conditions, and showed markedly improved photostability, foliar adhesion and rainfastness compared with PRO technical concentrate (PRO TC) and PRO water emulsion (PRO EW). Bioassays demonstrated that PPHP exhibited 1.8 times greater antifungal activity against R. solani compared to PRO TC. Ecotoxicological analysis revealed 11.9 times higher zebrafish median lethal concentration (LC₅₀) values than PRO TC over 80% viability of human bronchial epithelial (BEAS-2B) cells at 18.4 mg/L, suggesting improved biosafety. CONCLUSIONS The dual pH/pectinase triggered release of PPHP enhanced pesticide utilization and improved safety. This strategy presents a novel approach for developing stimuli-responsive pesticide delivery systems, aiming to strengthen crop disease control efficiency and mitigate environmental risks. © 2025 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）