FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Yuanhao Zhou,Jiawen Ai,Lingli Cai,Yiyong Yan,BingZhi Wang,Hongzhen Ma,Quan Yu,Jianhua Zhou,Xinming Huo
Excessive pesticide use poses a significant threat to food safety. Rapid on-site detection of multi-target pesticide residues in vegetables is crucial due to their widespread distribution and limited shelf life. In this study, a rapid on-site screening method for pesticide residues on vegetable surfaces was developed by employing a miniature mass spectrometer . A direct pretreatment method involves placing vegetables and elution solution into a customized flexible ziplock bag, allowing thorough mixing, washing, and filtration. This process effectively removes pesticide residues from vegetable surfaces with minimal organic solvent usage and can be completed within 2 min. Moreover, this study introduced a deep learning algorithm based on a one-dimensional convolutional neural network, coupled with a feature database, to autonomously discriminate detection outcomes. By combining full scan MS and tandem MS analysis methods, the proposed method achieved a qualitative recognition accuracy of 99.62%. Following the qualitative discrimination stage, the target pesticide residue and internal standard can be simultaneously isolated and fragmented in the ion trap, thus enabling on-site quantitative analysis and warning. This method achieved a quantitative detection limit of 10 μg/kg for carbendazim in cowpea. These results demonstrate the feasibility of the proposed analytical system and strategy in food safety applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Yexuan Hu,Tongqing Liu,Shuanglei Wang,Meijin Lv,Yongxin She,Bin Cao,Ziping Zhang,Xiaolin Cao
In this study, the novel magnetic molecularly imprinted polymers were successfully synthesized by surface imprinting technique and used as magnetic adsorbent for extracting six strobilurin fungicides from agricultural products. The magnetic molecularly imprinted polymers showed the typical core-shell structure with high maximum adsorption capacity (42.7 mg/g) and rapid adsorption equilibration (3 min) and specific recognition ability. The parameters (elution solvent, elution time, adsorption time, loading solvent and solution pH) in magnetic solid phase extraction were evaluated. Under the optimum adsorption and desorption condition, the magnetic solid phase extraction process based on magnetic molecularly imprinted polymers coupled with high performance liquid chromatography-tandem mass spectrometry was developed for the sensitive detection of strobilurin fungicides from agricultural products. The developed method presented good linearity (5–200 ng/mL) with correlation coefficients (R 2 ) ≥ 0.9990. The limits of detection and quantification were 0.03–0.49 ng/g and 0.09–1.64 ng/g, respectively. At the three different concentration (20, 50, 100 ng/g) spiked in agricultural products, the good recovery (71.5%−115.8%) was obtained and the relative standard deviations were below 8.3%. These results indicated that this established method was sensitive, accurate, selective and reproducible.
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Xuanjun Guo,Ruihua Zhang,Changsheng Li,Manman Duan,Niannian Cao,Qian Jin,Xuejun Chen,Liqin Li,Xuefeng Li,Sen Pang
A widely applied pesticide of azoxystrobin, is increasingly detected in the water environment. Concern has been raised against its potential detriment to aquatic ecosystems. It has been shown that exposure to azoxystrobin interfere with the locomotor behavior of zebrafish larvae. This study aims to investigate whether exposure to environmental levels of azoxystrobin (2 μg/L, 20 μg/L, and 200 μg/L) changes the behavior of male adult zebrafish. Herein, we evaluated behavioral response (locomotor, anxiety-like, and exploratory behaviors), histopathology, biochemical indicators, and gene expression in male adult zebrafish upon azoxystrobin exposure. The study showed that exposure to azoxystrobin for 42 days remarkably increased the locomotor ability of male zebrafish, resulted in anxiety-like behavior, and inhibited exploratory behavior. After treatment with 200 μg/L azoxystrobin, vasodilatation, and congestion were observed in male zebrafish brains. Exposure to 200 μg/L azoxystrobin notably elevated ROS level, MDA concentration, CAT activity, and AChE activity, while inhibiting SOD activity, GPx activity, ACh concentration, and DA concentration in male zebrafish brains. Moreover, the expression levels of genes related to the antioxidant, cholinergic, and dopaminergic systems were significantly changed. This suggests that azoxystrobin may interfere with the homeostasis of neurotransmitters by causing oxidative stress in male zebrafish brains, thus affecting the behavioral response of male zebrafish.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Beibei Zhao,Xinfang Liu,Zheng Cheng,Xu Liu,Xiaoyu Zhang,Xun Feng
Pymetrozine (PYM) is an effective pyridine insecticide for controlling aphids, while its residues pose a serious threat to human health. Herein, a europium complex (Eu-DBPA, DBPA represents deprotonated 2,5-dibromoterephthalic acid ligand) probe was prepared for the detection of PYM via fluorescence quenching . The detection process has the advantages of short response time (2 min), wide linear range (0–4 and 4–45 mg/kg) and low detection limit (2.2 μg/kg). Furthermore, a portable detection platform was designed by integrating Eu-DBPA-based paper strip with smartphone and applied for the visual detection of PYM in real cucumber, tomato, cabbage and apple samples, obtaining satisfactory recovery (99.00 %–107.00 %) and low standard deviation (RSD < 3.4 %). In addition, a logic gate device was designed to simplify the detection process. The smartphone-integrated paper-based probe detection platform provides a new strategy for intelligent and online identification of hazards in environmental and biological samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Yan Wang,Kepin Ni,Zhenyan Zhang,Nuohan Xu,Chaotang Lei,Bingfeng Chen,Qi Zhang,Liwei Sun,Yiling Chen,Tao Lu,Haifeng Qian
The emergence and transmission of antibiotic resistance genes (ARGs) and virulence factors (VFs) pose health risks to the ecosystem and humans. Understanding how non-antibiotic antimicrobial agents drive the expression of ARGs and VFs in freshwater ecosystems, however, remains large challenges. Here, we employed freshwater microcosms and performed metatranscriptomic analysis to investigate the expression profiles of ARGs and VFs in response to pollutants of non-antibiotic antimicrobial agents, including silver nanoparticles (AgNPs) and azoxystrobin. Results showed that AgNPs significantly inhibited the total expression of ARGs and VFs and decreased the number of pathogenic microorganisms expressing these genes. Azoxystrobin increased the total expression of ARGs and VFs, as well as the number of pathogens expressing VFs, but concomitantly reduced the number of pathogens expressing ARGs. Two tested pollutants dramatically changed the expression profiles of ARGs and VFs, with distinct patterns: AgNPs displayed a negative effect, while azoxystrobin showed a positive effect on their expression profiles. Our findings provided a systematical insight to demonstrate that non-antibiotic antimicrobial agents with different mechanisms of action showed various effects on ARGs and VFs, and therefore represented different ecological risks.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xuanjun Guo,Ruihua Zhang,Qian Jin,Niannian Cao,Jingjing Shi,Xingxing Zong,Xuejun Chen,Chen Wang,Xuefeng Li,Sen Pang,Liqin Li
Azoxystrobin, a strobilurin widely used to control rice diseases, has raised concerns about possible adverse effects on aquatic ecosystems. At present, very little is known about the effects of azoxystrobin on courtship and aggressive behavior and the potential underlying mechanisms. In the present study, after exposing adult male and female zebrafish to worst-case scenario concentrations of azoxystrobin (0, 2 μg/L, 20 μg/L, and 200 μg/L) for 42 d, we observed a decrease in courtship behavior and an increase in aggressive behavior in both male and female zebrafish. In addition, to elucidate the molecular mechanism of the behavioral effects of azoxystrobin, we quantified the changes in the concentrations of kisspeptin, 5-HT, GnIH, and their corresponding receptor mRNA expression in the brain. The results showed that 200 μg/L azoxystrobin decreased the concentrations of kisspeptin and increased the concentration of GnIH in both male and female zebrafish brain. In addition, azoxystrobin also significantly reduced 5-HT concentration in female zebrafish brain. Further investigation revealed that altered courtship and aggressive behavior were associated with the expression levels of genes (kiss1, kiss2, gnrh3, gnrhr3, 5ht1a, and 5ht2a) involved in kisspeptin-GnIH signaling pathway. In conclusion, our study suggested that azoxystrobin may impair courtship and aggressive behavior in zebrafish by interfering with the kisspeptin-GnIH signaling pathway, which may have more profound effects on natural zebrafish populations.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Qinghua Zhang,Lei Zhang,Zehua Li,Lixia Zhang,Daping Li
The degradation of fipronil was investigated in microbial fuel cells (MFCs). Almost 79% of 30 mg/L fipronil was rapidly degraded within 12 h by MFC biofilm. Based on the constructed quadratic polynomial model, a maximum fipronil degradation rate of 94.22% could be theoretically achieved at pH of 7.01, 33.39 °C, and the initial fipronil concentration 74 mg/L after incubation for 72 h. The high acute toxicity of fipronil toward zebrafish was largely eliminated after degradation by the MFC. In addition, the MFC biofilm showed catabolic versatility to 4-chloronitrobenzene, sulfanilamide, fluoroglycofen, and azoxystrobin. The microbial community analysis revealed that the functional bacteria Sphaerochaeta , Pseudomonas , Azospirillum , Azoarcus , and Chryseobacterium were major predominant bacteria in the anodic biofilm. Therefore, the MFC offers a promising approach in treating the environmental contaminants due to its abilities of energy capture from waste substances and catabolic versatility to different organic compounds.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 7
·1304
·
IF 3.06
Longzhu Bao,Shuangshuang Wang,Di Song,Jingjing Wang,Xiufang Cao,Shaoyong Ke
A series of novel 3-aryl-4-hydroxy-2(5H) furanone-acrylate hybrids were designed and synthesized based on the natural butenolides and acrylates scaffolds. The structures of the prepared compounds were characterized by1H-NMR,13C-NMR and electrospray ionization mass spectrometry (ESI-MS), and the bioactivity of the target compounds against twelve phytopathogenic fungi was investigated. The preliminary in vitro antifungal activity screening showed that most of the target compounds had moderate inhibition on various pathogenic fungi at the concentration of 100 mg·L−1, and presented broad-spectrum antifungal activities. Further studies also indicated that compounds7eand7kstill showed some inhibitory activity againstPestallozzia theae,Sclerotinia sclerotiorumandGibberella zeaeon rape plants at lower concentrations, which could be optimized as a secondary lead for further research.Keywords:3-aryl-4-hydroxy-2(5H)-furanone;butenolides;acrylate;antifungal activity;natural product
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2017
·
IF 5.099
Tao Lu,Youchao Zhu,Jiahui Xu,Mingjing Ke,Meng Zhang,Chengxia Tan,Zhengwei Fu,Haifeng Qian
The top-selling strobilurin, azoxystrobin (AZ), is a broad-spectrum fungicide that protects against many kinds of pathogenic fungi by preventing their ATP production. The extensive use of AZ can have negative consequences on non-target species and its effects and toxic mechanisms on algae are still poorly understood. In this work, Chlorella pyrenoidosa that had been grown in BG-11 medium was exposed to AZ (0.5–10 mg L −1 ) for 10 d. The physiological and molecular responses of the algae to AZ treatment, including photosynthetic efficiency, lipid peroxidation level, antioxidant enzyme activities , as well as transcriptome-based analysis of gene expression, were examined to investigate the potential toxic mechanism. Results shows that the photosynthetic pigment (per cell) increased slightly after AZ treatments, indicating that the photosystem of C. pyrenoidosa may have been strengthened. Glutathione and ascorbate contents were increased, and antioxidant enzyme activities were induced to relieve oxidative damage (e.g., from lipid peroxidation) in algae after AZ treatment. Transcriptome-based analysis of gene expression combined with physiological verification suggested that the 5 mg L −1 AZ treatment did not inhibit ATP generation in C. pyrenoidosa , but did significantly alter amino acid metabolism, especially in aspartate- and glutamine-related reactions. Moreover, perturbation of ascorbate synthesis, fat acid metabolism, and RNA translation was also observed, suggesting that AZ inhibits algal cell growth through multiple pathways. The identification of AZ-responsive genes in the eukaryotic alga C. pyrenoidosa provides new insight into AZ stress responses in a non-target organism.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2017
·Vol. 74
·期 3
·726-736
·
IF 3.253
Bao-Lei Wang,Hong-Wei Zhu,Zheng-Ming Li,Li-Zhong Wang,Xiao Zhang,Li-Xia Xiong,Hai-Bin Song
BACKGROUND In recent years, pyridylpyrazole derivatives, such as pyridylpyrazole-containing anthranilic diamide have attracted much attention by virtue of their useful insecticidal properties and unique action mode. Moreover, some pyridylpyrazole-containing compounds have also been found to possess significant fungicidal activities. With the aim of discovering new bioactive agrochemicals for crop protection, a series of poly-heterocyclic compounds containing pyridylpyrazole and aziridine, or β-lactam, or thiazolinone moieties were synthesized. RESULTS A series of pyridylpyrazole-containing poly-heterocyclic compounds were obtained, and confirmed through IR, 1 H NMR, 13 C NMR, HRMS and elemental analysis. The crystalline structure of 4-(3-bromo-1-(3-chloropyridin-2-yl)-1 H -pyrazol-5-yl)-3-chloro-1-mesitylazetidin-2-one (compound 13f ) was determined to further illustrate a trans- configuration of the β-lactam motif. In addition, bioassays showed that most of these new compounds exhibited modest insecticidal activity towards Mythimna separate Walker at 200 µg mL −1 . Some of the compounds displayed excellent fungicidal activity towards some plant fungi, including Cercospora arachidicola ( 13j : EC 50 = 14.5 µg mL −1 ), Physalospora piricola ( 12d and 13d : EC 50 = 10.5 and 9.70 µg mL −1 ), Alternaria solani Sorauer ( 13j : EC 50 = 7.29 µg mL −1 ) , Puccinia sorghi Schw. ( 13d : control efficacy 99.0 ± 2.1% at 200 µg mL −1 ) and Erysiphe graminis ( 14d : control efficacy 95.0 ± 1.4% at 200 µg mL −1 ). CONCLUSION Compounds 12b – 12e , 13a , 13d , 13f , 13j , 13 k and 14d could be considered potential fungicidal lead compounds to do further structural optimization. The structure–activity relationship analysis in this study brings some new understanding to the biological activities of N -pyridylpyrazole-containing compounds, and provides important information for the research and development of novel agricultural fungicides with poly-heterocyclic structures. © 2017 Society of Chemical Industry
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Min Wang,Yajie Yue,Xiaoning Wei,Jinyuan Zhang,Xinyuan Bi,Liyan Jia,Xu Jing
Strobilurin fungicides are widely used pesticides globally; however, their residues pose a risk to environmental safety and human health. This study aimed to develop a rapid and green method for detecting strobilurin fungicide residues. Chitosan and magnetic deep eutectic solvent-based emulsive liquid-liquid microextraction method combined with high-performance liquid chromatography was developed to determine strobilurin fungicides in water samples. A high-concentration oil-in-water emulsion containing chitosan solution and extractant was formulated, followed by the addition of samples to form a low-concentration oil-in-water emulsion to complete the rapid pretreatment, therefore solving the limitation of emulsive liquid-liquid microextraction in the inapplicability of extractants. Traditional microextraction required toxic solvents and complex equipment to facilitate the dispersion of extractants, whereas this study used only chitosan solution to aid in emulsifying extractants. The new green magnetic deep eutectic solvent synthesized by tetraoctylammonium bromide, ferric chloride, and oleic acid was used instead of toxic extractants. An external magnetic field assisted the extractant phase collection, which shortened the separation time. Under optimal conditions, the recovery of this method was 83.3 %–107.4 %, the limit of detection was 0.0010–0.0021 mg L −1 , and the coefficient of determination was >0.994. This rapid, green, simple, and efficient chitosan-based microextraction method could detect strobilurin fungicide residues in water samples because of its enhanced compatibility with hydrophobicity extractants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Jin Liu,Wei Pan,Suzhen Li,Yuanjun Nie,Xinyuan Bi,Xu Jing,Li Li
A green solvent-based high-throughput dispersive liquid-liquid microextraction (HTDLLME) method has been developed as an environmentally friendly, straightforward, and efficient sample pretreatment technique. Six batches of extractant (eucalyptol) and dispersant (γ-valerolactone) mixtures were simultaneously injected into six samples and uniformly dispersed using electric 12-channel pipettes, achieving rapid extraction. The extractant and dispersant are sustainable, renewable, and non-toxic, eliminating the need for harmful solvents. The rapid dispersion and spontaneous separation of the extractant do not require auxiliary devices or demulsifiers, aside from the electric 12-channel pipettes. The combined use of electric 12-channel pipettes and deep-well plates increased pretreatment throughput, enabled semi-automatic operation, and improved overall efficiency. UHPLC-MS/MS was selected as the detector to ensure the sensitivity of the proposed method. Under optimal extraction conditions, the linear range was 0.002–0.2 μg L –1 . The limit of detection and limit of quantification were 0.001 μg L –1 and 0.002 μg L –1 , respectively. The intra-day and inter-day relative standard deviations ranged from 2.4 % to 5.5 % and 1.5 % to 5.8 %, respectively. The green solvent-based HTDLLME-UHPLC-MS/MS method achieved rapid detection of SFs in different environmental samples, with extraction recoveries of 81.1 %–105.8 %. Greenness and blueness evaluation tools confirmed the environmental friendliness and practicality of the method. The green solvent-based HTDLLME approach provides a sustainable, straightforward, and practical solution for processing multiple samples simultaneously, supporting effective environmental monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）