RSC Advances
·
2018
·Vol. 8
·期 39
·21850-21856
·
IF 2.936
Wei Xu,Qingzhou Dai,Yuzhi Wang,Xiaojian Hu,Panli Xu,Rui Ni,Jiaojiao Meng
A novel magnetic (Fe3O4) surface molecularly imprinted polymer (MIP) based on ionic liquid (IL) (Fe3O4@VTEO@IL-MIPs) was prepared for the selective extraction of lysozyme (Lys). As the functional monomer of the MIPs, an imidazolium-based IL with vinyl groups was prepared. It can provide multiple interactions with template molecules. The amount of IL was optimized (200 mg). Fourier transform infrared spectrometry (FT-IR), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA) and a vibrating sample magnetometer (VSM) were used to characterize the MIP. The results indicate the successful formation of an imprinting polymer layer. The concentration of Lys in the supernatant was determined by UV-vis spectrophotometry at a wavelength of 280 nm. The maximum adsorption capability of the MIP is 213.7 mg g−1 and the imprinting factor (IF) is 2.02. It took 2.5 h for the MIP to attain adsorption equilibrium. The structure of the protein was evaluated using circular dichroism (CD) spectra and UV-visible spectra. The adsorption performance was further investigated in detail by selective adsorption experiments, competitive rebinding tests, and reusability and stability experiments. Furthermore, it was utilized to separate the template protein from a mixture of proteins and real samples successfully because of the high adsorption capacity for Lys.
研究领域:
分析化学
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材料科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Qing Chen,Yan Xu,Xueting Feng,Yuhan Xiang,Jiayue Ni,Guoyu Ding,Qunxiang Ren,Ming-sheng Zhou
The grafting of a drug molecule, i.e., geldanamycin (GA) onto polyethyleneimine (PEI)-coated magnetic nanoparticle produces a novel composite, GA@Fe 3 O 4 –NH 2 . The composite is confirmed by characterizations with FT-IR, Raman, SEM, EDS, VSM and TEM. Due to the high binding-affinity of GA with myosin heavy chain (MYH), GA@Fe 3 O 4 –NH 2 exhibits excellent adsorption performance towards myosin. Consequently, a solid-phase extraction procedure is established for highly efficient and selective separation of myosin from pig heart extract. At pH 6.0, an adsorption efficiency of 97.1 % is achieved for treating 100 μg mL −1 myosin (0.1 mL) with 0.1 mg GA@Fe 3 O 4 –NH 2 as adsorbent. The adsorption behavior of myosin onto GA@Fe 3 O 4 –NH 2 fits Langmuir model, corresponding to a theoretical adsorption capacity of 518.1 mg g −1 . The adsorbed myosin can be readily recycled by the SDS solution (1 %, m/m) with an elution efficiency of 91.8 %. According to circular dichroism spectroscopy, the conformational changes of myosin during adsorption and elution are reversible. For practical application, myosin is successfully isolated from the pig left ventricular protein extract with GA@Fe 3 O 4 –NH 2 , and SDS-PAGE and LC-MS/MS showed that myosin had high purity and a total of 716 proteins could be identified. Significantly, Geldamycin-encapsulated magnetic nanoparticle for the separation of myosin well exploits the potential of the nanomaterials modified by drug molecules in the separation and purification of target proteins.
研究领域:
蛋白质组学
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纳米技术
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Keliang Wan,Lifeng Tan
Double-stranded RNA determines and regulates many biological processes in cells, plays a vital role in cell information transmission and gene regulation, and is a key target for the treatment of certain diseases. Here, we synthesized and characterized two novel ruthenium(II) complexes [Ru(phen) 2 ( o -CNPIP)] 2+ (Ru 1 ) and [Ru(phen) 2 ( o -CH 3 PIP)] 2+ (Ru 2 , phen = 1,10-phenanthroline, o -CNPIP = (2-(4-cyanodipyrido)-imidazo-[4,5- f ][1,10]-phenanthroline, o -CH 3 PIP = 2-(4-methyl)-imidazo-[4,5- f ][1,10]-phenanthroline). The interaction of the two Ru (II) complexes with double-stranded RNA poly(rA)•poly(rU) under dilute solution and molecular crowding conditions was studied by various spectroscopic methods and viscosity measurements. The results of UV–vis absorption spectroscopy and steady-state emission spectroscopy studies show that Ru 1 showed stronger binding and stabilizing ability to poly(rA)•poly(rU) and the binding affinity of Ru 1 and Ru 2 to poly(rA)•poly(rU) was weakened under molecular crowding conditions. Subsequently, the results of circular dichroism spectroscopy studies, thermal denaturation measurements and viscosity measurements also confirmed the results of our UV–vis absorption spectroscopy and steady-state emission spectroscopy studies. The results also showed that the two Ru(II) complexes bind, stabilize and perturb the structure of poly(rA)•poly(rU) by intercalation under dilute solution and molecular crowding conditions. In addition, it was found that the strength of these abilities was closely related to the binding affinity of the Ru(II) complexes to poly(rA)•poly(rU) by comparing the results of these studies. Furthermore, the thermodynamic parameters for the binding of Ru(II) complexes to poly(rA)•poly(rU) under the two conditions indicate that the binding process is spontaneous, is an enthalpy-driven process, and that hydrogen bonding serves as the dominant force. The purpose of this work is to explore the substituent effect of Ru(II) complexes and the effect of solution environment on the ability of Ru(II) complexes to bind and stabilize poly(rA)•poly(rU). Some insights resulting from this work are expected to facilitate the design of highly selective double-stranded RNA binders based on Ru(II) complexes. Interestingly, the phosphorescence intensity of Ru 2 after binding to poly(rA)•poly(rU) is significantly higher than that of Ru 1 under both solution conditions, with even stronger phosphorescence emission observed under molecular crowding conditions. This indicates that Ru 2 possesses the potential to serve as a nucleic acid luminescent probe for nucleic acid imaging in biological cells. To enhance its luminescent properties and specific binding capacity, further structural modification of Ru 2 is essential, which will be one of the key focuses of our future research efforts.
研究领域:
无机化学
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生物无机化学
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核酸化学
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分子光谱学
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生物物理化学
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DOI
高影响因子
POLYHEDRON
·
2025
·
IF 2.6
Xinying Yuan,Xue Zhang,Qionghua Zhang,Gang Zhao
Three forms of cobalt, including cobalt chloride (CoCl 2 ), cobalt (II) acetylacetonate (Co(acac) 2 ) and cobalt (III) acetylacetonate (Co(acac) 3 ), were evaluated for their interactions with bovine serum albumin (BSA). Results reveal distinct binding mechanisms among cobalt compounds. CoCl 2 bound to negatively charged residues on the BSA surface via electrostatic interactions, exhibiting weak binding affinity ( K b = (0.09–2.3) × 10 3 L·mol −1 ) and high temperature sensitivity (Δ G ° decreased significantly with increasing temperature). Co(acac) 2 partially penetrated the hydrophobic microenvironment of BSA through its hydrophobic ligands, achieving moderate binding strength ( K b = (3.24–6.84) × 10 3 L·mol −1 ) with synergistic contributions from hydrogen bonding and van der Waals forces (Δ H ° = −48.80 ± 0.01 kJ·mol −1 , Δ S ° = −89.87 ± 0.01 J·mol −1 ·K −1 ). Co(acac) 3 demonstrated the strongest binding capacity ( K b = (9.27–32.3) × 10 4 L·mol −1 ), attributed to multimodal interactions (hydrophobic embedding, coordination bonds, and hydrogen bonding) facilitated by its three hydrophobic ligands. The binding affinity of cobalt compounds to BSA increases with ligand complexity and hydrophobicity (CoCl 2 → Co(acac) 2 → Co(acac) 3 ). UV–Vis, three-dimensional fluorescence, and circular dichroism spectra further revealed that organic acetylacetonate ligands enhanced BSA structure perturbation, intensifying with ligand number. Co(acac) 3 induced significant conformational changes in BSA (19.98 % reduction in α -helix content), increased microenvironmental polarity (5 nm fluorescence redshift), and optimized spectral overlap between donor and acceptor (34.6 % increase in J value) by shortening the binding distance ( r = 2.83 ± 0.01 nm), thereby elevating energy transfer efficiency ( E = 34.65 %). This study provides molecular-level insights into the biocompatibility and toxicity assessment of cobalt-based compounds.
研究领域:
无机化学
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生物无机化学
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分子毒理学
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物理化学
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药学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Lei Wang,Dankui Liao,Qian Zhou,Lixia Sun,Xueping Zhang,Xiongdiao Lan,Zhangfa Tong,Meiqun Suo,Guangzhi Zhou
BACKGROUND Food-derived angiotensin-converting enzyme inhibitory peptides (ACEIPs) are commonly utilized in functional food development. In-depth research on their interactions with serum albumin can enhance their bioavailability and in vivo functionality from functional food. RESULTS This study systematically investigated the impact of the ACEIP Gly-Arg-Pro (GRP) derived from sardine muscle protein on the conformation and interaction mechanisms of bovine serum albumin (BSA) under different physiological buffer environments using multispectral techniques, molecular docking theory and isothermal titration calorimetry. GRP demonstrated potent angiotensin-converting enzyme inhibitory activity and had good biosafety in cytotoxicity assays. Steady-state and time-resolved fluorescence spectroscopy indicated the formation of a moderately bound BSA–GRP complex in various buffer environments. The existence of the BSA–GRP complex was further confirmed by ultraviolet–visible spectroscopy. Three-dimensional fluorescence spectroscopy, synchronous fluorescence spectroscopy and circular dichroism spectroscopy demonstrated that adding GRP to BSA affected its microenvironment and structural conformation, with more significant changes observed in the HEPES buffer system due to its distinct physicochemical properties. The thermodynamic parameters indicated that the interaction was mainly driven by hydrophobic forces, making it a spontaneous process regarding Gibbs free energy change. Molecular docking theory showed that GRP is preferentially bound to site II of subdomain IIIA. CONCLUSION This work established a research system for BSA–GRP interactions and obtained relevant parameters, which will benefit the subsequent development of nutritional supplements and functional foods. © 2025 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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分子生物学
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生物物理
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营养学
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药物化学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 190
·期 3
·826-838
·
IF 2.14
Liu Hang,Zhou Min,Ju Xin,Shu Hang,Hu Cuiying,Li Liangzhi
β-Glucosidase (BG) hydrolyzes cellobiose into glucose, and is a vital step in converting ionic liquids (ILs)-pretreated biomass to sustainable biofuels. The inactivation mechanism of BG from Paenibacillus sp. LLZ1 induced by microcrystalline cellulose was explored in various concentrations of ILs, composed of [Emim] + cation and [DEP] − , [OAc] − , [Br] − , [Cl] − , and [BF 4 ] − anions. The FTIR analysis of inactivated BG indicated that the ILs altered its β-sheet content. Moreover, circular dichroism spectroscopy (CD) suggested that the α-helix content decreased, while the β-sheet content increased with the presence of ILs in general. Interestingly, the secondary structure of BG had almost no change after [Emim]DEP treatment, while ionic liquid [Emim]BF 4 treatment caused the irreversible denaturation of BG. Eventually, by adding 0.4 mM of Aerosol OT surfactant, the BG activity was increased by 20.1% in the presence of 25% [Emim]DEP, and the corresponding glucose yield from hydrolysis of cellobiose was increased by 23.9%.
研究领域:
生物化学
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生物技术
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酶学
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可持续能源
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Linlin Wang,Lilong Wang,Chengrong Wen,Nan Wang,Chunhong Yan,Caihong Shen,Shuang Song
Chitosan (CTS)/chitosan oligosaccharide (COS) and whey protein isolate (WPI) have been frequently used as food supplements, but notably, the interaction between the carbohydrate and the protein may affect the digestibility of protein. Thus, the present study focused on effects of the interaction between CTS/COS and WPI on the protein digestibility. A series of chemical and spectroscopic techniques including gel electrophoresis, gel permeation chromatography, Fourier transform-infrared (FT-IR) spectroscopy, intrinsic fluorescence (IF) spectroscopy, and circular dichroism (CD) spectroscopy were applied. According to the findings, both CTS and COS dramatically reduced intestinal digestibility of WPI, resulting in a decrease of DH by 43.33 % and 52.31 %, respectively. The substitution degree of WPI on CTS was 0.87 g WPI/g CTS, and the electrostatic interaction between amine groups of CTS and carboxyl groups of WPI caused changes in WPI's stability, microstructure, and fluorescence intensity. Notably, CTS affected the digestibility of WPI by precipitating protein and enzyme, whereas COS altered WPI's digestibility by decreasing or inactivating enzyme activity. The present study offered a solid scientific foundation for the rational formulations of carbohydrates and proteins in food industry.
研究领域:
食品科学
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营养学
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生物物理学
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化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 41
·30154-30164
·
IF 3.9
Tao Wang,Chuanjiang Ran,Xinyue He,Shengzhou Li,Hongguang Xiang,Yan Shen,Jue Wang,Hongxia Wei
In recent years, with the rapid development of point-of-care testing, the application of lateral flow immunochromatography assay (LFIA) has become increasingly widespread. The key to the success of these detection technologies is the effective binding with diagnostic materials and detection antibody proteins. Although many researchers have tried to optimize antibody binding, a universally accepted strategy that can provide maximum performance has not been determined. In this study, the HIV infection P24 antigen was selected as the detection biomarker. Then the binding mechanism between hollow gold nanoparticles as diagnostic materials and detection antibodies was explored through dynamic light scattering, Fourier transform infrared spectroscopy, circular dichroism spectroscopy, and other methods. It was found that the binding efficiency is related to the change in protein secondary conformation during binding, hydrogen bonding, and van der Waals force maintain the binding mechanism between antibodies and nanoparticles. The main forces of particle complexation and the main binding site of the antibody were discussed and analyzed. Finally, an immunochromatographic system was constructed to evaluate the significant advantages of this platform compared to the common colloidal gold immunochromatographic system.
研究领域:
纳米技术
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免疫学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Lijun Liao,Zhenzi Li,Jing Lu,Yachao Xu,Wei Zhou,Ying Wu
Photocatalytic overall water splitting (POWS), a sustainable route for solar-to-hydrogen conversion, faces challenges from rapid charge recombination and material instability. This study demonstrates a 12-fold enhancement in POWS by integrating spin polarization through platinum quantum dots chemically bonded to brookite/anatase TiO 2 . Advanced characterizations, including X-ray absorption spectroscopy and X-ray photoelectron spectroscopy, confirm Pt-Ti coordination (with a bond length of 2.84 Å) and electron transfer to Pt, thereby enriching the active sites. Magnetic circular dichroism spectroscopy reveals spin-polarized band structures in Pt/TiO 2 , which are absent in pristine TiO 2 , thereby suppressing charge recombination. Under light irradiation, Pt/TiO 2 achieves H 2 and O 2 evolution rates of 310 and 150 µmol h −1 g −1 , respectively, and an apparent quantum yield of 2.1 % at 365 nm. Density functional theory calculations trace this enhancement to optimized hydrogen adsorption free energy and reduced oxygen evolution overpotential. Spin-polarized Ti 3d and Pt 5d orbitals near the Fermi level extend carrier lifetimes, while a shifted d-band center facilitates oxygen desorption. This work establishes spin polarization engineering via cocatalysts as a transformative strategy for POWS, introducing a spin-dependent mechanism that addresses intrinsic limitations in photocatalysis, and offers a pathway to efficient solar fuel generation.
研究领域:
光催化
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材料科学
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物理化学
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纳米技术
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能量转换
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固体物理
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表面科学
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产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2019
·
IF 4.085
Ming Dong,Yujuan Xu,Yumei Zhang,Minyi Han,Peng Wang,Xinglian Xu,Guanghong Zhou
The present study was performed to clarify the effects of pulsed electric field (PEF) on the physicochemical properties and conformations of myofibrillar proteins (MPs) extracted from pale, soft, exudative (PSE)-like chicken breast meat. Various PEF parameters, including the electric field intensity (0–28 kV/cm) and pulse frequency (0–1000 Hz), were varied. The results showed that as the PEF intensity increased, the solubility, surface hydrophobicity and sulfhydryl group content of the MPs were significantly improved. However, when the intensity exceeded 18 kV/cm, these properties declined, which was possibly due to protein aggregation caused by functional group interactions. Additionally, the PEF treatment altered the rheological properties of PSE-like MPs and induced the formation of low-elasticity MPs. However, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that the primary structure of the MPs was not altered after the PEF treatment. According to the circular dichroism (CD) spectroscopy results, the α-helix contents of the PEF-treated samples were increased but the β-turn and random coil contents were reduced. Industrial relevance The occurrence of PSE-like chicken meat is one of the most serious quality issues worldwide and can decrease the consumer's purchasing desire and result in extensive economic losses for the poultry processing industry. MPs play a vital role in the qualitative characteristics of chicken products. As a potential novel processing technology, PEF has potential applications for improving the functionality of PSE-like chicken protein to expand its application in the food industry.
研究领域:
食品科学
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蛋白质化学
·
肉类加工
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产品关联
DOI
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Mengfan Hou,Huan Xiang,Xiao Hu,Shengjun Chen,Yanyan Wu,Jiachao Xu,Xianqing Yang
This study aimed to isolate and identify the novel potential Xanthine oxidase (XOD) inhibitory peptides derived from Trachinotus ovatus hydrolysate (TOH) (hydrolysis by neutral protease), and investigate their structure-function relationship. Initially, TOH was separated to four fractions (F1–F4) by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate (85.16% ± 0.566, 25 mg/mL) and lower molecular weight (95.73%, <1000 Da). F4 was further characterized by reversed-phase high performance liquid chromatography (RP-HPLC) coupled with Nano-HPLC-MS/MS and four peptides (FPAW, LLPW, WLLP and FHLP) were identified with IC 50 value of 3.81 ± 0.18 mM, 4.17 ± 0.12 mM, 43.06 ± 0.73 mM, and over 50.00 mM respectively. It can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD effectively. Moreover, the XOD-inhibiting actions of these peptides involved π-π stacking (Phe914 and Phe1009 in XOD), hydrogen bonding via molecular docking . Lineweaver-Burk equation showed the inhibition type of FPAW was a mixed inhibition. Circular dichroism (CD) spectra indicated FPAW could combine and change the secondary structure of XOD. Therefore, two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.
研究领域:
生物化学
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药理学
·
蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2024
·
IF 6.3
Wujun You,Hongge Liu,Benjamin Ndeshipanda Kashenye,Yinghao Li,Huijuan Zheng,Qiuting Zhang
The research on the application of ultrasound in food processing has been increasing in recent years. In particular, using ice-bath temperature-controlled ultrasound (IBU) to minimize the effects of thermal aspects has been the main focus. However, this approach does not maximize the utilization of energy. Therefore, this study aims to compare the effect of non-temperature-controlled ultrasound (NTU) and temperature-controlled ultrasound (TU), with IBU on the structure and properties of zein. To further explore the role and mechanism of thermal effects in ultrasound processes. Firstly, particle size, zeta potential, hydrophobicity, and thermal stability of zein nanoparticles prepared using various ultrasound-assisted methods were characterized. Compared to the particle size in the IBU group, the NTU group consistently had smaller particles under the same power conditions (e.g., 197 nm versus 176 nm at U1). The particle size in the TU group changes depending on the temperature (e.g., from native 225 nm down to 185 nm). As for the denaturation temperatures, the NTU group (88.9 °C) was significantly higher than that of the IBU (75.76 °C) and TU groups (e.g., 76.9 °C at treatment temperatures of 30 °C). In all, IBU reduces the thermal stability, while NTU treatment enhances it. Furthermore, UV, fluorescence, and CD spectra results indicate that both NTU and TU groups significantly reduce the α-helix structure by unfolding and exposing more internal hydrophobic groups of zein than the IBU group. These results indicate that the effects of ultrasound on zein are closely related to the heating source during the ultrasonic process. IBU could be utilized to maximize the reduction of denaturation levels, but NTU may exhibit superior performance such as protein modification, reducing particle size, or enhancing stability in industrial production.
研究领域:
食品科学
·
材料科学
·
物理化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）