ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Jun-Bo Zhao,Fang Zhang,Yin-Long Guo
To date, direct quantitation of cellular metabolites at the picoliter level or in a single cell is still a challenge due to tiny sampling materials, the accuracy of the sampling volume, and the ubiquitous matrix effect. Herein, picoliter magnitude quantitative analysis was performed using a pressure-assisted microsampling probe coupled to the hydrogen flame desorption ionization mass spectrometer (HFDI–MS). The sampling was accurately controlled with a picoliter pump, and the analytes were rapidly vaporized and quantitatively transferred to the gas phase by adequate heat. The vapor-phase analytes reacted with protonated water cluster ions by the proton-transfer reaction (PTR). The accurate sampling, flash thermal desorption, and proton-transfer ionization processes were conducted spatiotemporally, which could greatly reduce matrix effects to facilitate the quantitation of analytes without the internal standard. Furthermore, this workflow enabled the quantitation of cellular metabolites at the picoliter/single-cell level.
研究领域:
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Yong Wang,Wanjun Gao,Yeyun Li,Yu Xiao,Wei Song,Ting Yao,Manhuan Cheng,Wenjuan Wang,Ruyan Hou
The tea shrub is grown in long-standing orchards, an environment that is suitable for persistent weed growth, which is increasingly controlled by herbicides. Therefore, there is increasing concern that tea consumers may be exposed to herbicide residues. In this study, the levels of glufosinate-ammonium (GLU), glyphosate [N-(phosphonomethyl) glycine; PMG], and its metabolite aminomethyl phosphoric acid (AMPA) were determined in tea samples by HPLC–MS/MS using several current purification methods and a new method that we developed herein. The matrix effect of our proposed method was between -27.3 and 27.7%, which was lower than that in other methods, indicating that this method effectively reduced the interference of tea matrix in the mass spectrometry process. This method was used to determine the levels of PMG, GLU, and AMPA in 780 samples, including six traditional Chinese teas (green tea, black tea, oolong tea, dark tea, white tea, and yellow tea) and a floral tea, from 14 provinces of China. Probability estimates showed that the 95th percentile risk entropy values of the three pesticide residues were far below the acceptable risk level. The risk assessment results showed that exposure to PMG, GLU, and AMPA caused by drinking tea beverages poses no significant risk to human health.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Di Jiao,Zhen-Jie Qian,Yu Wang,Xi Zeng,Yu-Xin Zhong,Lin Luo,Yong-Xin He,Yi-Na Lu,Hong-Tao Lei,Zhen-Lin Xu
Gizzerosine is a biogenic amine in feeds with a physiological toxicity 1,000 times that of its precursor, histamine, causing high mortality in poultry. Because feeds containing gizzerosine usually contain high concentrations of histamine, it is of great significance to establish a method for the simultaneous detection of gizzerosine and histamine for feed safety. Herein, we first developed a detection method using 50 % (v/v) acetonitrile(ACN) containing 1 % (v/v) ammonium hydroxide (AH) extraction to synchronously monitor gizzerosine and histamine in feeds and raw materials via UHPLC–MS/MS. Due to the serious positive matrix effect (ME) in existing LC–MS/MS, this study reduced the ME of samples by purifying the extract solution using a mixed-mode cation exchange based solid phase extraction column (MCX-SPE), and simultaneously obtained accurate quantities of 5–500 ng mL −1 gizzerosine and 25–2,500 ng mL −1 histamine. The limit of detection (LOD) values for gizzerosine and histamine were 1.02 ng mL −1 and 3.00 ng mL −1 , respectively, which were approximately 390 and 8,000 times more sensitive than the maximum recommended dosage of gizzerosine (0.4 mg kg −1 ) and limitation standards of histamine (25 mg kg −1 ) in fish meal, respectively. The samples could only be evaluated in 2 h. The average recoveries of the 6 types of feeds and raw materials ranged from 79.73 % to 110.31 % with a relative standard deviation (RSD) ranging from 1.29 % to 13.35 %. The average recoveries of feeds were generally lower than those of raw materials. Our method was efficient, simple, rapid, accurate, sensitive, and suitable for the simultaneous quantitative analysis of gizzerosine and histamine contents in various feeds, which provided technical support for the quality evaluation of feeds.
研究领域:
动物营养学
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食品科学
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分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Kai Wang,Hui Li,Wenya Liu,Pan Liu,Chuang Jiang,Wanqi Jiang,Yaqing Xiao,Yingnan Liu
Based on the previously developed Al 3+ /CuNCs probe, the matrix effect (ME) derived from protein was investigated in the fluorescence determination of fluoroquinolones (FQs) residues in eggs, and a sample pretreatment method was established to rapidly reduce the ME. Molecular docking results indicated that the three main egg proteins and FQs were bound by hydrogen bond, van der Waals force, and alkyl groups, leading to the loss of FQs response signal, and then produced ME. After trichloroacetic acid treatment, the mean particle size of egg matrix decreased, the protein secondary structure changed, and the crystal structure was destroyed. The corresponding ME of the supernatant obtained by centrifugation was only 0.7 %. The standard addition experiment confirmed that the proposed pretreatment method improved the accuracy and reliability of the fluorescence probe. This work is helpful to promote the practical application of fluorescence analysis in the rapid monitoring of food safety hazard factors.
研究领域:
分析化学
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食品安全
·
荧光光谱学
·
蛋白质化学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2025
·
IF 3
Lin Congcong,Zhu Xuena,Liu Rui,Zhang Li,Yu Tingting,Lu Qun
The consumption of honey contaminated with phytotoxins, particularly from Ranunculaceae plants, is a global public health concern due to their widespread distribution and diverse toxic compounds. This study developed a UPLC-QQQ-MS method to simultaneously quantify 11 phytotoxins of Ranunculaceae family in honey. Analytical (mobile phase) and pre-treatment (extraction solvent and cleanup column) procedures were optimized, identifying Methanol–Water (added 0.1% formic acid and 5 mM ammonium formate), acetonitrile, and SCX column as optimal. Method validation demonstrated negligible matrix effects, good linearity (R 2 > 0.995), good recoveries (81.38–116.85%), and high precision (RSD ≤ 8.63%) for 25–500 ng/mL ranges. LODs and LOQs were 0.02–0.15 µg/kg and 0.07–0.50 µg/kg, respectively (excluding anemonin). Retrospective analysis of 50 honey samples detected magnoflorine, talatisamine, hypaconitine, senecionine, and protopine at positive rates of 2%, 2%, 2%, 8%, and 10%, respectively. Thus, this work provides important technical supports for the risk assessment of phytotoxins in honey.
研究领域:
分析化学
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食品安全
·
毒理学
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植物化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Rui Yang,Yao Lin,Jiahui Yang,Liangbo He,Yunfei Tian,Xiandeng Hou,Chengbin Zheng
A new chemical vapor generation method coupled with headspace solid-phase microextraction miniaturized point discharge optical emission spectrometry (HS-SPME−μPD-OES) for the sensitive and matrix effect-free detection of nitrite in complex samples is described. In an acidic medium, the volatile cyclohexene was generated from cyclamate in the presence of nitrite, which was volatilized to the headspace of the container, efficiently separated, and preconcentrated by HS-SPME. Consequently, the SPME fiber was transferred to a laboratory-constructed thermal desorption chamber wherein the cyclohexene was thermally desorbed and swept into μPD-OES for its sensitive quantification via monitoring the carbon atomic emission line at 193.0 nm. As a result, the quantification of nitrite was accomplished through the determination of cyclohexene. The application of HS-SPME as a sampling technique not only simplifies the experimental setup of μPD-OES but it also preconcentrates and separates cyclohexene from N2 and sample matrices, thus eliminating the interference from water vapor and N2 and significantly improving the analytical performance on the determination of nitrite. Under the optimum experimental conditions, a limit of detection of 0.1 μg L–1 was obtained, which is much better than that obtained by conventional methods. The precision, expressed as relative standard deviation, was better than 3.0% at a concentration of 10 μg L–1. The proposed method provides several advantages of portability, simplicity, high sensitivity, and low energy consumption and eliminates expensive instruments and matrix interference, thus retaining a promising potential for the rapid, sensitive, and field analysis of nitrite in various samples.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Yimei Zeng,Changliang Ke,Qi Liu,Ke Huang
Organophosphate esters (OPEs) are commonly used in commercial products as flame retardants. In the present study, a simple method was established for the simultaneous determination of 10 OPEs in aquacultural water and sediments. The targets were extracted using dichloromethane or ethyl acetate, cleaned up by solid-phase extraction, and determined by gas chromatography – tandem mass spectrometry. The matrix effects were lower than 20% for all OPEs except for tris(2-chloroethyl) phosphate in sediments (matrix effect: 29.4%). In spiked water samples, the recoveries of the OPEs were from 72.3% to 108% with relative standard deviations ≤10.2% and the limits of detection from 0.17 to 1.13 ng·L−1. In spiked sediments, the recoveries of the OPEs were from 101% to 121% with relative standard deviations ≤11.2% with limits of detection from 0.02 to 0.32 ng·g−1, dry weight (dw). The developed method was applied to the analysis of aquaculture samples from Kaozhouyang Bay, China. The total concentration ranges of the 10 OPEs in the water and sediments were from 121 to 159 ng·L−1 and 7.54 to 13.3 ng·g−1, dw, respectively.
研究领域:
环境科学
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分析化学
·
水质
·
环境监测
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 43
·期 17
·3546-3554
·
IF 2.878
Xuemin Ye,Beibei Ye,Jiahui Xu,Mei Fang,Danqi Dong,Congcong Wu,Xupin Lin,Yehui Hu,Xiaoji Cao,Weimin Mo
In this work, a novel quick, easy, cheap, effective, rugged, and safe technique with hydrophobic natural deep eutectic solvent as both extractant and analyte protectant was developed and combined with gas chromatography–tandem mass spectrometry to analyze pyrethroid residues in tomatoes. Eight hydrophobic natural deep eutectic solvents were first evaluated as analyte protectants and those with decanoic acid or lactic acid as hydrogen bond donor were demonstrated to be effective in compensating for the matrix effects of pyrethroids in the gas chromatography system. Hence, they were added to solvent standards for correcting the quantitation errors instead of matrix-matched calibration standards. Then the abilities of these acid-based deep eutectic solvents to extract pyrethriods from tomatoes were evaluated. Results showed the recoveries of all pyrethroids reached to over 80% with only 5 mL menthol:decanoic acid (1:1) used, and good phase separation was easily achieved without the addition of inorganic salt in the extraction step, indicating hydrophobic natural deep eutectic solvent could be a green substitute for acetonitrile in the quick, easy, cheap, effective, rugged, and safe extraction. Compared with the conventional method, the proposed protocol improved the recoveries, reduced the matrix effects, and simplified the extraction step, demonstrating to be an effective, fast, and green method.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
高影响因子
SN Applied Sciences
·
2019
·Vol. 2
·期 1
·1-11
·
IF 0
Li Genrong,Yu Wenqin,Xiao Zhaojing,Long Mei,Tong Lanyan,Qiu Yue
A rapid, sensitive, and practical QuEChERS method coupled with gas chromatography–mass spectrometry was developed for the simultaneous determination of 16 chemical adjuvant residues in samples of fruits (apple, nectarine, grape) and vegetables (tomato, cucumber, spinach). All target compounds were separated in less than 16 min. Samples were extracted with acetonitrile, and the types of extraction salt and cleanup sorbent were optimized. The optimized method was validated by evaluating the linearity, accuracy, precision, and matrix effect. The recoveries of the 16 pesticides adjuvants ranged from 61.2 to 116.3% with relative standard deviations lower than 11.6%. Limits of detection ranged from 0.21 to 3.02 μg/kg, and limits of quantification ranged from 0.71 to 10.07 μg/kg. Finally, the developed method was successfully employed to identify and quantify pesticide adjuvant residues in the analysis of 30 real fruit and vegetable samples.
研究领域:
分析化学
·
食品科学
·
环境科学
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产品关联
DOI
FOOD CHEMISTRY
·
2023
·
IF 8.8
Kai Yao,Jing Zhang,Yumin Niu,Xin Zhang,Yunjia Yang,Yige Wu,Kai Wen,Bing Shao
Bisphenol A (BPA) and its four analogues have been receiving considerable attention owing to their potential endocrine disrupting effects. The European Food Safety Authority has proposed 0.04 ng/kg·body weight/day of the temporary tolerable daily intake for BPA. Therefore, a more sensitive analytical method was urgently needed for the necessity of the risk reassessment of bisphenols (BPs). The matrix effect of Chinese foods is a challenge for the analysis of ultra-trace analytes due to the presence of various spices. A multi-immunoaffinity column (mIAC) was prepared for the purification of BPA, BPB, BPF, BPS, and BPAF in Chinese foods following ultra-high-performance liquid chromatography tandem mass spectrometry detection (UHLPC-MS/MS). The recoveries of each of BPs were ranged from 84.6% to 116.7%, and the intra-day precision and inter-day precision were ranged from 1.6% to 12.4%, and from 4.1% to 14.0%, respectively. This is the first report on the mIACs for simultaneous clean-up and analysis of BPs in complex Chinese foods.
研究领域:
分析化学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 7
·884-895
·
IF 3.1
Xin Wang,Xiaoqi Sun,Yueying Jin,Shengyu Cheng,Yu Han,Minghui Zhang,Lingli Zhang,Xi-Ling Li,Chun-Yan Xu,Jun Zhe Min
This study reports a novel fluorescent chiral derivatization reagent, 4-(N,N-dmethylaminosulfonyl)-2,1,3-benzoxadiazole-(2-succinimidoxy)-trans-2-methyl-L-proline (DBD-S-M-Pro), with a benzoxadiazole structure containing an N-hydroxysuccinimide activation group. DBD-S-M-Pro targets chiral amino-functional compounds under alkaline conditions without a condensation agent. Gradient elution was performed on a BEH C18 (100 × 2.1 mm, 1.7 μm) column with a mobile phase of 0.05% formic acid (FA) in 10 mM ammonium acetate (CH3COONH4) and 0.1% FA in acetonitrile or methanol. The efficiency of the chiral resolution was evaluated under excitation and emission wavelengths of 450 nm and 560 nm, respectively. The 19 chiral amino acids were separated in the range of 1.45–14.84. The resolutions of almost all DL-amino acids exceeded 1.5; the exceptions were serine (Ser) and lysine (Lys), with resolutions of 1.45 and 1.46, respectively. In addition, a new approach was devised for the simultaneous analysis of four chiral amino acids (DL-Glu, DL-Ala, DL-Val, and DL-Phe) in human hair. These amino acids were analyzed in the range of 12.5–400 pmol, with R2 ≥ 0.9990, limits of detection (S/N = 3) of 4–10 pmol, and intraday and interday precisions of 0.57–6.23%. The average spikes in the hair recoveries were 89.76–111.54%, and the matrix effects were 92.47–102.40%. Next, the contents of free chiral amino acids in the hair samples of 10 healthy volunteers (five males and five females) were analyzed with this method, and the differences were compared. The developed DBD-S-M-Pro provides a novel strategy for the sensitive determination of free chiral amino acids in living organisms.
研究领域:
分析化学
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生物化学
·
药学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-17
·
IF 5.3
Chen Mengtian,Yuan Mengdi,Bu Tong,Wang Yang,Huang Xianqing,Song Lianjun,Zhang Pingan,Mao Yexuan,Zhang Xiya
D ifferent samples may cause various types of matrix interference in lateral flow immunoassay (LFA), while deep eutectic solvents (DESs) as emerging green solvents can be synthesized to adapt to different extraction processes by adjusting the composition of hydrogen bond donors (HBDs) and hydrogen bond acceptors (HBAs). Initially, 30 types of food samples were selected to explore the total dilution factors (tDFs) and limit of detection (LODs) on AFB 1 detection by acetonitrile/water extraction. The matrix effect may be induced by some food matrices with high-fat content, including pumpkin seed kernel, almond, pecan, black sesame seed, walnut, and pine nut (tDFs exceeding 70-fold). DES-5 composed of Bet and EG with a molar ratio of 1:3, and a water content of 5% was selected as the optimal extraction solvent, which possessed a strong binding energy with AFB 1 (− 147.35 kJ/mol) but weak interaction with four main fatty acids (− 67.28 kJ/mol to − 74.20 kJ/mol). The DES-based LFA demonstrated that the LODs of pumpkin seed kernel, almond, pecan, black sesame seed, walnut, and pine nut were 0.74 μg/kg, 1.18 μg/kg, 1.01 μg/kg. 0.68 μg/kg, 0.98 μg/kg, and 1.21 μg/kg with recoveries of 79.39% ~ 94.21%, which was about 50% lower than that by acetonitrile/water extraction. According to the results of Soxhlet extraction, 73.46% ~ 85.91% of the total fat content of six food samples with high-fat content was transferred to the upper layer, and the fat content of detection phases (1.16 ~ 3.71 mg/g) by DES extraction (2 mL) was much lower than that of detection phases (4.76 ~ 6.35 mg/g) by acetonitrile/water extraction (2 mL). These results demonstrate that DESs as promising green extraction solvents can weaken the fat-induced matrix effect to achieve accurate detection of AFB 1 , which may provide a new strategy for reducing the interference of other compounds in the use of LFA. Graphical abstract
研究领域:
食品科学
·
分析化学
·
环境科学
·
免疫学
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绿色化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）