MOLECULES
·
2022
·Vol. 27
·期 24
·9039
·
IF 4.927
Yijie Zhang,Zhiwen Jiang,Yanting Wang,Lixin Xia,Shuqin Yu,Hongjian Li,Wei Zhang,Wanshun Liu,Kai Shao,Baoqin Han
Chitosan (CTS) has been used as a nerve guidance conduit (NGC) material for bridging peripheral nerve defects due to its biocompatible, biodegradable, and non-toxic properties. However, the nerve regeneration effect of chitosan alone is restricted due to its inadequate biological activity. Herein, a composite, bioactive chitosan based nerve conduit, consisting of outer warp-knitted tube scaffold made from medical-grade chitosan fiber, and inner porous cross linked carboxymethyl chitosan (C-CM-CTS) sponge with radial texture was developed. The inner wall of the scaffold was coated with C-CM-CTS solution. CM-CTS provided favorable bioactivities in the composite chitosan-based nerve conduit. An in vitro study of CM-CTS revealed its satisfying biocompatibility with fibroblast and its inhibition of oxidative damage to Schwann cells. As the internal filler of the NGC, the lyophilized sponge of C-CM-CTS showed a longitudinal guidance effect for nerve reconstruction. After 10 mm defect in rat sciatic nerve was bridged with the composite bioactive chitosan-based nerve conduit, the nerve conduit was able to effectively promote axonal regeneration and played a positive role in inducing nerve regeneration and functional recovery. In addition to the functional advantages, which are equal to those of an autograft; the technology for the preparation of this conduit can be put into mass production.Keywords:chitosan;carboxymethyl chitosan;nerve conduit;biocompatibility
研究领域:
生物医学工程
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Linlin Wang,Lilong Wang,Nan Wang,Chen Song,Chengrong Wen,Chunhong Yan,Shuang Song
Chitosan (CTS) and chitosan oligosaccharides (COS) have been widely applied in food industry due to their bioactivities and functions. However, CTS and COS with positive charges could interact with proteins, such as whey protein isolate (WPI), influencing their digestion. Interaction among CTS/COS, FUC, and WPI/enzymes was studied by spectroscopy, chromatography, and chemical methods in order to reveal the role of FUC in relieving the inhibition of protein digestibility by CTS/COS and demonstrate the action mechanisms. As shown by the results, the addition of FUC increased degree of hydrolysis (DH) and free protein in the mixture of CTS and WPI to 3.1-fold and 1.8-fold, respectively, while raise DH value and free protein in the mixture of COS and WPI to 6.7-fold and 1.2-fold, respectively. The interaction between amino, carboxyl, sulfate, and hydroxyl groups from carbohydrates and protein could be observed, and notably, FUC could interact with CTS/COS preferentially to prevent CTS/COS from combining with WPI. In addition, the addition of FUC could also relieve the combination of CTS to trypsin, increasing the fluorescence intensity and concentration of trypsin by 83.3 % and 4.8 %, respectively. Thus, the present study demonstrated that FUC could alleviate the inhibitory effect of CTS/COS on protein digestion.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Liang Ma,Xuejuan Shi,Xiaoxiao Zhang,Lili Li
Electrospun core-shell structured nanofibers have got wide attention due to their excellent properties and potential applications. Encapsulated CS in PCL to form the core-shell structured fibers would be a festival way to expanding the applications of biopolymers. In this work, the electrospun core-shell polycaprolacone/chitosan (PCL/CS) composite nanofibers were prepared by a stable emulsion system. Different from other reported works, the surfactant-free and low toxic water-in-oil emulsion was selected to form the electrospinning precursor. The high stability of the emulsion was achieved by adjusting the volume ratio of the solvents and the concentration of the polymers. PCL/CS fibers with different core to shell ratios were obtained via the adjustment of the concentration of CS and PCL in emulsion system. The distribution of the composition in the fibers was analyzed by FT-IR, XRD, EDS, XPS and WCA. Further, intact ultrafine CS fibers with a mean diameter of 143 ± 49 nm could be obtained after the removal of PCL shell, indicating the low diffusivity between core and shell solutions during emulsion electrospinning process. This study provided a promising method to fabricate natural polymer fibers which were difficult to electrospin.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Ziru Lian,Rong Pan,Jiangtao Wang
Norfloxacin chitosan/chitosan oligosaccharide microcapsules (NCCM) were prepared by emulsion-chemical crosslinking method. The characteristics of obtained microcapsules were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and release experiments. Cumulative release profile of norfloxacin from the chitosan microcapsules in natural seawater was measured and the controlled release of drugs was at a uniform rate in 48 h. The chitosan microcapsules were applied onto the antibacterial study of the shrimp culture in natural seawater. It is observed that the seawater in the NCCM added groups was relatively clear and the biomass of Vibrio increased slowly in contrast to the control and norfloxacin groups. The inhibition rate of Vibrio in norfloxacin groups obvioursly decreased after the 5 th day, whereas, it remained high and stable during experiment period in NCCM groups. The results showed that the chitosan microcapsules as release materials have excellent antibacterial effects on Vibrio in the farming of Penaeus vannamei Boone. The controlled release could obviously reduce dosage of antibiotics and delivery times, and effectively improve the utilization rate of norfloxacin drugs for shrimps
研究领域:
水产养殖学
·
药剂学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
KEY ENGINEERING MATERIALS
·
2020
·
IF 0
Yi Peng Wang,Song Yang,Yun Zhen Jia,Pei Jian Sun,Xue Hui Sun,Hong Bo Wang,Jun Wei Guo,Xiao Bing Zhang,Hui Min Liu,Cong Nie
In this study, carboxymethyl peanut shell (CPS) was prepared, and then peanut shell-based porous material (PPS) was obtained by the complexation reaction of CPS with Cu2+. The carboxymethyl content of CPS increased gradually with the increase of the amounts of monochloroacetic acid and sodium hydroxide, and the Cu2+ loading amount of PPS increased gradually with the increase of carboxymethyl content. The scanning electron microscope results showed that PPS-1, PPS-2, PPS-3 (PPS-1~3) had many nanoscale pores. The nitrogen adsorption-desorption results suggested that PPS-1~3 were all mesoporous materials with an average pore diameter of about 10 nm, while their Brunauer-Emmet-Teller surface area and pore volume of PPS-1 to PPS-3 increased gradually. Compared with the control cigarette, the release of HCN in cigarette mainstream smoke could be effectively reduced with the use of PPS-1~3, the reduction rate of HCN per total particulate matter were 54.9%, 63.1% and 67.0%, respectively.
研究领域:
材料科学
·
环境科学
·
烟草研究
✓ 已验证
DOI
FOOD CHEMISTRY
·
2013
·
IF 3.334
Wenjian Li,Hailong Peng,Fangjian Ning,Lihua Yao,Mei Luo,Qiang Zhao,Xuemei Zhu,Hua Xiong
Novel amphiphilic chitosan derivative of N , N -dimethylhexadecyl carboxymethyl chitosan (DCMCs) was synthesised. The physical properties of DCMCs were characterised by Fourier transform-infrared spectroscopy, nuclear magnetic resonance spectroscopy, X-ray diffraction and differential scanning calorimetry. DCMCs can form core–shell micelles with low critical micelle concentration (31 mg L −1 ). The core–shell micelles exhibited spherical shape with positive charge (+50.7 mV) and narrow size distribution (polydispersity index <0.5). Vitamin D 3 (VD 3 ) was initially used to load into the core–shell micelles, and the solubility of VD 3 was improved with higher encapsulation efficiency (53.2%). The in vitro release processes of VD 3 from the core–shell micelles were initially rapid and then followed by a sustained release. The Higuchi model was the most suitable for describing the entire release procedure. DCMCs core–shell micelles are promising carriers for VD 3 and other hydrophobic bioactive food factors.
研究领域:
材料科学
·
食品科学
·
药剂学
✓ 已验证
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·Vol. 142
·期 14
·e56693
·
IF 2.8
Zi-Ang Xia,Long Wang,Chaojie Li,Xue Li,Jingxue Yang,Baoming Xu,Heng Zhang
The augmentation of chitosan's (CS) antioxidative and antibacterial properties via grafting with phenolic acids constitutes a significant method for expanding the utility of chitosan. In this investigation, four phenolic acids (gallic acid, GA; protocatechuic acid, PCA; hydroxybenzoic acid, HA; and salicylic acid, SA) were conjugated with CS utilizing carbodiimide (EDC)-mediated grafting. The resultant phenolic acid-CS graft copolymers underwent characterization employing infrared spectroscopy, x-ray diffraction, and thermogravimetric analysis. Furthermore, ex vivo antioxidative and antibacterial assays were performed. Findings revealed that among the synthesized phenolic acid-chitosan graft copolymers, gallic acid-chitosan (GA-CS) demonstrated the highest antioxidative and antibacterial efficacy, primarily attributed to its elevated grafting ratio and abundance of hydroxyl groups. The antioxidative and antibacterial performances of the remaining three graft copolymers followed this order: CS-PCA > CS-HA > CS-SA, aligning with the trend in grafting ratios. This correlation arises from the higher grafting ratios of copolymers yielding more active OH groups, thereby exhibiting enhanced antioxidative and antibacterial properties. Phenolic acid-chitosan graft polymers exhibit promising antioxidative and antibacterial attributes, making them suitable additives for application in the food preservation packaging industry.
研究领域:
食品科学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Yizhi Li,Shengyue Tang,Ziang Luo,Kun Liu,Yiting Luo,Wei Wen,Shan Ding,Lihua Li,Mingxian Liu,Changren Zhou,Binghong Luo
Natural bone exhibits a complex anisotropic and micro-nano hierarchical structure, more importantly, bone extracellular matrix (ECM) presents liquid crystal (LC) phase and viscoelastic characteristics, providing a unique microenvironment for guiding cell behavior and regulating osteogenesis. However, in bone tissue engineering scaffolds, the construction of bone-like ECM microenvironment with exquisite microstructure is still a great challenge. Here, we developed a novel polysaccharide LC hydrogel supported 3D printed poly( l -lactide) (PLLA) scaffold with bone-like ECM microenvironment and micro-nano aligned structure. First, we prepared a chitin whisker/chitosan polysaccharide LC precursor, and then infuse it into the pores of 3D printed PLLA scaffold, which was previously surface modified with a polydopamine layer. Next, the LC precursor was chemical cross-linked by genipin to form a hydrogel network with bone-like ECM viscoelasticity and LC phase in the scaffold. Subsequently, we performed directional freeze-casting on the composite scaffold to create oriented channels in the LC hydrogel. Finally, we soaked the composite scaffold in phytic acid to further physical cross-link the LC hydrogel through electrostatic interactions and impart antibacterial effects to the scaffold. The resultant biomimetic scaffold displays osteogenic activity, vascularization ability and antibacterial effect, and is expected to be a promising candidate for bone repair.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water
·
2024
·Vol. 16
·期 22
·3304
·
IF 3
Saber A. S. Ahmed,Hongyan Zhai,Soon-Thiam Khu
There is a rising concern about the safety risk that trihalomethanes (THMs) in drinking water pose. In this work, to adsorb THMs such as chloroform (TCM), dibromochloromethane (DBCM), bromodichloromethane (BDCM), and bromoform (TBM), we coated chitosan (CS) on coconut shell activated carbon (CAC). The adsorbents were characterized using BET, XRD, FTIR, and SEM techniques. The impact of various variables was examined, including contact time, quantity of adsorbent, initial pH, and initial THM concentrations. Under the same conditions, TCM was adsorbed most efficiently, followed by BDCM, DBCM, and TBM. When the pH was between 4 and 8, the adsorption of THMs onto the coconut shell activated carbon supported chitosan (CS/CAC) varied relatively little; however, when the pH increased above 8, the adsorption of THMs decreased. For THMs, CS/CAC adsorption was a chemical reaction and monolayer adsorption that fit better with the pseudo-second-order kinetic model and the Langmuir isotherm model. According to the thermodynamic study, THMs were adsorbed endothermically and spontaneously on CS/CAC. For column experiments, the adsorption of THMs was influenced by bed height and flow rate. After up to four cycles of adsorption and desorption, it was found that the adsorbent was reusable. The maximum adsorption capacities for Langmuir were 187.27, 114.29, 93.28, and 89.61 µg/g for TCM, BDCM, DBCM, and TBM, respectively. CS/CAC has a high adsorption capacity, especially for TCM, which is responsible for a major portion of THMs in drinking water. This indicates that CS/CAC has a lot of potential uses when it comes to removing THMs from water.
研究领域:
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Jinyu Li,Dandan Liu,Guoxin Tan,Zhinan Zhao,Xinggang Yang,Weisan Pan
To develop a potential nanocarrier for the topical ocular administration of curcumin (CUR), a novel thiolated chitosan was synthesized by the covalent binding between N -acetyl- l -cysteine (NAC) and chitosan (CS) to surface modify the nanostructured lipid carrier loaded CUR (CUR-NLC). And the superiorities of the CS-NAC co polymer coated CUR-NLC over chitosan oligosaccharides (COS) or carboxymethyl chitosan (CMCS) modification were also verified in detail. As expected, the increment in particle size and the reversal of zeta potential occurred after surface decorating, and the most prominent electropositivity was obtained for the CS-NAC-CUR-NLC group. Additionally, the utilization of the CS-NAC coating demonstrated an effectively controlled release over 72 h and attained a 6.4 and 18.8 fold increase in apparent permeability coefficients (P app ) compared with the CUR-NLC and the self-made eye drops, respectively. Meanwhile, the clearance rate of the NLC labeled with Rhodamine B was significantly delayed in the presence of CS-NAC. By contrast, CS-NAC-CUR-NLC was superior to the COS and CMCS coated ones in view of in vitro release, drug permeability and corneal retention. Moreover, the results of the in-vivo and in-vitro characteristics demonstrated that the promoting effect of CMCS coating was relatively weaker than COS coated ones. Ocular irritation test was executed on the CS-NAC-CUR-NLC, neither a sign of toxicity nor irritation to the external ocular tissues was observed. In conclusion, CS-NAC-CUR-NLC possesses a greater potential as an ocular drug-delivery system comparing with the COS-CUR-NLC and CMCS-CUR-NLC.
研究领域:
药剂学
·
生物材料
·
眼科
✓ 已验证
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 2
·452
·
IF 4.412
Wei-Yan Quan,Song-Zhi Kong,Si-Dong Li,Hua-Zhong Liu,Qian-Qian Ouyang,Yong-Mei Huang,Hui Luo
Chitosan is the only cationic polysaccharide found in nature. It has broad application prospects in biomaterials, but its application is limited due to its poor solubility in water. A novel chitosan derivative was synthesized by amidation of chitosan with 18β-glycyrrhetinic acid and sialic acid. The chitosan derivatives were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and measurement of the zeta potential. We also investigated the solubility, cytotoxicity, and blood compatibility of chitosan derivatives. 18β-glycyrrhetinic acid and sialic acid could be grafted onto chitosan molecular chains. The thermal stability of the synthesized chitosan derivatives was decreased and the surface was positively charged in water and phosphate-buffered saline. After chitosan had been modified by 18 β-glycyrrhetinic acid and sialic acid, the solubility of chitosan was improved greatly in water and phosphate-buffered saline, and percent hemolysis was <5%. Novel amphiphilic chitosan derivatives could be suitable polymers for biomedical purposes.Keywords:chitosan;chitosan derivatives;18β-glycyrrhetinic acid;sialic acid;cytotoxicity
研究领域:
生物材料学
·
医学化学
✓ 已验证
DOI
PubMed
高影响因子
FUEL
·
2024
·
IF 6.7
Mengjie Zhang,Xiangpu Jiao,Dezhang Ren,Chenxin Huang,Yang Li,Duofu Chen,Zhibao Huo
The rapid development of the biodiesel industry is of significant importance for achieving carbon emission reduction in the transportation sector. However, the presence of trace amounts of water in the feedstock during biodiesel synthesis may lead to severe saponification reactions, thereby reducing the yield of fatty acid methyl esters. In this study, based on our exploration of the characteristic of porous carbon to absorb moisture from the environment, we synthesized a CaO catalyst with porous carbon coating via discarded shrimp shell biomass. Because the carbonization temperature of organic matter in shrimp shells is lower than the decomposition temperature of CaCO 3 , the CO 2 generated during pyrolysis can erode the pyrolytic carbon and form porous structure. The mesoporous carbon shell facilitates the diffusion of oil and methanol molecules while adsorbing moisture in the alcohol phase. This structure effectively minimizes the interaction between CaO and water, thereby suppressing saponification reactions and ensuring the smooth progression of the desired transesterification reaction. Through a series of physicochemical characterizations, the structure and water absorption performance of the catalyst were confirmed. The as-prepared CaO@PC-900 catalyst demonstrated the ability to maintain a biodiesel yield of over 85% in a reaction system with a water content of 3%, significantly surpassing the performance of commercial CaO and previously reported Ca-based solid bases. This work provides a new approach to the design of anti-saponification transesterification catalysts, while also offering a novel pathway for the synthesis of porous carbon from discarded crustacean biomass.
研究领域:
能源科学
·
催化材料
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）