Journal of Integrative Agriculture
·
2024
·
IF 4.6
Zhen Liu,Ning Xu,Jumei Hou,Tong Liu
The nascent polypeptide-associated complex (NAC) is involved in various biological functions in eukaryotes which have been extensively studied in animals and plants, but its role in the biocontrol action of microorganisms needs to be better understood. In this study, we investigated the function of TbNACα , one of the subunits of NAC, in the biocontrol activity of Trichoderma breve T069 against Sclerotium rolfsii . The TbNACα gene was deleted from T. breve T069, and the Δ TbNACα mutant showed significantly reduced mycelial growth, spore production, and spore germination. Additionally, volatile substances from Δ TbNACα had no significant effect on S. rolfsii , while non-volatile substances significantly inhibited the growth of S. rolfsii . Transcriptome sequencing results showed that compared to wild-type T069, the Δ TbNACα mutant had 3,398 differentially expressed genes, mainly regulating the expression of genes related to secondary metabolite biosynthetic enzymes, hydrolases, and membrane transport proteins. Untargeted metabolomics identified 50 upregulated metabolites (27 in positive ion mode and 23 in negative ion mode) in crude extracts from Δ TbNACα mutant metabolite broth. Moreover, the metabolic substances of ethyl caffeate had the strongest activity against S. rolfsii , with an EC 50 of 107.15 μg·mL -1 . Quantitative Real-time PCR (qPCR) analysis revealed that genes involved in the ethyl caffeate synthesis pathway were significantly upregulated in Δ TbNACα strains. This study provides a basis for the negative regulation of ethyl caffeate synthesis and elucidates the antagonistic inhibition mechanism of TbNACα in T. breve T069.
研究领域:
微生物学
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分子生物学
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植物病理学
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生物化学
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基因组学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Yunling Zhang,Kun Gao,Zhi Dang,Weilin Huang,John R. Reinfelder,Yuan Ren
Arsenic–rich schwertmannite may cause arsenic (As) release during phase transition. In this study, microbial sulfidogenesis on As(V)–loaded schwertmannite (As-Sch) and associated As mobility at different SO 4 2− concentrations were investigated under anaerobic conditions by Desulfosporosinus meridiei ( D. meridiei ). For biotic treatments, the more SO 4 2− was added, the more Fe 3+ was reduced to Fe 2+ , and the more As(V) was released during the reductive dissolution of As-Sch. The reduction of As(V) to As(III) by D. meridiei resulted in a higher concentration, toxicity, solubility and mobility of As than the corresponding abiotic treatments. However, compared with the abiotic treatments, a variety of new minerals (such as mackinawite , vivianite , sulfur, As 2 S 3 , and parasymplesite) were generated in the biotic treatments, and the As concentration in aqueous solution was less than 1 μM at the end of the incubation period regardless of the presence of SO 4 2− . The results of continuous extraction of different species of As from secondary minerals showed that the effect of microorganisms decreased As content of amorphous iron oxide-bound phase, while increasing that bound on the surface of iron oxide surface-bound phase, thus increasing As fluidity. Our findings indicated that under anaerobic conditions, D. meridiei sulfidogenesis can trigger significant As mobilization in the early stage and remove As from the aqueous solutions when new minerals are formed at a later stage.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Medicine-JIM
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2021
·
IF 3.034
Yue-jiao Shi,Hua-xian Zheng,Zhu-ping Hong,Han-bo Wang,Ying Wang,Ming-yan Li,Zhen-Hao Li
Objective Ganoderma lucidum spore (GLS) is gaining recognition as a medicinal part of G. lucidum and has been reported to possess various pharmacological properties, such as antitumor activity . In this work, wall-broken GLS powder (BGLSP) and wall-removed GLS powder (RGLSP), two kinds of GLS powder with different manufacturing techniques, were compared in terms of contents of active constituents and in vivo and in vitro antitumor effects. Methods The ultraviolet and visible spectrophotometry method was used to determine the contents of polysaccharides and total triterpenoids in BGLSP and RGLSP. Seventeen individual triterpenoids were further quantified using ultra-high-performance liquid chromatography and quantitative analysis of multi-components by single marker. The antitumor effects of BGLSP and RGLSP were evaluated using in vitro cell viability assay against human gastric carcinoma SGC-7901, lung carcinoma A549 and lymphoma Ramos and further validated by in vivo zebrafish xenograft models with transplanted SGC-7901, A549 and Ramos. Results The results showed that the contents of polysaccharides, total triterpenoids and individual triterpenoids of RGLSP were significantly higher than those of BGLSP. Although both BGLSP and RGLSP inhibited the three tumor cell lines in vitro in a dose-dependent manner, the inhibitory effects of RGLSP were much better than those of BGLSP. In the in vivo zebrafish assay, RGLSP exhibited more potent inhibitory activities against tumors transplanted into the zebrafish compared with BGLSP, and the inhibition rates of RGLSP reached approximately 78%, 31% and 83% on SGC-7901, A549 and Ramos, respectively. Conclusion The results indicated that the antitumor effects of GLS were positively correlated with the contents of the polysaccharides and triterpenoids and demonstrated that the wall-removing manufacturing technique could significantly improve the levels of active constituents, and thereby enhance the antitumor activity.
研究领域:
药理学
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肿瘤学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2025
·
IF 5.2
Qing Wang,Rongxue Sun,Ning Jiang,Haojie Ren,Haibo Luo,Cheng Wang,Qianyuan Liu,Zhiqiang Li,Xingna Wang
Bacterial spores in food exhibit strong resistance to conventional processing methods, posing a significant challenge to food safety. This scientific aim of the study was to compare the antimicrobial effects of organic acids and plasma-activated lactic acid (PALA) on the specific spoilage bacteria (SSB) spores in cooked crayfish ( Procambarus clarkii ), and further explored the antimicrobial mechanism of PALA against Bacillus cereus spores. Plasma activation enhanced the antimicrobial efficacy of lactic acid (LA), as 1 % PALA showed equivalent antimicrobial efficacy to 1.5 % LA against spores. Microstructural changes and leakage of core contents indicated that PALA treatment caused damage to the inner membrane (IM) of B. cereus spores. Then, the spore germination experiments further revealed that PALA treatment disrupted the function of SpoVA proteins in the IM, leading to a significant decrease in spore germination capacity. Furthermore, inactivated spores did not regain viability after treatment with lysozyme or Ca-DPA, and no mutants were detected among the surviving spores. These results demonstrated that the inactivation effect of PALA on B. cereus spores is closely related to the structural disruption and dysfunction of the spore IM. Additionally, PALA treatment extended the shelf life and maintained the product quality of cooked crayfish, supporting PALA as a promising measure for controlling microbial safety.
研究领域:
食品微生物学
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非热加工技术
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等离子体技术
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食品安全
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孢子生物学
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 2
·434
·
IF 4.2
Lanwen Zhang,Yongxia Li,Xiaojian Wen,Xuan Wang,Wei Zhang,Dongzhen Li,Yuqian Feng,Zhenkai Liu,Xingyao Zhang
Pine wilt disease (PWD), caused by the pine wood nematode (PWN,Bursaphelenchus xylophilus), is one of the most serious threats to pine forests worldwide. The fungusEsteya vermicola, with its lunate conidia capable of parasitizing the PWN, has shown promise as an efficient biological control agent against PWD. Solid-state fermentation (SSF) is preferred for large-scale applications in the field, as it facilitates microbial agent transport and ensures a long shelf life. However, research on enhancing the yield of lunate conidia fromE. vermicolathrough SSF is limited. In this study, we initially achieved a yield of 3.04 × 108conidia/g using a basic SSF medium composed of wheat bran, corn flour, and soybean flour. To improve this yield, we employed an orthogonal experimental design (OED) to identify the optimal medium composition, which required a wheat bran-to-corn flour-to soybean flour ratio of 7:2:1 (w/w/w), a substrate-to-water ratio of 1:0.7 (w/v), and the addition of 1.33% (w/w) glucose, 1.33% (w/w) yeast extract fermentation, and 1.33% (w/w) MgSO4. Using the response surface methodology (RSM), we calculated the optimal fermentation conditions, which were 24.9 °C, 78.0% relative humidity (RH), an inoculation volume of 16.3% (v/w), and a fermentation time of 7.1 days. Under these conditions, the yield of lunate conidia reached a maximum of 16.58 × 108conidia/g, a 4.45-fold increase after optimization. This study improved the yield ofE. vermicolalunate conidia and provides insights for developing biopesticides based on this strain.
研究领域:
植物病理学
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微生物学
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林业科学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
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IF 7.632
Fei Peng,Bingjie Zheng,Yao Zhang,Aroosha Faheem,Yanyan Chai,Tao Jiang,Xuguang Chen,Yonggang Hu
Enzyme-based catalyzed reactions are of increasing importance in organic synthesis as they are eco-friendly and allow a multitude of selective and efficient reactions in aqueous system. In this work, we used Bacillus amyloliquefaciens spores instead of laccase for organic synthesis because the former had plenty of CotA-laccase on their surface. The yields of spore catalyzed cross-coupling of substituted aromatic amines such as 1,2-phenylenediamine and 2-aminophenol to corresponding phenazine and phenoxazinone reached 70% and 85%, respectively. By coupling with TEMPO, a spore–TEMPO system was developed for the oxidation of primary benzylic alcohols such as benzyl alcohol, 4-methoxybenzyl alcohol, and 3,4-dimethoxybenzyl alcohol to corresponding benzaldehydes with excellent selectivity (99%, 98%, and 98%, respectively) and good yields (approximately 91%, 95%, and 96%, respectively). The developed spore-based systems show several advantages including eco-friendliness, ease of operation and storage, low cost, recyclability, sustainability, and no need for enzyme purification or immobilization. These findings may have promising implications for developing a new eco-friendly and cost-effective technology for organic synthesis.
研究领域:
有机化学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
CHEMICAL PAPERS
·
2019
·Vol. 73
·期 7
·1753-1759
·
IF 1.246
Xu Li,Zhang Ya-Nan,Ji Xing-Hu,He Zhi-Ke
Silicon nanodots (SiNDs) as an excellent fluorescent nanomaterial have got more and more applications. In this work, the terbium-modified silicon nanodots (SiNDs-Tb) were synthesized and successfully applied to the detection of dipicolinic acid (DPA), a biomarker of anthrax spores. Here, SiNDs serve as scaffolds and a fluorescence reference ( F 445 ), coordinating with Tb 3+ ions, while terbium ions as specific recognition elements and response signals ( F 490 and F 547 ) with zero background fluorescence. The sensing process was easily operated in aqueous media and can be finished in a short time. With a concentration range of 0.2–10 μM of DPA, the fluorescence intensity ratio ( F 547 / F 445 ) correspondingly generated quantitative changes and the limit of detection was 24 nM. It is a rapid and sensitive sensing system and provides a new approach for full exploitation of SiNDs in fluorescence assay.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
Gut Microbes
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2022
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IF 9.434
Ningning Xu,Liyuan Lin,Yahui Du,Huibin Lin,Jia Song,Chaoyong Yang,Wei Wang
Transmission of bacterial endospores between the environment and people and the following germination in vivo play critical roles in both the deadly infections of some bacterial pathogens and the stabilization of the commensal microbiotas in humans. Our knowledge about the germination process of different bacteria in the mammalian gut, however, is still very limited due to the lack of suitable tools to visually monitor this process. We proposed a two-step labeling strategy that can image and quantify the endospores’ germination in the recipient’s intestines. Endospores collected from donor’s gut microbiota were first labeled with fluorescein isothiocyanate and transplanted to mice via gavage. The recipient mice were then administered with Cyanine5-tagged D-amino acid to label all the viable bacteria, including the germinated endospores, in their intestines in situ. The germinated donor endospores could be distinguished by presenting two types of fluorescent signals simultaneously. The integrative use of cell-sorting, 16S rDNA sequencing, and fluorescence in situ hybridization (FISH) staining of the two-colored bacteria unveiled the taxonomic information of the donor endospores that germinated in the recipient’s gut. Using this strategy, we investigated effects of different germinants and pre-treatment interventions on their germination, and found that germination of different commensal bacterial genera was distinctly affected by various types of germinants. This two-color labeling strategy shows its potential as a versatile tool for visually monitoring endospore germination in the hosts and screening for new interventions to improve endospore-based therapeutics.
研究领域:
微生物学
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分子生物学
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医学
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-18
·
IF 12.6
Niu Mengya,Zhao Luo,Gong Shuang,Liu Xinxin,Zheng Cuixia,Jiao Jiannan,Wang Fangfang,Wang Lei
Salmonella Typhimurium ( S . Tm), a Gram-negative pathogenic bacterium, is one of the most common causes of invasive bacterial diseases. Antibiotic therapy remains the principal therapeutic modality for treating S . Tm infection. However, due to the difficulty in precisely targeted pathogenic bacteria after oral administration, the therapeutic effect remains unsatisfactory. Here, we developed an oral probiotic spores-based biohybrid delivery system (BCs@PME-Au) to treat S. Tm-induced colitis. By employing a one-pot metal deposition method, Polymyxin E (PME) acted as a reducing agent to promote the Au 3+ rapid nucleation and growth into PME-capped Au NPs (PME-Au NPs). By forming Au-S and Au-N bonds with the active sites (-SH, -NH 2 ) of Bacillus coagulans spores (BCs), PME-Au NPs were anchored onto the surface of BCs to construct the biohybrid system BCs@PME-Au. Following oral administration, BCs@PME-Au successfully passed through the gastric acid barrier. After absorbed water and nutrients, BCs germinated into Bacillus coagulans (BC) in the gut and PME-Au NPs were released. Based on the BC’s targeting pathogen infection site and PME-Au NPs’ targeting Gram-negative bacteria, the biohybrid system achieved significantly antibacterial effect of S . Tm. Mechanistically, by blocked the LPS-induced inflammatory pathway TLR4/MyD88/NF-κB, BCs@PME-Au exerted a powerful anti-inflammatory effect. With its robust antibacterial efficacy, targeted delivery, and excellent safety profile demonstrated both in vitro and in vivo, the biohybrid system BCs@PME-Au offers significant promise in treating bacterial colitis.
研究领域:
微生物学
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医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2024
·
IF 5
Hao Ding,Tiecheng Wang,Yuxiang Zhang,Chunfeng Guo,Kaiyu Shi,Ivan Kurtovic,Yahong Yuan,Tianli Yue
As spores of Alicyclobacillus acidoterrestris can survive traditional pasteurization , this organism has been suggested as a target bacterium in the fruit juice industry . This study aimed to investigate the inactivation effect of cold plasma on A. acidoterrestris spores and the mechanism behind the inactivation. The inactivation effect was detected by the plate count method and described by kinetic models. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), the detection of dipicolinic acid (DPA) release and heat resistance detection, the detection and scavenging experiment of reactive species, and cryo-scanning electron microscopy were used to explore the mechanism of cold plasma inactivation of A. acidoterrestris . The results showed that cold plasma can effectively inactivate A. acidoterrestris spores in saline with a 3.0 ± 0.3 and 4.4 ± 0.8 log reduction in CFU/mL, for 9 and 18 min, respectively. The higher the voltage and the longer the treatment time, the stronger the overall inactivation effect. However, a lower gas flow rate may increase the probability of spore contact with reactive species, resulting in better inactivation results. The biphasic model fits the survival curves better than the Weibull model. SEM and TEM revealed that cold plasma treatment can cause varying degrees of damage to the morphology and structure of A. acidoterrestris spores, with at least 50 % sustaining severe morphological and structural damage. The DPA release and heat resistance detection showed that A. acidoterrestris spores did not germinate but died directly during the cold plasma treatment. 1 O 2 plays the most important role in the inactivation, while O 3 , H 2 O 2 and NO 3 − may also be responsible for inactivation. Cold plasma treatment for 1 min reduced A. acidoterrestris spores in apple juice by 0.4 ± 0.0 log, comparable to a 12-min heat treatment at 95 °C. However, as the treatment time increased, the survival curve exhibited a significant tailing phenomenon, which was most likely caused by the various compounds in apple juice that can react with reactive species and exert a physical shielding effect on spores. Higher input power and higher gas flow rate resulted in more complete inactivation of A. acidoterrestris spores in apple juice. What's more, the high inactivation efficiency in saline indicates the cold plasma device provides a promising alternative for controlling A. acidoterrestris spores during apple washing. Overall, our study provides adequate data support and a theoretical basis for using cold plasma to inactivate A. acidoterrestris spores in the food industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qing-Xuan Ren,Rui Wang,Qin-Ru Mu,Ling Chen,Min Chen,Li-Jiang Wang,Ping Li,Hua Yang,Wen Gao
Ganoderma lucidum spore oil (GLSO) is a dietary supplement, with glycerides (GLs) recognized as its important active component. However, comprehensive profiling and accurate structural characterization of GLs in GLSO remain underexplored. In this study, 59 GLs from GLSO were identified by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) and molecular networking (MN). The double bond isomers of these compounds were further resolved by the Paterno-Büchi (PB) reaction coupled with UPLC-Q-TOF MS, resulting in the identification of 36 unsaturated GLs, including 5 pairs of positional isomers. In summary, 64 GLs were characterized, consisting of 9 diacylglycerols (DGs) and 55 triacylglycerols (TGs). Additionally, the compositional variations, antioxidant activities, and relative isomer ratios of C C positional isomers of GLSO from eight different manufacturers were revealed, with 11 GLs correlating with antioxidant activity. This study enhances the understanding of the nutritional value of GLSO and lays a foundation for future quality standard formulation.
研究领域:
食品科学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Ning Liao,Juan Wang,Guanwen Liu,Yinghui Li,Fengqin Xu,Keyi Xu,Dingyu Shi,Dongyan Shao,Chunmei Jiang,Junling Shi
Inflammatory bowel disease (IBD) is a chronic inflammation with a high incidence rate. Many probiotics, including Lacticaseibacillus rhamnosus (L. rhamnosus), have shown promise in IBD treatment. The therapeutic effects of most probiotics are greatly decided by the available live cells in the disease lesion, which is compromised as they pass through the gastric juice and intestinal tract, resulting in a loss of activity. To improve probiotic delivery efficiency in the intestinal tract, broken Ganoderma lucidum spore shells (bGLS) were explored as a carrier to enhance the intestinal tract delivery of L. rhamnosus SHA113, a probiotic that has been verified to have capability to treat IBD. It was found the bGLS treated with iturin A and hydrochloric acid (IH-bGLS) had much higher affinity to probiotic cells than the untreated ones. This is possibly due to the enhancement of hydrophobic and positive charge of bGLS. Furthermore, IH-bGLS demonstrated an 81% loading efficiency for L. rhamnosus SHA113 and 2.2% for Escherichia coli. More importantly, loading in IH-bGLS greatly enhanced the delivery of L. rhamnosus SHA113 cells to the colon and prolonged their retention time from 48 to over 120 h (P < 0.01). The mechanisms might be related to the enhancement of probiotic cell adhesion to the gastrointestinal mucosa, increase of mucus secretion and the upregulated expression of tight junction proteins, occludin and ZO-1, in the colon. The results of the animal experiment showed that the therapeutic effects of L. rhamnosus SHA113 on IBD were greatly enhanced when they were loaded with IH-bGLS. The novelty of this research is in the development of probiotic carriers from bGLS, which has significance in the improvement of intestinal delivery efficiency and the therapeutic effects of probiotics on IBD. This system may have attractive application in the enhancement of probiotic delivery efficiency in the intestinal tract, which is important to ensure and enhance the beneficial effects of probiotics.
研究领域:
生物工程
·
医学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）