JOURNAL OF APPLIED PHYCOLOGY
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2023
·Vol. 35
·期 5
·2219-2239
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IF 3.3
Gan Tingting,Yin Gaofang,Zhao Nanjing,Tan Xiaoxuan,Wang Ying,Sheng Ruoyu,Ye Ziqi
Microalgal fluorescence kinetics method, as a promising technique for rapidly detecting water toxicity, still lacks a consistent response index with sensitive response characteristics to different types of toxic pollutants. In order to solve this problem, in this study, the influences of six typical toxic pollutants belonging to different types, including the heavy metal cadmium, the herbicide atrazine, organic chemical raw materials 1,4-benzoquinone and 1,4-dichlorobenzene, and the disinfection by-products chloroacetic acid and trichloroacetonitrile on the fluorescence rise kinetics (OJIP) curve of microalga Chlorella pyrenoidosa were investigated. According to the same characteristics of changes in the OJIP curves of different toxic pollutant treatments, a new response index for detecting the comprehensive toxicity of water (RI ct ) was constructed. By comparing with the maximum photochemical quantum yield of photosystem II (PSII) (F v /F m ), the potential quantum yield of PSII (F v /F o ), the performance index of PSII (PI ABS ) and the quantum yield of electron transport of PSII (φ Eo ), the feasibility and sensitivity of quantitative response of RI ct to the toxicity of each pollutant and polluted water containing multiple pollutants were verified. The results indicated that all the six toxic pollutants had the same influence on parameters F v(m-j) /F o and F v(m–o) /F o contained in the OJIP curve, and RI ct constructed based on F v(m-j) /F o and F v(m–o) /F o had a good logistic curve concentration–response relationship with each pollutant. By comparison, the response sensitivity of RI ct to all pollutants was significantly superior to F v /F m , F v /F o and φ Eo , and also had better stability in the toxicity response to each pollutant compared to PI ABS , indicating that RI ct was a suitable response index for rapidly and sensitively detecting water comprehensive toxicity based on microalgal fluorescence kinetics. This study provides an important basis for the development of rapid and sensitive detection methods for water toxicity.
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TALANTA
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2020
·
IF 5.339
Xue Li,Yuan-Yuan Cui,Cheng-Xiong Yang
Microporous organic networks (MONs) have shown great promise in separation science recently. Exploring novel, simple and convenient strategy to fabricate MONs coated capillary columns for gas chromatography (GC) still remains challenging but desirable for the development of MONs in chromatographic separation. To extend the potential application of MONs in separation science and to further develop novel method for the fabrication of MONs-based capillary columns, here we demonstrate a novel covalent coupling strategy to fabricate uniform MONs bonded capillary columns for GC separation of position isomers and hydrocarbons. The bare capillary column was firstly modified with (3-bromopropyl)trimethoxysilane to provide bromine sites for coupling with alkynyl monomers . The amino- and hydroxyl-functionalized MONs (MON-NH 2 and MON-OH) were then directly grown onto the inner wall of the brominated capillary columns via the covalent coupling between bromine and alkynyl groups. The uniform MON-NH 2 and MON-OH bonded capillary columns were obtained and showed good resolution for GC separation of dichlorobenzene , chlorotoluene, bromotoluene, and propylbenzene position isomers and many other hydrocarbons including linear alkanes, alkylbenzenes , pinene isomers, cyclohexane and benzene, ketones and aldehydes. The MONs bonded capillary columns also owned good lifetime and precision for dichlorobenzene isomers with the relative standard deviations (RSDs) of 0.2–0.3%, 1.2–2.1%, and 1.7–2.5% for retention time, peak height and peak area, respectively. In addition, the fabricated MON-NH 2 and MON-OH bonded capillary columns offered better resolution than commercial InertCap-1, InertCap-5, InertCap-1701 and InertCap-WAX capillary columns for the separation of chlorotoluene and bromotoluene position isomers. These results revealed the feasibility of covalent coupling strategy to fabricate MONs-based stationary phases in GC, highlighting the potential of MONs in separation science.
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NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 25
·10621-10627
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IF 3.288
Xue Xiong,Meiling Qi
This work presents a novel column fabrication approach for capillary gas chromatography (CGC) through the condensation reaction of a hydroxyl-terminated polymer with a cross-linker inside a capillary column. In this work, polypropylene glycol (PPG 4000) and tris[3-(trimethoxysilyl)propyl]isocyanurate (ICS) were employed to fabricate the PPG-gel capillary column for GC analyses. Inside the column, a 3D cross-linked organogel network forms on the capillary inner surface by condensation of PPG 4000 with ICS under the given mild conditions. As a result, the PPG-gel column exhibited moderate polarity and showed distinctly advantageous resolving performance and inertness for carboxylic acids over the PPG column by the conventional static coating method. Also, it achieved high-resolution separations of the isomer mixtures of alkanes, alkylbenzenes and halobenzenes. Moreover, the PPG-gel column exhibited dramatically improved solvent stability because of its cross-linked organogel network. The organogel column displayed excellent column repeatability and reproducibility with the RSD% values in the range of 0.01–0.04% for run-to-run, 0.71–0.84% for day-to-day and 2.4–3.1% for column-to-column. The proposed organogel approach is facile and applicable to other analogous materials and has a promising future for wide applications.
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JOURNAL OF CHROMATOGRAPHY A
·
2016
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IF 3.926
Yunzhong Zheng,Meiling Qi,Ruonong Fu
This work presents the first example of utilization of graphitic carbon nitride (g-C 3 N 4 ) as stationary phase for capillary gas chromatographic (GC) separations. The statically coated g-C 3 N 4 column showed the column efficiencies of 3760 plates/m and weak polarity. Its resolving capability and retention behaviours were investigated by using the Grob test mixture, and mixtures of diverse types of analytes, and structural and positional isomers. The results showed superior separation performance of the g-C 3 N 4 stationary phase for some critical analytes and preferential retention for aromatic analytes. Specifically, it exhibited high-resolution capability for aromatic and aliphatic isomers such as methylnaphthalenes and dimethylnaphthalenes , phenanthrene and anthracene and alkane isomers. In addition, g-C 3 N 4 column showed excellent thermal stability up to 280 °C and good repeatability with relative standard deviation (RSD) values less than 0.09% for intra-day, below 0.23% for inter-day and in the range of 1.9–8.4% for between-column, respectively. The advantageous separation performance shows the potential of g-C 3 N 4 and related materials as stationary phase in GC and other separation technologies.
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MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Yang Liu,Jian Li,Jiawei Liu,Changwei Tang,Quan Bai
Metal-organic frameworks (MOFs) are promising packing materials in liquid chromatography, but their micropore character extremely prevents the separation performance. Herein, we proposed an acidic etching strategy to fabricate a hierarchically porous University of Oslo-66 (HP-UiO-66) coated silica shell-core composite by using phosphoric acid as an etchant. The prepared composite, namely HP-UiO-66@silica-PA, was characterized by SEM, TEM, FT-IR, and N 2 -adsorption–desorption isotherms, demonstrating the generation of mesopores in HP-UiO-66 shell with pore width ranging in 4–5 nm. The chromatographic performance of HP-UiO-66@silica-PA-1 (etched by 1 mL phosphoric acid for 2 h) as HPLC stationary phase was evaluated in reversed-phase mode, and the separations of three groups of small solutes were studied and compared with the microporous UiO-66@silica (MP-UiO-66@silica) column. The results proved the obviously improved performances with narrower peak width, better resolution, and higher symmetry were achieved by the HP-UiO-66@silica-PA-1 column. The faster diffusion and mass transfer kinetics in the HP-UiO-66 shell signally reduce the plate height and improve the column efficiency. Specifically, the plate number of o -xylene was 18,442 plates m −1 , which was about 18.5 times higher than that on the MP-UiO-66@silica column (993 plates m −1 ). Furthermore, good repeatability and reproducibility with RSDs of both retention time and peak area less than 0.5 % confirmed the high stability and outstanding reliability of the HP-UiO-66@silica-PA-1 column. This study demonstrates that the phosphoric acid etching strategy is simple, mild, and controllable to fabricate hierarchically porous MOFs-based stationary phase for enhancing the HPLC separation performance.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Ting Wang,Yuan-Yuan Cui,Cheng-Xiong Yang
Background As a class of boomingly developed porous material, microporous organic networks (MONs) demonstrated great prospects in HPLC because of their unique structures and excellent stabilities. However, the agglomerated, irregular, and sub-micron sized MONs obtained via the traditional synthesis methods inevitably cause high column pressure and low column efficiency, rendering the main obstacles for their applications in HPLC. Monolithic column has the characteristics of continuous porous structure, fast mass transfer rate, excellent permeability, and low column pressure. Therefore, the integration of MONs with monolithic column should be a potential way to extend the widespread applications of MONs in HPLC. Results In this work, we reported the first example of fabricating MON incorporated monolithic columns by employing methacrylic acid as the monomer, ethylene dimethacrylate as the cross-linking agent, and polyethylene glycol-6000 as the porogen for the improved HPLC separation of small organic molecules. Effects of preparation conditions for MON incorporated monolithic column, sample loading mass, column temperature, and mobile phase composition were studied in detail. MON monolithic column after MON incorporation exhibits improved permeability, column efficiency, hydrophobicity, and specific surface area. Diverse small organic molecules such as polycyclic aromatic hydrocarbons, alkylbenzenes, acidic phenols, basic amines, phenylurea herbicides, parabens, phthalates, and flavonoids were successfully separated on MON incorporated monolithic column (50.0 mm length × 4.6 mm i.d.). MON incorporated monolithic column also provided better resolution and selectivity than C18 packed column. Significance This work reported the first combination of MON and monolithic column for efficient HPLC separation of variety small organic compounds with good resolution, selectivity, and precisions based on typical reversed phase mechanism. The experimental results demonstrated the great potential of MON incorporated monolithic column for enhanced HPLC separation of small organic compounds and could extend the new applications of MONs in separation science.
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Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
Wang Dan,Huang Guifang,Yu Chunming,Wang Yawen,Baek Nawon,Zhu Ruofei
The emergence of environmental endocrine disruptor chlorobenzene (CB) in surface water and its potential environmental impacts have attracted serious global attention. It is still very difficult to achieve effective degradation of it by catalytic oxidation process under mild conditions. Here, an optimized method for degrading CB in aqueous solution using Trametes versicolor laccase and surfactant-assisted laccase-mediator (SALM) system was investigated. The use of a Tween 80 surfactant enhanced the solubility of CB and promoted its efficient degradation. Under favorable conditions, the SALM system yielded a degradation efficiency of 43.5% and a dechlorination efficiency of 41.55% for CB (25 mg/L) within 24 h. The possible degradation pathway of CB by this system was speculated by detecting the intermediates produced during the reaction. The outcome of the proliferation assays on MCF-7 human breast cancer cells demonstrated a reduction in the estrogenic activity of the CB solution following treatment with the SALM system. Furthermore, the influence of the quantity and positional variation of chlorine substituents on the degradation process was methodically investigated. Moreover, molecular analyses were employed to study the detailed interaction mechanism between laccase and CB, which revealed that the hydrophobic interaction contributed dominantly to binding process. These findings provide an efficient and environmentally friendly degradation system for the development of purification strategies for halogenated pollutants.
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RSC Advances
·
2017
·Vol. 7
·期 16
·9662-9668
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IF 3.108
Yueyue Shen,Shilin Zhao,Yue Li,Qian Liu,Chongdi Ma,Hui Mao,Yang Liao,Jun Ma
Humic acid (HA), one of most ubiquitous natural resources in the world, was modified with monoolein to prepare a novel hydrophobic adsorbent (HA–M) for removal of chlorobenzene compounds (CBs) from aqueous solutions. The influence of pH, temperature, coexisting organics and initial concentration on the adsorption capacity of HA–M for CBs were investigated to obtain the optimum removal efficiency. Furthermore, the adsorption kinetics and isotherms were researched as well as the column adsorption studies to reveal the mechanism for HA–M to adsorb CBs. The experimental results indicated that the HA–M materials exhibit high removal efficiency of CBs (>90%) in a wide range of pH values and temperatures. The adsorption of CBs with HA–M was realized by the hydrophobic interactions between CBs and HA–M. In addition, the HA–M also performed high removal efficiency toward organochlorine pesticides (OCPs) in aqueous solutions. Most important, the excellent adsorbability of the super-hydrophobic HA–M adsorbent could be comparable or fairly good compared with other adsorbents. Consequently, our present investigations provided a facile synthesis route for the highly effective and recyclable hydrophobic adsorbent for the adsorptive removal of CBs and OCPs from aqueous solutions.
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RESEARCH ON CHEMICAL INTERMEDIATES
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2025
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IF 3.5
He Yan,Du Qingwang,Wen Shuna,Yu Tong,Wang Na,Yuan Dingzhong,Na Bing
In this work, an amine functionalized porous organic polymer (POP-ABP-NH 2 ) was synthesized by one-step Friedel–Crafts alkylation reaction using 2-aminobiphenyl as an organic monomer and p-dichlorobenzyl as a crosslinker. Then, POP-ABP-NH 2 was functionalized with different concentrations of phytic acid (PA, 30% and 50%) through hydrothermal reaction, and new phytic acid-modified porous organic polymers (POP-ABP-PA-1 and POP-ABP-PA-2) were successfully synthesized. The adsorption performance of the adsorbents for U(VI) ions in aqueous solution and simulated seawater was studied. The results show that the higher PA concentration, the smaller the specific surface area ( S BET POP-ABP-PA-2 (185.84 m 2 /g) < S BET POP-ABP-PA-1 (598.43 m 2 /g)). This may be because the high concentration of PA occupies more pore sites, resulting in a decrease in specific surface area. The adsorption kinetics of the materials for U(VI) conform to the pseudo-second-order kinetic adsorption model, and the adsorption isotherm conform to the Langmuir monolayer adsorption model. Under the optimal adsorption conditions, the maximum adsorption capacity of POP-ABP-PA-2 for U(VI) (q max = 1372.2 mg/g) is much higher than that of POP-ABP-PA-1 (q max = 769.2 mg/g). This may be because POP-ABP-PA-2 is connected to a high concentration of PA and has a high phosphorus and oxygen content, resulting in stronger coordination ability and higher adsorption capacity. Most importantly, in simulated seawater at pH = 8, POP-ABP-PA-2 exhibited a high theoretical adsorption capacity of 299.4 mg/g in 150 min. In addition, in a solution where many impurity ions coexist, POP-ABP-PA-2 can still exhibit excellent adsorption selectivity for U(VI), and after five adsorption–desorption cycles, the adsorption removal percentage of U(VI) can still be maintained at 90%. This study provides an important research idea for further developing phytic acid (PA) composites for U(VI) adsorption.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yange Li,Zhenlong Zhang,Huafang Zhang,Yuefeng Liu,Huiping Gao,Yanli Mao
Most of the reported perovskite solar cells (PSCs) with high efficiency are based on the (100) facet-dominated FAPbI3 perovskite films due to their easy formation. Recent reports demonstrate that the (111) facet is more stable and more invulnerable to cationic migration than the (100) one. However, the fabrication of (111) facet-dominated perovskite is challenging. Herein, we prepared the (111) facet-dominated perovskite films by a two-step method. In the first step, an additive of 6,6′-dimethyl-2,2′-bipyridyl (DMBP) was added to the PbI2 solution, which interacts with PbI2 by a chemical bonding between Pb+ and N– in its pyridine rings, regulating the microstructure of the PbI2 film. The experimental results demonstrate that the obtained perovskite film is (111) facet-dominated. Moreover, the DMBP additive reduces the number of defects and improves the energy band structure of the perovskite. The devices based on the (111) facet-dominated perovskite achieve a high efficiency of 24.11%, which is larger than that of the control (22.55%). In addition, the stability of the devices is enhanced by the DMBP additive.
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CHINESE CHEMICAL LETTERS
·
2025
·
IF 8.9
Ming-Mai Bao,Ying-Chao Liu,Hao-Nan Xu,Jing Zhang,Yin-Long Guo
Differentiating structural isomers in mass spectrometry (MS) poses significant challenges due to the difficulties in generating fragment ions by atmospheric pressure ionization and the spectral similarities encountered in tandem MS/MS analysis. The continuous arc plasma enables in-source dissociation fingerprinting analysis of isomers but presents a risk of solvent ignition during sample introduction because of high temperatures, posing safety concerns. To address these challenges, the Tunable Pulsed Arc Plasma (TPAP) ionization and dissociation device has been developed. The TPAP device converts continuous arc plasma into a pulsed mode, reduces plasma temperatures, enhances discharge stability and safety, and enables adjustable plasma energy for in-source compound dissociation. Programmable control over the pulsed arc plasma voltage allows the scanning of compounds under varying dissociation energies, facilitates the construction of compound-specific energy-resolved spectra. Validation experiments with disubstituted benzene species, chlorogenic acid derivatives, disaccharide and diacylglycerol have demonstrated the capability of TPAP-MS to effectively differentiate isomers through distinct energy-resolved spectra. The TPAP-MS requires minimal instrumental modifications and provides rapid and precise structural annotations directly from the ion source, offering broad potential applications in the analysis of structural isomers.
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JOURNAL OF CHROMATOGRAPHY A
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2025
·
IF 4
Jinhua Xu,Minghui Tang,Jinyu Zhang,Wenmin Zhang,Shiye Xie,Lan Zhang
This study reports the synthesis and application of a flower-like chiral metal-organic framework (CMOF) based on copper ions and tryptophan (Cu(Trp)-CMOF). The Cu(Trp)-CMOF was successfully synthesized at room temperature through a facile method with hexadecyl trimethyl ammonium bromide (CTAB) as a structure-directing agent. The material exhibits a flower-like morphology with hierarchical porosity and well-defined crystallinity in the monoclinic P2 1 /c space group. Comprehensive characterization revealed that different optical configurations ((R)-type, (S)-type, and (RS)-type) could be selectively obtained by controlling the chirality of tryptophan ligands. The synthesized CMOFs demonstrated excellent performance in two key applications: colorimetric recognition of amino acid enantiomers and separation of volatile organic compounds (VOCs). When used as a chromatographic stationary phase, the CMOF significantly enhanced the separation of challenging structural isomers, particularly improving the resolution (R S ) of xylene isomers (R S from 1.03 to 2.62) and ethyltoluene isomers (R S from 4.83 to 9.23) compared to conventional columns. This work provides new insights into the design and application of CMOFs for chiral separation and recognition.
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