JOURNAL OF LUMINESCENCE
·
2015
·
IF 2.719
Qi Liu,Aizhen Huang,Nan Wang,Guan Zheng,Lihua Zhu
Analysis of perfluorooctanoic acid (PFOA) usually requires a combination of high-performance liquid chromatography and mass spectrometry , which is expensive and time-consuming. In the present work, water-soluble CdS quantum dots (QDs) were employed to develop a simple and rapid fluorometric method for the determination of PFOA. Strongly fluorescent CdS QDs were prepared by using 3-mercaptopropionic acid (MPA) as a stabilizer . It was observed that PFOA strongly quenched the fluorescence emission of the MPA-CdS QDs because PFOA promotes the aggregation of MPA-CdS QDs through a fluorine–fluorine affinity interaction. Under optimum conditions, the fluorescence intensity of MPA-CdS QDs was observed to decrease linearly with an increase in the concentration of PFOA from 0.5 to 40 μmol L −1 , with a limit of detection of 0.3 μmol L −1 . This new method was successfully implemented for the analysis of PFOA-spiked textile samples, with recoveries ranging from 95% to 113%.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
POWDER TECHNOLOGY
·
2024
·
IF 4.5
Zhihao Shen,Qicheng Feng,Shuming Wen,Han Wang,Jing Cao,Zhengyong Song
The separation of polymetallic sulfide ores by flotation is a difficult task in the mineral processing industry. In this investigation, a small-molecule organic compound, mercaptosuccinic acid (MSA), was used as a new depressant for inhibiting galena in chalcopyrite flotation. Micro-flotation and surface wettability tests indicated that MSA pronouncedly weakened the floatability of galena but not chalcopyrite, resulting in marked differences in their flotation recovery. Findings of Fourier transform infra-red (FT-IR) spectroscopy measurements and reagent adsorption trials showed that MSA was strongly adsorbed on galena, resulting in the inability of the collector to effectively interact with galena. Atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS) analyses further corroborated that MSA showed a greater affinity for galena than chalcopyrite, this behavior markedly altered the superficial chemical environment of galena. Molecular dynamic simulations (MDS) showed that MSA increased the concentrations of H 2 O molecules on galena, which enhanced the surface hydrophilicity of galena.
研究领域:
矿物加工工程
·
表面化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Linlin Hu,Xing Wei,Jie Meng,Xiaoyan Wang,Xuwei Chen,Jianhua Wang
The development of fluorescent probes for detection of glutathione (GSH) has attracted extensive attentions due to its close association with cellular functions and diseases. Herein, a fluorescence enhancement sensing system based on acetaldehyde-modified-cystine (AMC) is reported for the detection of thiols, providing detection limits of 36 and 52 μmol L −1 for GSH and cysteine (Cys) respectively. Importantly, the fluorescence response of AMC is highly selective toward thiols with respect to the coexisting species in real biological matrixes. Considering the fact that GSH levels in cancer cells is significantly higher than those of Cys and other reducing species, the fluorescence enhancing response of AMC in cancer cells is dominated by GSH. Therefore, this provides the possibility for selective intracellular GSH imaging in living cancer cells, i.e., HeLa cells in the present study.
研究领域:
生物化学
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医学诊断
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细胞生物学
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产品关联
DOI
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 4
·506
·
IF 4.034
Shuquan Chang,Xian Wu,Jianzhang Lan,Zheng Li,Xiaohong Zhang,Haiqian Zhang
Quantum dots (QDs) have attracted great attention due to their unique optical properties. High fluorescence efficiency is very important for their practical application. In this study, we report a simple and efficient strategy to enhance the photoluminescence of water-dispersed thiol-capped QDs using γ-radiation. Three kinds of QDs with different surface ligands and cores (MPA-CdTe, MPA-CdSe and Cys-CdTe) were fabricated and irradiated by high-energy γ-ray in an aqueous solution. Their photoluminescence intensities were significantly enhanced after irradiation, which were closely related to the radiation dose and the structure of QDs. The positions of the fluorescence emission peaks did not shift obviously after irradiation. The mechanism of photoluminescence enhancement was discussed based on the results of photoluminescence (PL) spectra, UV-visible light absorption (UV-vis) spectra, transmission electron microscope (TEM), X-ray diffraction (XRD) patterns, Fourier transform infrared (FT-IR) spectra and X-ray photoelectron spectroscopy (XPS). This method can be employed to uniformly treat large batches of QDs at room temperature and without other chemicals.Keywords:quantum dots;luminescence enhancement;γ-radiation;thiol-capped
研究领域:
材料科学
·
光学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 73
·59056-59061
·
IF 3.84
Lun Song,Haiyu Tian,Xiaoliang Pei,Ziyou Zhang,Weibing Zhang,Junhong Qian
Two water-soluble colorimetric and turn-on fluorescent probes STP1–2 were rationally designed and synthesized for selective recognition of GSH. Both probes are colorless 4-thioether-1,8-naphthalimide derivatives, which are almost non-fluorescent due to the photoinduced electron transfer from the fluorophore to the 4-nitrobenzene. Upon addition of GSH to STP1/STP2–CTAB solution, obvious spectral responses were observed: the absorption peak shifted from 345 nm to 390 nm companied with ∼90-fold fluorescence enhancement at 487 nm. A good linear relationship between the fluorescence intensity and GSH concentration was obtained, and the detection limit of GSH was estimated to be 8.40 × 10−8 mol L−1. The experimental results imply that both probes could be applied in fluorescent imaging of GSH within living cells and in the detection of mercapto-containing proteins as well.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Tingting Hong,Zhiwen Tan,Tian Yang,Dan Xu,Yamei Jin,Fengfeng Wu,Xueming Xu
The impact of adding zein on the gluten network during extruded noodle processing and the quality of the noodles by extrusion-cooking were investigated. These changes were studied by size exclusion-high performance liquid chromatography (SE-HPLC), free sulfhydryl (SH) measurement, non-covalent interactions, Fourier-transform infrared spectroscopy (FTIR), particle size analysis, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM). Zein reduced the protein aggregation, resulting in a higher free sulfhydryl content and increased hydrogen bonds and hydrophobic interactions during noodle processing. Moreover, Zein reduced the relative content of β-sheets by 4.54% and 7.48% at the extrusion and cooking stages, respectively. Zein decreased the particle size during extrusion but increased it during cooking. Zein improved the compactness and strength of noodle microstructure during cooking, exhibited higher hardness and impeded water mobility compared to extruded noodles made only with wheat flour (WF). The extruded noodles containing zein showed a significant increase of 26.10% and 61.11% in tensile strength and distance, respectively. Zein did not cause significant changes in the cooking loss and water absorption in the extruded noodles. Research on the impact of zein on protein during the processing of extruded noodles could provide a dependable approach and valuable mechanistic insights for improving the quality of extruded noodles.
研究领域:
食品科学
·
分子生物学
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Xiang-Nan He,Ya-Ning Wang,Yue Wang,Zhang-Run Xu
Sialic acid (SA) is a special monosaccharide widely distributed at the termini of sugar chains on the cell surface, and its expression level is closely connected with various biological and pathological processes. Therefore, accurate quantitative detection of SA on cancer cell surface is of great significance for clinical diagnosis and therapy. Here, we developed a whole-surface accessible method of accurate SERS quantification of SA level on a single cell, in which silver nanoparticles functionalized with 4-mercaptophenylboric acid and 4-mercaptobenzenitrile was used as the background-free SERS probe. The cyano group on the nanoprobe showed a unique Raman shift at 2232 cm −1 , where most of the biological samples have no Raman response. Meanwhile, the boronic acid group had high specificity to SA molecules at physiological pH. The expression level of SA can be accurately quantitated on the basis of the C N Raman signal. The average number of expressed SA molecules on the surface of a single HeLa cell was 4.6 × 10 7 . And SERS imaging of a single cell was achieved at 2232 cm −1 without biological interference. We evaluated SA expression level on the surface of different cancer cells and dynamically monitored SA expression under the influence of drugs. The proposed approach is accurate as well as sensitive for background-free quantification of SA on cell surface, which is promising for revealing the relationship between tumors and cell surface glycosylation .
研究领域:
分析化学
·
生物医学工程
·
癌症研究
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
AICHE JOURNAL
·
2023
·Vol. 69
·期 5
·e18023
·
IF 3.7
Qing Sun,Yan Dong,Shuai Wang,Zhanfang Cao,Xin Ma,Hong Zhong
Modified surfactants can regulate their adsorption at the target surface and are essential for various separation applications such as froth flotation. Thioether-containing hydroxamic acid (BTHA) is an excellent oxidized-ore collector with poor dispersibility. Herein, acylamino, which possesses the ability to improve the surfactants' self-assembly and dispersion, was introduced to synthesize N-[6-(hydroxyamino)-6-oxohexyl] benzylthioacetamide (BTAHA); thus, applying it to cassiterite flotation. Surface tension, adsorption capacity, in situ AFM, and surface energy results reveal BTAHA displays strong bubble stability and readily adsorbs onto the mineral's surface, promoting hydrophobic flotation. BTAHA is an effective cassiterite collector and has good separation effects on cassiterite against calcite. It can form Van der Waals forces and five-membered hydroxamate-(O,O)-Sn rings with Sn atoms on cassiterite surfaces via thioether and hydroxamate groups. Meanwhile, the presence of intermolecular hydrogen bond between the amide groups facilitates the self-assembly of the collectors on the cassiterite surface, and has broad industrial application prospects in various separation processes.
研究领域:
矿物加工工程
·
界面化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CRYSTAL GROWTH
·
2025
·
IF 2
Van-Thao Ta,Duy-Khanh Nguyen,Xuan-Bach Nguyen,Phuong-Nam Nguyen,Sinh-Hung Nguyen,Xuan-Dung Mai
The development of a simple, cost-effective, and green method to synthesize CdTe quantum dots (QDs), which are a key nanomaterial in sensing, bioimaging, and immunofluorescence analysis is highly desirable. In this study, we used ammonia in lieu of common hydride reductant to synthesize CdTe QDs by simple refluxing aqueous solutions of cadmium salt, sodium tellurite, and 3-mercaptopropionic acid (MPA). The obtained CdTe QDs were characterized by absorption and photoluminescent spectroscopies, X-ray diffraction (XRD), and transmission electron microscope (TEM). The absorption window and the emission color of QDs were facile controlled over the green–red range by varying the refluxing time. As the QD size increased the crystal structure transformed from CdTe to a CdTeS alloyed form. The results demonstrated herein suggest a cost-effective method for production of CdTe QDs.
研究领域:
材料科学
·
纳米技术
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产品关联
DOI
高影响因子
Organic Chemistry Frontiers
·
2024
·
IF 4.6
Yueyue Xing,Tianyu Bo,Nan Zhang,Meiqi Wu,Jiawei Wang,Shigang Shen,Yafang Wang,Changying Song,Tiesheng Shi,Shuying Huo
Peptides constrained by multiple disulfide bonds (MDBs) or by thioether bridges play a critical role in improving the biological and pharmaceutical activities of peptide drugs. The synthesis of correct MDBs and thioether-bridged bicyclic peptides still remains a significant challenge. In this work, benzeneseleninic acid (BSA) acting as both an oxidant and a deprotecting reagent for the synthesis of MDB- and thioether-bridged bicyclic peptides has been investigated. Disulfide bonds in peptides can be formed by direct oxidation of two sulfhydryl groups by BSA in neutral media or via two concerted steps, namely deprotecting two acetamidomethyl (Acm) groups and oxidation by BSA in acidic media. As such, two disulfide bonds in α-conotoxin SI, apamin, α-conotoxin IMI and a peptide containing a methionine residue were synthesized regioselectively by the use of the BSA oxidation and deprotection reaction (BSA-ODr). By utilization of the BSA deprotection property, bicyclic peptides were synthesized based on the crosslinking of xylylene dibromide with sulfhydryl groups. Furthermore, two disulfide bonds in α-conotoxin SI and three disulfide bonds in conotoxin mr3e, enterotoxin STp, μ-conotoxin KIIIA, linaclotide and ziconotide were also synthesized regioselectively through oxidation of fully reduced peptides by BSA. All the reactions were carried out under mild conditions in a one-pot manner and peptides with satisfactory yields were achieved. In addition, the BSA-ODr is compatible with methionine residues in the peptides. Moreover, the relative positions of two Acm-protected cysteines and two free cysteines have no impact on the BSA-ODr approach for the construction of two disulfide bonds in peptides. The oxidative folding strategies based on BSA can be executed in peptide manufacture. BSA is readily accessible conferring efficient BSA-ODr and oxidative folding methodologies for the synthesis of MBDs and thioether bridges in peptides.
研究领域:
生物化学
·
药物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yuanchao Lu,Xiaohua Nie,Zeyi Wu,Chengyu Jin,Ningxiang Yu,Xianghe Meng,Qin Ye
Food matrices greatly impact TBBQ content during digestion, while lacking sufficient research and understanding. This study investigated the influence and mechanism of fried foods on the TBBQ-eliminated performance during in-vitro digestion. The results indicated that TBBQ content varied significantly among food matrices after in-vitro digestion, with the highest in peanuts (38.3%). The correlation analysis revealed that proteins remarkably facilitated TBBQ-eliminations while fats decreased the TBBQ-eliminated rate. The TBBQ-eliminated performance of proteins, protein digestive mixtures, and amino acids uncovered that sulfhydryl groups were crucial reactive groups to eliminate TBBQ, and TBBQ-eliminated rates under intestinal pH (8.0) were faster than gastric pH (1.5). Additionally, fats significantly reduced the protein-triggered TBBQ-eliminations, originating that the oil–water interface increased the interaction difficulty between lipophilic TBBQ and proteins. Thus, this work provided an in-depth understanding of food matrices (especially proteins and fats) in TBBQ eliminations to enlighten the promising TBBQ-risk-reduced strategies with high-protein and low-fat foods.
研究领域:
食品科学
·
营养学
·
化学
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 17
·2986-2994
·
IF 5.342
Wen Jing Yang,Wenya Xu,Xi Tao,Wen Wang,Yaqin Hu,Xue Li,En-Tang Kang,Lianhui Wang
The fabrication of adhesive hydrogel systems is quite important for biological applications. Herein, two-stage thiol-based click reactions have been employed for the preparation and adhesion of hydrogels. In the first stage, two self-adhesive hydrogels (HG-1 and HG-2) were constructed via the thiol-based click reactions and thiol–thiol coupling reaction under different stimuli, employing the same functional copolymer poly(propargyl methacrylate-co-N-(3,4-dihydroxyphenethyl) methacrylamide-co-poly(ethylene glycol) methyl ether methacrylate) (poly(PMA-co-DMA-co-PEGMA), polyPDP) and a 4-arm thiol-PEG cross-linker. Subsequently, the two prepared hydrogels achieved responsive self-adhesion properties via the second stage of thiol-based click reactions between the preserved functional groups under different stimuli. Moreover, the self-adhered hydrogels exhibited increased mechanical strength after adhesion. The present strategy thus provides a facile approach for the fabrication of hydrogel systems with on-demand self-adhesive properties.
研究领域:
生物材料
·
化学工程
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）