SOUTH AFRICAN JOURNAL OF BOTANY
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2024
·
IF 2.7
Kainat Bilal,Fatima Mehboob,Nosheen Akhtar,Irfan Ali Mirza,Muhammad K. Okla,M. Junaid Dar,Ibrahim A. Saleh,Naser Zomot,Humaira Fatima
The current study aimed to evaluate the in vivo wound healing potential of Psidium guajava L. by wound excision model owing to its ethnomedicinal use as a wound healer since olden times. P. guajava leaves were extracted using methanol-chloroform (1:1). Phytochemical analysis of P. guajava leaves extract (PGLE) was performed using total phenolic content (TPC) assay, total flavonoid content (TFC) assays, and High-performance liquid chromatography (HPLC). In vitro , antioxidant potential (DPPH radical scavenging assay, total antioxidant capacity (TAC) assay, and ferric reducing antioxidant power (FRAP) assay), antibacterial activity (broth microdilution method), and hemolytic analysis of PGLE were performed. In vivo , wound healing proficiency of PGLE (10, 5, and 2.5% w/w) was determined by estimating hydroxyproline and endogenous antioxidant markers. TPC and TFC were found to be 57.5 ± 0.37 µg gallic acid equivalent/mg extract and 32.1 ± 0.24 µg quercetin equivalent/mg extract, respectively. A prominent concentration of polyphenols was demonstrated by HPLC-based quantification with the highest value of rutin which is 12.3 ± 0.12 µg/mg extract. A noteworthy DPPH radical scavenging activity with an IC 50 of 40.7 µg/ml, TAC, and FRAP i.e., 159.3 ± 0.11 and 206.1 ± 0.17 µg ascorbic acid equivalent/mg extract, respectively were exhibited by PGLE. Significant antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeroginosa, and Methicillin-resistant Staphylococcus aureus with the minimum inhibitory concentration of 250, 400, 400, and 500 µg/ml respectively was depicted by PGLE and found biocompatible with 1.1 ± 0.16% hemolysis. Significant wound contraction, faster epithelialization (9 ± 1 days), extensive fibroblast proliferation, and substantial neovascularization were observed in the 10% PGLE-treated group also evident by histopathological examination. Significant hydroxyproline (15.7 ± 1.02 µg/mg tissue), catalase, peroxidase (5.4 ± 0.26 and 6.1 ± 0.35 U/mg tissue) and glutathione content (9.7 ± 0.06 mmol/mg tissue) with p-value of less than 0.01 was demonstrated by 10% PGLE. A reduction in lipid peroxidation (0.5 ± 0.07 mmol/mg tissue) was also observed at the same concentration (10%). These findings scientifically validate the potent pharmacological effects of P. guajava phytochemicals and suggest their promising role as wound healing agents.
研究领域:
药理学
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植物化学
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伤口修复
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DOI
高影响因子
Marine Drugs
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2012
·Vol. 10
·期 12
·2634-2647
·
IF 3.854
Jiaojiao Zheng,Yicun Chen,Fen Yao,Weizhou Chen,Ganggang Shi
Gloiopeltis tenax(G. tenax) is widely distributed along the Chinese coastal areas and is commonly used in the treatment of diarrhea and colitis. This study aimed at investigating the bioactivities of the volatile constituents inG. tenax. We extracted the essential constituents ofG. tenaxby supercritical carbon dioxide extraction (CO2-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 30 components were identified in theG. tenaxextract. The components showed remarkable antioxidant activity (radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH)), lipid peroxidation inhibition capacity (in a β-carotene/linoleic acid-coupled oxidation reaction), and hydroxyl radical-scavenging activity (by deoxyribose degradation by iron-dependent hydroxyl radical), compared to butylated hydroxytoluene. In microdilution assays,G. tenaxextracts showed a moderate inhibitory effects onStaphyloccocus aureus(minimum inhibitory concentration (MIC) = 3.9 mg/mL),Enterococcus faecalis(7.8 mg/mL),Pseudomonas aeruginosa(15.6 mg/mL), andEscherichia coli(3.9 mg/mL). Antioxidant and antimicrobial activities ofG. tenaxwere related to the active chemical composition. These results suggest that the CO2-SFE extract fromG. tenaxhas potential to be used as a natural antioxidant and antimicrobial agent in food processing.Keywords:Gloiopeltis tenax;GC-MS;CO2-SFE;antioxidant activity;antimicrobial activity
研究领域:
海洋生物学
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食品科学
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药用植物学
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2019
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IF 3.261
Hong Yan,Zengwei Zhu,Yumei Long,Weifeng Li
Graphitic carbon nitride (g-C 3 N 4 ) with desired microstructure and chemical functionalities has been drawing considerable interests in terms of their potential applications for environmental treatment and clean energy production. Herein, a novel structure of MoO 3-x ―intercalated g-C 3 N 4 (Mo-g-C 3 N 4 ) and the synthesis strategy were demonstrated by using melamine molybdate as a precursor. The single-source precursor of melamine molybdate ensured the homogeneous mixing between MoO 3-x and g-C 3 N 4 at molecular level. Meanwhile, the strongly chemical interaction between melamine and molybdate promoted the in-situ intercalation of MoO 3-x into g-C 3 N 4 interlayer. The intercalation of MoO 3-x not only improved the visible-light absorption ability, but also facilitated charge separation through interlayer pathway for g-C 3 N 4 . As expected, the Mo-g-C 3 N 4 possesses enhanced activity in the photo-degradation of methyl orange and photo-destruction of Escherichia coli ( E. coli ) bacteria illuminated by visible light. The radicals trap experiments revealed that the holes (h + ) are dominant species in the photocatalysis process over Mo-g-C 3 N 4 . Accordingly, a possible photocatalysis mechanism of pollutants over Mo-g-C 3 N 4 was developed. This study opens up a new way for optimizing microstructure of g-C 3 N 4 -based materials with favorable photocatalytic properties.
研究领域:
材料科学
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环境科学
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化学
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产品关联
DOI
高影响因子
MOLECULES
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2018
·Vol. 23
·期 7
·1821
·
IF 3.098
Yejing Wang,Rui Cai,Gang Tao,Peng Wang,Hua Zuo,Ping Zhao,Ahmad Umar,Huawei He
Silk sericin is a protein from a silkworm’s cocoon. It has good biocompatibility, hydrophilicity, bioactivity, and biodegradability. However, sericin could not be used in biomedical materials directly because of its frangible characteristic. To develop multifunctional sericin-based materials for biomedical purposes, we prepared a sericin/agar (SS/agar) composite film through the blending of sericin and agar and repetitive freeze-thawing. Then, we synthesized silver nanoparticles (AgNPs) in situ on the surface of the composite film to endow it with antibacterial activity. Water contact angle, swelling and losing ratio, and mechanical properties analysis indicated that the composite film had excellent mechanical property, hydrophilicity, hygroscopicity, and stability. Scanning electron microscopy and X-ray photoelectron spectroscopy analysis confirmed the successful modification of AgNPs on the composite film. X-ray powder diffraction showed the face-centered cubic structures of the AgNPs. This AgNPs modified composite film exhibited an excellent antibacterial capability againstEscherichia coliandStaphylococcus aureus. Our study develops a novel AgNPs/sericin/agar composite film with enhanced mechanical performance and an antimicrobial property for potential biomedical applications.Keywords:silk sericin;agar;silver nanoparticles;antimicrobial activity
研究领域:
生物材料学
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医学材料
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Najaf Ali Soomro,Qiao Wu,Safdar Ali Amur,Hao Liang,Aziz Ur Rahman,Qeping Yuan,Yun Wei
In this study, we have encapsulated natural physcion (PHY) drug loading into metal-organic frameworks MOFs , zeolitic imidazolate frameworks (ZIFs) through straight-forward nano-precipitation technique. The synthesized PHY@ZIF-8 indicated high drug loading encapsulation efficiency i.e. 88%, whereas, drug loading capacity was found to be 11.49%. The characterization of PHY loaded-ZIF 8 (PHY@ZIF-8) was carried out by powder x-ray diffraction (PXRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermal gravimetric analysis (TGA), and FT-IR methods. The release of PHY loaded in ZIF-8 was 88.72% at pH 5.0 which is approximately three time higher than its release in physiological system with pH 7.4 (27.61%). The remarkable stability of PHY@ZIF-8 NPs even after 25 days stem it as an effective and stable candidate. Furthermore, the antibacterial activity of pure PHY, ZIF-8 and PHY@ZIF-8 were investigated against gram negative strains and gram positive strain. The PHY@ZIF-8 showed maximum growth inhibition zones against all microorganism as compare to pure PHY. We hope that this model drug could have the potential ability for treatment of various infectious diseases.
研究领域:
材料科学
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药理学
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微生物学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2015
·
IF 1.098
Zujin Yang,Yanxiong Fang,Hongbing Ji
Microcapsules of salicylic acid (SA) with chitosan were prepared by spray drying method. Various analytical methods were used to characterize the nature of microcapsules. Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of intermolecular interactions between chitosan and SA. Particle size analysis showed that the average size of microcapsules ranged from 2 to 20 μm. Scanning electron microscopy (SEM) studies indicated that the microspheres were spherical and had a relatively smooth surface. Microbiological assay of antibacterial activity for SA and its microcapsules was measured using different bacterial strains. It was found that the antibacterial activity of SA was improved after the formation of microcapsules. The in vitro release profile showed that the microcapsules could control SA release from 1 h to 4 h. Kinetic studies revealed that the release pattern follows Korsmeyer–Peppas mechanism. Enhanced antibacterial activity of the SA microcapsules was attributed to the synergistic effects of intermolecular hydrogen-bonding interactions N–H⋯O and O–H⋯O C between SA and chitosan. It was also confirmed by quantum chemical calculation.
研究领域:
药剂学
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微生物学
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产品关联
DOI
高影响因子
JOURNAL OF FISH DISEASES
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2022
·Vol. 45
·期 11
·1599-1607
·
IF 2.58
Xin-Yuan Jiang,Zong-Yi Ren,Nian-Kun Zhang,Ke-Chen Yang,Gao-Xue Wang,Hai-Feng Jiang
Nocardiosis caused by Nocardia seriolae is a major threat to the aquaculture industry. Given that prolonged therapy administration can lead to a growth of antibiotic resistant strains, new antibacterial agents and alternative strategies are urgently needed. In this study, 80 medicinal plants were selected for antibacterial screening to obtain potent bioactive compounds against N. seriolae infection. The methanolic extracts of Magnolia officinalis exhibited the strongest antibacterial activity against N. seriolae with the minimal inhibitory concentration (MIC) of 12.5 μg/ml. Honokiol and magnolol as the main bioactive components of M. officinalis showed higher activity with the MIC value of 3.12 and 6.25 μg/ml, respectively. Sequentially, the evaluation of antibacterial activity of honokiol in vivo showed that honokiol had good biosafety, and could significantly reduce the bacterial load of nocardia-infected largemouth bass ( p < .001). Furthermore, the survival rate of nocardia-infected fish fed with 100 mg/kg honokiol was obviously improved ( p < .05). Collectively, these results suggest that medicinal plants represent a promising reservoir for discovering active components against Nocardia , and honokiol has great potential to be developed as therapeutic agents to control nocardiosis in aquaculture.
研究领域:
水产养殖学
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药用植物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Ke Hu,Rongyu Li,Feixu Mo,Yi Ding,Aiai Zhou,Xue Guo,Ruotong Li,Min Li,Minggui Ou,Ming Li
Dendrobium officinale Kimura et Migo is a traditional Chinese herbal medicinal plant . However, the frequent occurrence of soft rot disease (SRD) is one of the most harmful diseases in D. officinale production in recent years, which can seriously affect its yield and quality. In this study, the major pathogenic fungus (SR-1) was isolated from D. officinale with typical symptoms of SRD, and was identified as Fusarium oxysporum through morphological and molecular identification. The biological activities of five natural products were determined against F. oxysporum using a mycelial growth inhibition assay. The results showed that osthole had the highest antifungal activity against F. oxysporum , with an EC 50 value of 6.40 mg/L. Scanning electron microscopy (SEM) showed that osthole caused F. oxysporum mycelia to shrink and deform. Transmission electron microscopy (TEM) showed that the organelles were blurred and the cell wall was thickened in the presence of osthole. The sensitivity of F. oxysporum to calcofluor white (CFW) staining was significantly enhanced by osthole. Relative conductivity measurements and propidium iodide (PI) observation revealed that osthole had no significant effect on the cell membrane. Further experiments showed that the activity of chitinase and β-1,3-glucanase were decreased, and expression levels of chitinase and β-1,3-glucanase related genes were significantly down-regulated after treatment with osthole. In conclusion, osthole disrupted the cell wall integrity and dynamic balance of F. oxysporum , thereby inhibiting normal mycelial growth.
研究领域:
植物病理学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Min Liu,Xinyu Gao,Fei Pan,Yuwei Deng,Dongsheng Xia,Zhang Li,Jie Fu
This work reported the role of pyrene in formation of naturally occurring silver nanoparticles (AgNPs) via the reduction of silver ions by humic acid under the UV irradiation in the aquatic environment. An increase in temperature (25–90 °C), pH (5–9) or concentration of humic acid (2.5–15 mg/L) led to an enhanced formation of AgNPs. The TEM images indicated the formed AgNPs were spherical with an average particle size of ∼20 nm. Pyrene showed a limited capacity for the photoreduction of silver ions, and when both pyrene and humic acid were present, pyrene would compete with humic acid for the reduction of silver ions. However, the presence of pyrene would enhance the stability and suppress the antibacterial activity of natural AgNPs. The UV–vis spectra of AgNPs suspensions generated with pyrene did not change within 45 days. The inhibition rates against Escherichia coli of AgNPs generated with pyrene were 8–32% lower than those of AgNPs generated without pyrene. This study provides environmental implications on the fate and ecotoxicity of natural AgNPs with interaction of polycyclic aromatic hydrocarbons .
研究领域:
环境科学
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纳米生物学
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Jia Ke,Yingnan Zhu,Jiamin Zhang,Jing Yang,Hongshuang Guo,Weiqiang Zhao,Chiyu Wen,Lei Zhang
Silk is an attractive natural fiber due to its excellent mechanical properties and biocompatibility. Xenobiotic modification of pristine silk is gaining significant attention to meet specific demands. The silk payload of xenobiotics is affected by their uptake efficiency, biodistribution, metabolism, and excretion. Herein, we systematically evaluated the effect of the nanoparticle size (20–100 nm) on the biodistribution and silk production by introducing silver nanoparticles (AgNPs) into the silkworm diet. Interestingly, the silk payload of AgNPs is highly dependent on their overall size, where 50 nm AgNPs are prone to accumulate in silk glands and reveal the highest content in the produced silk, qualifying silk antibacterial activity against both Gram-positive and Gram-negative bacteria. These findings provide new insights to understand how nanoparticle properties influence the incorporation of nanomaterials into silk and can help optimize the material design.
研究领域:
生物材料学
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纳米生物学
·
昆虫学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Rui Cai,Mingquan Zhang,Lu Cui,Yahong Yuan,Yifei Yang,Zhouli Wang,Tianli Yue
Alicyclobacillus acidoterrestris has received much attention due to its unique thermo-acidophilic property and implication in the spoilage of pasteurized juices. The aim of this study was to investigate the antibacterial characteristics and mechanisms of thymol against A. acidoterrestris vegetative cells and spores. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of thymol against vegetative cells were both 0.25 mg/mL. MIC of thymol against spores was 0.5 mg/mL, and the MBC was greater than 1.0 mg/mL. Thymol could effectively inhibit A. acidoterrestris growth, spore germination and guaiacol production. When treated by thymol, the leakage of nucleic acids and proteins from A. acidoterrestris cells and spores increased significantly. SDS-PAGE investigation of bacterial proteins showed that the loss of soluble proteins was obvious as well. Scanning electron microscopy observation exhibited that A. acidoterrestris cells or spores treated with thymol had a severe morphological damage. These results demonstrated that thymol exerted its good antibacterial activities by destroying the integrity of the cell and spore. This study provides a new method for the inactivation of A. acidoterrestris in fruit juice.
研究领域:
食品科学
·
微生物学
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DOI
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Zhiyan Ma,Xinhua Zhu,Yingru Huang,Yufeng He,Pengfei Song,Rongmin Wang
In order to prevent the spread of the epidemics caused by pathogenic microorganisms, it is very important to explore antimicrobial materials. In this paper, a novel antimicrobial polymer materials with dual active centers, iodine-povidone- co -thiazole nanoporous membrane (IPoTaNm), was successfully synthesized by one-pot emulsion polymerization followed by iodine complexation. The micromorphology and composition were characterized by scanning electron microscope (SEM), Fourier transform infrared spectra (FT-IR), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS), X-ray powder diffraction (XRD) and contact angle test. Then, its antimicrobial performance was measured by using Escherichia coli and Staphylococcus aureus as test strains. It indicated that IPoTaNm exhibits excellent antibacterial activity, which the inhibition rate got to 99.9 % after 48 h. It also presented sustaining bactericidal activity, which the antimicrobial activity maintained 99.9 % even after >7 days. The synergistic antimicrobial mechanism of thiazole units (AcT) and iodine in IPoTaNm was discussed, and some facters for their inhibitory effects in the water column were investigated. In summary, the obtained IPoTaNm with dual active centers was an excellent candidate for minimizing bacteria-infection in different functional materials, which has potential applications in wastewater treatment to reduce bacterial infections.
研究领域:
材料科学
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生物学
·
环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）