MICROCHEMICAL JOURNAL
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2023
·
IF 4.8
Xueying Chen,Yuxiao Hu,Zhijian Tan
Traditional three-phase partitioning (TPP) systems used for extracting and purifying bioactive substances typically consist of t -butanol and ammonium sulfate. However, the use of volatile and flammable organic solvents and high-concentration inorganic salts results in environmental pollution. Therefore, an innovative TPP based on deep eutectic solvents (DESs) and sugars (sugaring-out effect) was used to extract and preliminarily purify cucumber peroxidase (POD). Various factors affecting the extraction were optimized using response surface methodology (RSM). DES-8 (menthol: octanoic acid = 1:1) and sucrose were selected as the optimal constituents of TPP. Under these conditions, the sucrose concentration was 36% (w/v), DES: crude extract (v/v) was 1.4:1, system pH was not adjusted, extraction time was 6 min, and extraction temperature was 31 °C; the enzyme recovery and purification fold reached 84.53% and 5.28, respectively. Recycling experiments demonstrated that the DESs can be well recycled and reused after five recycles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze cucumber POD, which showed that the TPP system achieved primary purification of cucumber POD. This TPP is much greener and more efficient than traditional TPP systems and shows great application potential in the enzymes and proteins separation field.
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Foods
·
2023
·Vol. 12
·期 7
·1369
·
IF 5.2
Fenghong Deng,Xiuting Hu,Yueru Wang,Shunjing Luo,Chengmei Liu
Rice bran, rich in feruloyl arabinoxylan, is a good source of feruloyl oligosaccharides (FOs). To prepare FOs, bran was often hydrolyzed by amylase and protease to remove starch and protein and then hydrolyzed by xylanase, which was time-consuming and had a low yield. To solve the above problems, enzymatic extrusion was used to treat rice bran, and the effects of traditional hydrolysis, a combination of traditional extrusion and hydrolysis (extrusion-hydrolysis) and enzymatic extrusion on the yield of FOs were investigated and compared in this study. It was found that traditional extrusion and enzymatic extrusion significantly increased the yield of FOs. Particularly, the yield of FOs resulting from enzymatic extrusion was increased to 5.78%, while the yield from traditional hydrolysis was 4.23%. Microscopy analysis showed that extrusion damaged the cell wall of bran, which might increase the accessibility of xylanase to arabinoxylan and the yield of FOs. Spectroscopy analysis suggested that FOs obtained by different pretreatments had similar structures. It was obvious that enzymatic extrusion saved the time for removal of starch and protein and increased the yield of FOs. In addition, the highest yield of FOs was found at the moisture content of 30% and the screw speed of 50 rpm. This study provided an efficient method for the preparation of FOs that is suitable for industrial production.Keywords:rice bran;feruloyl oligosaccharides;enzymatic extrusion;yield
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ChemistrySelect
·
2023
·Vol. 8
·期 16
·e202300902
·
IF 2.1
Panli Liu,Shengbin Shi,Ruiping Wei,Lijing Gao,Jin Zhang,Guomin Xiao
Graphical P-Zr-SBA-15 catalyst with bifunctional acidity was synthesized by introducing Zr 4+ into the mesoporous material SBA-15 structure for intra skeleton modification, and then impregnated with phosphoric acid solution to modify the outer skeleton. The catalyst exhibits excellent catalytic activity, good thermal stability, and recoverability in the preparation of furfural. A series of bifunctional P-Zr-SBA-15 catalysts with Lewis acid and Brønsted acid sites were prepared by modifying mesoporous material SBA-15 with Zr 4+ and phosphate group, which were used in the conversion of xylose and xylan into furfural. The structure and acidic characteristics of the catalyst were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, N 2 adsorption desorption, scanning/transmission electron microscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, NH 3 temperature programmed desorption, and pyridine adsorption infrared spectroscopy. The effects of different process parameters on the catalytic performance of the catalyst were studied. when using the xylose and xylan as substrates, the furfural yield were 80.3 % and 68.8 % under the optimal conditions in the H 2 O (NaCl) /toluene (3/3 mL) biphasic solvent system, respectively, and 52.5 % furfural yield was obtained using xylose as substrate in aqueous system. In addition, the catalyst has good thermal stability and easy recovery in the recycling experiment.
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JOURNAL OF FOOD ENGINEERING
·
2023
·
IF 5.5
Beibei Ye,Cikun Liu,Huan Li,Feifei Wang,Jian Chen,Yanbo Wang
The spoilage of seafood is highly dependent on temperature changes during cold chain. To visually reflect the shelf-life of large yellow croakers (0–25 °C), the xylose-lysine based time-temperature indicator (TTI) label was designed and applicated. The ultraviolet (UV) spectrum, and color response of Maillard reaction (MR) indicated that the color changes of TTI was related to the formation of melanoidins of MR. The color changes of TTI, and the quality degradation of large yellow croakers were described by kinetics analysis, which showed that Ea of TVB-N (70.45 kJ/mol) was closer to that of TTI (83.51 kJ/mol). Under fluctuating temperatures, the difference of the effective temperature (T eff ) between TVB-N (15.95 °C) and TTI (16.84 °C) was less than 1 °C, and the relative error of shelf-life was −8.33%. Briefly, this novel TTI label as an environmentally friendly system could be attached to sample surface for real-time reflecting shelf-life of seafood in cold chain.
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CATALYSIS COMMUNICATIONS
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2023
·
IF 3.7
Dan Luo,Qin Wang,Jiansu Ran,Ruixue Yangcheng,Yuntong Cui,Shuang Luo,Jianjian Wang
A series of Zr-based catalysts were prepared using various commercial and cheap organic acids as the coordination agents, and these as-synthesized samples exhibited superior activities to produce lactic acid (LaA) from carbohydrates. Using humic acid (HA) as a typical coordination agent, LaA yield was up to 76% over the resultant Zr-HA, outperforming the most recent reported works. Deeper studies revealed that Zr-HA possessed both acid sites and base sites, and these dual active sites can simultaneously activate xylose, resulting in high yield production of LaA. Besides, Zr-HA also showed excellent reusability and can be reused at least five times.
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INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Jingwen Sun,Yu Xie,Zhiyuan Chen,Yipin Fan,Yunfeng Liu,Qi Gao,Jianqiang Li,Jingwen Bai,Yu Yang
Magnolia officinalis root extract was obtained by high-pressure-assisted extraction, and the yield of honokiol (HNK) was 86.97 ± 1.05 mg/g dry power. The purified extracts and HNK inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Assays for alkaline phosphatases , proteins, nucleic acids leakage, and electrical conductivity, along with scanning and transmission electron microscopy measurements, confirmed the damaging effects of HNK on the cell wall and membrane of MRSA. Intake of sugar, protein production, molecular docking , and dynamic simulations revealed that HNK interfered with the transport of mannose-based carbohydrates by binding to VAL196, ILE291, ALA293, PRO294, and MET214 on the mannose transporter (ManP) in the phosphotransferase system, and thereby inhibited the carbohydrate metabolism of MRSA. These results indicated that HNK prevented MRSA from using carbohydrates by cutting off its nutritional process, and ultimately caused bacterial rupture and lysis. Therefore, ManP may be a potential target for HNK to combat MRSA infections.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2023
·
IF 10.9
Na Liang,Zixu Yin,Jingyun Guo,Wanying Fang,Qi Wang,Ge Tian,Dong Zhang,Huijuan Yue,Shouhua Feng
Exploring new strategies to incorporate the concepts of green chemistry and defect engineering into new architectures to address the problem of severe electromagnetic wave (EMW) pollution is challenging. In this work, by using a by-product of catalytic conversion of biomass as a carbon source, we prepare two-dimensional (2D) Co 9 S 8 -coated humins-derived carbon nanomaterials with a yolk-shell structure. The EMW attenuation ability is finally optimized by controlling the morphology and defects of the composite material through the pyrolysis temperature. As expected, with the synergy of conductive loss/interfacial polarization and defects regulating, the composite material achieves a reflection loss (RL) value of −51.4 dB in Ku-band and the effective absorption bandwidth (EAB) can be as high as 5.92 GHz (12.08–18.00 GHz) at 1.8 mm ultra-thin coating thickness. A strong absorption capacity in the low-frequency C-band (6.4 GHz) reaches RL value of −49.9 dB and a thickness of 3.50 mm, which grants it the characteristic of convertible microwave absorption from high frequency to low frequency. Taking account of the facile synthesis and tunable absorption properties, the encouraging findings shed light on exploring waste-turned-microwave absorbents with promising practical applications.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Zhiwei Zhou,Yaohua Yan,Xing Li,Fanxi Zeng,Senlin Shao
Nanofiltration (NF) is an effective method for removing trace organic compounds (TrOCs) from drinking and reused water. However, chemical cleaning of NF membranes may impact their properties, leading to a decrease in TrOCs rejection. To better understand the mechanisms behind changes in membrane rejection after chemical cleaning, we investigated the changes in surface properties and TrOCs rejection of two commercial membranes (NF270 and NF90) after urea/ hydrochloric acid (HCl) cleaning, and compared them to conventional sodium hydroxide (NaOH)/HCl cleaning. The findings indicated that urea cleaning increased the membrane pore size and hydrophilicity while marginally affecting membrane crosslinking and zeta potential. Possibly due to the thicker and denser active layer of NF90, neither urea-based nor NaOH-based cleaning had a significant impact on its TrOCs rejection and permeability. In contrast, NF270 was more susceptible to chemical cleaning, and urea-based cleaning had less impact on its TrOCs rejection than NaOH-based cleaning. Although urea cleaning increased the pore size of the NF270 membrane, its pore size recovered well after HCl cleaning, resulting in good recovery of the rejection of hydrophilic neutral TrOCs. Moreover, urea cleaning had limited effects on the electrical properties of the NF270 and might enhance the hydrophilicity of the membrane, resulting in full recovery of the rejection of hydrophobic and negatively charged TrOCs after urea/HCl cleaning.
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Food Bioscience
·
2023
·
IF 5.2
Linlin Jia,Xiaoqiang Peng,Zeyuan Deng,Bing Zhang,Hongyan Li
Moringa oleifera Lam. is an edible plant . In this experiment, three kinds of small molecular weight Polysaccharides (MRP-D, MRP-P, MRP-H) were extracted from Dai-Jia ecology, Pkm-3 and Hong-he 1 Moringa oleifera root that were cultivated in three regions of Yunnan Province. The Moringa oleifera root Polysaccharides (MRPs) were identified to contain β-(1 → 3)-Glucose structure, without triple-helical conformation. In vitro fermentation experiment showed the MRPs increased the content of short-chain fatty acids (3–5-fold) and the abundance of beneficial microflora such as Bacteroidetes and decreased the abundance of harmful microflora such as Proteobacteria at the same time. The composition and effects on the human intestinal microflora of MRPs were different, indicating that the planting regions would affect the composition and function of the bioactives. These results provide an experimental basis for the industrial layout of Moringa oleifera and the application of MRPs in food processing.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Heng-Da Wang,Yu-Qing Zhang
Silk sericin (SS) with various biological activities has limited solubility in water and unpleasant pupal odor, which hinder its wide application in functional foods. Here, a high-yield Maillard reaction (MR) product, glycated sericin, was obtained at a concentration of 60–10 mg/mL with 18–30 mg/mL reducing sugar in a pH 10.0 aqueous solution at 90 °C for 6 h. Xylose had the highest reaction efficiency and the most obvious browning among the seven reducing sugars, followed by arabinose sugar and glucose. No matter what kind of sericin and what kind of reducing sugar, the solubility of the glycated sericin in water increased 2–3 times, and the antioxidant capacity increased 3.5–5.5 times. In particular, glycation of the sericin eliminated the pupal odor of SS and replaced it with a slightly sweet mellow fragrance. Therefore, sericin glycation opens up broad application as a health food, functional food, or food additive.
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Journal of Energy Chemistry
·
2022
·
IF 13.599
Fuyan Kang,Cai Shi,Yeling Zhu,Malin Eqi,Junming Shi,Min Teng,Zhanhua Huang,Chuanling Si,Feng Jiang,Jinguang Hu
The global commitment to pivoting to sustainable energy and products calls for technology development to utilize solar energy for hydrogen (H 2 ) and value-added chemicals production by biomass photoreforming. Herein, a novel dual-functional marigold-like Zn x Cd 1 -x S homojunction has been the production of lactic acid with high-yield and H 2 with high-efficiency by selective glucose photoreforming. The optimized Zn 0.3 Cd 0.7 S exhibits outstanding H 2 generation (13.64 mmol h −1 g −1 ), glucose conversion (96.40%), and lactic acid yield (76.80%), over 272.80 and 19.21 times higher than that of bare ZnS (0.05 mmol h −1 g −1 ) and CdS (0.71 mmol h −1 g −1 ) in H 2 generation, respectively. The marigold-like morphology provides abundant active sites and sufficient substrates accessibility for the photocatalyst, while the specific role of the homojunction formed by hexagonal wurtzite (WZ) and cubic zinc blende (ZB) in photoreforming biomass has been demonstrated by density functional theory (DFT) calculations. Glucose is converted to lactic acid on the WZ surface of Zn 0.3 Cd 0.7 S via the photoactive species O 2 − , while the H 2 is evolved from protons (H + ) in H 2 O on the ZB surface of Zn 0.3 Cd 0.7 S. This work paves a promising road for the production of sustainable energy and products by integrating photocatalysis and biorefine.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 4
·1974-1980
·
IF 4.125
Hengtao Tang,Zhi Chen,Yu Shao,Xin Ju,Liangzhi Li
BACKGROUND The fermentation valorization of two main lignocellulosic monosaccharides, glucose and xylose, is extensively developed; however, it is restricted by limited yield and process complexity. An in vitro enzymatic cascade reaction can be an alternative approach. RESULTS In this study, a three-stage, five-enzyme cascade was developed to convert pretreated biomass to valuable chemicals. First, a ribose-5-phosphate isomerase B mutant isomerized xylose to d -xylulose with high substrate specificity, and a d -arabinose dehydrogenase continued to reduce d -xylulose to d -arabitol. Simultaneously, glucose was utilized for the coenzyme regeneration catalyzed by a glucose dehydrogenase, generating useful gluconic acid and achieving 73% of total conversion rate after 36 h. Then, six kinds of pretreated biomass lignocellulose were hydrolyzed by cellulase and hemicellulase, and corn cob was identified as the initial substrate for providing the highest monosaccharide content. A 65% conversion rate of the lignocellulosic xylose was obtained after 24 h. CONCLUSIONS This study presents a proof of concept to convert main lignocellulosic monosaccharides systematically by an enzymatic cascade at stoichiometric ratio. © 2022 Society of Chemical Industry.
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