ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yi Chen,Xiaowen Jiang,Junnan Wang,Zhengxiu Wu,Yuchen Wu,Zhonghua Ni,Hong Yi,Rongsheng Lu
The clinical challenge of high-accuracy blood glucose detection schemes is to overcome the detection error caused by the background interferences in different individuals. H2O2 as the specific product of glucose oxidation can be involved in the Fe2+/Fe3+ conversion and detected by the time-domain nuclear magnetic resonance (TD-NMR) method sensitively. But, in clinical applications, the oxidation of Fe2+ is susceptible to the complex sample substrates. In this work, we sorted out two kinds of possible interference mechanisms of Fe2+ oxidation in the NMR blood glucose detection method and proposed a feasible scheme that uses sorbitol to weaken the adverse effects of interference. We found that sorbitol-mediated Fe2+ can greatly enhance the sensitivity of the T2 value to H2O2. The chain reaction caused by sorbitol can significantly amplify the efficiency of Fe2+ oxidation at the same concentration of H2O2. Thereby, we can achieve the higher dilution multiple of serum samples to reduce the amount of interfering substances involved in the Fe2+/Fe3+ conversion. We justified the accuracy and availability of our method by successfully detecting and confirming the correlation between the T2 decrease and glucose concentration of the serum samples collected from 16 subjects. The sorbitol–Fe2+ glucose detection method with high sensitivity can be further combined with miniature NMR analyzers to satisfy the calibration requirements of glucose monitoring in diabetic patients instead of frequent medical visits.
研究领域:
医学诊断
·
生物化学
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Tao Liao,Junping Liu,Yuefang Sun,Liqiang Zou,Lei Zhou,Chengmei Liu,Netsanet Shiferaw Terefe,Wei Liu
The inhibitory effects of ascorbic, citric, ferulic and salicylic acid on pear polyphenol oxidase (PPO) were investigated in model and food system. All four organic acids showed strong inhibition effect in model system. However, compared to model system, inhibitory effect of PPO in pear puree was quite different. Ascorbic acid showed a much weaker effect in pear puree compared to the model system. Similarly, ferulic and salicylic acid had limited inhibitory effect in pear puree. Interestingly, the inhibition of PPO activity was not correlated to browning control in pear puree. Ascorbic acid in low concentration was not effective in inhibiting PPO activity in pear puree but exhibited an excellent anti-browning effect. The study indicates that the level of PPO activity is not always a good indicator of susceptibility to browning in foods. Overall, ascorbic acid showed the strongest inhibition of browning and increased the total phenolic content of pear puree.
研究领域:
食品科学
·
酶学
·
有机化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROTECTION
·
2015
·
IF 1.849
Ruiyu Zhu,Ting Yu,Shuanghuan Guo,Hao Hu,Xiaodong Zheng,Petr Karlovsky
The effect of a strain of marine yeast Rhodosporidium paludigenum on postharvest blue mold and patulin accumulation in apples and pears stored at 23°C was evaluated. The occurrence and severity of apple and pear decay caused by Penicillium expansum were significantly inhibited by R. paludigenum. However, the application of the yeast at a high concentration (10 8 cells per ml) enhanced patulin accumulation after 7 days of storage; the amount of patulin increased 24.2 times and 12.6 times compared to the controls in infected apples and pears, respectively. However, R. paludigenum reduced the patulin concentration in the growth medium by both biological degradation and physical adsorption. Optimal in vitro patulin reduction was observed at 30°C and at pH 6.0. R. paludigenum incubated at 28°C was tolerant to patulin at concentrations up to 100 mg/liter. In conclusion, R. paludigenum was able to control postharvest decay in apples and pears and to remove patulin in vitro effectively. However, because the yeast induced patulin accumulation in fruit, the assessment of mycotoxin content after biological treatments in postharvest decay control is important. R. paludigenum may also be a promising source of gene(s) and enzyme(s) for patulin degradation and may be a tool to decrease patulin contamination in commercial fruit-derived products.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEURON
·
2024
·
IF 14.7
Shunchang Fang,Zhengyi Luo,Zicheng Wei,Yuxin Qin,Jieyan Zheng,Hongyang Zhang,Jianhua Jin,Jiali Li,Chenjian Miao,Shana Yang,Yonglin Li,Zirui Liang,Xiao-Dan Yu,Xiao Min Zhang,Wei Xiong,Hongying Zhu,Wen-Biao Gan,Lianyan Huang,Boxing Li
Recognizing the affective states of social counterparts and responding appropriately fosters successful social interactions. However, little is known about how the affective states are expressed and perceived and how they influence social decisions. Here, we show that male and female mice emit distinct olfactory cues after experiencing distress. These cues activate distinct neural circuits in the piriform cortex (PiC) and evoke sexually dimorphic empathic behaviors in observers. Specifically, the PiC → PrL pathway is activated in female observers, inducing a social preference for the distressed counterpart. Conversely, the PiC → MeA pathway is activated in male observers, evoking excessive self-grooming behaviors. These pathways originate from non-overlapping PiC neuron populations with distinct gene expression signatures regulated by transcription factors and sex hormones. Our study unveils how internal states of social counterparts are processed through sexually dimorphic mechanisms at the molecular, cellular, and circuit levels and offers insights into the neural mechanisms underpinning sex differences in higher brain functions.
研究领域:
神经科学
·
行为科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Hui Jiang,Fei Wu,Xi Jiang,Yun-Feng Pu,Li-Rong Shen,Cui-Yun Wu,Hong-Jin Bai
Pear is one of the most popular fruits in the world. With the fruit ripening, a series of physiological changes have taken place in fragrant pear, but up to now, the research on the metabolism and biological activity of phenolic compounds in different growth stages of fragrant pear is still lacking. In this study, four kinds of Xinjiang pears were selected as research objects, and the changes of phenolic content, antioxidant capacity, cell protection and whitening activity during fruit development were analyzed. The results showed that the phenolic content and antioxidant capacity of four pear varieties presented a decreasing trend throughout the developmental stages. The phenolic content and antioxidant activity of the four pears in the young fruit stage were the highest, and the active ingredients of the Nanguo pear were higher than the other three pear fruits. Pear extract could protect cells by eliminating excessive ROS in cells, especially in young fruit stage. The western blot results showed that the extract of fragrant pear in the young fruit stage could inhibit the expression of TYR, TYR1 and MITF in B16 cells, and it was speculated that the extract of fragrant pear in the young fruit stage might have good whitening activity. Therefore, the findings suggest that young pear display a good antioxidant potential and could have a good application prospect in food preservation and health product industry.
研究领域:
食品科学
·
药理学
·
植物生理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2018
·Vol. 12
·期 2
·1266-1273
·
IF 1.181
He Xueming,Fu Xiaping,Li Tingwei,Rao Xiuqin
A spatial frequency domain imaging system (SFDI) was developed to estimate the optical properties of biological samples. The system was calibrated by using a series of self-made solid phantoms which covering a wide range of absorption ( µ
a
) and reduced scattering coefficients ( \({\mu ^{\prime}_s}\) ). The relative errors between the reference and calibrated values were regarded as the evaluation parameters for validation effectiveness of the system, the results showed that the maximum relative errors of µ
a
and \({\mu ^{\prime}_s}\) are 3.92 and 2.39% respectively. Quantitative absorption and scattering maps at the wavelength of 527 nm were obtained for four kinds of pear samples (normal and bruised with three levels of bruised severity with impact energy of 0.025, 0.075 and 0.125 J). The normal pears could be distinguished from the bruised pears by comparing values of coefficient of variation ( CV ) of \({\mu ^{\prime}_s}\) maps, and the correct rates of the discriminant analysis were 100% for non-bruised pears,and 98.33% for bruised pears. Bruises induced using 0.025 J (class 1) were distinguishable from those induced using 0.075 and 0.125 J (class 2) by comparing ratio between mean values of bruised region and normal region of \({\mu ^{\prime}_s}\) maps, the results turned out that the class 1 had the correct rate of 90%, and class 2 had the correct rate of 87.5%. The research showed that SFDI has the potential to be used in the detection of bruises on ‘Crown’ pears and the proposed calibration method lay the foundation for future studies. Future work will be conducted with an emphasis on the acquisition of more spectral information, surface profile correction and the acceleration of detection speed.
研究领域:
生物医学光学
·
食品质量检测
·
成像技术
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Ling Bai,Yanlan Zhang,Cai Zhang,Yuchen Lu,Zhiguo Li,Gang Huang,Bo Meng
A study on the polysorbate 80 stability in various formulation buffers commonly used in biopharmaceuticals was performed, to investigate the excipients influence on polysorbate 80 degradation. Polysorbate 80 is a common excipient in biopharmaceutical products. However, its degradation will potentially impact the drug product quality, and may trigger protein aggregation and particles formation. Due to the heterogeneity of the polysorbates and the mutual effects with other formulation compositions , the study of polysorbate degradation is challenging. Herein, a real-time stability study was designed and performed. The polysorbate 80 degradation trend was monitored by fluorescence micelle-based assay (FMA), reversed-phase-ultra-performance liquid chromatography-evaporative light scattering detector (RP-UPLC-ELSD) assay, and LC-MS assay. These assays provide orthogonal results to reveal both the micelle-forming capability and the compositional changes of polysorbate 80 in different buffer systems. The degradation occurred after a period of storage under 25 °C in different trend, which indicates the excipients could impact the degradation kinetics. Upon comparison, the degradation is prone to happen in histidine buffer than in acetate, phosphate or citrate buffers. LC-MS confirms oxidation as an independent degradation pathway with detection of the oxidative aldehyde. Thus, it is necessary to pay more attention to the excipients selection and their potential impact on polysorbate 80 stability to achieve longer shelf life for the biopharmaceuticals. Besides, the protective roles of several additives were figured out, which could be applied as potential industrial solutions to the polysorbate 80 degradation issues.
研究领域:
生物制药
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2025
·
IF 5.4
Dan Li,Youbing Li,Yu Zhang,Yunsheng Xu,Xianming Zhang,Minna Hakkarainen
Production of high-performance polyesters with tailored degradability remains a challenge. Here, a series of poly(ethylene-co-isosorbide terephthalate) (PEIT) copolyesters were synthesized by varying the isosorbide (IS) content (0–20 mol %) using tetrabutyl titanate (TBT) as the catalyst. By variation of the IS content, the thermal, mechanical, and optical properties of the copolyesters were effectively tailored. As the IS content increased, the Tg was raised from 80 to 101 °C, and the tensile strength from 58.8 to 68.7 MPa. Moreover, excellent transparency was maintained (up to 90% light transmittance). Interestingly, the susceptibility to hydrolytic degradation was significantly enhanced by the incorporation of IS, with PEIT-20 showing approximately 3.5 times higher weight loss compared to PET after 50 days of alkaline degradation in 0.1 M NaOH solution. This outlines an attractive approach for developing high-performance copolyesters with tunable properties and degradation rates, suitable for applications in transparent thermal packaging materials.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2023
·Vol. 23
·期 1
·1-14
·
IF 5.3
Li Guohui,Song Cheng,Manzoor Muhammad Aamir,Li Daoyuan,Cao Yunpeng,Cai Yongping
Background The enzyme phenylalanine ammonia lyase (PAL) controls the transition from primary to secondary metabolism by converting L-phenylalanine (L-Phe) to cinnamic acid. However, the function of PAL in pear plants ( Pyrus bretschneideri ) has not yet been fully elucidated. Results We identified three PAL genes ( PbPAL1 , PbPAL2 and PbPAL3 ) from the pear genome by exploring pear genome databases. The evolutionary tree revealed that three PbPAL s were classified into one group. We expressed PbPAL1 and PbPAL2 recombinant proteins, and the purified PbPAL1 and PbPAL2 proteins showed strict substrate specificity for L-Phe, no activity toward L-Tyr in vitro, and modest changes in kinetics and enzyme characteristics. Furthermore, overexpression of PbAL1 and PbPAL1 -RNAi, respectively, and resulted in significant changes in stone cell and lignin contents in pear fruits. The results of yeast one-hybrid (Y1H) assays that PbWLIM1 could bind to the conserved PAL box in the PbPAL promoter and regulate the transcription level of PbPAL2. Conclusions Our findings not only showed PbPAL’s potential role in lignin biosynthesis but also laid the foundation for future studies on the regulation of lignin synthesis and stone cell development in pear fruit utilizing molecular biology approaches.
研究领域:
植物学
·
分子生物学
✓ 已验证
DOI
高影响因子
Molecular Catalysis
·
2018
·
IF 0
Yuying Zhang,Chuang Li,Zhongtian Du,Xiao Chen,Changhai Liang
Transformation of renewable biomass into chemicals has attracted much attention recently. Efficient catalytic dehydration of biomass-derived sorbitol into isosorbide over solid acid catalysts under moderate reaction conditions is still a technical challenge. A series of silver-exchanged phosphotungstic acid catalysts were prepared by an ion-exchanged process, and dehydration of sorbitol into isosorbide was performed under solvent-free conditions. More than 99% conversion of sorbitol was obtained with 83% selectivity of isosorbide over Ag 1 H 2 PW catalyst at 140 °C within 1 h, which are comparable with that using a homogeneous phosphotungstic acid (HPW) catalyst. The silver-exchanged phosphotungstic acid catalysts were characterized by various techniques such as XRD, FT-IR, Raman, NH 3 -TPD and Py-IR to build a relationship between the catalytic activity and their natures. Ag 1 H 2 PW exhibited much higher activity for catalytic dehydration of sorbitol to isosorbide compared with Ag 2 H 1 PW and Ag 3 PW under the same reaction conditions, which was attributed to the stronger acidity and higher amount of Brönsted acid sites. Moreover, Ag 1 H 2 PW could be easily regenerated by 65% HNO 3 solutions and used repeatedly at least 3 times without obvious loss of catalytic activity.
研究领域:
化学工程
·
催化
·
可再生能源
✓ 已验证
产品关联
DOI
CHINESE JOURNAL OF CHEMISTRY
·
2013
·Vol. 31
·期 8
·1095-1101
·
IF 0.917
Hongyan Bai,Qian Sun,Haiyu Tian,Junhong Qian,Lingyi Zhang,Weibing Zhang
A single boronic acid-based fluorescent probe (compound CSP) for saccharides was designed and synthesized. The probe, with an α , β -unsaturated ketone conjugated into the coumarin fluorophore, was synthesized by 4 steps from the commercial material 4-diethylamino salicylaldehyde. The electron push-pull effect is enhanced with the N , N -diethyl amino as the electron donor and the carbonyl as the electron acceptor. Both the absorption (463 nm) and emission (616 nm) maxima of CSP are in the visible wavelength region with a Stokes shift of about 150 nm, which ensures CSP a potential probe for biological application. Under near physiological conditions, significant fluorescence enhancement of CSP was observed upon the addition of some saccharides, namely, D -sorbitol, D -fructose, D -glucose, D -mannose and D -galactose. The probe showed relatively high sensitivity towards D -fructose and D -sorbitol, and their detection limits were 0.05 mmol/L and 0.1 mmol/L, respectively.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 35
·6165-6171
·
IF 3.756
Xinghui Hao,Jinhui Li,Chenguang Wang,Xinghua Zhao,Xin He,Changquan Calvin Sun
Dimetronidazole (DMZ), an veterinary antibiotic, exhibits poor photostability with only 80.38% potency remaining after exposure to illuminance of 4500 lux for 7 days. The photodegradation is accompanied by obvious color change. A 1 : 1 cocrystal of DMZ with saccharine exhibited profoundly improved photostability with 98.95% of DMZ remaining after 7 days under the same light exposure. Thus, this new cocrystal can be used to develop more stable drug products of DMZ.
研究领域:
药学
·
化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）