NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 33
·15721-15730
·
IF 3.3
Wanyu Song,Peng Sun,Jihong Zhou,Xu Han,Qijun Dai,Fang Chai
The Keggin phosphotungstic acid (PW12) embedded metal–organic framework copper benzene-1,3,5-tricarboxylate (CuBTC), CuBTC@PW12, was fabricated by a facile hydrothermal synthesis technique. With a unique tetrakaidecahedron structure and multiple active sites, the obtained CuBTC@PW12 exhibited peroxidase-mimicking activity, which promoted a novel strategy for qualitative and quantitative detection of hydrogen peroxide (H2O2) and sequential sensing of ascorbic acid (AA) via colorimetric evolution. Notably, with the advantages of strong redox performance of POMs and the unique porous structure of metal organic frameworks (MOFs), as peroxidase mimics, CuBTC@PW12 achieved highly selective and sensitive detection of H2O2via oxidizing TMB to a blue oxidation product (ox-TMB), with a limit of detection of 2.67 μM within the 0.01 to 1.0 mM scale. Concomitantly, the AA can be monitored consecutively by reducing the ox-TMB with fading blue color quantitatively with a limit of detection at 0.15 μM in the range of 0.01–0.10 μM. The recoveries for the determination of AA in human serum were obtained at 94.63–105%. These results manifested that CuBTC@PW12 could be used as a peroxide-mimetic enzyme for the continuous detection of H2O2 and AA with the advantages of simplicity, efficiency, sensitivity and accuracy.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yaxue Jia,Siqi Zhao,Anping Wang,Jing Huang,Jinlan Yang,Li Yang
A target-induced multiregion MNAzyme nanowire system is designed for the ultrasensitive and homogeneous detection of microRNAs (miRNAs). miRNA-21 and miRNA-375 are chosen as analytes, and a miRNA-induced primer exchange reaction (PER) is utilized to construct a long DNA strand with repetitive sequences. This innovative design enables the efficient anchoring of numerous MNAzymes. This unique architecture significantly boosts the effective local concentration of MNAzymes, thereby enhancing the sensitivity and efficiency of miRNA detection. Notably, the limit of detection (LOD) achieved with our target-induced multiregion MNAzyme nanowire approach is over an order of magnitude lower than most other MNAzyme-based methods, while the MNAzyme reaction time is reduced from several hours to 50 min. The method has demonstrated successful applications in quantitatively determining the expression levels of two miRNAs in cell lysates of MCF-7, HeLa and MCF-10 A cells, highlighting its potential for assaying miRNA biomarkers in clinical samples.
研究领域:
分子生物学
·
生物化学
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wusen Zhou,Jiacheng Gong,Shan Lu,Zhuo Li,Yan Long,Xingjun Li,Renfu Li,Xiaoying Shang,Yan Liu,Datao Tu,Zhuo Chen,Xueyuan Chen
As energy currency of life, adenosine triphosphate (ATP) is highly dynamic in living organisms. It is crucial yet challenging to probe intracellular or in-vivo ATP precisely without destruction of the living biological systems. Herein, we have designed novel ATP-responsive ratiometric nanoprobes by employing upconversion nanoparticles (UCNPs) with cypate as antennas. Nuclear magnetic resonance spectroscopy was used to analyze the interfacial interactions among lanthanide nanocrystal, cypate and adenosine phosphates, disclosing the origin of detection specificity. ATP induced interruption of energy transfer pathway in cypate-sensitized UCNPs was further investigated by femtosecond transient absorption and time-resolved photoluminescence spectroscopy. The nanoprobes with dual near-infrared (NIR)-excited ratiometric UCL were successfully applied for quantification of ATP level in living cells under vegetatively growing, starving, and glycolysis-inhibiting states, respectively. Furthermore, the increase of available energy by feeding for zebrafish larvae was confirmed through monitoring the in-vivo ATP variation with the nanoprobes. Such nanoprobes showed great potential in ATP-associated biological research and clinical applications. These findings may gain deep insights into the surface science and photophysics in dye-lanthanide nanocomposites, and open new avenues for the design and bioapplications of lanthanide nanoprobes.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Yaqi Huang,Wei Liu,Tiantian Man,Jinwei Du,Xianli Gong,Fujin Lv,Wenhao Shan,Lu Ding,Ying Wan,Shengyuan Deng
Conventional point-of-care testing (POCT) has limitations in sensitivity with high risks of missed detection or false positive, which restrains its applications for routine outpatient care analysis and early clinical diagnosis. By merits of the cutting-edge quantum precision metrology, this study devised a mini quantum sensor via magnetofluorescent qubit tagging and tunning on core–shelled fluorescent nanodiamond FND@SiO2. Comprehensive characterizations confirmed the formation of FND biolabels, while spectroscopies secured no degradation in spin-state transition after surface modification. A methodical parametrization was deliberated and decided, accomplishing a wide-field modulation depth ≥15% in ∼ zero field, which laid foundation for supersensitive sensing at single-FND resolution. Using viral nucleocapsid protein as a model marker, an ultralow limit of detection (LOD) was obtained by lock-in analysis, outperforming conventional colorimetry and immunofluorescence by > 2000 fold. Multianalyte and affinity assays were also enabled on this platform. Further by resort to artificial-intelligence (AI) augmentation in the Unet-ConvLSTM-Attention architecture, authentic qubit dots were identified by pixelwise survey through pristine qubit queues. Such processing not just improved pronouncedly the probing precision but also achieved deterministic detections down to a single protein in human saliva with an ultimate LOD as much as 7800-times lower than that of colloidal Au approach, which competed with the RT-qPCR threshold and the certified critical value of SIMOA, the gold standard. Hence, by AI-aided digitization on optic qubits, this REASSURED-compliant contraption may promise a next-generation POCT solution with unparalleled sensitivity, speed, and cost-effectiveness, which in whole confers a conclusive proof of the prowess of the burgeoning quantum metrics in biosensing.
研究领域:
生物医学工程
·
量子生物学
·
分子诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yongbin Zhang,Yongfei Huang,Yongkang Yue,Jianbin Chao,Fangjun Huo,Caixia Yin
Because of the high toxicity of hydrazine, the development of ultrasensitive fluorescent probes for hydrazine has been challenging. In this work, an ultrasensitive fluorescent probe based on o -phthalaldehyde has been exploited for hydrazine. The probe was designed based on creating an efficient fluorophore by incorporating hydrazine into o -phthalaldehyde framework via an intermolecular condensation-cyclization reaction. The probe could quickly detect hydrazine and displayed a high sensitivity for hydrazine with a LOD of 1.9 nM (0.06 ppb), which is far below the U.S. Environmental Protection Agency standard (10 ppb). Moreover, the probe was successfully applied to the detection of hydrazine in real water samples and hydrazine vapor.
研究领域:
分析化学
·
环境监测
·
生物传感
·
分子探针
·
毒理学
✓ 已验证
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2019
·Vol. 29
·期 6
·1423-1429
·
IF 1.913
Meng Xianjiao,Cao Duanlin,Hu Zhiyong,Li Zhichun,Han Xinghua,Ma Wenbing
In this study, a BODIPY-based water-soluble fluorescent chemosensor BBP has been synthesized using BODIPY as the fluorescence group and quinoline as the recognition group. BBP can be used for naked eye detection of pH in complete aqueous solution and it shows high specificity in a wide range of cations. The p Ka value is determined to be 2.94 and the fluorescence intensity is linearly related to pH in the range of 2.4–3.6.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2021
·
IF 3.599
Lei Jia,Xiangzhen Chen,Jun Xu,Lina Zhang,Xiyan Dong,Dan Zhao,Ning Bi,Taofeng Zhu,Tongqian Zhao
It is of vital significance to achieve accurate real-time detection of tetracycline (TC). In this research, the ratio fluorescence detection of tetracycline is realized by introducing europium-based aminoclay containing carbon dots with good sensitivity to tetracycline through subsequent surface modification. With the increase of tetracycline concentration, the fluorescence intensity of rare earth europium fluorophore increases continuously, while the fluorescence intensity of CDs is basically unchanged. The CIE chromaticity coordinate confirms that the luminous color of the probe can be adjusted continuously from blue to red. The results exhibit this approach owns the favourable linearity to TC within 0–12.5 μM and 10–25 μM, and the high sensitivity with a low detection limit of 3.133 nM. Moreover, it can entirely satisfy the requirements of field detection because the rapid monitoring time as short as 30 s. The multicolor fluorescence detection strategy is reliable and feasible in the field test of TC in various actual samples. In addition, a TC floating detector based on vertical float structure is designed to achieve the rapid visual detection of TC in simulated flow environment. The superiority of the sensing platform opens up a neo-pathway for point-of-care monitoring of TC residues.
研究领域:
分析化学
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环境监测
·
生物传感器
·
纳米技术
·
食品安全
·
药物分析
·
材料科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Min Li,Liwei Jiao,Min Li,Lihua Zhi
The development of innovative approaches for portable, highly sensitive, and selective detection of mercury ions holds immense value for both biochemical and environmental analysis. In this study, a simple, user-friendly, label-free and cost-effective multimodal point-of-care testing (POCT) platform was established based on in situ-grown gold nanoparticles (Au NPs) embedded in a porous covalent organic polymer (COP-180). This novel platform utilizes a unique combination of pressure, temperature, and colorimetric signals for multi-modal readout to realize precise and specific identification of Hg(II). In an alkaline environment, Hg 2+ triggers the catalase-like activity of Au/COP-180, promoting the decomposition of H 2 O 2 into oxygen (O 2 ), producing a detectable pressure signal. Conversely, in acidic conditions, Hg 2+ enhances the peroxidase-like activity of the Au/COP-180, leading to the generation of reactive oxygen species (•OH) that contribute to colorimetric and photothermal signals. This platform exhibits excellent linearity across all three detection modes, with detection limits of 9 nM for pressure, 27.4 nM for colorimetry, and 0.1 μM for temperature. Furthermore, this approach demonstrated satisfactory results for detecting mercury ions in real water samples. This innovative multimodal POCT strategy provides a robust and operationally simple solution for detecting heavy metal ions with high sensitivity and accuracy, particularly in resource-limited settings.
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jiuhong Zhao,Wenjing Zhu,Yuxuan Mao,Guixia Ling,Peng Zhang
The increased uric acid (UA) level is closely related to many diseases. However, the detection of UA remains great challenges due to the pain of blood testing and the complexity of common methods. Therefore, the detection of UA is of great significance for public health. In this paper, a new method for UA detection is proposed. The swellable hydrogel microneedles (hydrogel MNs) can be used for minimally invasive extraction of skin interstitial fluid (ISF). The colorimetric detection of trace UA in ISF can be successfully achieved by virtue of DNAzyme@MOFs with enhanced peroxidase activity. This study provides a powerful tool for minimally invasive, painless, rapid and convenient detection of UA in clinical applications. This study not only avoids the use of expensive and unstable natural enzymes but also expands the applications of DNAzyme@MOFs in biomedical detection.
研究领域:
医学检测
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
STAR Protocols
·
2023
·
IF 0
Tiantian Feng,Xuran Chen,Shilei Zhao,Meng Cao,Lijuan Feng,Se Shi,Hui Wang,Tao Liu,Yihui Yuan,Ning Wang
Summary Detection of radioactive iodide ions (I – ) is important for protecting human beings from the hazards of radioactive pollution. Herein, we present a protocol for detecting I – using a zirconium(IV)-enhanced strategy. We describe steps for optimizing the I – detection approach, establishing standard curves, and finally applying the approach. The use of zirconium(IV) greatly improves the detection performance and endows this approach with an ultralow detection limit of 0.176 nM together with wide applicability in various aqueous environments. For complete details on the use and execution of this protocol, please refer to Feng et al. (2022). 1
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
PubMed
ELECTROANALYSIS
·
2020
·Vol. 33
·期 4
·947-955
·
IF 2.544
Yanqiu Fu,Jinrui Li,Wei Jin,Zhonggang Liu
In this work, a dual-functional electrochemical sensor has been proposed based on Sn-doped defective Bi 2 S 3 (TDDB) microspheres, which exhibited the excellent electrochemical performance on Pb(II) and H 2 O 2 detection. The TDDB offered a satisfied detection limit of 8.0 nM towards Pb(II) with a sensitivity of 96.7 μA ⋅ μM −1 . As a H 2 O 2 sensor, a high sensitivity of 3540 μA mM −1 cm −2 was obtained in a linear range from 0.45 mM to 10 mM with a detection limit of 10 nM. Moreover, the electrochemical detection of Pb(II) in Taihu Lake and H 2 O 2 in human serum was achieved with high reliability and good recovery.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ke Huang,Rui Dai,Wenqing Deng,Shengjie Guo,Han Deng,Yao Wei,Fuling Zhou,Ying Long,Jun Li,Xin Yuan,Xiaoli Xiong
A novel visual detection method was developed for the determination of zinc (Zn) in whole blood and cells using gold nanoclusters (AuNCs) immobilized paper. The detection of Zn was based on a headspace-solid phase extraction (HS-SPE), visible fluorescence from the AuNCs coated paper was quenched by ZnH 2 generated from hydride generation (HG). The potential mechanism was investigated by using X-ray photoelectron spectroscopy (XPS) and Density Functional Theory (DFT). The selectivity was significantly increased because the zinc hydride was effectively separated from sample matrices by hydride generation. A limit of detection (LOD) of 3 μg L −1 Zn 2+ and a relative standard deviation (RSD, n = 7) of 2% at a concentration of 50 μg L −1 of Zn 2+ were obtained when using a commercial fluorescence spectrophotometer as the detector. In visual detection, as low as 20 μg L −1 of Zn 2+ in biological samples can be easily discriminated from the blank with the naked eye. The proposed method retains several unique advantages, including straightforward, rapid, affordable and equipment-free for visual detection.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）