Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 9
·2563-2569
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IF 6.641
Chao Xia,Mengmeng Cao,Jinfeng Xia,Guohong Zhou,Danyu Jiang,Dafeng Zhang,Jing Wang,Huili Li
Carbon dots (CDs), as a new kind of fluorescent pH nanoprobe, have attracted significant attention due to their favorable optical properties and excellent environmental friendliness. However, a real ratiometric and self-calibrating fluorescent pH nanoprobe based on intrinsic dual-emission CDs (DECDs) still remains scarce. In this study, we reported a kind of intrinsic DECDs for ratiometric fluorescence pH sensing. Interestingly, the two emission bands at 393 and 580 nm from the DECDs simultaneously display strong pH-dependence but with an adverse variation trend. The ratio of the intensity of 393 and 580 nm matches well with an empirical function against pH in the range from 2.2 to 8.0, and further, an excellent linear relationship is presented in an acidic environment with a responsive pH range of 2.2–4.0. Moreover, the rapid response time (<1 min), high sensitivity, good reversibility and pinpoint accuracy (±1.0%) for pH sensing reveals that the as-prepared DECDs can act as excellent ratiometric luminescent nanoprobes for pH detection. We hope that this study will offer new possibilities for expanding the applications of luminescent CDs in chemical sensing, biomedicine and food safety.
研究领域:
化学
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生物医学
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食品安全
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高影响因子
Laser & Photonics Reviews
·
2024
·Vol. 18
·期 12
·2400786
·
IF 9.8
Wang Wang,Jinqiu Yang,Bolin Ma,Haoyu Zhu,Yaqi Zhu,Yuexi Yi,Xiaodan Li,Qiqing Li,Haoran Chen,Bin Li,Xianggui Kong,Yulei Chang
Fluorescence/luminescence bioimaging, utilizing rare-earth doped nanocrystals (RENCs) within the NIR-IIb (1500–1700 nm) sub-window, has drawn interest for its deep penetration depth and superior resolution. Herein, Er 3+ /Yb 3+ couple is rationally designed in topologically arranged RENCs that facilitate a boosted 1530 nm emission of Er 3+ via premeditated Yb 3+ -mediated energy transfer processes under 808 nm excitation. To maximize this modulation effect for boosting 1530 nm emission, a regional active ions concentration and spatial regulation strategy is explored in the optimized concentration and core–shell–shell structure (NaErF 4 :20%Yb@NaYbF 4 :20%Er@NaYF 4 with a quantum yield of 16.46% in NIR-IIb region). Leveraging the superior emission and multi-wavelength excitation characteristics of the as-obtained nanocrystals, a NIR-IIb-based ratiometric fluorescence imaging measurement is achieved under 808 and 980 nm excitation, which cannot only show the 2D distribution of probes but also provide spatial depth information (range from 0 to 2.8 mm with a tissue depth resolution of 66 µm). The findings hold significant potential for advancing both vascular science and clinical medicine.
研究领域:
生物医学工程
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材料科学
·
光学成像
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ziyi Qin,Jinju Xu,Yuanxin Cao,Chunhui Liao,Tian Lan,Shuyun Shi
Highly sensitive monitoring of pesticides has garnered attention for food safety. Here, a ratiometric fluorescent probe (AG-CDs-Al 3+ /CdTe QDs) was fabricated for specific and sensitive sensing of glyphosate. Surface modifications revealed the chelation-enhanced fluorescence process between the surface -OH of AG-CDs and Al 3+ . Positively charged green-emission AG-CDs-Al 3+ assembled with negatively charged red-emission CdTe QDs, and photoinduced electron transfer (PET) occurred from CdTe QDs to AG CDs-Al 3+ . Glyphosate specifically coordinated with Al 3+ , causing remarkable variations in F 500 /F 630 (linear range, 0.2–4.0 μM; detection limit, 5.7 nM) and a distinguishable green-yellow-red color change on the paper platform (linear range, 0.5–8.0 μM; detection limit, 26 nM). Furthermore, for real sample analysis, the sensor has the advantages of fast response (5 min), excellent accuracy (spiked recoveries, 95.0–105.0 %), high precision (relative standard deviation, < 3.33 %), and superior specificity, which endowed the designed sensing platform with great potential practicality in the field of food analysis.
研究领域:
食品安全
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分析化学
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环境科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhiyu Xue,De Ning,Kaihong Jia,Hao Liu,Yong Xiang,Jinlong Cao,Junxian Chen,Yeshuang Zhong,Xinyu Wang,Zhen Zhang
Carbon dots (CQD) have received significant attention as a novel ratiometric fluorescent pH nanoprobe, owing to their favorable optical properties and excellent biocompatibility. Despite their appealing features, the precise mechanism behind the pH-sensitive photoluminescence of CQDs remains to be fully understood. This study endeavors to unravel the mechanism underlying the pH-responsive ratiometric fluorescence in dual-emission CQDs, synthesized through a one-step hydrothermal method using o-phenylenediamine and oxalic acid as precursors. The resultant CQDs exhibit inherent dual-emission at wavelengths of 383 nm and 566 nm, with the ratiometric fluorescence response tailored by the ratio of precursors, providing a robust tool for pH sensing across a range of 2 to 6. Detailed characterizations, including chemical, morphological, and optical analyses, alongside theoretical insights from time-dependent density functional theory (TD-DFT), elucidate the mechanism underlying the pH-dependent luminescence, attributed to the electron cloud transmission between amide and adjacent carboxyl groups on the CQD surface. The superior performance of these CQDs in real-time pH monitoring is demonstrated through their application in glucose oxidase-catalyzed reactions, showcasing their potential as efficient, reliable nanoprobes for biomedical research and diagnostic applications.
研究领域:
材料科学
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生物医学工程
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化学
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 17
·175604
·
IF 3.874
Fan Liu,Yuan Jiang,HaiBao Shao,Chunlei Wang,Shuhong Xu,Rong Zhang
Precise control of the structure and bonds of doped carbon dots (CDs) is important, so that their fluorescence can be tuned as desired. Up until now, there has been a lack of effective ways to control the bonds of doped CDs. In this article, we show that the fluorescence of B/N-doped carbon dots (B/N-CDs) can be precisely tuned just by controlling their precursors' pH values. The prepared B/N-CDs exhibit two emission bands, including one emission peak at around 450 nm due to the defect state caused by low sp2 hybridization of carbon atoms, as well as another emission peak at around 360 nm caused by the B-N bond. The results for the ratio of the maximum intensity of the two emission peaks above show a linear relationship. Meanwhile, the role of the pH value of the precursors on the luminescence of B/N-CDs is deeply studied. On the one hand, the pH value affects the bonding process of CDs. N-H bonds tend to form at a low pH value, while more competitive B-N bonds exist at much higher pH value, leading to the pH-dependent intensity of the 360 nm emission band. On the other hand, a high pH value causes higher crystallinity, thus suppressing defect-state fluorescence at 450 nm. The dual effects of pH lead to precisely controlled dual-emission intensities as well as ratiometric fluorescence.
研究领域:
材料科学
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化学
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DOI
PubMed
高影响因子
ANALYST
·
2015
·Vol. 141
·期 3
·1098-1104
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IF 4.107
Xiang Han,Lei Zhao,Dong-En Wang,Juan Xu,Longlong Zhang,Mao-Sen Yuan,Qin Tu,Jinyi Wang
In this paper, we designed and synthesized a novel TBET-based ratiometric fluorescent chemodosimeter, RH-Au, for Au3+. It was found that the probe RH-Au displayed highly selective, sensitive and naked-eye detection upon the addition of Au3+. The probe RH-Au can be used in the pH range 6.0–7.5 and the detection limit was determined to be as low as 2.91 nM (0.57 ppb). We also demonstrated a successful application of imaging Au3+ in living cells using RH-Au.
研究领域:
分析化学
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生物医学
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PubMed
高影响因子
RSC Advances
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2023
·Vol. 13
·期 34
·23812-23817
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IF 3.9
Gao Liang,Zheng Minghao,Liu Haiyi,Xiao Jun,Gong Tianhao,Liu Kunming,Li Juanhua,Liu Jinbiao
A 2-(2′-hydroxyphenyl)benzimidazole (HBI)-based ratiometric fluorescent probe, known as BTEP, was synthesized using 5-bromosalicylaldehyde as the raw material via Sonogashira coupling and condensation reaction. This probe was designed for rapid detection of boron trifluoride solutions and gases. The N and O coordination atoms in the probe undergo a boron difluoride addition with BF3, which affects the process of excited state intramolecular proton transfer (ESIPT) leading to a blue shift of fluorescence emission. Obvious changes in the fluorescence signal can be observed within 60 seconds. The introduction of an acetylene trimethylsilane fragment increases the conjugate plane and is beneficial to improving the selectivity of the probe. The I408/I479 fluorescence ratio of the probe displays a linear relationship with the concentration of BF3 in the range of 5–50 μM, with a detection of limit as low as 69.5 nM. Furthermore, the probe demonstrates specific and selective recognition of BF3 among eight common interference substances. Test strips prepared using BTEP have the capability of real-time naked-eye detection of trace BF3 gas.
研究领域:
分析化学
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dan Chang,Hu Shi,Jianyang Feng,Yang Liu,Yanan Yan,Lihong Shi,Yongxing Yang,Shaomin Shuang,Chuan Dong
A fully integrated handheld sensor with a sample-to-answer fashion for target determination is essential for food industry and environmental science. However, the most important issue for existing sensors is still time-consuming and lacks user-friendly on-site monitoring capabilities. To address this issue, we first design a portable artificial intelligence-driven handheld sensor to achieve in-situ monitoring of amoxicillin (AMO). The intelligent platform consists of the high-throughput paper sensor loaded with carbon dots (CDs), a custom-built 3D-printed attachment, and a smartphone with machine learning (ML) function. Note that the introduction of AMO into CDs system triggers a hydrogen bond reaction, resulting in a ratiometric signal variation accompanied by the fluorescence (FL) color changes from yellow to blue. In real-time mode, an open-source ML algorithm is deployed in a self-programming applet from smartphone to directly output AMO concentration of samples, allowing for intelligent on-site analysis. Importantly, the system enables rapid detection of AMO in a wide range of 5 μM to 1000 μM, with an acceptable detection limit of 2.8 μM. Moreover, we verify the integrated system can be used to quantify AMO in real samples. This proposed ML-driven sample-to-answer sensing strategy will inspire the development of intelligent sensors.
研究领域:
生物传感
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分析化学
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环境科学
·
食品工业
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Cong Dai,Ying Xu,Bo Liu,Biao Gu
Organic cages with long-wavelength emission and ratiometric signal are pivotal but still challenging for their sensing application. Probes often suffer from single signal and poor water solubility, which are detrimental to hypochlorous acid (HClO) sensing in aqueous solution. Here, we report the fabrication of organic cages with long-wavelength dual emission for ratiometric fluorescent sensing of HClO. The nanosized organic cages (OCs-1 and OCs-2, derived from the reaction of 1,3,5-tris(aminomethyl)benzene) and 2- or 4-hydroxyisophthalaldehyde) showed robust stability across varying pH. Both OCs-1 and OCs-2 exhibited pH-responsive dual emission attributed to hydroxy-1,3-diiminobenzene group, facilitated by the enol-keto tautomerism and constrained molecular motion within the capsule topological structure. Owing to the redox reaction between imine bond of OCs-2 and HClO, the dismantling of cage-like structure led to significant quenching of emission at 620 nm and enhancement of emission at 500 nm. Based on the ratiometric signal of OCs-2, a highly effective sensing method for HClO was established. The proposed method gave the linearity range of 0.5–3 μM, the limit of detection (3 s ) of 4 nM, and remarkable precision with the relative standard deviation of 0.58 % for 10 replicate determinations of 1.5 μM HClO. The recoveries of spiked HClO in domestic water and disinfectant samples ranged from 94.09 % to 104.24 %.
研究领域:
分析化学
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生物医学工程
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Yanan Yan,Lin Li,Huilin Zhang,Fangfang Du,Yating Meng,Shaomin Shuang,Ruibing Wang,Shengmei Song,Chuan Dong
The B,N dual-doped carbon dots (B,N-CDs) for ratiometric fluorescence detection the morin were prepared from sodium tetraborate and polyethyleneimine through the single-step hydrothermal method . The B,N-CDs exhibited the optimum excitation and emission wavelength at 340 nm and 467 nm, respectively. Interestingly, the intensities of emission peak at 467 nm of B,N-CDs reduced meanwhile a new peak emerged at 560 nm with the continuous addition of morin, which revealed the ratio fluorescence characteristic between F 560nm /F 467nm and morin concentration with the linearity range and detection limit of 14.5–32.5 μmol/L and 0.3 μmol/L (S/N = 3), respectively. The interference of common antibiotics and remedies could be ignored when the concentration of morin was detected by the B,N-CDs, which demonstrating the outstanding selectivity. Furthermore, the proposed fluorescence method is used to detect morin in urine with recoveries are 99.8–104.5%. The results of this research indicate the feasibility and practicality of B,N-CDs as an effective fluorescent probe for the determination of morin.
研究领域:
分析化学
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材料科学
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药物分析
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yong Han,Weixia Yang,Xueli Luo,Xie He,Ying Yu,Chunhua Li,Wenzhi Tang,Tianli Yue,Zhonghong Li
Copper ion (Cu2+)-triggered carbon dots (CDs/Cu) with dual emissions were utilized to develop a ratiometric fluorescence sensor for ultrasensitive detection of inert thiophanate-methyl (TM). TM could be recognized by CDs/Cu through π–π stacking and could chelate Cu2+ of CDs/Cu through metal ion coordination, inducing synchronous fluoresent quenching of the dual emission of CDs/Cu based on the excited state intramolecular proton transfer and the ligand-to-metal charge transfer. The fluorescence ratio of CDs/Cu (F416/F481) linearly responded in a TM concentration of 0.10–20.00 μmol/L with an ultralow limit of detection of 2.90 × 10–6 μmol/L. A synchronous response of the ratiometric sensor enhanced the specificity toward TM and presented remarkable capability of anti-interference in complex matrices. The sensor exhibited satifactory accuracy and precision for practical applications with recoveries of 88.33–101.09% and relative standard deviations of 1.61–5.06%, demonstrating an ultrasensitive ratiometric fluorescent nanosensor for detecting pesticides residues in complex matrices.
研究领域:
分析化学
·
环境科学
·
食品安全
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Zhu Han,Danyang Nan,Huan Yang,Qianqian Sun,Shuang Pan,Hui Liu,Xiaoli Hu
A novel method was established for the ratiometric fluorescence detection of Cu 2+ and glutathione (GSH) by carbon quantum dots (CQDs), and it was fabricated through one-pot facile hydrothermal treatment using o-phenylenediamine (OPD) and citric acid as precursors. Based on the selective oxidation reaction of OPD with Cu 2+ , the detection strategy of Cu 2+ was proposed using ratiometric fluorescence probe. The oxidation production (2,3-diaminophenazine) of OPD, obtained through the oxidation reaction of OPD and Cu 2+ , not only emerged a new emission peak at 562 nm, but also quenched the fluorescence of CQDs with maximum emission at 446 nm. The mechanism was Förster resonance energy transfer (FRET) between CQDs and 2,3-diaminophenazine (oxOPD). Furthermore, the oxidation reaction between Cu 2+ and OPD could be inhibited when added GSH into the solution, which could prevent the fluorescence of CQDs being quenched. The sensing system showed high sensitivity toward Cu 2+ and GSH in a range of 0.25–10.0 μmol L −1 and 1.0–80.0 μmol L −1 with a detection limit 0.076 μmol L −1 and 0.30 μmol L −1 , respectively. Besides, the proposed method could apply to efficient quantification of Cu 2+ and GSH in practical samples.
研究领域:
分析化学
·
生物医学
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高影响因子
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