查找使用阿拉丁产品的科研文献

Fin Cells as a Promising Seed Cell Source for Sustainable Fish Meat Cultivation

Zongyun Du,Jihui Lao,Yuyan Jiang,Jingyu Liu,Shili Liu,Jianbo Zheng,Fei Li,Yongyi Jia,Zhimin Gu,Jun Chen,Xiao Huang

Cell-cultured meat production relies on stable, proliferative seed cells, commonly sourced from muscle satellite cells (MuSCs) and adipose-derived mesenchymal stem cells (AD-MSCs). However, establishing such cell lines in fish species remains technically challenging. While pluripotent stem cells (e.g., ESCs/MSCs) offer alternatives, their differentiation efficiency and predictability are limited. Here, we developed TCCF2022, a novel caudal fin-derived cell line from Topmouth culter (Culter alburnus), which expresses pluripotency markers (AP,Oct4,Sox2,Klf4, andNanog) and aggregated growth to form 3D spheroids. Forskolin supplementation enhanced pluripotency maintenance. The presence of adipogenic and myogenic lineage cells within the 3D spheroids was confirmed, demonstrating their potential as seed cells for cell-cultured meat. Using a small-molecule cocktail 5LRCF (5-Azacytidine, LY411575, RepSox, CHIR99021, and Forskolin), we successfully differentiated TCCF2022 cells into functional myotubes. Additionally, we established a method to induce the differentiation of TCCF2022 cells into adipocytes simultaneously. Thus, the TCCF2022 cell line can be used to improve muscle fiber formation and lipid composition, potentially enhancing the nutritional profile and flavor of cultured fish meat.

Zwitterionic Poly(sulfobetaine methacrylate) Brushes Functionalized Threads for DNA Extraction from Complex Cell Lysates

Xianlong Shi,Liang Wu,Ke Ning,Xinmei Li,Lingke Feng,Yirong Chen,Ling Yu

Thread-based analytical devices are low-cost, portable, and easy to use, making them ideal for detecting various biomolecules like glucose and DNA with minimal sample requirements, while also offering environmental benefits through their biodegradability. This study explores the potential of zwitterionic poly(sulfobetaine methacrylate) brushes modified cotton thread (PSBMA@threads) as an innovative substitute for DNA solid-phase extraction. The PSBMA polymer brushes were synthesized on cotton threads via surface-initiated atom transfer radical polymerization (SI-ATRP). The usability of the PSBMA@threads for DNA extraction from cell lysates containing cell debris, proteins, and detergents was evaluated. Characterization using SEM, FTIR, and EDS confirmed the successful functionalization with PSBMA polymer brushes. The antifouling properties of PSBMA@threads, including resistance to non-specific protein adsorption and underwater oil repellency, were assessed. The results demonstrated selective DNA capture from protein and lipid-rich lysates. Optimized extraction parameters improved DNA yield, enabling efficient extraction from tumor cells, which successfully underwent PCR amplification. Comparative experiments with commercial silica membrane-based columns revealed that PSBMA@threads exhibited comparable DNA extraction capability. The PSBMA@threads maintained extraction capability after six months of ambient storage, highlighting its stability and cost-effectiveness for nucleic acid isolation in analytical applications.

Gradient-Driven Sandwich-like Nanofibrous Membrane for High-Flux Antifouling Emulsion Separation

Xinying Zhang,Jialu Zhang,Bin Lu,Xiangfeng Huang,Xinyuan Peng,Jia Liu,Chen Cai,Kaiming Peng

Membrane separation for nanoemulsion treatment offers advantages including superior effluent quality and low resource consumption. However, it faces challenges of low flux and membrane fouling. To address these, we fabricated a sandwich-like spindle-knot nanofibrous membrane (SSK-NM) to enhance water permeation and mitigate fouling. The SSK-NM features a thin fiber layer as the skin, a spindle-knot layer in the middle, and a thick fiber layer as the base. Fabricated via controlled spinning solution concentration and deposition sequence, the SSK-NM incorporates three gradients including hydrophilic wettability (water contact angle from 38.6° to 21.3°), inverse pore size (from 3.1μm to 0.604μm), and curvature. For oil-in-water emulsions, a 6μm thick skin layer dramatically boosted flux by up to 153%, achieving a 39,912 LMH/bar with >99% oil rejection. Concurrently, this skin layer shifted dominant fouling model away from cake filtration, reducing fouling indices by 41.9%. Rapid vertical water droplet penetration, evidenced by a 45% increase in penetration rate compared to a skin layer-free membrane, was driven by a substantial positive Laplace pressure difference (∼369.53 kPa) generated through the combined pore size and wettability gradients. Crucially, the 6μm skin layer maintained optimal hydrophilicity without significant loss of anti-fouling, shown by the maximum contrast in water adhesion force and oil adhesion force (ΔF=96.2 μN). Thinner layers were insufficient to drive droplets, while thicker layers formed dense surfaces prone to pore blocking. The SSK-NM exhibits exceptional separation efficiency and universality across diverse emulsions and industrial oily wastewater, offering a streamlined, single-material strategy for designing high-flux, antifouling membranes.

Flexible Hierarchical-Porous TiO2/ZnO@Carbon Nanofiber Membranes with Synergistic Oil–Water Separation and Visible-Light-Driven Self-Cleaning for Sustainable Wastewater Remediation

Zhengyu Ding,Xinnan Zhou,Binjie Xin,Yalong Liu,Xuan Xing,Zheyao Xia,Jun Zhang,Kainan Guo

Oily wastewater and antibiotic residues (e.g., tetracycline hydrochloride, TCH) pose significant threats to aquatic ecosystems and human health. Conventional remediation technologies frequently suffer from low efficiency, secondary pollution, or high energy consumption. Rigid ceramic membranes, once deemed the “gold standard” in separation fields, exhibit inherent drawbacks, including brittleness, low porosity, and severe fouling propensity. To address these challenges, flexible hierarchical-porous carbon nanofiber membranes codoped with TiO2/ZnO heterojunctions (TZCNFMs) were successfully fabricated via electrospinning, preoxidation, and carbonization processes. Research findings demonstrated that the optimized TZCNFM (with a specific TTIP/Zn(OAc)2 mass ratio) exhibited superior comprehensive performance, characterized by a rough surface with nanoprotrusions, a large specific surface area, and favorable tensile strength. This optimized membrane showed strong hydrophobicity and superoleophilicity, enabling high separation efficiency for water-in-oil emulsions. Under visible light irradiation, the constructed TiO2/ZnO heterojunctions in TZCNFM2 formed a staggered band structure, which not only effectively suppressed the recombination of photogenerated electron–hole pairs but also narrowed the overall band gap of the composite system to extend the light response range to the visible region, realizing efficient degradation of antibiotic residues. Furthermore, the membrane maintained negligible flux decline after multiple cyclic uses, confirming its excellent reusability. This work highlights the synergistic regulation of TiO2/ZnO on the structure, wettability, and mechanical stability of carbon nanofiber membranes, providing a scalable strategy for fabricating high-performance self-cleaning membranes in aquatic pollution remediation.

La-doped flexible hydrophobic Al2O3 fiber membranes: preparation, properties, and oil–water separation performance

Feng Changjian,Yang Hongkai,Huang Lilan,Lv Lin,Li Jiao

In this study, a La-doped flexible hydrophobic Al 2 O 3 fiber membrane was fabricated via electrospinning technology, and the influence of La doping on the mechanical properties and surface wettability of the Al 2 O 3 fiber membrane was systematically investigated. The results showed that the La-doped modified alumina nanofiber membrane has tunable fiber diameters (180–480 nm) and a highly interconnected porous network, which enhanced the efficiency and flux of oil–water separation. The incorporating an appropriate amount of rare earth La atoms, specifically at a La/Al atomic ratio of 0.125 (La-Al 2 O 3 -2), effectively enhanced the flexibility and tensile strength of the membrane. Concurrently, hydrophobic La 2 O 3 formed on the fiber surface, which transformed the membrane from hydrophilic to hydrophobic. As a result, the La-doped Al 2 O 3 membrane exhibited excellent anti-fouling performance, high permeation flux, and efficient oil–water separation. However, excessive La addition led to a significant decline in the flexibility and tensile strength of the Al 2 O 3 fiber membrane, whereas the hydrophobic surface was largely preserved. Among all samples, the La-Al 2 O 3 -2 fiber membrane exhibited superior flexibility and tensile strength. In oil–water separation applications, it achieved a high permeation flux of 2921 L/(m 2  ·h) and a separation efficiency of 98.4%; meanwhile, the good reusability was also obtained. Graphical abstract

Dip-coated TiO2-SSM with polarity-regulated underliquid superlyophobicity for high-flux separation of immiscible liquids

Jing Zhang,Jiale Li,Kun Zhang,Yingyi Li,Yanting Zhang,Nan Jiang,Peng Chen,Ming Zhang,Meitong Li

Efficient separation of immiscible organic liquids and oil/water mixtures remains a critical bottleneck in industrial wastewater management due to their similar polarities and surface energies. A TiO 2 -functionalised stainless steel mesh (TiO 2 -SSM) was fabricated through a scalable sol−gel dip-coating route, with TiO 2 (8.0)-SSM (8.0 vol% TBOT) as the optimal architecture. Pre-wetting with a high-polarity liquid generates a stable liquid-infused interface that can be tuned on demand, endowing the membrane with exceptional underliquid superlyophobicity toward low-polarity liquids. The membrane separates at least 23 different liquid mixture pairs, with efficiencies exceeding 99% for 20 of these pairs and a excellent flux of 34,200 L·m −2 ·h −1 for formamide/n-hexane. The membrane exhibits exceptional durability, with <2% efficiency decline after 50 cycles, intact performance after 30 abrasion tests, and robust resistance to acid, alkali, and saline environments. Notably, TiO 2 (8.0)-SSM combines robust mechanical/chemical stability with remarkable photocatalytic activity, achieving >98.3% degradation of MB solution under simulated solar irradiation. This polarity-switchable TiO 2 (8.0)-SSM enables simultaneous separation and degradation of complex wastewater containing both immiscible liquids and soluble pollutants, offering a cost-effective solution for industrial wastewater treatment and resource recovery.

Biomimetic “Flower-on-Fiber” Janus membrane with Z-scheme heterojunctions for synergistic oily wastewater remediation: Visible-light photocatalysis, unidirectional transport and dual-mode antibacterial activity

Zhengyu Ding,Yalong Liu,Jiali Yu,Zheyao Xia,Jun Zhang,Kainan Guo,Jinke Guo,Binjie Xin,Shouzhi Yan

Biomimetic “flower-on-fiber” Janus membranes were engineered. • Achieved >99% emulsion separation and 97.4% TCH degradation under visible light. • Proposed Z-scheme supported by EPR/DFT boosts visible-light charge separation. • Asymmetric wettability enables unidirectional oil transport and “Janus boat” application. • Synergistic physical/photocatalytic mechanism confers 99.99% antibacterial rate. The remediation of complex industrial wastewater—characterized by the coexistence of emulsified oils, soluble organic micropollutants, and pathogenic bacteria—necessitates a paradigm shift from passive filtration to active, multifunctional interface engineering. To address this, a novel biomimetic “flower-on-fiber” Janus membrane was engineered via a sequential electrospinning and spray-deposition strategy. The membrane features a rationally designed asymmetric architecture, integrating a hydrophobic Z -scheme photocatalytic top-layer (BiOBr/ZnO/TiO 2 @PVDF) with a hydrophilic substrate (PAN@PEG). Molecular Dynamics (MD) simulations were used as qualitative support to examine the water/BiOBr interfacial behavior, indicating that water molecules tend to remain aggregated rather than spread on the BiOBr lattice. Consequently, the membrane achieved high-efficiency separation (>99%) of surfactant-stabilized water-in-oil emulsions with a superior flux of 2120 ± 119 L m −2  h −1 . Beyond physical separation, the constructed heterojunction promoted visible-light absorption and suppressed charge recombination. This endowed the membrane with exceptional “active” defense capabilities: a 97.4% degradation efficiency for tetracycline hydrochloride (TCH) and a 99.99% antibacterial rate against E. coli via a synergistic “physical puncture-photocatalytic oxidation” dual-mode mechanism. This work demonstrates a proof-of-concept Janus membrane platform that couples structural biomimicry with Z -scheme heterojunction engineering to integrate water-in-oil emulsion separation, visible-light-driven tetracycline degradation, and antibacterial activity against E. coli .

Assessment of p-Xylene Migration Behaviour in Unsaturated Soil Systems: A 3-D Sand Flow Chamber Study

The influence of soil water content and contaminant injection pressure on the migration behaviour of p-Xylene (one of the organic pollutants in Benzene Toluene Ethylbenzene & Xylene) is investigated in the unsaturated zone using the controlled laboratory sandbox experiments. The results show that, at equivalent migration distances, higher soil water content leads to longer migration durations and reduces transport velocities, whereas greater injection pressure promotes faster contaminant movement. These findings highlight the governing role of water saturation in the water–oil–gas three-phase system, in which increased water content decreases the relative permeability of the oil phase and retards capillary-driven BTEX transport. Unlike many previous studies in which contaminants commonly migrate downward toward the groundwater table, the BTEX in this study did not reach the water table and exhibited partial upward migration. Overall, the results provide experimental-scale insight into key mechanisms governing BTEX transport in unsaturated sandy media and serve as a reference for improving predictions of contaminant fate and migration pathways in field settings.

Janus membrane with fluorinated graphene oxide embedded PVDF-HP hydrophobic layer for efficient oil/water separation

Yiqiao Xie,Hanyun Zhou,Yang Yu

The efficient and low-cost treatment of oily wastewaters has become a concerned issue for mitigating the environmental pollution and reducing resource wastage. In this study, a novel Janus membrane was fabricated by electrospinning a thin hydrophobic layer of fluorinated graphene oxide (FGO) nanosheet-incorporated poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) nanofibers on the surface of a polyacrylonitrile (PAN) hydrophilic layer. The hydrophobic layer was controlled to be 15 times thinner than that of the hydrophilic layer by regulating the electrospinning time. The incorporation of FGO nanosheets into PVDF-HFP nanofibers induced the formation of numerous microspheres and finer nanofibers on the hydrophobic layer, thereby endowing the membrane with a high surface roughness with a “beads-on-strings” morphology. The PVDF-HFP/FGO hydrophobic layer exhibited a water contact angle of 136.8°, which contributed to a high oil flux of ~17500 L/m2.h and separation efficiency of 95.3% for the heavy oil/water mixture. In contrast, the membrane achieved a water flux of ~5800 L/m2.h and separation efficiency of 99.1% for the light oil/water mixture. The membrane could maintain stable performance over seven consecutive separation cycles and within the temperature range of 20–120°C. The prepared membrane could be considered as a promising alternative for the practical treatment of oily wastewaters.

Bioinspired Janus Interpenetrating Dressing with Unidirectional Oil-Phase Biofluid Drainage for Enhanced Acne Healing

Dan Zhao,Jingchong Liu,Lanlan Hou,Li Yang,Guotao Liu,Changtao Wang,Xiaoshan Zhang,Yulu Huo,Yongqiang Wen

Acne vulgaris is a prevalent chronic inflammatory skin condition driven by excessive sebum secretion and follicular blockage. While most existing acne patches emphasize downstream effects, such as antibacterial and anti-inflammatory effects, they neglect sebum accumulation, a key upstream factor. Efficient removal of this lipid-rich biofluid remains a major challenge. Although existing unidirectional drainage systems have shown success in managing aqueous wound exudates through capillary-driven flow, their designs are poorly suited for viscous, nonpolar secretions like sebum. Here, we present a Janus interpenetrating dressing (BJID) consisting of an oleophilic absorbing layer/underwater oleophobic barrier layer to form a structurally interlocked interface. Mimicking natural liquid collection and directional self-transport mechanisms, the BJID achieves unidirectional oil-phase sebum drainage while preventing backflow. Taking advantage of conical micropores, anisotropic wettability, and directional capillary forces, BJID enables a high rectification ratio of 4.4 and directional transport of sebum droplet while inhibiting reverse diffusion. It also reduces intracellular ROS and suppresses inflammation via the TLR2/NF-κB pathway in C. acnes-stimulated HaCaT cells. In a three-week human trial, BJID reduced acne size and sebum by 31.8% and 21.3% and increased skin hydration by 13.1%. These findings highlight the potential of bioinspired unidirectional oil-phase drainage materials as a new strategy for regulating skin microenvironments and improving acne therapy.

Hydrophobic sandwich-like PVDF/rGO/PDMS composite membrane with fast electrothermal/photothermal response for crude oil removal

Jiale Zhou,Kunquan Li,Zhuohan Chen,Xiaojing Su,Huali Xie,Lei Yao,Yunhui Wu,Xiaofan Zhang,Lu Chen,Xuting Wu,Wenjian Wu

Functional membranes with electrothermal/photothermal effects are considered an effective method for separating water/viscous crude oil mixtures. However, the loading of photothermal and electrothermal particles in polymeric membranes with poor compatibility will deteriorate the membrane performance, leading to the unstable photothermal/electrothermal effects. Therefore, we prepared a hydrophobic sandwich structure polyvinylidene fluoride/reduced graphene oxide/polydimethylsiloxane (PVDF/rGO/PDMS) composite membrane using a two-step electrospinning process, which improved the binding force between the photothermal/electrothermal particles and the matrix with the composite membrane exhibiting super fast electric heating and photothermal effect. The temperature of the composite membrane surface rapidly reached to 162.5 °C within 30 s at 25 V. In addition, the addition of graphene (GO) improved the mechanical properties of the composite membrane. Meantime, the composite membrane possessed stable hydrophobicity and lipophilicity, which was beneficial for separating water/viscous crude oil mixtures. Owing to the controllable pore structure and special wetting behavior, the water-in-oil emulsion was successfully separated. Importantly, the separation rates of the water/crude oil mixtures under the electrothermal effect was 2340 kg·m −2 ·h −1 ·bar −1 . Thus, this study provides a new method for preparing hydrophobic sandwich-like PVDF/rGO/PDMS composite membrane with electrothermal/photothermal effects, which has significant potential for engineering applications in high-viscosity crude oil removal and water-in-oil emulsion separation.

Amyloid-mediated remineralization for tooth hypoplasia of cleidocranial dysplasia

Guo Xiaohe,Yang Xiaoxue,Liu Peisheng,Huang Xiaoyao,Gu Yang,Guo Hao,Xuan Kun,Liu Anqi

Introduction: Cleidocranial dysplasia (CCD) is an autosomal-dominant, heritable skeletal and dental disease, involving hypoplastic clavicles, defective ossification of the anterior fontanelle, dentin and enamel hypoplasia, and supernumerary teeth, which can seriously affect the oral and mental health of patients. Amyloid-like protein aggregation, which is established by lysozyme conjugated with polyethylene glycol (Lyso-PEG), forms a mineralized nanofilm layer on a healthy enamel surface. However, whether it can form a remineralization layer in dental tissues from CCD remains unclear.Methods: This study evaluated deciduous teeth from healthy individuals and a patient with CCD. Because pulp and dentin are functionally closely related, stem cells from human exfoliated deciduous teeth (SHED) from CCD patients and healthy individuals were collected to compare their biological properties.Results: The results found that deciduous teeth from patients with CCD exhibited dentin hypoplasia. In addition, the proliferative ability and osteogenic potential of SHED from patients with CCD were lower than those of control individuals. Finally, Lyso-PEG was applied to dentin from the CCD and control groups, showing a similar remineralization-induced effect on the dentin surfaces of the two groups.Conclusion: These results extend our understanding of the dentin and SHED of patients with CCD, exhibiting good caries-preventive capacity and good biocompatibility of Lyso-PEG, thus providing a novel dental therapy for CCD and patients with tooth hypoplasia.