SCIENCE OF THE TOTAL ENVIRONMENT
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2023
·
IF 9.8
Kai Yang,Mo-Lian Chen,Dong Zhu
Disinfectants are routinely used in human environments to control and prevent the transmission of microbial disease, and this is particularly true during the current COVID-19 crisis. However, it remains unclear whether the increased disinfectant loadings to wastewater treatment plants facilitate the dissemination of antibiotic resistance genes (ARGs) in sewage sludge microbiomes. Here, we investigated the impacts of benzalkonium chlorides (BACs), widely used disinfectants, on ARGs profiles and microbial community structures in sewage sludge by using high-throughput quantitative PCR and Illumina sequencing. A total of 147 unique ARGs and 39 mobile genetic elements (MGEs) were detected in all sewage sludge samples. Our results show that exposure to BACs disinfectants at environmentally relevant concentrations significantly promotes both the diversity and absolute abundance of ARGs in sludge microbiomes, indicating the co-selection of ARGs by BACs disinfectants. The enrichment of ARGs abundance varied from 2.15-fold to 3.63-fold compared to controls. In addition, BACs exposure significantly alters bacterial and protistan communities, resulting in dysbiosis of the sludge microbiota. The Mantel test and Procrustes analysis confirm that bacterial communities are significantly correlated with ARGs profiles under BACs treatments. The structural equation model explains 83.8 % of the total ARGs variation and further illustrates that the absolute abundance of MGEs exerts greater impacts on the variation of absolute abundance of ARGs than microbial communities under BACs exposure, suggesting BACs may promote antibiotic resistance by enhancing the horizontal gene transfer of ARGs across sludge microbiomes. Collectively, our results provide new insights into the proliferation of antibiotic resistance through disinfectant usage during the pandemic and highlight the necessity to minimize the environmental release of disinfectants into the non-target environment for combating antibiotic resistance.
研究领域:
环境科学
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微生物学
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公共卫生
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2024
·
IF 8.2
Jinling Ma,Xiaojun Niu,Dongqing Zhang,Gang Wang
The chemical components in the natural aquatic environment have the potential to be involved in phototransformation of microplastics (MPs). Little information is available regarding the mediation effects of artificially introduced chemicals on MP phototransformation, especially those used in aquaculture water that are vulnerable to human interference. Herein, this study investigated the phototransformation process and mechanism of polypropylene microplastic (PP MPs) in presence of trichloroisocyanuric acid (TCCA) disinfectant with unique properties unlike the conventional inorganic chlorine disinfectants. The results showed that the presence of TCCA inhibited the surface photooxidation of PP MPs. Analysis of PP MP surface and reaction filtrate indicated that the inhibitory effects were likely derived from TCCA derivatives and the weakening in promoting effect of polypropylene microplastic-derived dissolved organic matter (PP-DOM) as photolytic byproducts, with the more important role of free chlorine in initial period and that of other chlorine species (i.e., the adsorbed chloride ions (Cl − ), newly formed carbon-chlorine (C Cl) bonds, chlorinated cyanurates, and chlorinated products) in middle and later period. The study highlights for the first time the important role of chlorine species derived from TCCA in phototransformation process of co-existed PP MPs and proposes a previously unrecognized phototransformation pathway, which will provide a new understanding and knowledge for the environmental behavior of MPs in aquaculture environment.
研究领域:
环境科学
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水化学
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材料科学
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环境工程
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PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yanli Zhou,Congming Li,Yuanqiang Hao,Baoxian Ye,Maotian Xu
High-density and cross-linked copper-based metal-organic framework (Cu-MOF) sheets were successfully prepared via a simple oriented growth method on a carboxylated graphene-modified electrode surface. Hydrogen peroxide (H 2 O 2 ) was selected as a model molecule to examine the performance of the thin film of Cu-MOF/graphene. The proposed sensor showed an extended linear detection range from 2.00 × 10 −7 to 1.85 × 10 −4 mol L −1 ( R = 0.998), a high sensitivity of 0.792 A (mol L −1 ) −1 , and a low detection limit of 6.7 × 10 −8 mol L −1 , due to the synergistic catalysis from the porous structure and favorable electron transfer mediating function of the electroactive Cu-MOFs and the high conductive property of the graphene. The reduction peak current of H 2 O 2 changed less than 3.7% in the presence of 57-fold high concentrations (2.0 × 10 −4 mol L −1 ) of the potential interfering species. The good selectivity of the prepared modified electrode was acquired by the size exclusion (molecular sieving) for H 2 O 2 because of the proper pore shape and pore size of Cu-MOFs. The feasibility of the assay was verified by test of H 2 O 2 in disinfectant samples. The proposed strategy presents valuable information related to the construction of non-enzymatic electrochemical sensors .
研究领域:
材料科学
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电化学
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分析化学
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传感器
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2024
·
IF 12.2
Jie-Yi Zeng,Wei Li,Jian-Qiang Su,Yan-Zi Wang,Yaying Li,Huaiying Yao
Disinfectants and antibiotics are widely used for the prevention and control of bacterial infectious diseases. Frequent disinfection is thought to exacerbate antibiotic resistance. However, little is known about how disinfectants and antibiotics co-induce changes in the soil antibiotic resistance genes (ARGs). This study determined the ARG profiles and bacterial community dynamics between unamended soil and manure-amended soil exposed to benzalkonium chloride (C 12 ) (BC, 10 mg kg −1 ) disinfectant and sulfamethazine (SMZ, 1 mg kg −1 ), using high-throughput quantitative PCR and 16 S rRNA gene sequencing. Manure application enriched the soil in terms of ARGs abundance and diversity, which synergistically amplified the co-selection effect of BC and SMZ on soil antibiotic resistome. Compared with the control treatment, BC and SMZ exposure had a smaller impact on the bacterial infectious diseases and antimicrobial resistance-related functions in manure-amended soil, in which bacterial communities with greater tolerance to antimicrobial substances were constructed. Manure application increased the proportion of rank I ARGs and potential human pathogenic bacteria, while BC and SMZ exposure increased the drug-resistant pathogens transmission risk. This study validated that BC and SMZ aggravated the antimicrobial resistance under manure application, providing a reference for managing the spread risk of antimicrobial resistance in agricultural activities.
研究领域:
环境科学
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微生物学
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土壤学
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
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2024
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IF 4.1
Fei Shi,Lixin Ma,Zhilong Chen,Yao Huang,Li Lin,Zhendong Qin
The gut microbiome is crucial in maintaining fish health homeostasis. Disinfectants can kill important pathogens and disinfect fish eggs, yet their effect on the immune pathways and intestinal microbiome in healthy fish remains unknown. In this study, we investigated the effects of two disinfectants on the transcriptome profiles, immunological response, and gut microbiota dynamics of grass carp over a four-week trial. In particular, aquatic water was disinfected with 80 μg/L glutaraldehyde or 50 μg/L povidone-iodine. We found that glutaraldehyde and povidone-iodine induced gut antioxidant system and depressed the function of grass carp digestive enzymes. The results of the 16S rDNA high-throughput sequencing identified a reduction in the diversity of grass carp gut microbiota following the disinfectant treatment. Moreover, transcriptome profiling revealed that disinfectant exposure altered the immune-related pathways of grass carp and inhibited the expression of inflammation and tight junction related genes. Finally, the histopathological observation and apoptosis detection results suggested that the long-term diet of disinfectant destroyed intestinal structural integrity and promoted apoptosis. In conclusion, long-term exposure to disinfectants was observed to reduce oxidation resistance, suppress the immune response, dysbiosis of the intestinal flora, and resulted in increasing the apoptosis in intestinal of grass carp.
研究领域:
水产养殖
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免疫学
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微生物学
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分子生物学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Wei Sun,Zi-Xi Wang,Yuxin Guo,Chengcheng Li,Ge Gao,Fu-Gen Wu
Iodine (I 2 ) as a broad-spectrum antiseptic has been widely used for treating bacterial infections. However, I 2 has low water-solubility and sublimes under ambient conditions, which limits its practical antibacterial applications. The highly specific and sensitive reaction between I 2 and starch discovered 200 years ago has been extensively applied in analytical chemistry, but the antibacterial activity of the I 2 − starch complex is rarely investigated. Herein, we develop a novel type of iodine-based antiseptics, iodine−soluble starch (I 2 -SS) cryogel, which can dissolve in water instantly and almost completely kill bacteria in 10 min at 2 μg/mL of I 2 . Although KI 3 and the commercially available povidone‑iodine (I 2 -PVP) solutions show similar antibacterial efficacy, the high affinity of I 2 to SS largely enhances the shelf stability of the I 2 -SS solution with ~75 % I 2 left after one-week storage at room temperature. In sharp contrast, ~8 % and ~ 3 % I 2 are detected in KI 3 and I 2 -PVP solutions, respectively. Mechanistic study reveals that the potent antibacterial effect of I 2 -SS originates from its attack on multiple bacterial targets. The outstanding antibacterial activity, capability of accelerating wound healing, and good biocompatibility of I 2 -SS are verified through further in vivo experiments. This work may promote the development of next-generation iodine-based antiseptics for clinical use.
研究领域:
药学
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材料科学
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化学
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微生物学
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生物医学工程
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PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2019
·Vol. 126
·期 1
·102-112
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IF 2.683
Lin W.,Yuan D.,Deng Z.,Niu B.,Chen Q.
ObjectivesIn our previous research, we have developed a new combination disinfectant, glutaraldehyde‐didecyldimethylammonium bromide (GD). It was verified that GD had a strong effect on both Escherichia coli and Staphylococcus aureus. In this work, Candida albicans was selected as an object, and it could be killed by GD. We aimed to investigate the cellular and molecular mechanism of GD effecting on C. albicans.Methods and ResultsThe results of sterilization experiment indicated that GD was effective on C. albicans. Flow cytometry and atomic absorption spectrometry were applied to detect cell membrane damage of C. albicans. Luciferase reaction and Bradford method were carried out to detect ATP content and protein quantitation. Transmission electron microscopy was used for intracellular organelles morphological observation. In order to study changes in mitochondrial membrane potential, Rh 123 was used as an indicator. DNA conformation analysis was performed by molecular modelling and circular dichroism. The results indicated that membrane permeability was increased rapidly owing to GD effect, and the leaked K+ and Mg2+ were about 12·1 and 12·4 times those of the control, respectively, at 10 min after GD treatment. Simultaneously, ATP and protein also leaked rapidly out of the cell. Mitochondrial membrane potential was destroyed, succinic dehydrogenase activity was significantly decreased and DNA conformation was changed because of GD action.ConclusionsGlutaraldehyde‐didecyldimethylammonium bromide disinfected C. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K+, Mg2+, ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death.Significance and Impact of the StudyThis study focused on the cellular and molecular mechanism of GD as a disinfectant against C. albicans. It is important to provide theoretical support to GD against Candida albicans in practical application.
研究领域:
微生物学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2023
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IF 6.7
Chengcheng Qi,Yuhua Luo,Yongping Dong
The study of single atoms in the field of electrochemical sensing is at the early stage. How to boost the catalytic performance of single atoms is still a big challenge. Herein, we prepare Fe-Se dual single-atom sites on N-doped ultrathin carbon carrier (Fe 1 Se 1 /NC) for realizing highly sensitive nonenzymatic detection of H 2 O 2 . Fe 1 Se 1 /NC was synthesized through the pyrolysis strategy, and then was characterized by transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS) mapping, high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), X-ray diffraction (XRD), X-ray photoelectron spectrometer (XPS), and electrochemical techniques, respectively. The results displayed that Fe and Se both existed in an atomically dispersed status. Compared to the single-atom Se or Fe catalyst (Se 1 /NC or Fe 1 /NC), the Fe 1 Se 1 /NC significantly boosted the electrocatalytic activity for H 2 O 2 reduction. The Fe 1 Se 1 /NC modified electrode could be used to detect H 2 O 2 in a wide linear range of 0.02 mM to 13 mM with a high sensitivity of 1508.6 µA·mM −1 ·cm −2 and a low detection limit of 11.5 µM. Moreover, the sensor was successfully employed for detecting H 2 O 2 in disinfectant and urine samples. This work provides a novel design of dual-atom catalysts for excellent electrochemical sensing applications.
研究领域:
电化学
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催化
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材料科学
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分析化学
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DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
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2018
·Vol. 124
·期 5
·1060-1070
·
IF 2.16
Lin W.,Yi J.,Zhang Y.,Deng Z.,Chen Q.,Niu B.
AimsThe effects of a new glutaraldehyde–didecyldimethylammonium bromide combination disinfectant (GD) on the cell surface of Staphylococcus aureus, a representative Gram‐positive bacterium, were investigated in this study.Methods and ResultsResults of bacterial surface structural analysis showed that GD significantly changed the bacterial morphology. The membrane fluidity decreased and outer membrane permeabilization increased after contact with GD. Furthermore, the integrity of the cytoplasmic membrane was destroyed by over 99% after exposure to GD for a short time. Bacterial ATPase activity correlated negatively with the treatment of GD over time, and proteins were degraded. Assays of intracellular component leakage indicated that GD caused the rapid leakage of K+, Mg2+, ATP molecules, and proteins into the extracellular environment.ConclusionsThe effects of GD against S. aureus are probably attributable to the removal of the permeability barrier, changes in the S. aureus morphology, changes in the structures and functions of the cell membrane, leakage of intracellular substances and disturbance of the intracellular homeostasis. As a result, this irreversible damage accelerated the death of S. aureus.Significance and Impact of the StudyIn an earlier study, the bactericidal mechanism of GD against Escherichia coli was investigated. Hence, this study focused on the action of mechanism of GD against S. aureus. It is important to clarify the disinfectant bactericidal mechanisms of GD against bacterium, in general, and this study provides theoretical support to the prevention of bacterial resistance.
研究领域:
微生物学
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药理学
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传染病学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2021
·
IF 5.277
Qing Chen,Yaxin Liu,Ziyi Zhang,Ke Li,Bin Liu,Tianli Yue
Glycerol monolaurate (GML) is a monoglycerol ester of the fatty lauric acids, which has a wide-spectrum antimicrobial capacity, but fails to inactivate Gram-negative bacteria, especial Salmonella . To enhance the population reduction rate of GML for Salmonella , this reagent was combined with three disinfectants: lactic acid (LA), cetylpyridinium chloride (CPC), and trisodium phosphate (TSP), which can present acid, neutral, and alkaline in solution, respectively. The results showed that the 1% GML and a complex disinfectant (0.5% GML-0.025% LA) could powerfully inactivate Salmonella . Their population reduction rates respectively were able to achieve 99.92% and 98.29% with the vortex treatment, indicating that the vortex treatment could improve GML to destruct the outer membrane of Salmonella . During the simulation test of the soaking and rinse processing of chicken, for a short time (0 h), the effect of 0.5% GML-0.025% LA compound was better and more suitable for instantaneous inactivation than others, while for a long time (4 h), 1% GML exhibited a better bactericidal effect, which indicated it to be more suitable for long-term bacteriostasis. The characterization of color and texture for chicken samples were determined using Colormeter Ci7600, TA.XT Plus and Hyper-spectral Imager, which demonstrated that all samples treated by these complex disinfectants were not significantly different from untreated group. In conclusion, GML is a potential and superior disinfectant for the chicken process.
研究领域:
食品科学
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微生物学
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食品安全
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2012
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IF 4.525
Xiaojuan Liu,Chenghua Zong,Kelong Ai,Wenhui He,Lehui Lu
Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical tool. However, its applications for in situ detection of target molecules presented on diverse material surfaces have been hindered by difficulties in rapid fabricating SERS-active substrates on the surfaces of these materials through a simple, low-cost, and portable approach. Here, we demonstrate our attempt to address this issue by developing a facile and versatile method capable of in situ generating silver nanoparticle film (SNF) on the surfaces of both artificial and natural materials in a simple, cheap, practical, and disposable manner. Taking advantage of the high SERS enhancement ability of the prepared SNF, the proposed strategy can be used for in situ inspecting herbicide and pesticide residues on vegetables, as well as the abuse of antiseptic in aquaculture industry. Therefore, it opens new avenues for advancing the application prospects of SERS technique in the fields of food safety, drug security, as well as environment monitoring.
研究领域:
分析化学
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材料科学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Mengying Xu,Pier-Luc Tremblay,Muhammad Babur Joya,Abanchime Zenaba Hadja Kaka,Emmanuel Seneway Kollah,Blessings Kapungwe Mwansa,Wenhao Wang,Yang Liu,Xiaolin Xing,Feijun Qiu,Tian Zhang
Humic acids (HAs) in water react with chlorine disinfectants and form carcinogenic chemicals. These molecules also inhibit environmental engineering processes aiming at the removal of toxic metals and organic contaminants from wastewater. For these reasons, low-cost adsorption strategies are being developed to take out HAs from water. Here, the metal oxide Bi 2 MoO 6 with either exposed [001] (BMO-001-Br) or [010] (BMO-010-Br) facets prepared with the cetyltrimethylammonium bromide (CTAB) surfactant was evaluated for the adsorption of HAs. With a maximal adsorption capacity for HAs of 63.1 mg/g, BMO-001-Br nanosheets constituted a superior adsorbent capable of removing contaminants over a large pH range. BMO-001-Br was reusable and could separate HAs from multiple solutions including tap water, synthetic wastewater, and real pharmaceutical wastewater. The adsorption capacity of BMO-001-Br can be explained by its suitable specific surface area and its positive surface charge related to the presence of CTAB molecules attracting negatively charged HA molecules. A third feature of BMO-001-Br involved in HAs removal is a high abundance of oxygen vacancies, which are easier to form on a BMO surface with exposed crystal [001] facets. The notable performance of BMO-001-Br indicates that it can serve efficiently for the extraction of HAs from different aqueous solutions.
研究领域:
环境工程
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水处理
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）