FOOD CHEMISTRY
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2026
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IF 10.4
Mengli Han,Xin Jiang,Meijiao Gui,Qianqian Liang,Yuan Gu,Xin Song,Wenzheng Shi
In this study, we investigated the effects of four phosphates on the structure of myofibrillar proteins and the gel properties of surimi. The results indicate that phosphate-modified proteins exhibit lower particle size, zeta potential, and surface hydrophobicity. Among them, sodium pyrophosphate (TSPP) and sodium tripolyphosphate (STPP) could depolymerize proteins, sodium hexametaphosphate could repolymerize proteins, and sodium trimetaphosphate enables proteins to partially reaggregate after dissociation. The addition of TSPP and STPP to squid surimi gel enhances hydrophobic interactions, improving protein cross-linking, thereby reducing the pore space of squid surimi gel and increasing gel strength, texture profile analysis (TPA) properties, and water holding capacity (WHC). This provides a reference for the use of phosphates during the processing of surimi products.
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ADVANCED MATERIALS
·
2022
·Vol. 35
·期 9
·2209777
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IF 32.086
Hongfeng Li,Haohao Shi,Xinyi Chen,Zhen Ren,Yu Shen,Peng Wu,Yun Fan,Xinglong Zhang,Wenxiong Shi,Honggang Liao,Suoying Zhang,Weina Zhang,Fengwei Huo
The construction of metal–organic framework (MOF) films is a crucial step for integrating them into technical applications. However, due to the crystallization nature, it is difficult to grow most MOFs spontaneously or process them into films. Here, a convenient strategy is demonstrated for constructing MOF films by using modulators to achieve homogeneous assembly of MOF clusters. Small clusters in the early growth steps of MOFs can be stabilized by modulators to form fluidic precursors with good processibility. Then, simple removal of modulators will trigger the crosslinking of MOF clusters and lead to the formation of continuous films. This strategy is universal for the fabrication of several types of MOF films with large scale and controllable thickness, which can be deposited on a variety of substrates as well as can be patterned in micro/nano resolution. Additionally, versatile composite MOF films can be easily synthesized by introducing functional materials during the crosslinking process, which brings them broader application prospects.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Shixin Song,Yufei Li,Qiyuan Zhu,Xin Zhang,Yang Wang,Li Tao,Lei Yu
In this paper, buckwheat protein colloidal particles (BPCPs) were prepared by heat treatment to stabilize oil-water interface. The results of particle size, surface hydrophobicity and wettability indicated that the prepared BPCPs could be used as novel Pickering emulsifier. The effects of BPCPs concentration, ionic strength and heat treatment on the structure and properties of Pickering emulsions were explored. The microstructure results showed that BPCPs could tightly coated on the surface of oil droplets to form a tight interfacial film, confirming that BPCPs could be used as an effective Pickering-like stabilizer. With the increase of BPCPs concentration, the droplet size of the Pickering emulsion gradually decreased, and the viscoelasticity and storage stability of the emulsion were effectively improved. Different from the effect of ionic strength, heat treatment was beneficial to increasing the viscoelasticity of BPCPs-stabilized Pickering emulsion. The Pickering emulsions exhibited certain flocculation at different temperatures and ionic strengths, while still maintained good solid-like behavior. These results suggest that the structure and properties of BPCPs-stabilized Pickering emulsion could be regulated by changing the ionic strength and temperature.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Xiqing Liu,Tao Wang,Yongqing Wang
In this study, a conversion method and molecular imprinting technology were used to design molecularly imprinted polymers (MIP)-based ratiometric fluorescence test papers. The ZnO quantum dots (ZnO QDs) acted as the background quantum dots and ZIF-8 raw material. Carbon dots (CDs) were used as the identification signals. The imprinting layer achieved a selective function. Therefore, a ZnO@ZIF-8/CDs@MIPs sensor was designed for the detection of Bisphenol A (BPA). The sensor exhibited a fast response time for BPA detection. In addition, the sensor demonstrated that effective detection of BPA can still be achieved in complex environments. The detection limit of this sensor was 0.778 nM with a linear range of 0–60 nM. The corresponding test solutions exhibited clear changes from blue to yellow. The selectivity experiments results demonstrated that ZnO@ZIF-8/CDs@MIPs only exhibit excellent selective recognition effect for BPA. ZnO@ZIF-8/CDs@MIPs-2 was used for the detection of BPA in canned food and compared with the results of HPLC detection of BPA. The two spiked recovery ranges were 96.58–102.04% and 97.43–103.82%, respectively. In addition, the prepared ZnO@ZIF-8/CDs@MIPs-2 test paper visually recognized BPA under ultraviolet light. This study provides guidelines for the design and application of fluorescent test papers for quick detection in practical applications.
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Cell Reports
·
2023
·
IF 8.8
Ting Zeng,Jie Yin,Ziwen Liu,Zhaoxing Li,Yu Zhang,Yang Lv,Mei-Ling Lu,Min Luo,Meirong Chen,Yibei Xiao
Summary The type V-K CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration and have great potential as a programmable site-specific gene insertion tool. Although all core components have been independently characterized structurally, the mechanism of how the transposase TnsB associates with AAA+ ATPase TnsC and catalyzes donor DNA cleavage and integration remains ambiguous. In this study, we demonstrate that TniQ-dCas9 fusion can direct site-specific transposition by TnsB/TnsC in ShCAST. TnsB is a 3′-5′ exonuclease that specifically cleaves donor DNA at the end of the terminal repeats and integrates the left end prior to the right end. The nucleotide preference and the cleavage site of TnsB are markedly different from those of the well-documented MuA. We also find that TnsB/TnsC association is enhanced in a half-integration state. Overall, our results provide valuable insights into the mechanism and application expansion of CRISPR-mediated site-specific transposition by TnsB/TnsC.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Xue Liu,Li Wang,Bowen He,Qiao Liu,He Zhu,Andrew J. Carrier,Ken D. Oakes,Xu Zhang
Gallic acid (GA, 3,4,5-trihydroxybenzoic acid) is a widely used natural food additive of interest to food chemistry researchers, especially regarding its effects on myofibrillar protein (MP) oxidation. However, existing studies regarding MP oxidation by GA-combined with Fenton reagents are inconsistent, and the detailed mechanisms have not been fully elucidated. This work validated hydroxyl radical (HO·) as the primary oxidant for MP carbonylation; in addition, it revealed three functions of GA in the Fenton oxidation of MP. By coordination with Fe(III), GA reduces Fe(III) to generate Fe(II), which is the critical reagent for HO· generation; meanwhile, the coordination improves the availability and reactivity of Fe(III) under weakly acidic and near-neutral pH, i.e., pH 4–6. Second, the intermediates formed during GA oxidation, including semiquinone and quinone, promoted Fenton reactivity by accelerating Fe catalytic cycling. Finally, GA can scavenge HO· radicals, thus exhibiting a certain degree of antioxidant property. All three functions contribute to MP oxidation as observed in GA-containing meat.
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Petroleum Science
·
2023
·
IF 5.6
Xiang-Yu Wang,Ming-Wei Zhao,Xu-Hao Wang,Peng Liu,Meng-Yao Fan,Teng Li,Zhen-Feng Ma,Ying-Jie Dai,Cai-Li Dai
Fracturing fluid property play a critical role in developing unconventional reservoirs. Deep eutectic solvents (DESs) show fascinating potential for property improvement of clean fracturing fluids (CFFs) due to their low-price, low-toxicity, chemical stability and flexible designability. In this work, DESs were synthesized by mixing hydrogen bond acceptors (HBAs) and a given hydrogen bond donor (HBD) to explore their underlying influence on CFF properties based on the intermolecular interactions. The hydrogen-bonding, van der Waals and electrostatic interactions between DES components and surfactants improved the CFF properties by promoting the arrangement of surfactants at interface and enhancing the micelle network strength. The HBD enhanced the resistance of CFF for Ca 2+ due to coordination-bonding interaction. The DESs composed of choline chloride (ChCl) and malonic acid show great enhancement for surface, rheology, temperature resistance, salt tolerance, drag reduction, and gel-breaking performance of CFFs. The DESs also improved the gel-breaking CFF-oil interactions, increasing the imbibition efficiencies to 44.2% in 74 h. Adjusting HBAs can effectively strengthen the intermolecular interactions (e.g., HBA-surfactant and HBD-surfactant interactions) to improve CFF properties. The DESs developed in this study provide a novel strategy to intensify CFF properties.
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Qingqing Yu,Tong Shi,Zhiyu Xiong,Li Yuan,Hui Hong,Ruichang Gao,Yulong Bao
In order to study the effect of oxidation on the dye-binding behavior of myofibrillar proteins, selected dyes with different charges (positively charged Sarfarin O (SO), neutral bromophenol blue (BPB), and negatively charged Orange G (OG)) were incubated with myofibrils oxidized by the Fenton system with H 2 O 2 (10 mM). Upon oxidation, loss of free thiols, formation of carbonyls, particle size, and hydrophobicity of myofibrillar proteins (MPs) increased. The absolute value of Zeta-potential increased by 14.48 % after oxidation, the myofibrillar proteins shifted to a more acidic isoelectric point (pI) upon oxidation. Oxidation decreased net positive charges of myofibrillar protein and the binding ability of MPs towards OG in the environment with pH less than pI and the affinity of MPs towards SO in the environment with pH more than pI were thus increased. Here we propose a hypothesis that oxidation-induced change in net charges is the driving force affecting the amount of protein-bound dye. This paper aims to examine the effect of oxidation on the net charges of myofibrillar proteins and to provide insight into the mechanism of oxidation-induced changes in protein-bound dyes.
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Food Chemistry-X
·
2022
·
IF 6.443
Xin Luo,Jiankang Lu,Yongyan Wu,Wenshan Duan,Fengping An,Qun Huang,Lei Chen,Shaofeng Wei
Potential allergenicity of amandin was reduced by binding amandin with (−)-epigallocatechin gallate (EGCG) via alkaline, free radical, ultrasound-assisted alkaline, and ultrasound-assisted free radical methods. These results of total phenol content, free sulfhydryl group, free amino group, surface hydrophobicity, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) indicated that amandin might be covalently bound to EGCG through reactive groups such as sulfhydryl and amino groups, or non-covalently through hydrophobic interactions. Fourier transformed infrared (FT-IR) spectroscopy and fluorescence spectroscopy revealed structural changes of amandin-EGCG conjugate, which also caused significant reduction in potential allergenicity of amandin. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) found that amandin bound to EGCG mainly through cysteine and glutamate residues, and linear epitope for amandin was reduced. This provided a new method and theoretical basis of hypoallergenic almond food.
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Nanomaterials
·
2022
·Vol. 12
·期 20
·3636
·
IF 5.719
Jianchao Gong,Tingting Wang,Wei Zhang,Lin Han,Mingxiao Gao,Tianen Chen,Tao Shen,Yaxiong Ji
To explore the effect of spacer structure on the adsorption capability of organo-vermiculites (organo-Vts), a series of aza-containing gemini surfactants (5N, 7N and 8N) are applied to modify Na-vermiculite (Na-Vt). Large interlayer spacing, strong binding strength and high modifier availability are observed in organo-Vts, which endow them with superiority for the adsorption of 2-naphthol (2-NP) and bromophenol blue (BPB). The maximum adsorption capacities of 5N-Vt, 7N-Vt and 8N-Vt toward 2-NP/BPB are 142.08/364.49, 156.61/372.65 and 146.50/287.90 mg/g, respectively, with the adsorption processes well fit by the PSO model and Freundlich isotherm. The quicker adsorption equilibrium of 2-NP than BPB is due to the easier diffusion of smaller 2-NP molecules into the interlayer space of organo-Vts. Moreover, stable regeneration of 7N-Vt is verified, with feasibility in the binary-component system that is demonstrated. A combination of theoretical simulation and characterization is conducted to reveal the adsorption mechanism; the adsorption processes are mainly through partition processes, electrostatic interaction and functional interactions, in which the spacer structure affects the interlayer environment and adsorptive site distribution, whereas the adsorbate structure plays a role in the diffusion process and secondary intermolecular interactions. The results of this study demonstrate the versatile applicability of aza-based organo-Vts targeted at the removal of phenols and dyes as well as provide theoretical guidance for the structural optimization and mechanistic exploration of organo-Vt adsorbents.Keywords:organo-vermiculite;aza-containing gemini surfactant;2-Naphthol;bromophenol blue;adsorption
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JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Hui Liu,Mengya Li,Keyong Tang,Jie Liu,Xiumin Li,Xueping Meng
Conformations of secondary and super secondary structure from bovine hides collagen are very important for constructing collagen molecular space structure, and directly related to its properties. In this work, the evolutional mechanism of the collagen conformation in the environment of sodium sulphide (Na 2 S) solution is periodically studied by FT-IR spectroscopy, the Gaussian peak fitting of secondary derivative, and auxiliary methods such as SDS-PAGE, SEM and TG. As a result, the three polypeptide chains structure of bovine hide collagen was hardly affected by the ions of OH − , HS − and S 2− . The conformation of collagen molecules such as β-turn, triple helix and β-strand were gradually evolved into unordered structure and α-helix with the sequential addition of OH − , HS − and S 2− , which is because the hydrogen bond inside collagen molecular is extended or opened with the increase of the synergistic effect of pH and reducibility of HS − and S 2− in the solution. The bound water content and thermal properties of the treated collagen molecules are decreased. Finally, the order of the hydrolysis ability to collagen is NaOH < NaHS < Na 2 S at the same condition, which was confirmed by measuring the content of hydroxyproline from their solutions after treating the collagen. This work provides very important information for understanding the evolutional mechanism of bovine hide collagen conformation in the Na 2 S solution, developing the alternatives of sulfide, extraction of collagen, tanning, and application of collagen in biological and material science.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Hong Zeng,Hongli Yang,Yue Tang,Xiaojuan Niu,Yuangen Wu
A facile and simple colorimetric biosensor was first established for paraquat (PQ) detection based on the aptamer-enhanced oxidation process of 3,3′,5,5′-tetramethylbenzidine (TMB) by Ag + . The study confirmed that the interaction of PQ-15 aptamer with Ag + accelerates the electron transfer from the aptamer-Ag + complex to dissolved oxygen, which enhances the release of superoxide anion radicals (O 2 ̇ − ) and facilitates the catalytic oxidation of the chromogenic substrate. PQ-15 aptamer will preferentially bind to PQ molecules, resulting in no further enhancement of the catalytic activity of Ag + . Molecular docking results revealed that the PQ molecules are attached to the stem-loop region of the PQ-15 aptamer through σ-π conjugation interactions. The proposed method is simple that only contains Ag + and corresponding aptamer. The limit of detection (LOD) of the constructed colorimetric biosensor for PQ detection was determined to be 16.5 μg·L -1 , belowing the maximum residue limit in fruits and vegetables set by the EU. Moreover, the colorimetric biosensor showed excellent selectivity and anti-interference properties, which was validated for detecting PQ residues in several typical agricultural and water samples.
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