ENERGY & FUELS
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2022
·
IF 4.654
Zhijun Zhao,Jubao Gao,Mingsheng Luo,Xinyue Liu,Yongsheng Zhao,Weiyang Fei
In recent decades, ionic liquids (ILs) have been developed as an ideal and reversible decarbonization solvent. However, some pitfalls, such as the low CO2 capacity and high viscosity of ILs, limit their further scale up and industrial application. Therefore, in this work, three novel functional ILs [N8881][NIA], [N8881][For], and [N8881][Ac] were synthesized after using a molecular design method to capture CO2 at 303.15–333.15 K and pressures up to 1000 kPa. The experimental results illustrate that [N8881][NIA] presents the smallest viscosity and the highest CO2 solubility. The capacity order is [N8881][NIA] > [N8881][For] > [N8881][Ac] under the same experimental conditions. The CO2 recyclability experiments using [N8881][NIA] show the stability of CO2 solubility after 5 cycles. The quantum chemistry simulations at the level of DFT/B3LYP with 6-311++G(d,p) basis sets were used to study the CO2 absorption mechanism in the studied ILs from the molecular viewpoint. Simulation results illustrated that the higher interaction energy between CO2 and ILs means more CO2 absorbed in these ILs, which agreed well with the experimental results. The atoms in molecules theory and the function of the reduced density gradient were also used to calculate the interactions in these IL–CO2 systems. Results show that the majority of these interactions present an electrostatic character.
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BIOCHEMICAL ENGINEERING JOURNAL
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2022
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IF 4.446
Shan Yuan,Miaomiao Jin,Chao Xu,Wencheng Yin,Mingsha Zhao,Mo Xian,Wei Liu
3-(Aminomethyl) pyridine (3-AMP) is a versatile compound for the synthesis of pharmaceuticals, agricultural chemicals and numerous polymers. Efficient and mild biocatalytic synthesis is often an excellent supplement to traditional chemical synthesis methods. However, there has been no report of biocatalytic 3-AMP synthesis. In this paper, 3-AMP was synthesized for the first time by using biocatalysts co-expressing carboxylic acid reductase from Neurospora crassa (ncCAR) and ω-transaminase from Ochrobactrum anthropi (OATA) in one pot. In addition, the metabolic pathway was optimized by knocking out the transcriptional dual regulator arcA , which resulted in a 90% increase in the yield of 3-AMP. Furthermore, efficient self-sufficient whole-cell bioconversion was established by optimization, achieving 93% yield and a 40.6 mM (4.4 g/L) titer of 3-AMP without additional carbon sources. This whole-cell bioconversion system was also suitable for the high-efficiency biocatalytic synthesis of other aromatic or heterocyclic primary amines, such as benzylamine, 2-furfurylamine, 4-fluorobenzylamine, 2,4-difluorobenzylamine and 2-(aminomethyl) pyridine. Being entirely biocatalytic, our one-pot procedure provides considerable advantages in terms of economic, environmental and safety impacts over reported chemical methods.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Haizhi Zhang,Hongyuan Wu,Xinguang Qin,Ying Shen,Xinlin Wei,Gang Liu
Metalloporphyrin and gold nanoparticles (AuNPs) were fixed on the surface of hollow zeolite imidazole framework-8 (HZIF-8) by cation exchange and cross-linking reaction. The obtained hybrid nanozyme, Au/ [email protected] (Fe), was fully characterized by TEM, HRTEM, EDS element mapping and XPS. Then, its peroxidase-like activity was explored with Km of 1.74 mM and Vmax of 9.60 × 10 -8 M·S -1 towards H 2 O 2 , indicating excellent catalytic activity. Based on cascade reaction between choline oxidase and Au/ [email protected] (Fe), a quick colorimetric method was established for choline detection in infant formula milk powder. After comprehensive verification, this method presented the merits of simple operation, satisfied detection limit (0.05 mM), wide linear range (0.05–2.0 mM), high accuracy (recovery of 92.2%-105.2%) and nice selectivity. This colorimetric method was applied to the determination of choline in milk powders of five brands. Our study could offer valuable reference for finding highly efficient nanozyme and constructing novel optical biosensors.
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Analytical Methods
·
2022
·Vol. 14
·期 41
·4127-4132
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IF 3.532
Lei Jiang,Lin Yuan,Ze Liu,Yingying Xiang,Fei Song,Lifen Meng,Yujiao Tu
Colorant tartrazine is widely used in the food industry, but its long-term and excessive consumption is harmful to human health. Therefore, it is necessary to establish a sensitive detection method for tartrazine. Blue fluorescent carbon dots with L-arginine and o-phenylenediamine as precursors, namely L-Arg/oPD-CDs, were prepared via the hydrothermal method. Then, L-Arg/oPD-CDs were further purified by dialysis, thin layer chromatography and column chromatography. A dual-mode nanosensor based on fluorescent and UV absorption was successfully developed. Excellent linear ranges of 0–5 μM and 10–50 μM were obtained with a low detection limit of 42.3 nM based on fluorescence. A good linear range of 0–50 μM was obtained with a low detection limit of 130.15 nM based on UV absorption. The quenching mechanism of tartrazine towards L-Arg/oPD-CDs fluorescence was the inner filter effect. In addition, a dual-mode nanosensor was used for tartrazine determination in millet, maize flour, carbonated drink, and sugar samples. This study provides new insight into the detection of tartrazine by applying a dual-mode nanosensor.
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DYES AND PIGMENTS
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2022
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IF 5.122
Yingying Xiang,Yujiao Tu,Lei Jiang,Lin Yuan,Ze Liu,Qiang Xie,Xuequan Xiong,Fei Song
As one of the most widely used azo synthetic dyes, long-term consumption of excessive amaranthcan cause a threat to health. It is very urgent to establish high selective and sensitive method for amaranth detection. Orange fluorescent carbon dots utilizing l -methionine and o-phenylenediamine as raw materials, namely S,N-CDs, were prepared via one-step hydrothermal method . Dialysis, thin layer chromatography and chromatographic column were employed for further purification. The purified dual-mode nanoprobe based on fluorescent and UV absorption was successfully established. For fluorescent, excellent linear ranges of 5–70 μM and 110–195 μM were obtained, and low detection limit was 345.22 nM. For UV absorption, excellent linear ranges of 0–155 μM with low detection limit 12.70 nM. It revealed that inner filter effect and static quenching dominated in amaranth quenching the fluorescent of S,N-CDs. Furthermore, the dual-mode nanoprobe could be efficiently applied to determine amaranth in fruit juice, carbonated drink, and strawberry jam samples. It provided a new insight on the purification of carbon dots and detection of amaranth by dual-mode nanoprobe.
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INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Jinsong Wang,Farzad Seidi,Yang Huang,Huining Xiao
Incorporating corrosion inhibitors in the formulation of coatings can yield active coatings which are able to provide a sustained release of the corrosion inhibitor in the case of emergence of microcrack in the structure of the coating. Herein, we developed bio-based polyurethane sublayer coatings with strong anticorrosion performances. First, lignin-corrosion inhibitor (LCI) conjugate was obtained through conjugation of nicotinic acid to lignin through hydrolytically cleavable ester linkage to afford the steady release of low concentrations of corrosion inhibitor in case of the emergence of microcracks. Subsequently, solution polyaddition of LCI with diisocyanates and polypropylene glycol and coating on the stainless steel surface afford the polyurethane coatings. Coating with a thickness of 16 µm increased the E corr from − 481 mV (for bare steel) to + 187 mV and reduced both I corr and corrosion rate more than 6700 times. As an added benefit, after creating artificial cracks on the surface of the coating, the release of corrosion inhibitors gradually increased the impedance value, indicating a thin barrier layer has formed within the cracks.
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Analytical Methods
·
2021
·Vol. 13
·期 43
·5231-5239
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IF 2.896
Xuejia Chen,Tingkai Xiong,Jin Xu,Yan Li,Min Zhang,Ying Liang
In this paper, a novel and stable fluorescent reagent, quinoline-2,3-dicarbaldehyde (QDA), is synthesized as a probe to detect ammonium in natural water. Ammonium reacts with QDA in the presence of SO32− and Ca2+ to form a fluorescence product, which has maximum excitation and emission wavelengths at 429 nm and 518 nm. The concentration of reagents, the reaction temperature, the reaction time, and the pH in the final solution are investigated and optimized. The interferences of typical organic nitrogen and inorganic compounds are evaluated, and results prove that most volatile amines have little or negligible effect. Under the optimized conditions, this method provides a limit of detection of 0.065 μmol L−1, a calibration range of 0.216–9 μmol L−1, and reproducibility (with a relative standard deviation) of 1.9% for 1.5 μmol L−1 ammonium. For water sample analysis, the proposed method provides comparable results to those of the conventional o-phthalaldehyde method but has longer reagent stability (42 days).
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
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IF 4.098
Mengqi Huang,Changlun Tong
Cobalt as a transition metal ion is a biologically essential trace element, and plays an important role in various biological systems. The silicon nanoparticles (SiNPs) / gold nanoparticles (AuNPs) composite as a simple and efficient fluorescent probe was developed to detect Co 2+ and vitamin B 12 (VB 12 ) based on the selective aggregation and inner filter effect (IFE). The green-emitting SiNPs were synthesized by one-pot hydrothermal method, and the AuNPs were synthesized and modified with thioglycolic acid and cetyltrimethylammonium bromide. The fluorescent probe was fabricated by simple mixing the SiNPs and AuNPs together. In the presence of Co 2+ /VB 12 , AuNPs are selectively aggregated, which results in the enhancement of the local surface plasmon resonance absorption centered at 520 nm and the green fluorescence of SiNPs is significantly quenched via IFE. The fluorescence quenching efficiency of the probe is linearly proportional to the concentration of Co 2+ in the range of 0.1–80 µM with a low detection limit of 60 nM, which is far lower than the guideline value of Co 2+ in drinking water (1.7 µM). For vitamin B 12 (VB 12 ), its linear relationship is in the range of 0.1–100 µM, and the limit of detection is 69 nM. Furthermore, the proposed method shows good selectivity for the detection of Co 2+ and VB 12 , and does not need sophisticated pretreatment, only through simple filter. It has been applied in actual environmental water samples and drug tablets with satisfactory results.
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Chinese Medicine
·
2021
·Vol. 16
·期 1
·1-21
·
IF 5.455
Li Qiaofeng,Lan Taijin,He Songhua,Chen Weiwei,Li Xiaolan,Zhang Weiquan,Liu Ying,Zhang Qiuping,Chen Xin,Han Yaoyao,Su Zhiheng,Zhu Dan,Guo Hongwei
Background Lei-gong-gen formula granule (LFG) is a folk prescription derived from Zhuang nationality, the largest ethnic minority among 56 nationalities in China. It consists of three herbs, namely Eclipta prostrata (L.) L., Smilax glabra Roxb, and Centella asiatica (L.) Urb. It has been widely used as health protection tea for hundreds of years to prevent hypertension in Guangxi Zhuang Autonomous Region. The purpose of this study is to validate the antihypertensive effect of LFG on the spontaneously hypertensive rat (SHR) model, and to further identify the effective components and anti-hypertension mechanism of LFG.Methods The effects of LFG on blood pressure, body weight, and heart rate were investigated in vivo using the SHR model. The levels of NO, ANG II, and ET-1 in the serum were measured, and pathological changes in the heart were examined by H&E staining. The main active components of LFG, their corresponding targets, and hypertension associated pathways were discerned through network pharmacology analysis based on the Traditional Chinese Medicine Systems Pharmacology (TCMSP), Traditional Chinese Medicine Integrated Database (TCMID), and the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM). Then the predicted results were further verified by molecular biology experiments such as RT-qPCR and western blot. Additionally, the potential active compounds were predicted by molecular docking technology, and the chemical constituents of LFG were analyzed and identified by UPLC-QTOF/MS technology. Finally, an in vitro assay was performed to investigate the protective effects of potential active compounds against hydrogen peroxide (H2O2) induced oxidative damage in human umbilical vein endothelial cells (HUVEC).Results LFG could effectively reduce blood pressure and increase serum NO content in SHR model. Histological results showed that LFG could ameliorate pathological changes such as cardiac hypertrophy and interstitial inflammation. From network pharmacology analysis, 53 candidate active compounds of LFG were collected, which linked to 765 potential targets, and 828 hypertension associated targets were retrieved, from which 12 overlapped targets both related to candidate active compounds from LFG and hypertension were screened and used as the potential targets of LFG on antihypertensive effect. The molecular biology experiments of the 12 overlapped targets showed that LFG could upregulate the mRNA and protein expressions of NOS3 and proto-oncogene tyrosine-protein kinase SRC (SRC) in the thoracic aorta. Pathway enrichment analysis showed that the PI3K-AKT signaling pathway was closely related to the expression of NOS3 and SRC. Moreover, western blot results showed that LFG significantly increased the protein expression levels of PI3K and phosphorylated AKT in SHR model, suggesting that LFG may active the PI3K-AKT signaling pathway to decrease hypertension. Molecular docking study further supported that p-hydroxybenzoic acid, cedar acid, shikimic acid, salicylic acid, nicotinic acid, linalool, and histidine can be well binding with NOS3, SRC, PI3K, and AKT. UPLC-QTOF/MS analysis confirmed that p-hydroxybenzoic acid, shikimic acid, salicylic acid, and nicotinic acid existed in LFG. Pre-treatment of HUVEC with nicotinic acid could alleviate the effect on cell viability induced by H2O2 and increase the NO level in cell supernatants.Conclusion sLFG can reduce the blood pressure in SHR model, which might be attributed to increasing the NO level in serum for promoting vasodilation via upregulating SRC expression level and activating the PI3K-AKT-NOS3 signaling pathway. Nicotinic acid might be the potential compound for LFG antihypertensive effect.
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INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2021
·
IF 4.32
Yang Mu,Fuyin Zhang,Ning Li,Shanshan Pi,Ang Li,Qing Hong,Jian He,Jiguo Qiu,Jiandong Jiang
Dipicolinic acid (DPA) is a widely distributed pyridine derivative containing two carboxylate groups. However, the catabolism of DPA mediated by microorganisms are rarely reported. Alcaligenes faecalis strain JQ135, previously isolated in our lab, was able to degrade various pyridine derivatives, including nicotinic acid (NA). In this study, it was found that strain JQ135 could utilize DPA as the sole carbon and nitrogen source for growth and degrade 1.8 mM (300 mg/L) DPA to a non-detectable level within 12 h. The catabolism of DPA by strain JQ135 extracted two intermediates, i.e., 3-hydroxy-2,6-dipicolinic acid and 4-imino-pent-2-enedioic acid. Moreover, deletion of the key genes essential for NA catabolism (such as moaCDEBmoeA and maiA ) did not affect DPA catabolism, indicating that the maleamate pathway and molybdenum cofactor are not involved in the DPA catabolism by strain JQ135. This study will provide insights into the bacterial catabolism of DPA.
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Journal of Bionic Engineering
·
2021
·Vol. 19
·期 1
·188-196
·
IF 2.682
Wang Qiaoying,Cai Jiali,Xu Zihan,Huang Shihui,Zhou Ruiping,Wang Zhiyong
Chemotherapy is still a kind of important strategy for cancer treatment, but lacking effective delivery system limits the therapeutic outcome. Owing to the excellent biocompatibility, albumin has been employed as drug vehicle; however, it has to face complicated synthesis procedures and still needs an effective technology for the drug delivery evaluation. In this study, a facile method was utilized to prepare a protein-based theranostic system through the self-assembly of albumins with small molecules and drugs; furthermore, the paramagnetic divalent manganese ions were conjugated on the surface of the nanocomposite through a coordination bond and carried out the magnetic resonance imaging. This nanosystem with a stable structure exhibited about 80 nanometer size and 7.7 [Mn] mM -1 S -1 T 1 relaxivity; moreover, the pH-sensitive drug releasing property and the imaging function empower it with theranostic cancer therapy. Moreover, the nanocomposites effectively entered into the cancer cells and showed superior antitumor ability; and these treated cells exhibited significant T 1 signal. These results suggested that the albumin-based nanosystem is a novel chemotherapy drug delivery vehicle and a promising candidate for magnetic resonance imaging.
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Molecular Catalysis
·
2021
·
IF 5.062
Yu-Ting Shang,Jiufu Qin,Jin-Song Gong,Zi-Kai Wang,Heng Li,Hui Li,Jin-Song Shi,Zheng-Hong Xu
A nicotinate dehydrogenase producing strain was isolated and identified to be Pseudomonas putida , which could bio-transform nicotinic acid into 6-hydroxynicotinic acid. The activity of nicotinate dehydrogenase towards nicotinic acid could reach up to 1.11 U/mL and the yield of 6-hydroxynicotinic acid accumulated to 176 g/L within 30 h with a space time yield of 1.36 g/(g•h). In order to further improve its catalytic potential, a mutant library was constructed through atmospheric and room temperature plasma (ARTP) and rapidly screened by a high-throughput screening strategy, which obtained a mutant strain mut-5 with activity of 2.68 U/mL. Via developing a nitrilase-nicotinate dehydrogenase enzymatic cascade system, 54.5 g/L 6-hydroxynicotinic acid could be obtained within 585 min with 3-cyanopyridine as the substrate, which would further reduce the production costs. The results indicated that the nicotinate dehydrogenase (separately or combining with nitrilase) could serve as a superior catalyst for 6-hydroxynicotinic acid production. To our knowledge, the nicotinate dehydrogenase activity reported in this study was the highest level in literature to date.
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