Food Chemistry-X
·
2024
·
IF 6.5
Wentao Qian,Xiaobing Wang,Hongliang Li,Yinhua Zhu,Pengjie Wang,Xiaolu Geng,Jinhui Yang,Huiyuan Guo,Menghui Wang,Chong Chen
Introducing Holstein cows on Qinghai-Tibetan Plateau is a potential solution to enhance local milk production. However, the relationship between milk quality and altitude in China remains unknown. Therefore, the components and plasmin (PL) system of raw milk from different altitudes (sea level, 1600, 2700, and 3800 m) were investigated. The daily milk production of Holstein cows and PL activity decreased as the altitude increased. However, the components content of raw milk, plasminogen (PLG)/PL ratio, activities of PLG and plasmin activator (PA) increased with altitude. The pasteurization resulted a significant decrease in PA activity of all milk and a significant increase in PL activity in milk collected at higher altitudes (2700 and 3800 m), suggesting the pasteurization was unsuitable for preserving milk at higher altitudes. This study offered references for the production and storage of milk after introducing Holstein cows on Qinghai-Tibetan Plateau.
研究领域:
农业
·
乳品科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yimeng Ren,Longzhu Zhou,Chaohua Tang,Jing Li,Yujie Shi,Qingyu Zhao,Junmin Zhang,Yueyu Bai
Jiaxian Red cattle is an indigenous, high quality cattle breed with marbled beef in China. We investigated the changes in lipid distribution, volatile compound and taste in Biceps femoris , Supraspinatus and Longissimus dorsi of Jiaxian Red cattle to further explore the quality characteristics of beef muscles with high lipid deposition. Neutral lipids in high-lipid-depositing beef had a dominant content percentage; in addition, 24 triglyceride species varied significantly. Longissimus dorsi lipid was more non-polar, with the highest total lipid and the lowest phospholipid content. The distribution of neutral lipids and glycerophospholipids was analyzed. Triglyceride was the lipid class that had the highest monounsaturated fatty acid percentage (52.44 %-55.88 %). Saturated and mono/polyunsaturated fatty acid classes content derived from neutral lipid increased in Longissimus dorsi , while that derived from glycerophospholipid increased in Supraspinatus. N-3 fatty acids content had low levels in Longissimus dorsi. Triglycerides containing oleic acid and linoleic acid had a significant positive correlation with differential aldehydes. These findings provide valuable insights into the lipid profile of beef with high lipid deposition, offering a foundation for future enhancements in beef quality.
研究领域:
食品科学
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动物学
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生物化学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yuanfeng Zhao,Jingrui Zhou,Wenju Luo,Lingling Jiang,Fuzhan Song,Jiang Ran,Haoxiang Xu,Lu Lei,Rong Ai,Bo Yu
This study investigated breed-specific flavor characteristics in Guanling (GL), Sinan (SN), and Simmental-crossbred (XZ) cattle using UHPLC-MS/MS and GC × GC-TOF MS. Specifically, XZ had 73 and 48 differential metabolites compared to GL and SN, respectively, whereas GL and SN showed 31 discriminated metabolites. Notably, these differences were linked to flavor-related metabolic pathways, particularly beta-alanine, histidine, glutathione, and purine metabolism. Regarding the composition of volatile organic compounds (VOCs), GL displayed a higher ketone content (18.58 %), whereas XZ was enriched in alcohol (56.25 %). Key VOCs with the highest flavor contributions across all breeds were 2,3-Butanedione, (E)-2-nonenal, 2-ethyl-1-hexanol, and (E)-2-octenal. However, dimethyl sulfide contributed significantly to GL and SN flavors but not to XZ. Collectively, the 49 differential VOCs led to aroma profiles of breeds varied in “sweet”, “fruity”, “nutty”, and “waxy” characteristics. This comprehensive analysis of non-volatile metabolites and flavor-related VOCs revealed breed-specific profiles and crossbreeding effects, providing insights for improving meat quality.
研究领域:
食品科学
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畜牧学
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分析化学
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代谢组学
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风味化学
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产品关联
DOI
PubMed
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2022
·
IF 2.382
Xiong Chen,HongGao Liu,JieQing Li,YuanZhong Wang
Wild boletes, as a nutritious food extensively used in food fields. It is rich in polyphenolic compounds, which is it can promote human health, improve physical performance and reduce the risk of developing diseases. But the existing methods could not evaluate the total polyphenol content (TPC) quickly and accurately. In this study, two-dimensional correlation spectroscopy (2D-COS) images were generated by high performance liquid chromatography (HPLC) and Fourier transform near-infrared (FT-NIR) spectroscopy using a generalized two-dimensional correlation algorithm. In addition, the TPC of all samples was determined. Residual convolutional neural network (ResNet) models were then established to identify different levels of TPC. The results shown that compared with HPLC, FT-NIR combined with 2D-COS algorithm had excellent identification performance and greatly reduces the time of data processing. The accuracy rates of training sets and testing sets were 100%. The established method can be effectively applied to Lanmaoa asiatica , Butyriboletus roseoflavus and Rugiboletus extremiorientalis . This work provides a novel and comprehensive strategy for other nutritious foods.
研究领域:
食品科学
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分析化学
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机器学习
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1139
·
IF 5.1
Liusha Kuang,Enqi He,Lei Zhou,Aihua Lou,Yan Liu,Wei Quan,Qingwu Shen
Xiangxi Yellow Cattle, an indigenous Chinese livestock breed recognized for its exceptional nutrient composition and superior meat characteristics, has gained significant market preference among consumers. This investigation focused on evaluating physicochemical attributes, flavor development patterns, and bacterial population dynamics in chilled beef samples stored at 4 °C over an 8-day period. The research further examined interrelationships between meat parameters, predominant microbial species, and crucial aroma-active substances. Findings revealed a progressive elevation in lipid/protein oxidation levels, biogenic amine accumulation, and TVB-N values as microbial proliferation intensified toward the late storage phase. Microbial analysis demonstrated a final total viable count of 7.17 log10 (CFU/g), with bacterial community dominance distributed amongFirmicutes(58.15%),Proteobacteria(29.56%), andBacteroidota(12.05%). Notably,Brochothrix thermosphactaemerged as the primary spoilage organism in terminal storage phases. Volatile organic compounds (VOCs) in the beef during storage were analyzed, with a total of 66 compounds identified. The critical analysis identified 2,3-butanedione and 2-butanone as microbial metabolism-dependent substances showing strong correlations with meat quality parameters, emerging as freshness markers for beef evaluation. Importantly, the study highlighted the necessity for deeper investigation into microbial–VOC interactions, particularly considering the intricate bacterial ecosystems in aquatic environments. These outcomes enhance our understanding of spoilage mechanisms in Xiangxi Yellow Cattle beef while proposing practical approaches for microbial control in meat preservation systems.
研究领域:
食品科学
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微生物学
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动物科学
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产品关联
DOI
PubMed
高影响因子
Microfluidics and Nanofluidics
·
2019
·Vol. 23
·期 5
·1-9
·
IF 2.437
Yang Xiaohan,Weldetsadik Netsanet Tesfaye,Hayat Zafar,Fu Taotao,Jiang Shaokun,Zhu Chunying,Ma Youguang
The aim of this work was to experimentally examine apparent rheological properties of fluids in microchannels based on pressure drop. Pressure drops of Newtonian and non-Newtonian fluids in microchannels were measured using pressure sensors. The effects of flow rate, Reynolds number, and viscosity of fluids on the pressure drop of single phase flow in microchannels were investigated, and the measurements of pressure drop were compared with prediction models. For Newtonian fluids, the friction factor in the laminar regime is f/ 2 = 26/ Re . The results show that the pressure drop of Newtonian fluid is in good agreement with the prediction formula proposed by Cornish. However, the pressure drop of non-Newtonian fluid deviates from the theoretical prediction. A power-law is proposed for the relationship between the apparent viscosity of non-Newtonian fluid and the characteristic shear rate in a microchannel based on the pressure drop measurement. This relationship between the apparent viscosity and the characteristic shear rate in a specific microchannel is completely different from the rheological curve measured by cone-and-plate rheometer, due to the heterogeneous distribution of shear rates at the radial direction for non-Newtonian fluids flowing in a microchannel.
研究领域:
流体力学
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微流控技术
·
工程学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinjin Tong,Zezheng Liu,Kexuan Zhou,Kaiming Wang,Shijiao Guo,Hua Zhang
The overuse of antibiotics in livestock production poses a significant threat to public health, primarily by accelerating the emergence of antibiotic-resistant bacteria—a critical global issue. Therefore, the development of effective alternatives to antibiotics is urgently required to address this problem. In this study, BLfcin-NPs@CS/β-GP thermosensitive hydrogels were developed by incorporating bovine lactoferricin-loaded chitosan nanoparticles into a chitosan/β-GP hydrogel matrix. Scanning electron microscopy (SEM) revealed the formation of a three-dimensional porous structure. Fourier-transform infrared (FTIR) spectroscopy confirmed successful synthesis, while X-ray diffraction (XRD) analysis indicated that nanoparticle incorporation did not alter the crystallinity of the hydrogel. The rheological properties, in vitro degradation, biocompatibility, and antibacterial activity of the composite hydrogels were systematically evaluated, and the results demonstrated that the composite hydrogels exhibited temperature sensitivity, enhanced degradation rates, low cytotoxicity, and strong antibacterial activity. These findings suggest that BLfcin-NPs@CS/β-GP hydrogels present a promising alternative to antibiotics for treating bacterial infections, such as bovine mastitis, offering an innovative and effective strategy to reduce antibiotic use and mitigate the risk of antibiotic resistance in livestock production.
研究领域:
兽医学
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生物材料学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Yuan Xu,Yuhai Tang,Yayun Zhao,Ruixia Gao,Junjie Zhang,Delai Fu,Zhaolun Li,Hecheng Li,Xiaoshuang Tang
Novel magnetic molecularly imprinted nanomaterials (DA + BSA-MMIPs) were prepared adopting bovine serum albumin (BSA) and dopamine as bifunctional monomers for the first time. Besides the role of assistant functional monomer, BSA can exclude the proteins with like charges and promote low molecular weight tetracyclines to be adsorbed. Thus, the DA + BSA-MMIPs could fulfil the selective separation of tetracyclines directly from milk samples. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of uniform imprinting layers, stable crystalline phase, and good magnetism of the DA + BSA-MMIPs, they have rapid binding kinetic, high adsorption capacity, and favorable reusability. The imprinted nanomaterials were coupled with HPLC to selectively extract and determine trace tetracyclines from untreated milk samples. The recoveries of tetracyclines are in the range of 84.1–95.8% with relative standard deviations of less than 6.7%. The developed method is especially suitable for the selective enrichment and detection of target compounds directly from a complex sample with proteins.
研究领域:
分析化学
·
环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
RESEARCH IN VETERINARY SCIENCE
·
2023
·
IF 2.4
Jirao Shen,Feng Yang,Guibo Wang,Xiaoqing Mou,Jinyu Li,Xuezhi Ding,Xurong Wang,Hongsheng Li
Staphylococcus haemolyticus ( S. haemolyticus ) is one of the most common coagulase-negative staphylococci (CoNS) isolates from bovine mastitis . Paeoniflorin (PF) shows anti-inflammatory effects on different inflammatory diseases in vitro studies and in vivo animal experiments. In this study, the viability of bovine mammary epithelial cells (bMECs) was detected by the cell counting kit-8 experiment. Subsequently, bMECs were induced with S. haemolyticus , and the induction dosage was determined. The expression of pro-inflammatory cytokines and toll-like receptor (TLR2) and nuclear factor kappa-B (NF-κB) signaling pathway-related genes were investigated by quantitative real-time PCR. The critical pathway proteins were detected by western blot . The results showed that the multiplicity of infection (MOI; the ratio of bacteria to bMECs) 5:1 of S. haemolyticus for 12 h could cause cellular inflammation, which was selected to establish the inflammatory model. Incubation with 50 μg/ml PF for 12 h was the best intervention condition for cells stimulated by S. hemolyticus . Quantitative real-time PCR and western blot analysis showed that PF inhibited the activation of TLR2 and NF-κB pathway-related genes and the expression of related proteins. Western blot results showed that PF suppressed the expression of NF-κB unit p65, NF-κB unit p50, and MyD88 in bMECs stimulated by S. haemolyticus . The inflammatory response pathway and molecular mechanism caused by S. haemolyticus on bMECs are related to TLR2-mediated NF-κB signaling pathways. The anti-inflammatory mechanism of PF may also be through this pathway. Therefore, PF is expected to develop potential drugs against CoNS-induced bovine mastitis.
研究领域:
兽医学
·
免疫学
·
药理学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yimeng Geng,Sizhe Zhang,Yuxian Wang,Nengsheng Ye,Yuhong Xiang
We developed a fluorescent switching based on bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs) and kanamycin (Kana) aptamer to detect Kana. Red fluorescence of BSA-AuNCs was turn off when Kana aptamer was assembled on the surface of BSA-AuNCs. However, after adding Kana, the specific aptamer detached from the surface of the BSA-AuNCs and formed the complex of aptamer-kanamycin, then the red fluorescence of BSA-AuNCs was turn on. The fluorescence intensity of BSA-AuNCs can be mediated by the amount of Kana aptamer. A good linear response for Kana was obtained in the concentration range 0.04 nM to 7.0 nM. The assay was used to detect Kana in milk matrix, and the accuracy and recovery of the method were satisfied. It is worth noting that the aptamer mediated BSA-AuNCs fluorescent switching was simple and easy to fabricate, which can provide a novel assay for the detection of small molecules.
研究领域:
分析化学
·
纳米技术
·
食品安全
✓ 已验证
DOI
高影响因子
Animal Production Science
·
2018
·Vol. 59
·期 3
·471-478
·
IF 1.371
C. Wang,Q. Liu,G. Guo,W. J. Huo,Y. X. Wang,Y. L. Zhang,C. X. Pei,S. L. Zhang,C. Wang,Q. Liu,G. Guo,W. J. Huo,Y. X. Wang,Y. L. Zhang,C. X. Pei,S. L. Zhang
The objective of the present study was to evaluate the effects of fibrolytic enzymes (FE, containing 160 units of cellulase and 4000 units of xylanase) or isobutyrate (IB) supplementation on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in dairy calves. Forty-eight Holstein bull calves of 15 days of age and of 44.9 ± 0.28 kg of BW were randomly assigned to four groups in a 2 × 2 factorial arrangement. Two levels of FE (0 g (FE–) or 1.83 g per calf per day (FE+)) and IB (0 g (IB–) or 6 g per calf per day (IB+)) were added. Calves were weaned at 60-day-old and four calves were selected from each treatment at random and slaughtered at 45 and 90 days of age. There was no IB × FE interaction effect. Ruminal pH decreased with IB or FE supplementation for post-weaned calves, whereas concentrations of total volatile fatty acids and acetate increased with IB or FE supplementation for pre- and post-weaned calves. Acetate to propionate ratio increased with IB supplementation, but was unaffected by FE supplementation. Ammonia-N concentration decreased with IB or FE supplementation for pre- and post-weaned calves. For post-weaned calves, activities of CMCase increased with IB or FE supplementation, and activities of cellobiase, xylanase, pectinase, β-amylase and protease increased with IB supplementation. Populations of B. fibrisolvens and F. succinogenes for pre- and post-weaned calves and R. flavefaciens for post-weaned calves increased with IB or FE supplementation. It is suggested that ruminal fermentation and growth performance of calves was improved with IB and FE supplementation, and the combination of IB and FE has the potential to stimulate the growth of pre- and post-weaned dairy calves.
研究领域:
动物科学
·
微生物学
·
营养学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Youyuan Lu,Jing Wang,Donghua Yu,Yong-Jie Yu,Kai He,Lanping Guo,Luqi Huang,Hanqing Wang
Bovis calculus has been used as a traditional Chinese medicine for more than 2000 years. Due to Natural Bovis calculus (NBC) is expensive and rare. To meet the clinical needs, three kinds of Bovis calculus substitutes, namely Bovis calculus sativus (BCS), Bovis calculus artifactus (BCA), and Bovis calculus culture (BCC) were approved to be used. To illustrate the characterization of metabolite landscape of the Bovis calculus sources, untargeted metabolomics by UHPLC-HRMS was carried out on four sources of Bovis calculus. The results showed that a total of 12,643 features were observed in Bovis calculus samples. The four Bovis calculus sources could be clearly discriminated based on 6262 significant difference features according to PCA and PLS-DA. NBC and BCA samples showed different metabolites characterization. BCS and BCC samples showed similarity with each other. The quality of BCC was affected by producers. Heat map analysis results showed that identified 43 chemical compounds can be used as marker to classified Bovis calucus sources. These compounds mainly affected bile acid biosynthesis, betaine metabolism, and taurine and hypotaurine metabolism. As we know, this is the first comprehensive comparison of the metabolite difference among NBC, BCS, BCC and BCA based on untargeted metabolomics combined with chemometric. The results illustrated that the material basis of the four Bovis calculus source was not consistent, it correlated with the producing process. BCS and BCC may be used to replace each other. It provides a theoretical basis for its clinical application.
研究领域:
中药学
·
代谢组学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）