Journal of Functional Biomaterials
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2022
·Vol. 14
·期 1
·15
·
IF 4.901
Lixin Long,Yang Song,Xiaoyi Tian,Lanyue Cui,Chengbao Liu,Shuoqi Li,Yu Wang,Rongchang Zeng
Photoactivated sterilization has received more attention in dealing with implant-associated infections due to its advantages of rapid and effective bacteriostasis and broad-spectrum antibacterial activity. Herein, a micro-arc oxidation (MAO)/polymethyltrimethoxysilane (PMTMS)@hemin-induced calcium-bearing phosphate microsphere (Hemin-CaP) coating was prepared on pure magnesium (Mg) via MAO processing and dipping treatments. The morphology and composition of the coating were characterized via scanning electron microscopy, Fourier transform infrared spectrometer, X-ray diffractometer and X-ray photoelectron spectrometer. Corrosion behavior was evaluated through electrochemical and hydrogen evolution tests. The release of Fe3+ions at different immersion times was measured with an atomic absorption spectrophotometer. Antibacterial performance and cytotoxicity were assessed using the spread plate method, MTT assay and live/dead staining experiment. The results showed that the corrosion current density of the MAO/PMTMS@(Hemin-CaP) coating (4.41 × 10−8A·cm−2) was decreased by two orders of magnitude compared to that of pure Mg (3.12 × 10−6A·cm−2). Photoactivated antibacterial efficiencies of the Hemin-CaP microspheres and MAO/PMTMS@(Hemin-CaP) coating reached about 99% and 92%, respectively, which we attributed to the photothermal and photodynamic properties of hemin with a porphyrin ring. Moreover, based on the release of Fe3+ions, the MC3T3-E1 pre-osteoblasts’ viability reached up to 125% after a 72 h culture, indicating a positive effect of the coating in promoting cell growth. Thus, this novel composite coating holds a promising application as bone implants.Keywords:magnesium;degradable biomaterial;antibacterial coating;biocompatibility;hemin
研究领域:
生物医学工程
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材料科学
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DOI
PubMed
高影响因子
Acta Biomaterialia
·
2023
·
IF 9.7
Guangdong Sun,Xiuling He,Meilin Feng,Xia Xu,Jine Chen,Yongqiang Wang
Visible light-induced photocrosslinking techniques have attracted significant attention for their flexibility, controllability, safety, and energy conservation, especially in tissue engineering and biofabrication, compared to UV photocrosslinking. Despite these advantages, current photoinitiators are constrained by various challenges, including inadequate photoinitiation efficiency, low biocompatibility, poor water solubility, and limited compatibility with diverse crosslinking systems. Here, a water-soluble derivative of riboflavin, flavin mononucleotide (FMN − ), was used to assess its potential as an initiator in multiple-photocrosslinking systems, including radical photopolymerization, dityrosine, and ditryptophan coupling crosslinking, under blue light irradiation. Blue light irradiation facilitated an efficient electron transfer reaction between FMN − and persulfate, owing to their suitable spectral compatibility and photoactivity. The resulting oxidizing free radicals and excited triplet state of FMN − served as initiating active species for the multiple-photocrosslinking reactions. The combination of FMN − and potassium persulfate (KPS) exhibited exceptional photoinitiation efficiency for various biomaterials, including silk fibroin, gelatin, poly(ethylene glycol) diacrylate, and carboxymethyl cellulose modified with amino acids. Furthermore, the cytocompatibility of the FMN − /KPS photoinitiator was demonstrated by the survival rates of 3T3-LI fibroblasts encapsulated in it, which exceeded 95 % when compared to a commercial initiator. Statement of significance By introducing persulfate, the photoinitiation efficiency of flavin mononucleotide was significantly improved. The application scenarios of flavin mononucleotide and persulfate combinations were also greatly extended, including radical photopolymerization, dityrosine, diphenylalanine, and ditryptophan coupling crosslinking. Among them, the coupling crosslinking of amino acids (di-phenylalanine, and di-tryptophan) modified carboxymethyl cellulose, to our knowledge, was first reported. The excellent cytocompatibility of cell encapsulation further proved that the combinations of flavin mononucleotide and persulfate have great potential in tissue engineering.
研究领域:
生物医学工程
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组织工程
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生物材料科学
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产品关联
DOI
PubMed
高影响因子
International Journal of Precision Engineering and Manufacturing
·
2017
·Vol. 18
·期 5
·755-761
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IF 1.497
Chen Zhichao,Zhang Xianglin,Chen Penghua,Li Wenchao,Zhou Kui,Shi Lei,Liu Kang,Liu Changqing
Recently, additive manufacturing is one of the most focused research topics due to its explosive development, especially in manufacturing engineering and medical science. In order to build 3D complex scaffolds with multi-biomaterials for clinical application, a new 3D multi-nozzle system with dual-mode drives, i.e. ejection and extrusion was developed. In this paper, much effort was made to gain fine control of droplet and excellent coordination during fabrication. Specifically, the parameters that influence the size and stability of droplet most was intensively studied. Considering that the biomaterials used in the future may have much difference in properties, the combination of parameters was investigated to facilitate the settings for certain-sized droplets, which are potentially eligible for bio-printing. The dispensing nozzles can work well both in independent and convergent mode, which can be freely switched. Outstanding to the most currently used 3D bio-printing techniques, this system can fabricate scaffolds with multimaterials of both low viscosity (by pneumatic dispensing) and high viscosity (through motor extrusion). It is highly expected that this system can satisfy clinical application in the near future.
研究领域:
制造工程学
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医学科学
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生物打印技术
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DOI
高影响因子
Journal of Water Process Engineering
·
2025
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IF 6.7
Kang Zhichao,Wang Tianye,Nannan Li,Han Xuerong,Wang Chenxu,Yang Xiutao,Jing Yue,Yu Hongwen
The leaching of atrazine wastes from agricultural areas presents a considerable risk to the ecosystem. This work aims to create microorganism-material systems employing the atrazine-degrading strain Paenarthrobacter sp. KN0901 and C 3 N 5 -based materials to efficiently eliminate atrazine residues from aquatic environments at low temperatures. The synthesized C 3 N 5 and O-C 3 N 5 markedly improved the growth and atrazine degradation of KN0901. The degradation rate of atrazine in the KN0901-C 3 N 5 and KN0901-O-C 3 N 5 systems rose by 7.3 % and 15.1 %, respectively, in comparison to biodegradation systems. The elevated NADH/NAD+ and GSH/GSSG ratios indicated a greater availability of electrons to KN0901 when associated with C3N5-based materials, implying that these materials contributed electrons to KN0901. Nitazoxanide and methyl viologen inhibited the electron transfer route, leading to a significant decrease in the breakdown rate of KN0901-C 3 N 5 systems. Glucose and sodium citrate, functioning as electron donors, markedly accelerated the degradation rate of atrazine in the KN0901-C 3 N 5 systems. This work demonstrated that C 3 N 5 -based materials may donate electrons to KN0901, hence improving the efficiency of atrazine breakdown at low temperatures. This work successfully developed microorganism-material systems that effectively eliminate atrazine in aquatic settings, especially in cold climates.
研究领域:
环境生物学
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微生物学
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材料科学
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产品关联
DOI
高影响因子
Nature Protocols
·
2024
·
IF 13.1
Wu Yueming,Chen Kang,Wang Jiangzhou,Dai Wenhui,Yu Haowen,Xie Xinyi,Chen Minzhang,Liu Runhui
Synthetic polypeptides, also known as poly(α-amino acids), have the same polyamide backbone structures as natural proteins and peptides. As an important class of biomaterials, polypeptides have been widely used because of their biocompatibility, bioactivity and biodegradability. Ring-opening polymerization of N -carboxyanhydride (NCA) is a classical and widely used method for the synthesis of polypeptides. The dominantly used primary amine–initiated NCA polymerization can yield well-defined polymers and complex macromolecular architectures, but the reaction is slow and sensitive to moisture, making it necessary to use anhydrous solvents and a glovebox. One solution is to use lithium hexamethyldisilazide (LiHMDS) as the initiator, as described in this protocol. LiHMDS-initiated NCA polymerization is less sensitive to moisture and can be carried out in an open vessel outside the glovebox. It is also very fast; the reaction can be complete within 5 min to produce 30-mer polypeptides. In this protocol, poly(γ-benzyl- l -glutamate) is prepared as an example, but the protocol can easily be adapted to the synthesis of other polypeptides by generating NCAs from different amino acids, making it particularly suitable for the efficient parallel synthesis of polypeptide libraries. We provide detailed procedures for NCA synthesis and purification, the method of polymer end-group modification and measurement of polymerization kinetics and reactivity ratio. The procedure for synthesis of monomers and polymerization to form polypeptides requires <1 d. The superfast and open-vessel NCA polymerization method described here will probably enable a wide range of applications in the synthesis and functional study of polypeptide biomaterials.
研究领域:
生物化学
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高分子化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2018
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IF 6.735
Yue Sun,Yuanli Liu,Zimo Lou,Kunlun Yang,Dan Lv,Jiasheng Zhou,Shams Ali Baig,Xinhua Xu
Iron sulfide (FeS) nanoparticles with large specific surface area and abundant pore structure have been recognized as effective Hg(II) adsorbents. However, bare FeS nanoparticles can aggregate easily, which greatly limited their engineering applications. In this study, FeS nanoparticles were stabilized by biomaterials, including sodium carboxymethyl cellulose (CMC), gelatin and starch, and thoroughly investigated for the stabilization effects. Results demonstrated that the three biomaterial stabilized FeS nanoparticles namely CMC-FeS, gelatin-FeS and starch-FeS enhanced the adsorption efficiency significantly. The maximum adsorption capacities of CMC-FeS, gelatin-FeS and starch-FeS (mass ratio of stabilizer to FeS was 1:6) achieved ∼1726 mg/g, ∼1939 mg/g and ∼1989 mg/g respectively, which were over twice of the bare FeS. TEM images suggested that biomaterial-FeS nanoparticles were dispersed more uniformly than bare FeS. The removal processes of the three materials obeyed pseudo-second-order kinetic model ( R 2 ≥ 0.9986), implying that the rate-limiting step was the chemical sorption process. High removal efficiency of biomaterial-FeS nanoparticles was observed in the initial pH range of 6–11. The presence of Cl − could accelerate the reaction process, whereas the presence of humic acid (HA) could inhibit Hg(II) uptake. In addition, high concentration of coexisting cations (30 mM as Cd 2+ , Pb 2+ , Cu 2+ , and Ca 2+ ) had no significant effect on Hg(II) removal.
研究领域:
环境科学
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材料科学
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化学工程
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2019
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IF 3.45
Yufei Tang,Lei Chen,Zihao Duan,Kang Zhao,Zixiang Wu
The introduction of BaTiO 3 (BT) particles into biomaterials, such as polymethyl methacrylate (PMMA) bone cement , is among the effective ways to improve their osteoinductivity. However, excessive addition leads to mechanical degradation . An important problem to solve is the manner by which piezoelectric properties matching human bones can be obtained under the premise of sufficient mechanical properties . The piezoelectric effect is obtained by adding BT particles into PMMA bone cement. The piezoelectric coefficient close to the human bone is obtained at a relatively low BT addition amount by adding graphene on this basis. High mechanical properties can also be obtained. Graphene increases the piezoelectric coefficient by increasing the conductivity, dielectric constant, and effective polarization voltage of graphene (G)/BT(BaTiO 3 )/PMMA bio-piezoelectric composites. The compression strength of the aforementioned composites increases from 83.5 MPa to 89.5 MPa after the addition of 0.5 vol% graphene, and the requirements of bone implant materials were met. G/BT/PMMA bio-piezoelectric composites have no cytotoxicity, and graphene can also promote cell adhesion and proliferation on the composite's surface. The polarized bio-piezoelectric composites can improve cell morphology and promote cell proliferation . The number of cells increases with increasing piezoelectric coefficient. The composite has potential application prospects in various fields, such as oral cavity and bone implant.
研究领域:
生物医学工程
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材料科学
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产品关联
DOI
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
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2021
·Vol. 306
·期 11
·2100370
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IF 4.367
Xin Qi,Enyun Xu,Manman Jia,Jichuan Zhang,Liqun Zhang,Dongmei Yue
At present, the synthesis of body temperature triggering shape memory polymers usually requires elaborate structural design, which limits their wide application. Herein, starting from bio-based Eucommia ulmoides gum (EUG), a series of EUG/silica hybrids (ESHs) are prepared through a facile one-pot process, in which EUG is epoxied and then self-crosslinked with SiO 2 by epoxy ring-open reaction. Varying the amount of H 2 O 2 , the shape memory transition temperature ( T trans ) of ESHs is adjusted to 47.4–36.6 ℃, which is close to human body temperature (37 ℃). Among them, ESH-17 exhibited the best body temperature triggering shape memory ability ( T trans = 36.6 ℃), which can restore the permanent shape within 60 s at 37 ℃ with a shape fixity ratio of 99% and shape recovery ratio near 100%. In addition, the shape memory mechanism is discussed and shows some application scenarios of ESHs. The as-produced materials can be used as smart biomaterials such as self-tightening sutures, self-sealing root canal filling materials, and so on.
研究领域:
材料科学
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生物学
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医学
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产品关联
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2019
·Vol. 27
·期 2
·503-513
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IF 1.947
Zhou Chun Hui,Xia Shu Ting,Komarneni Sridhar,Kabwe Freeman Bwalya,Jin Gui Chen,Chu Mao Quan
Dolomite is an abundant, naturally occurring carbonate mineral, but the conventional processes of converting dolomite to new materials are time-consuming and energy-intensive. In addition, products from dolomite such as magnesium oxide, magnesium carbonate, magnesium hydroxide, which are used as adsorbents and additives, are mostly low value-added. Here, we demonstrated the conversion of dolomite to a mesoporous calcium-magnesium silicate (m-CMS) using a green and efficient continuous-flow synthesis method. The samples were characterized using powder X-ray diffraction, Fourier transformed infrared spectroscopy, N 2 adsorption/desorption isotherms, thermogravimetric analysis, scanning electron microscopy and transmission electron microscopy. The material possessed mesoporosity and exhibited high a specific surface area of 629 m 2 /g and a pore volume of 0.66 cm 3 /g. The maximum water absorptivity of the sample was 52.6%. After the m-CMS immersing in Tris–HCl solution for 56 days, the weight loss ratio reached 30 wt%, indicating its good potential biodegradability. Hydroxyapatite was formed on the surfaces after the m-CMS was immersed in simulated body fluids. The m-CSM provided nucleation sites, and subsequently supplied Ca 2+ for hydroxyapatite crystal growth, indicating that the material has potential bone conduction capability. This work suggests that m-CMS can be synthesized from dolomite and tetraethyl orthosilicate through a quick continuous process, and that the m-CMS could be used as a biomaterial.
研究领域:
生物医学工程
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材料科学
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生物材料学
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矿物加工
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绿色化学
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 7
·1150
·
IF 5.3
Qiuyu Wang,Jianwei Ma,Shaojuan Chen,Shaohua Wu
Electrospinning has contributed substantially to the construction of nanofibrous scaffolds for potential tissue engineering and regenerative medicine applications. However, conventional electrospinning only has the ability to generate and collect nanofiber scaffolds with a randomly oriented fibrous pattern, which lack the necessary cell alignment guidance function. In this study, a novel electrospinning fiber-collecting device was designed and developed by setting a series of small pin-ring-structured collectors on a large plain plate. Specifically, we demonstrated that the pin-ring-structured collectors, which were constructed by inserting a metal pin into the center of a metal ring, could collect the as-electrospun nanofibers with radially oriented structures in an innovative manner. We first investigated the suitable polymeric concentration for electrospinning poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and the optimum electrospinning concentration of PHBV was found to be 12% (w/v) PHBV dissolved in hexafluoroisopropyl alcohol (HFIP). Then, 12% (w/v) PHBV solution was electrospun into radially oriented nanofiber scaffolds using our novel electrospinning strategy, and their various performances were further compared with conventionally randomly oriented nanofiber scaffolds that were also produced from 12% (w/v) PHBV solution. The results showed that the radially oriented PHBV nanofiber scaffolds exhibited obviously enhanced mechanical properties and decreased hydrophobicity compared with the randomly oriented PHBV nanofiber scaffold controls. Importantly, the biological properties of radially oriented PHBV nanofiber scaffolds were also demonstrated to be enhanced, compared with randomly oriented PHBV nanofiber scaffolds, by effectively inducing cell alignment and significantly promoting cell proliferation. In sum, the present study indicates that our as-prepared nanofiber scaffolds with a radially oriented pattern are of great interest for advanced applications, such as wound dressings and tissue-engineered scaffolds.Keywords:electrospinning;nanofiber scaffold;fiber alignment;biocompatibility;biomaterials
研究领域:
生物医学工程
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组织工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2023
·
IF 13.1
Hua Zeng,Zheng-Ran Ying,Xiong Luo,Shuo Tan,Xiao-Hao Liu,Xin-Yu Zhao,Shi-Sheng He,Feng Chen,Anatoly I. Kulak,Bing-Qiang Lu
Diabetes-associated chronic wound management has been a serious worldwide issue, which is obstructed by multiple factors such as sustained inflammation, attenuated angiogenesis, and fragile defense against bacterial infection in the wound niche. Thus, there stands an urgent demand for biomaterial dressings that are able to treat such complex conditions. Herein, we developed a composite biomaterial of gallic acid-modified bioglass ( [email protected] ) in an anhydrous system, by using two agents which have already been used in clinical practice. The prepared [email protected] not merely promoted the migration and angiogenesis of vascular endothelial cells but also provided superior antibacterial performance in the local environment. Significantly, the gallic acid-assisted modification made the color of bioglass (BG) change from white to black, endowing it with high light absorption to successfully achieve a significant photothermal property. Then, the strengthened antibacterial effects were further realized by irradiation with near-infrared light (NIR) based on this photothermal property. Particularly, the combined therapy ( [email protected] + NIR) was demonstrated as an effective strategy in treating the infected full-thickness dermal wounds of diabetic rats. This research provides a convenient non-antibiotic medicine formula for diabetic wounds with infection, and demonstrates a valid modification for inorganic bioglass-based material with organic polyphenol, which inspires more expansive composites for wound biomaterials.
研究领域:
材料科学
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生物材料
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伤口愈合
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糖尿病
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光热治疗
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2010
·Vol. 108
·期 1
·175-185
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IF 3.377
Jiankang He,Yanan Du,Yuqi Guo,Matthew J. Hancock,Ben Wang,Hyeongho Shin,Jinhui Wu,Dichen Li,Ali Khademhosseini
Combinatorial material synthesis is a powerful approach for creating composite material libraries for the high-throughput screening of cell–material interactions. Although current combinatorial screening platforms have been tremendously successful in identifying target (termed “hit”) materials from composite material libraries, new material synthesis approaches are needed to further optimize the concentrations and blending ratios of the component materials. Here we employed a microfluidic platform to rapidly synthesize composite materials containing cross-gradients of gelatin and chitosan for investigating cell–biomaterial interactions. The microfluidic synthesis of the cross-gradient was optimized experimentally and theoretically to produce quantitatively controllable variations in the concentrations and blending ratios of the two components. The anisotropic chemical compositions of the gelatin/chitosan cross-gradients were characterized by Fourier transform infrared spectrometry and X-ray photoelectron spectrometry. The three-dimensional (3D) porous gelatin/chitosan cross-gradient materials were shown to regulate the cellular morphology and proliferation of smooth muscle cells (SMCs) in a gradient-dependent manner. We envision that our microfluidic cross-gradient platform may accelerate the material development processes involved in a wide range of biomedical applications. Biotechnol. Bioeng. 2011; 108:175–185. © 2010 Wiley Periodicals, Inc.
研究领域:
生物工程
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材料科学
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细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）