CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2015
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IF 0.754
Liang-Liang HUANG,Yan HUANG,Yan-Kai CHEN,Yun-Hua DING,Wei-Feng ZHANG,Xiao-Jing LI,Xiao-Ping WU
A simple and green method for the determination of trace bisphenol A (BPA) was established by coupling supported ionic liquid solid-phase extraction to β -cyclodextrin modified ionic liquids carbon paste electrode-based electrochemical detection (SILs-SPE-ED). The synthesized imidazolium ionic liquids modified styrene type macroporous resin was used as adsorbent for SPE of BPA. The experimental parameters that affected the extraction efficiency were investigated and optimized. Dynamic adsorption test showed that the maximum adsorption capacity of BPA on the SILs-SPE cartridge was 10.1 mg g −1 , and the enrichment factor was 40. The calibration curve showed a good linearity between the anodic current and the BPA concentration in the range of 1.0 × 10 −8 −1.0 × 10 −6 M. The detection limit was 4.16 nM (equal to 0.95 μg L −1 ). The SILs-SPE-ED method, with the advantages including high sensitivity and good practicability, was applied to the analysis of real water and plastic samples, and the results agreed well with HPLC method.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Kang Lu,Zuxian Liu,Yi Shen
Monitoring glucose concentration in blood is an effective method to assess health status, and value-added glucose conversion can contribute to sustainable development. This study reports the utilization of platinum-based alloys consisting of Pt, PtRh, and PtRhNi nanoparticles supported by graphene nanosheet (GNS) as bifunctional catalysts for the electrochemical detection and conversion of glucose. Electrochemical results showed that the PtRhNi/GNS exhibited a peak current density of 0.044 mA cm −2 , which was two and four times higher than that of Pt/GNS and PtRh/GNS, respectively. The electrochemical sensor prepared by PtRhNi/GNS demonstrated a linear detection range of 0.06–0.36 mM for glucose with a minimum detection limit of 18.9 μM, and exhibits good stability, reproducibility, and immunity to interference. PtRhNi/GNS exhibited the best catalytic activity for glucose, with 86.4 % conversion of glucose at 0.666 V vs. RHE. Glyoxalate was the dominant product with a selectivity of 93 %. Moreover, the product distribution could be enhanced by optimizing the applied potential and electrolysis time. This study provides a promising method for the effective detection and utilization of glucose.
研究领域:
生物化学
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材料科学
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医学诊断
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Junpeng Lv,Di Wu,Yingming Ma,Xiyao Zhang,Wenhui Xu,Mengge Wang,Songmin Chen,Qiong Hu,Dongxue Han,Li Niu
Glycoproteins have become the most often screened tumor markers in the in vitro diagnostics. Although a large number of electrochemical methods have been proposed to sensitively detect glycoproteins, most of them involve the aid of laborious signal amplification. Herein, we report the use of glycan-evocated metallization (GlyMetal) for the amplification-free electrochemical detection of glycoproteins at low concentration levels. Briefly, the glycoproteins of interest are captured by an aptamer recognition layer, and then the glycans of targets are oxidized by NaIO4 to convert the 1,2-diol sites into aldehyde groups for the silver deposition-based metallization, followed by the electrochemical stripping assay of the deposited metallic silver for glycoprotein quantification via the established solid-state Ag/AgCl voltammetric process. As GlyMetal can enable the deposition of a large amount of metallic silver and a high signal-to-background ratio can be obtained for the solid-state Ag/AgCl voltammetric stripping assay, the developed GlyMetal-based electrochemical method is applicable to the amplification-free detection of glycoproteins. As a proof of concept, a detection limit of 1.65 pg/mL has been achieved for carcinoembryonic antigen (CEA) detection. In addition to the high selectivity, desirable results have been obtained with respect to the use of the method for CEA detection in serum samples. In consideration of the desirable simplicity, short assay time, and cost-effectiveness of the amplification-free approach, the GlyMetal-based electrochemical method shows great promise in the point-of-care detection of glycoproteins.
研究领域:
生物化学
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医学诊断
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分析化学
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产品关联
DOI
PubMed
高影响因子
International Journal of Analytical Chemistry
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2024
·
IF 1.5
Bingyuan Fan,Qian Wang,Shan Wang,Yahui Gao,Yan Liang,Jinru Pan,Xinrui Fu,Li Li,Wei Meng
MiR-378 is abnormally expressed in various cancers, such as hepatocellular carcinoma, renal cell carcinoma, and nonsmall cell lung cancer. Here, we developed a label- and immobilization-free ratiometric homogeneous electrochemical strategy based on exonuclease III (Exo III) for the facile and rapid determination of miR-378. Two 3′-protruding hairpin DNA probes (HPs) are designed in this strategy. Doxorubicin (DOX) and potassium ferrocyanide (Fe 2+ ) were used as label-free probes to produce a response signal (I DOX ) and a reference signal (I Fe 2+ ) in the solution phase. When no target was present in the solution, the HP was stable, most of the DOX was intercalated in the stem of the HP, and the diffusion rate of DOX was significantly reduced, resulting in reduced electrochemical signal response. When miR-378 was present, double-cycle signal amplification triggered by Exo III cleavage was initiated, ultimately disrupting the hairpin structures of HP1 and HP2 and releasing a large amount of DOX into the solution, yielding a stronger electrochemical signal, which was low to 50 pM. This detection possesses excellent selectivity, demonstrating high application potential in biological systems, and offers simple and low-cost electrochemical detection for miR-378.
研究领域:
生物化学
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分子生物学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Science Advances
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2024
·Vol. 10
·期 49
·
IF 11.7
Chaoyue Gu,Fanzhen Kong,Sen Liang,Xiang Zhao,Bingjie Kong,Tianhe Jiang,Jianan Yu,Qi Li,Yuqing Lin,Shuo Bai,Yuanhua Shao
Chloride ion, a pivotal cerebral anion involved in neuronal inhibition, is implicated in various neurodegenerative diseases. Conventional direct faradaic detection based on electron transfers at solid electrode/solution interfaces has been proven ineffective due to the electrochemically inactive nature of Cl−. Here, we present an approach involving molecularly tailored liquid/liquid interfacial ultramicro iontronics (L/L-UIs) supported at ultramicropipettes filled with organic gel containing lipophilic bis-thioureas ionophores, which represents the first application of amperometric methodology based on electrochemical facilitated ion transfers reactions at a soft L/L ultramicrointerface to achieve in vivo sensing of electrochemically inactive ions, and dynamically tracking cerebral Cl− in vivo. Furthermore, evidence of dynamic neuronal Cl− regulation via KCC2 modulated through GABAB receptors was provided, further substantiating GABAB receptor–mediated Cl−-related neuronal inhibition. The proposed L/L-UIs have notable potential for in situ tracking of other crucial electrochemically inactive ions or ionized biomolecules in vivo, thereby facilitating the study of brain diseases and the diagnosis and treatment of related disorders.
研究领域:
神经科学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2025
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IF 6.1
Pengshen Jing,Qian Chen,Haifeng Ke,WenHui Xu,Yanhong Ni,Chun Mao,Runzhi Deng
In the fields of neuroscience and medicine, the accurate capture and quantitative analysis of dopamine (DA) levels in the blood are of great clinical significance for the early diagnosis of neurological dysfunctions and other pathological conditions. In this study, we developed a laccase (La) sensor based on micromotor technology to accurately detect DA content in blood. Firstly, the magnesium-based micromotor was used to endow the sensor with autonomous motion performance, which significantly increased the contact frequency between La and DA molecules in blood. Secondly, the micromotor-loaded Fe 3 O 4 /PPY/La magnetic nanoparticles provide an efficient electrical signal transmission interface for La, while the presence of polypyrrole (PPY) prevented electrode passivation caused by La. In addition, we used magnetic to collect Fe 3 O 4 /PPY/La after capture and tested it by differential pulse voltammetry. Finally, thanks to the excellent catalytic performance and high selectivity of La, the effective recognition and detection of DA in blood samples were realized, and an efficient electrochemical detection method was developed. This innovative detection scheme has a wide linear detection range and high sensitivity, which is expected to provide a more accurate diagnostic tool for health problems related to DA abnormalities, so as to better cope with and prevent related diseases.
研究领域:
神经科学
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医学
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2019
·Vol. 9
·期 4
·513
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IF 4.034
Jian Hu,Hao Dai,Yanbo Zeng,Yiwen Yang,Hailong Wang,Xudong Zhu,Lei Li,Guobao Zhou,Ruoyu Chen,Longhua Guo
In this study, we report a cross-linker-based poly(ionic liquid) (PIL) for the sensitive detection of 4-nonylphenol (4-NP). PIL was poly(1,4-butanediyl-3,3′-bis-l-vinylimidazolium dibromide) (poly([V2C4(mim)2]Br2)). Poly([V2C4(mim)2]Br2) was prepared via one-step free-radical polymerization. The poly([V2C4(mim)2]Br2) was characterized by infrared spectroscopy, Raman spectroscopy, thermal gravimetric analyzer and scanning electron microscope. The poly([V2C4(mim)2]Br2) was then drop-cast onto a glassy carbon electrode (GCE) to obtain poly([V2C4(mim)2]Br2)/GCE. In comparison with a bare GCE, poly([V2C4(mim)2]Br2)/GCE exhibited higher peak current responses for [Fe(CN)6]3−/4−, lower charge transfer resistance, and larger effective surface area. While comparing the peak current responses, we found the peak current response for 4-NP using poly([V2C4(mim)2]Br2)/GCE to be 3.6 times higher than a traditional cross-linker ethylene glycol dimethacrylate (EGDMA) based poly(EGDMA) modified GCE. The peak current of poly([V2C4(mim)2]Br2) sensor was linear to 4-NP concentration from 0.05 to 5 μM. The detection limit of 4-NP was obtained as 0.01 μM (S/N = 3). The new PIL based electrochemical sensor also exhibited excellent selectivity, stability, and reusability. Furthermore, the poly([V2C4(mim)2]Br2)/GCE demonstrated good 4-NP detection in environmental water samples.Keywords:poly(ionic liquid);cross-linker;electrochemical detection;4-nonylphenol
研究领域:
环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 6
·B456
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IF 3.12
Aijuan Xie,Jiawen Du,Jie Zhang,Zhichen Xiong,Fei Shao,Shiping Luo
A novel graphene-NiO-polyaniline (Gr-NiO-PANI) was prepared by electrolysis, electrodeposition and electropolymerization methods, respectively. The X-ray diffraction (XRD) proved the successful preparation and composition of the Gr-NiO-PANI hybrid. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were used to further determine the structure and morphology of as-prepared composites. Cyclic voltammetry confirmed that Gr-NiO-PANI composite had excellent detection effect on urea. By comparing the electrochemical behavior of Gr-NiO-PANI with Gr-NiO and Gr-PANI modified electrodes toward urea, the role of NiO in composite and the detection mechanism of urea were explored. A common linear sweep voltammetry technique was used to detect urea. The test results showed that urea exhibited a good response on the Gr-NiO-PANI modified electrode. In the range of 60∼160 μM urea concentration, the response current of the Gr-NiO-PANI modified electrode is proportional to the urea concentration, and the concentration stand curve equation was I(μA) = −0.0886C(μM) + 58.39 (R2 = 0.9917). The as-fabricated sensor exhibited high sensitivity (1.266 μA/μM), lower limit of detection (7.35 μM), excellent reproducibility, and so it can provide a simple and cheap method for detection of urea in environmental monitoring, agriculture, medical industry.
研究领域:
材料科学
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生物医学工程
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环境监测
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Kai Wen,Xingdong Yang,Cuiling Zhang,Huiying Wei,Guizheng Zou,Ye Zhu
Diffusion-mediated homogeneous electrochemical assay has attracted substantial attention owing to its simplicity, rapid response, and high recognition efficiency, however, lack of inherent connection between signal reporter and electrode surface restricts the improvement of detection sensitivity and further limits its potential applications. Herein, a facile electrochemical strategy is proposed through integrating the advantages of traditional heterogeneous assay and homogenous assay. In this strategy, a small amount of target DNA can trigger the release of assist DNA from the electrode surface. Then, the assist DNA can induce the homogeneous amplification reaction, which in turn promotes the amplification on the electrode surface by generating numerous target DNA analogues, thereby realizing positive feedback signal amplification. As a result, ultrasensitive electrochemical detection of nucleic acids was achieved in the dynamic range from 5 aM to 5 fM with a detection limit down to 1.2 aM. There is about 2.5 orders of magnitude improvement in sensitivity compared with the control strategy without homogeneous amplification. Moreover, the proposed strategy was demonstrated with high selectivity, reproducibility, and capability of detecting nucleic acids in serum samples, displaying its promising potential in the development of ultrasensitive electrochemical biosensing platform for disease diagnosis applications.
研究领域:
生物化学
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分析化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Shuo Wu,Yan Zhao,Xunxun Deng,Xinlan Yang,Xiuyun Wang,Yanqiu Zhao
This work demonstrates that the photoelectrochemical response of the CdS/Bi 2 O 2.33 direct Z-scheme heterojunction, synthesized by in situ deposition of CdS nanocrystals on the defect engineered Bi 2 O 2.33 , can be modulated by oxygen defect concentration. The appropriate oxygen defects not only increase the visible light absorption, provide active reaction sites to enhance PEC activity, but also promote the separation of carriers. The formation of CdS/Bi 2 O 2.33 direct Z-scheme heterojunction further improves these properties by extending the visible light absorption and promoting separation and transport of carriers, but avoids the usage of noble metal nanoparticles as electron transfer mediators, thus has a low cost and easy fabrication technology. The CdS/Bi 2 O 2.33 direct Z-scheme junction shows significantly improved photocurrent response as compared with those containing less oxygen defects, and is applied as a photoelectrochemical assay platform for Hg 2+ . The specific interaction between Hg 2+ and the S 2− in CdS significantly quenches the photocurrent response of the CdS/Bi 2 O 2.33 due to the formation of HgS. The photocurrent decrease is linear to the concentration of Hg 2+ in the range from 10 −11 to 10 −6 g/mL, with the limit of detection of 3.2 pg/mL. High accuracy and good reproducibility are realized in the real sample analysis of urine, river water, and sea water. The integration of oxygen defect engineering and direct Z-scheme electron transport principle provides a new avenue for fabricating high performance photoelectrochemical materials, which can be further combined with bio-recognition strategy for the ultrasensitive detection of biological molecules.
研究领域:
材料科学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Yingying Sheng,Yifu Zhu,María Luisa Cerón,Yufu Yi,Peng Liu,Peng Wang,Ting Xue,María Belén Camarada,Yangping Wen
Nanozyme based on the silver nanoparticles (AgNPs) decorated highly water-oxygen stable phosphorene (BP) nanohybrid with phosphorus-doped porous carbon microspheres (P-PCMs) is applied as an intelligent sensing platform for the electrochemical detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a biomarker of oxidative DNA damage in human urine sample. The density functional theory (DFT) is use for investigating the effect of silver on the conductivity level of BP and discussing a possible mechanism for the electrocatalytic oxidation of 8-OHdG. The Ag + is in-situ reduced as AgNPs that grows onto the BP surface for the controllable preparation of BP-AgNPs with effective surface passivation among different metal ions decorated BP. P-PCMs with 4.9-fold enhancement in specific surface area (1636.73 m 2 g −1 ) are prepared by hydrothermal carbonization of α-cyclodextrin as carbon sources, then calcinate in the presence of phosphoric acid as an activator and dopant. P-PCMs-BP-AgNPs are prepared by the ultrasound-assisted liquid-phase exfoliation with the addition of Ag + , and both P-PCMs and black phosphorus crystals are sufficiently grinded. The P-PCMs-BP-AgNPs nanohybrid displays good long-term water-oxygen stability, extraordinary specific surface area, superior electrocatalytic capacity with 303-fold enhancement, enzyme-like characteristics with I max of 100 μA and K m of 29 μM. The machine learning (ML) model with artificial neural network (ANN) algorithm is employed for the intelligent output of 8-OHdG in real sample with acceptable recovery in work range from 0.2 to 125 μM.
研究领域:
生物医学工程
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材料科学
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分析化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-11
·
IF 5.479
Shi Erbin,Yu Guangli,Lin Huiming,Liang Cuiyuan,Zhang Tingting,Zhang Feng,Qu Fengyu
The first example of metallic bismuth encapsulated into a mesoporous metal-organic framework of the type MIL-101(Cr) matrix is presented. Bi(III)-impregnated MIL-101(Cr) (Bi(III)/MIL-101(Cr)) was dropped onto a conductive carbon cloth electrode (CCE). Then, bismuth was generated by electrochemical reduction of the Bi(III)/MIL-101(Cr) supported on CCE (Bi/MIL-101(Cr)/CCE). The resulting Bi/MIL-101(Cr)/CCE display impressive performance in terms of peak currents for the ions Cd(II) and Pb(II) when compared to the single-component counterparts. Differential pulse anodic stripping voltammetry (DPASV) enabled sensing of the two ions over linear working range of 0.1 to 30 μg L −1 and 30 to 90 μg L −1 . The parameters are refined before the detection of two metal ions, including the amount of bismuth in MIL-101(Cr), optimum pH (5.0), deposition potential (−1.2 V) and deposition time (600 s). The respective detection limits are 60 and 70 ng L −1 (at S/ N = 3). This is strikingly lower than the guideline values of domestic water given by the WHO which are 3 μg L −1 for Cd(II) and 10 μg L −1 for Pb(II). The Bi/MIL-101(Cr) onto CCE is fairly specific for Cd(II) (at around −0.76 V) and Pb(II) (at around −0.54 V), well reproducible and has excellent recovery in real water analysis.
Schematic illustration of the preparation of a Bi(III)/MIL-101(Cr) metal-organic framework, its deposition on a carbon cloth electrode (CCE), and its application for detection of Cd(II) and Pb(II) by differential pulse adsorptive stripping voltammetry (DPASV).
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）