Advanced Science
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2024
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IF 14.3
Fugang Liu,Jiaqing Liu,Yang Luo,Siyi Wu,Xu Liu,Haoran Chen,Zhewen Luo,Haitao Yuan,Feng Shen,Fangfang Zhu,Jian Ye
Metabolic dysregulation is a key driver of cellular senescence, contributing to the progression of systemic aging. The heterogeneity of senescent cells and their metabolic shifts are complex and unexplored. A microfluidic SlipChip integrated with surface-enhanced Raman spectroscopy (SERS), termed SlipChip-SERS, is developed for single-cell metabolism analysis. This SlipChip-SERS enables compartmentalization of single cells, parallel delivery of saponin and nanoparticles to release intracellular metabolites and to realize SERS detection with simple slipping operations. Analysis of different cancer cell lines using SlipChip-SERS demonstrated its capability for sensitive and multiplexed metabolic profiling of individual cells. When applied to human primary fibroblasts of different ages, it identified 12 differential metabolites, with spermine validated as a potent inducer of cellular senescence. Prolonged exposure to spermine can induce a classic senescence phenotype, such as increased senescence-associated β-glactosidase activity, elevated expression of senescence-related genes and reduced LMNB1 levels. Additionally, the senescence-inducing capacity of spermine in HUVECs and WRL-68 cells is confirmed, and exogenous spermine treatment increased the accumulation and release of H 2 O 2 . Overall, a novel SlipChip-SERS system is developed for single-cell metabolic analysis, revealing spermine as a potential inducer of senescence across multiple cell types, which may offer new strategies for addressing ageing and ageing-related diseases.
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DOI
PubMed
高影响因子
Nature Biomedical Engineering
·
2025
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IF 26.6
Li Cuifeng,Li Yuxin,Zhou Bini,Li Tong,Wei Xiaohui,Chen Kun,Chen Wenlong,Shi Ziwei,Dai Xiaobin,Zhang Jiachen,Yang Chen,Ji Zhenglin,Sun Wenbo,Gao Ji,Wu Jing,Zhao Binbin,Min Xin,Li Yujie,Lin Lin,Yang Wei,Wang Min,Liu Zezhong,Liu Yaping,Zhu Chenyou,Yang Bo,Xu Jiang-Fei,Yan Li-Tang,Shi Yi,Lu Lu,Zhang Linqi,Ding Qiang,Xue Jing,Hou Baidong,Qi Hai,Liu Jiangning,Yang Yuhe R.,Liu Dongsheng,Liu Wanli
The emergence of novel infectious disease has intensified demand for more advanced vaccine development and more potent adjuvants to enhance immunogenicity. Here we introduce a dynamic DNA supramolecular matrix assembled from five unmodified, short DNA single strands, serving as a safe, multifaceted adjuvant platform. This DNA matrix elicits a robust humoral response with minimal adverse effects, generating potent neutralizing antibodies and conferring robust protection against SARS-CoV-2 and Streptococcus pneumoniae infections. Its dynamic colloidal feature prolongs the in vivo retention of both DNA and antigen, facilitating lymphatic-targeted transportation and presentation. This process leads to a robust pro-inflammatory response in both the vaccinated site and draining lymph node, which, in turn, promotes the recruitment and activation of immune cells, leading to a rapid, effective antigen-specific antibody response. The enhanced function of DNA matrix depends on the canonical TLR9–MyD88 signalling axis in dendritic cells. In addition, only right-handed, not left-handed, chirality of the DNA strands forms d -DNA matrix and promotes immune activations. Thus, this DNA matrix functions as an all-in-one adjuvant platform, opening promising avenues for future vaccine design.
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PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2024
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IF 1.5
Lei Sijie,Lin Xiaoyan,Han Qiang
In this study, polyacrylonitrile was modified to polyamidoxime to enhance foam stabilization and decontamination rate. Polyamidoxime was combined with graphene oxide, 1 wt% of saponins, and 1 wt% of alkyl glycosides to formulate the PAO/GO foam decontaminant. The outcomes demonstrated resulted in the half-life of the foam extending from 32 to 308 min. The PAO/GO foam decontaminant displayed α-ray decontamination rates of 93.30%, 98.34%, and 99.81% on stainless steel, tile, and glass surfaces. Hence, the dual functional PAO/GO in stabilizing foam while enhancing decontamination rates underscores its potential for radioactive decontamination.
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高影响因子
PEST MANAGEMENT SCIENCE
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2025
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IF 3.8
Huanwen Chen,Dan Xie,Zikai Pan,Niya Jia,Junhua Zhou,Qingshan Yang,Jia Yu,Defu Chi
BACKGROUND The red-haired bark beetle (RHB), Hylurgus ligniperda (Fabricius), is a significant invasive pest responsible for widespread mortality in pine forests. Current control methods are largely ineffective and poorly integrated, failing to halt the spread of RHB. This study aimed to develop and validate an integrated management system that leverages the beetle's behavioral patterns and implements stratified management strategies based on the severity of infestation levels. RESULTS We first enhanced trapping efficiency by optimizing key technologies, including trap design, deployment methods, and lure formulations. Building on this foundation, we developed a stratified management plan with the following key measures: in newly colonized stands, a strategy combining benzyl alcohol repellents and optimized lure attractants, along with flight-interception traps, significantly reduced spread, decreasing the proportion of dead trees from 12.5% to 2.6%. In outbreak stands, intensive measures such as stump removal and mass trapping reduced the RHB population density by 99.05%. Additionally, replanting alters stand volatiles, reducing their attractiveness to the beetles. CONCLUSION The stratified management strategy, applied to both newly colonized and outbreak stands of RHB, proved highly effective in suppressing population growth and preventing further dispersal. This approach offers a key technical solution for restoring damaged coastal ecosystems and provides a valuable reference for managing similar invasive pests worldwide. © 2025 Society of Chemical Industry.
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高影响因子
Biomaterials Science
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2025
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IF 5.7
Jingsong Lu,Baoli Ma,Ying Li,Sumei Chen,Muyan Zhang,Zhenhu Guo,Xiaohan Gao,Neema Ufurahi Pambe,Abdul Fahad,Wensheng Xie,Xiaodan Sun,Xiumei Wang,Guifeng Zhang,Jing Yu,Shenglei Che,Yen Wei,Lingyun Zhao
Pore formation can facilitate the release of various intracellular substances upon cell death, which is of critical benefit for tumor immunotherapy by immunogenic cell death (ICD). Given the effective endogenous antigen release is of primary importance for ICD-eliciting immunogenicity, other than by enhancing the cytotoxicity of ICD, we designed an immunogenic induction strategy via pore formation based on saponin, a composite medium of the membrane-disrupting agent. In this study, saponin/polyphenol (ZS-TA) at proper concentrations can directly cause membrane perforation by removal of cholesterol from the membrane, thereby lead to the release of intracellular substances. Simultaneously, in situ nano-antigens (nano-Ag) would be formed through the mechanism of protein-polyphenol interaction and the nano-Ag would be served as reservoirs of antigen to trigger long-term immune effects. Meanwhile, this membrane perforation could also enhance the uptake of chemotherapeutic drugs, which could be served as a general approach for drug delivery. Therefore, this work insights for the design of enhanced drug delivery system and in situ vaccines to sensitize tumor chemo-immunotherapy.
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高影响因子
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