ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Fang Yang,Xiaoxian Zhang,Lina Song,Huating Cui,John N. Myers,Tingting Bai,Ying Zhou,Zhan Chen,Ning Gu
Uniform and multifunctional poly(lactic acid) (PLA)–nanoparticle composite has enormous potential for applications in biomedical and materials science. A detailed understanding of the surface and interface chemistry of these composites is essential to design such materials with optimized function. Herein, we designed and investigated a simple PLA–magnetic nanoparticle composite system to elucidate the impact of nanoparticles on the degradation of polymer–nanoparticle composites. In order to have an in-depth understanding of the mechanisms of hydrolysis in PLA–nanoparticle composites, degradation processes were monitored by several surface sensitive techniques, including scanning electron microscopy, contact angle goniometry, atomic force microscopy, and sum frequency generation spectroscopy. As a second-order nonlinear optical technique, SFG spectroscopy was introduced to directly probe in situ chemical nature at the PLA–magnetic nanoparticle composite/aqueous interface, which allowed for the delineation of molecular mechanisms of various hydrolysis processes for degradation at the molecular level. The best PLA–NP material, with a concentration of 20% MNP in the composite, was found to enhance the drug release rate greater than 200 times while maintaining excellent controlled drug release characteristics. It was also found that during hydrolysis, various crystalline-like PLA domains on the surfaces of PLA–nanoparticle composites influenced various hydrolysis behaviors of PLA. Results from this study provide new insight into the design of nanomaterials with controlled degradation and drug release properties, and the underlined molecular mechanisms. The methodology developed in this study to characterize the polymer–nanoparticle composites is general and widely applicable.
研究领域:
生物医学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Ting-Ting Xia,Chun-Zhao Liu,Jian-Hua Hu,Chen Guo
Polyethylenimine (PEI) as a spacer-arm polymer was modified on amine-functioned Fe 3 O 4 nanoparticles (Fe 3 O 4 –NH 2 NPs). PEI modified Fe 3 O 4 nanoparticles (Fe 3 O 4 –NH 2 –PEI NPs) were chelated with Cu 2+ to immobilize laccase through metal affinity adsorption. Meanwhile, PEI un-modified Fe 3 O 4 –NH 2 NPs acted as a control group. The adsorption capacity of Fe 3 O 4 –NH 2 –PEI–Cu 2+ NPs was larger than Fe 3 O 4 –NH 2 –Cu 2+ NPs almost in the whole range of laccase concentration. And the activity recovery of Fe 3 O 4 –NH 2 –PEI–Laccase was also higher than Fe 3 O 4 –NH 2 –Laccase when the laccase concentration was lower than 26 μg/mL. The maximum activity recovery of Fe 3 O 4 –NH 2 –PEI–Laccase (107.41%) was much higher than Fe 3 O 4 –NH 2 –Laccase (42.75%). The corresponding specific activity of Fe 3 O 4 –NH 2 –PEI–Laccase and Fe 3 O 4 –NH 2 –Laccase was separately 101.33 and 74.45 times as large as free laccase product. The enzymatic properties of the two immobilized laccases were both improved and the immobilization realized reuse of laccase. The results showed that Fe 3 O 4 –NH 2 –PEI–Cu 2+ NPs were more promising than Fe 3 O 4 –NH 2 –Cu 2+ NPs for purification and immobilization of laccase simultaneously and are potential for large-scale laccase immobilization.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Xinqi Xu,Tianheng Chen,Lian Xu,Juan Lin
Laccase is an efficient biocatalyst for oxidative polymerization of organic substrates. However, cost of enzyme preparation , low stability and residual protein diminish the efficiency of laccase mediated polymerization. In this work, a series of silicon dioxide coated ferroferric oxide magnetic nanoparticles were modified by different functional groups including γ-methacryloxypropyltrimethoxy, succinic anhydride, glutaraldehyde and polyethylene imine. Infrared spectra indicated the magnetic carriers have been successfully modified. Vibrating sample magnetometer (VSM) analysis revealed that all of these carriers showed high magnetic responsiveness after the surface functionalization. Laccase from Cerrena sp. HYB07 was then respectively immobilized covalently on these functionalized magnetic carriers. All the immobilized laccases displayed higher thermostability than free laccase and glutaraldehyde functionalized support (named FSNG) immobilized laccase showed better performance. These immobilized laccases all showed higher efficiency than free laccase for oxidative polymerization of catechol and hydroquinone. The immobilized laccases could be separated from the water insoluble polymerization products. The polymerization product of hydroquinone by FSNG immobilized laccase showed the average polymerization degree of the poly(hydroquinone) was six (DP=6). This work provided a comprehensive exploration of laccase immobilization on magnetic carrier for catalyzing polymerization of phenols .
研究领域:
生物化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Progress & Sustainable Energy
·
2024
·
IF 2.1
Wenjun Zhou,Zhixiang Lv,Yao Wang,Sheng Luo,Dan Zhou,Guodong Su
Magnetic Cu 0.2 Zn 0.3 Co 0.5 Fe 2 O 4 nanoparticles were prepared by the rapid combustion method and characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), x-ray diffraction (XRD), and vibrating sample magnetometer (VSM). The average particle size and the saturation magnetization of the nanoparticles prepared at 400°C with 25 mL absolute alcohol were about 60.9 nm and 50 emu/g. The results of the experiment displayed that the adsorption process agreed with the pseudo-second-order kinetics model ( R 2 > 0.98) and Langmuir isotherm model ( R 2 = 0.9982), indicating that the adsorption of DB-2BLN onto magnetic Cu 0.2 Zn 0.3 Co 0.5 Fe 2 O 4 nanoparticles was monolayer chemisorption. Δ H (Δ H = −28.0135 kJ/mol) of the thermodynamic experiment was less than 0, indicating that the adsorption was an exothermic process. The effects of pH, initial concentration of dye, ionic strength, temperature, and adsorbent dosage on the adsorption process of DB-2BLN onto magnetic Cu 0.2 Zn 0.3 Co 0.5 Fe 2 O 4 nanoparticles and the regeneration performance of the nanoparticles were investigated. When the pH was determined to be 2 and the adsorbent dosage was 5 mg, the adsorption capacity reached the maximum. After 7 cycles, the removal rate of DB-2BLN still reached 92.6% of that for the first adsorption, showing excellent regeneration performance. Finally, the electrochemical properties of the magnetic Cu 0.2 Zn 0.3 Co 0.5 Fe 2 O 4 nanoparticles were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
研究领域:
材料科学
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环境科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
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2017
·Vol. 7
·期 66
·41919-41928
·
IF 3.108
Chaoping Fu,Rui-Meng Yang,Li Wang,Nan-nan Li,Meng Qi,Xiang-dong Xu,Xin-hua Wei,Xin-Qing Jiang,Li-Ming Zhang
For combinatorial monitoring and chemotherapy of hepatocellular carcinoma, the macromolecular prodrug based on hyaluronic acid and doxorubicin hydrochloride was prepared by an acid-labile hydrazone linkage, and then conjugated with amine-modified iron oxide nanoparticles by a carbodiimide mediated coupling reaction. The resultant hybrid nanoparticles have the characteristics of good water dispersibility, superparamagnetic property and high magnetic relaxivity for magnetic resonance imaging. In particular, they demonstrated significant cellular uptake and specific accumulation in human hepatocellular liver carcinoma HepG2 cells due to specific biological recognition of hyaluronic acid. In addition, in vitro cumulative release of doxorubicin hydrochloride from the resultant hybrid nanoparticles was significantly accelerated at a mildly acidic pH of 5.0–6.0 when compared to its release at the physiological pH of 7.4, suggesting the pH-responsive feature of this conjugated prodrug for effective chemotherapy of hepatocellular carcinoma. In vivo antitumor efficacy of the mice further confirmed the effect.
研究领域:
生物医学工程
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肿瘤学
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药剂学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Shu Yan,Zhiyuan Yuan,Hehe Qian,Yitong Dai,Bin Sun,Pengfei Jiang,Yongsheng Guo,Wenjun Fang
Environmental contamination from oil spills and industrial oily wastewater poses significant ecological risks due to the persistence of harmful organic compounds. To address these challenges, magnetic composite nanospheres (CMNP@CHPEI) are systematically developed, with carboxylated Fe 3 O 4 nanoparticles (CMNP) as the core and amphiphilic hyperbranched polyethyleneimine (CHPEI) as the decorated shell. These novel nanospheres combine the controllable size and magnetic responsiveness of “hard” magnetic nanomaterials with the structural complexity and functional diversity of “soft” hyperbranched polymers. This design allows for switching between emulsification and demulsification behaviors by regulating the size of the nanospheres and the amphiphilicity of CHPEI. Specifically, the nanospheres can form Pickering emulsions with oil droplet sizes smaller than 1 µm, maintaining stability for up to 75 days, and achieve rapid oil-water separation with demulsification efficiencies up to 99.8 %. Even after seven recycling experiments, they still retain significant interfacial activity and applicability. Interfacial characteristic experiments and molecular dynamics simulations reveal that particle size directly affects the film structures formed at oil-water interface, while the amphiphilic functional molecules determine the interaction mode of nanospheres with oil-water phases. These achievements introduce a versatile, environmentally friendly material for removing hazardous oil-based pollutants, with promising applications in oil spill remediation and industrial wastewater treatment.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Liping Dong,Shun Feng,Shanshan Li,Peipei Song,Jide Wang
In this work, a soft and nondestructive approach was developed to prepare concanavalin A-chelating magnetic nanoparticles (Con A-MNPs) for selective enrichment of glycoproteins. Ethylenediamine tetraacetic acid-modified-MNPs (EDTA-MNPs) were prepared by a one-pot chemical coprecipitation method first, and then, Cu(II) cations were used as bridge groups to immobilize Con A on EDTA-MNPs. The as-prepared absorbents with a mean diameter of 15 nm showed a strong magnetic response to an externally applied magnetic field. The results of thermogravimetric analysis showed the content of immobilized Con A was up to 28 wt %. For glycoprotein ovalbumin, the maximum capacity and equilibrium constant were 72.41 mg/g and 0.6035 L/mg, respectively. The as-prepared nanocomposites exhibited a remarkable selectivity for glycoproteins and can enrich glycoproteins specifically from a mixture of glycoprotein and nonglycoprotein even at a molar ratio of 1:600. It was also successfully applied for the enrichment of glycoproteins from real egg white samples. We expect that our finding will serve as a helpful template for others to design new adsorbents for enriching glycoproteins.
研究领域:
生物化学
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纳米技术
·
蛋白质组学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Haijiao Jia,Xiao Zhang,Guirong Borjihan,Shengbo Yang,Youxin Li
Using ruthenium chloride as catalyst precursor, magnetic silicon spheres as support, 4-formylphenylboronic acid and ethylenediaminepentaacetic acid as functional ligands, a magnetic heterogeneous nano-ruthenium catalyst (Fe 3 O 4 @SiO 2 -FPBA-DTPA-Ru) was prepared for the first time. It was nano level with 20.17 emu/g saturation magnetic strength and thermal stability under 249.63 °C. Fe 3 O 4 @SiO 2 -FPBA-DTPA-Ru contained 0.38 mmol/g Ru and was successfully used to catalyze the oxidation reactions of 15 aromatic secondary alcohols. Results proved that Fe 3 O 4 @SiO 2 -FPBA-DTPA-Ru as catalyst had good catalytic activity for the oxidation of secondary aromatic alcohols with yields exceeding 90 % for 12 products and 95 % for 10 products. High TON and TOF values (6386 and 9158 h −1 ) in 1-phenylethanol oxidation were obtained. All the reactions of aromatic alcohols were carried out in a solvent-free system, so the prepared catalyst had the characteristics of green and pollution-free. It was’nt suitable for oxidation of aromatic primary alcohols. If there was steric hindrance around the hydroxyl group in substrate, the catalysis also may be limited. The Fe 3 O 4 @SiO 2 -FPBA-DTPA-Ru catalyst also exhibited the advantages of energy saving, satisfactory reusability and stability in 9 consecutive runs. It showed great potential for future large-scale industrial production.
研究领域:
化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Shufang Yu,Guolin Wu,Xin Gu,Jingjing Wang,Yinong Wang,Hui Gao,Jianbiao Ma
A dually responsive nanocarrier with multilayer core–shell architecture was prepared based on Fe 3 O 4 @SiO 2 nanoparticles coated with mPEG-poly( l -Asparagine). Imidazole groups (pK a ∼ 6.0) were tethered to the side chains of poly( l -Asparagine) segments by aminolysis. These nanoparticles were expected to be sensitive to both magnetic field and pH environment. The obtained materials were characterized with FTIR, dynamic light scattering, ζ -potential, TEM, TGA and hysteresis loop analysis. It was found that this Fe 3 O 4 @SiO 2 –polymer complex can form nano-scale core–shell–corona trilayer particles (∼250 nm) in aqueous solution. The Fe 3 O 4 @SiO 2 , poly( l -Asparagine) and mPEG segments serve as a super-paramagnetic core, a pH-sensitive shell, and a hydrophilic corona, respectively. An antitumor agent, doxorubicin (DOX), was successfully loaded into the nanocarrier via combined actions of hydrophobic interaction and hydrogen bonding. The drug release profiles displayed a pH-dependent behavior. DOX release rate increased significantly as the ambient pH dropped from the physiological pH (7.4) to acidic (5.5). This is most likely due to protonation and a change in hydrophilicity of the imidazole groups in the poly( l -Asparagine) segments. This new approach may serve as a promising platform to formulate magnetic targeted drug delivery systems.
研究领域:
生物医学工程
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材料科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Guangbin Ji,Fei Yang,Yanzhao Yang,Jingjing Wei,Zhijie Yang
Hypothesis The ability to control the assembly of micro/nanosized particles at liquid-liquid interface with external inputs promises new opportunities in nanofabrication and biomedicines. This work aims to demonstrate a way to control of dynamic assembly of nanoparticles at liquid-liquid interface by light and magnetic field, which consequently enables the formation of dynamic emulsion droplets. Experiments Magnetic Fe 3 O 4 nanoparticles functionalized with azobenzene moieties (Fe 3 O 4 @AZO) were synthesized and were dispersed in toluene/(N, N -dimethylformamide, DMF) binary solvent. After irradiation with UV or visible light , the assembly behavior of these Fe 3 O 4 nanoparticles were evaluated by electron microscopy and fluorescent microscopy. Findings Under UV light , Fe 3 O 4 @AZO nanoparticles were self-assembled due to the increase of dipolar interaction from the photoisomerization of azobenzene and polar molecules , DMF, were harvested from a binary solvent of DMF/toluene. While under visible light, a relief of dipolar interactions between Fe 3 O 4 @AZO nanoparticles can induce the secondary assembly of these Fe 3 O 4 @AZO nanoparticles at DMF-toluene interface, resulting in DMF droplets covered by a layer of nanoparticle superlattices . More importantly, coupled with a magnetic field, these emulsion droplets can be shaped into one dimensional ones during the interfacial assembly process, thereby giving rise to dynamic emulsions controlled by light and magnetic field.
研究领域:
材料科学
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纳米技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 35
·4520-4527
·
IF 2.378
Qi Zhao,Jian Li,Yang Xu,Dandan Lv,Kanyasiri Rakariyatham,Dayong Zhou
In this study, magnetic amino-modified silica nanospheres (Fe3O4@SiO2@NH2) were prepared via coating 3-aminopropyltriethoxysilane on the surface of Fe3O4 magnetite and used for the extraction of free fatty acids (FFAs) from edible oil samples for the first time. Fourier transform infrared spectroscopy and vibrating sample magnetometer analysis indicated that Fe3O4@SiO2@NH2 was successfully synthesized and exhibited superparamagnetism. The static and dynamic adsorption experiments showed that Fe3O4@SiO2@NH2 exhibited rapid adsorption equilibrium and satisfactory adsorption capacity towards FFAs. Under optimized adsorption and analytical conditions, Fe3O4@SiO2@NH2 could effectively extract FFAs from edible oil samples with excellent linearities (0.2–1000 nmol g−1), extremely low limits of detection (0.09–0.46 nmol g−1), and satisfactory recoveries (77.4–101.8%) and precisions (3.1–9.6%). Moreover, compared to classic solid phase extraction using a commercial cartridge, the current magnetic solid phase microextraction method has many advantages including easier preparation of adsorbents, a simpler sample handling procedure and lower cost. Therefore, this method provides a promising alternative to the traditional method for the analysis of FFAs to ensure the sanitation and quality of oils.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2014
·Vol. 7
·期 7
·3100-3108
·
IF 6.739
Yajing Chen,Zhichao Xiong,Lingyi Zhang,Jiaying Zhao,Quanqing Zhang,Li Peng,Weibing Zhang,Mingliang Ye,Hanfa Zou
Highly selective and efficient capture of glycosylated proteins and peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems. Recently, hydrophilic interaction liquid chromatography (HILIC)-based functional materials have been extensively utilized for glycopeptide enrichment. However, the low amount of immobilized hydrophilic groups on the affinity material has limited its specificity, detection sensitivity and binding capacity in the capture of glycopeptides. Herein, a novel affinity material was synthesized to improve the binding capacity and detection sensitivity for glycopeptides by coating a poly(2-(methacryloyloxy)ethyl)-dimethyl-(3-sulfopropyl) ammonium hydroxide (PMSA) shell onto Fe3O4@SiO2 nanoparticles, taking advantage of reflux-precipitation polymerization for the first time (denoted as Fe3O4@SiO2@PMSA). The thick polymer shell endows the nanoparticles with excellent hydrophilic property and several functional groups on the polymer chains. The resulting Fe3O4@SiO2@PMSA demonstrated an outstanding ability for glycopeptide enrichment with high selectivity, extremely high detection sensitivity (0.1 fmol), large binding capacity (100 mg g−1), high enrichment recovery (above 73.6%) and rapid magnetic separation. Furthermore, in the analysis of real complicated biological samples, 905 unique N-glycosylation sites from 458 N-glycosylated proteins were reliably identified in three replicate analyses of a 65 μg protein sample extracted from mouse liver, showing the great potential of Fe3O4@SiO2@PMSA in the detection and identification of low-abundance N-linked glycopeptides in biological samples.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）