CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Zheng Wang,Yue Zhang,Xinyu Qu,Feng Chen,Xinyu Zhao
The rapid development of antibiotic resistance poses a huge threat to human health. Copper-based materials is a promising alternative to the traditional antibiotics due to its high efficacy and low resistance potential for bacterial infections. In this work, three-dimensional flower-like copper hydrogen phosphate nanosheet assembled microspheres (CP-NSMSs) are synthesized through a hydrolysis-assisted synthetic method using fructose-1,6-diphosphate (FDP) as the organic phosphate source. The copper hydrogen phosphate nanosheets with high aspect ratio provide a large amount of sharp edges which could penetrate bacterial membranes, resulting in the death of bacteria. Furthermore, the as-prepared CP-NSMSs exhibit the high peroxidase-like activity producing a large amount of reactive oxygen species (ROS) to kill bacteria. Based on the in vitro bacterial growth experiments, our CP-NSMSs show an excellent sterilization effect against both Gram-positive and Gram-negative bacteria. Therefore, the CP-NSMSs synthesized using the FDP as an organic phosphate source is expected to be a promising antibacterial agent with minimum resistance potential.
研究领域:
材料科学
·
生物学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Lulu Kong,DeZhu Chen,Xiaolong Zhang,Lin Zhou,Yongming Deng,Shaohua Wei
The growth of oxides on the surface of noble metals and the introduction of alloys are effective methods to enhance the catalytic activity of noble metals. However, the fabrication of noble metal oxides always involves harsh experimental conditions and the oxidation degree could not be controlled. Herein, a simple, fast, and controlled Pd oxidation method by mixing Hg2+ and Pd nanosheets (PdNSs) under mild conditions to form PdxHgy(PdO)z (PHOs) was reported, which consists of the Pd–Hg alloy and PdO. The density functional theory (DFT) calculation and experiment results proved that the Hg atom incorporation promotes the nearby Pd–Pd bond breaking, initiating a cascade of PdO generation. More precisely, 1 Hg atom incorporation induced ∼8 Pd atom oxidation, ensuring that the oxidation degree of Pd could be easily controlled by adjusting the ratio of Hg2+ and PdNS. As a peroxidase-mimicking nanozyme, the PHO affinity for its substrate could be 21.7- and 123.6-fold greater than that of the PdNS and the natural peroxidase (HRP), respectively. For the colorimetric immunoassay of carcinoembryonic antigen (CEA) detection, PHO has an ultralow detection limit of 1.33 pg mL–1, which is ∼8.6 and ∼282 times lower than that of the PdNS and the natural HRP, respectively.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 13
·5075
·
IF 4.6
Ziling Li,Tiantian Lei,Ting Pei,Keyan Chen,Zhidong Zhao,Manman Wang,Yu He
A one-step cobaltous chloride (CoCl2) molten salt method was employed to prepare multilayer MXene-Ti3C2/Co materials with further ultrasonic treatment to acquire single-layer MXene-Ti3C2/Co nanosheets (NSs). MXene-Ti3C2/Co NSs were characterized, and their enzyme-like activities were investigated. Under the catalysis of MXene-Ti3C2/Co NSs, 3,3′,5,5′-tetramethylbenzidine (TMB) could be oxidized by H2O2, with the color changing from colorless to blue. The affinity of MXene-Ti3C2/Co NSs to H2O2and TMB was better than that of nanozymes reported in previous studies. The MXene-Ti3C2/Co NSs were used for the colorimetric determination of H2O2/glucose, with limits of detection (LODs) of 0.033 mM and 1.7 μM, respectively. MXene-Ti3C2/Co NSs embedded in sodium alginate (SA) hydrogel were used to construct a sensor platform. The digital pictures combined with a smartphone-installed app (color recognizer) could be used to analyze RGB values for colorimetric detection of glucose in beverages. This point-of-care testing platform has the advantages of cost-effectiveness and good transferability, with the potential to realize quick, intelligent and on-site detection.Keywords:MXene-Ti3C2/Co NSs;peroxidase-like activity;glucose;hydrogel;point-of-care testing
研究领域:
材料科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·
IF 2.9
Jianshuai Mu,Mengjiao Ren,Ning Li,Tengyi Zhao,Zhongyi Liu,Jingwen Ma,Shulai Lei,Jiajun Wang,En-Cui Yang,Yan Wang
In recent years, great progress has been made on nanozymes with enzyme-like properties. Here, bimetallic Fe and Ni nanoclusters were anchored on the nanosheets of nitrogen-rich layered graphitic carbon nitride by one-step pyrolysis at high temperature (Fe/Ni-CN). The loading content of Fe and Ni on Fe/Ni-CN is as high as 8.0%, and the Fe/Ni-CN has a high specific surface area of 121.86 m²/g. The Fe/Ni-CN can effectively oxidize 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the presence of H2O2, and exhibits efficient peroxidase-like activity, leading to a 17.2-fold increase compared to pure graphitic carbon nitride (CN). Similar to the natural horseradish peroxidase (HRP), the Fe/Ni-CN nanozyme follows the catalytic kinetic.The Michaelis-Menten constant (Km) value of Fe/Ni-CN nanozyme for TMB is about 8.3-fold lower than that of HRP, which means that Fe/Ni-CN nanozyme has better affinity for TMB. In addition, the catalytic mechanism was investigated by combination of free radical quenching experiments and density-functional theory calculations (DFT). The results show that the high peroxidase-like activity is due to the easy adsorption of H2O2 after bimetal loading, which is conducive to the production of hydroxyl radical. Based on the extraordinary peroxidase-like activity, the colorimetric detection of p-phenylenediamine (PPD) was constructed with a wide linear range of 0.2 - 30 μM and a low detection limit of 0.02 μM. The sensor system has been successfully applied to the detection of residual PPD in real dyed hair samples. The results show that the colorimetric method is sensitive, highly selective and accurate. This study provides a new idea for the efficient enhancement of nanozyme activity and effective detection of PPD by bimetallic synergistic strategy.
研究领域:
纳米酶研究
·
生物催化
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Dai Lu,Xing Tian,Zixuan Fu,Sizhao Yang,Yixing Qiu,Yupei Yang,Bin Li,Wei Wang
Despite extensive research into nanozymes, developing highly active catalysts with broad application potential remains a significant challenge. In this study, we synthesized hollow FeNi Prussian blue analog (H-FeNi PBA) nanocages through a straightforward chemical etching method, using FeNi PBA nanocubes as precursors. The resulting H-FeNi PBA nanocages exhibited enhanced peroxidase-mimetic activity, attributed to their increased specific surface area, pore volume, and Fe 2+ content. Leveraging this activity, we developed a novel colorimetric sensing platform for the detection of mercury ions (Hg 2+ ) and L-cysteine (Cys). The platform operates based on the peroxidase-mimetic activity of H-FeNi PBA nanocages, the inhibitory effect of Cys on this activity, and the restoration of activity through the specific complexation between Cys and Hg 2+ . After optimization, the platform demonstrated wide linear detection ranges (0.1–42 μM for Hg 2+ and 1–64 μM for Cys) and low detection limits (0.035 μM for Hg 2+ and 0.24 μM for Cys). The method was successfully applied to detect Hg 2+ and Cys in real samples, showcasing its potential for environmental monitoring and biomedical applications. This work not only provides a simple strategy for synthesizing highly active nanozymes but also highlights their potential in biosensing and catalysis.
研究领域:
生物化学
·
纳米生物学
·
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
Research
·
2020
·
IF 0
Hongye Yan,Linzhe Wang,Yifeng Chen,Lei Jiao,Yu Wu,Weiqing Xu,Wenling Gu,Weiyu Song,Dan Du,Chengzhou Zhu
Carbon materials have been widely used as nanozymes in bioapplications, attributing to their intrinsic enzyme-like activities. Nitrogen (N)-doping has been explored as a promising way to improve the activity of carbon material-based nanozymes (CMNs). However, hindered by the intricate N dopants, the real active site of N-doped CMNs (N-CMNs) has been rarely investigated, which subsequently retards the further progress of high-performance N-CMNs. Here, a series of porous N-CMNs with well-controlled N dopants were synthesized, of which the intrinsic peroxidase (POD)like activity has a positive correlation with the pyridinic N content. Density functional theory calculations also reveal that pyridinic N boosts the intrinsic POD-like activity of N-CMNs. Pyridinic-N dopant can effectively promote the first H2O desorption process in comparison with the graphitic and pyrrolic N, which is the key endothermic reaction during the catalytic process. Then, utilizing the optimized nanozymes with high pyridinic N content (NP-CMNs) and superior POD-like activity, a facile total antioxidant capacity (TAC) assay was developed, holding great promise in the quality assessment of medicine tablets and antioxidant food for healthcare and healthy diet.
研究领域:
材料科学
·
生物医学工程
·
催化化学
✓ 已验证
DOI
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 8
·5730-5734
·
IF 1.483
Chen Qingai,Chen Huan,Li Zhihong,Pang Jie,Lin Tianran,Guo Liangqia,Fu FengFu
Glyphosate was found for the first time to inhibit the catalytic activity of MoS2 nanosheets, which could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine by H2O2 to produce a colorimetric reaction. In the presence of glyphosate,
the absorbance and blue color of the system was decreased. Therefore, a simple and sensitive colorimetric sensing method for glyphosate based on the peroxidase mimetic activity of MoS2 nanosheets was developed. A linear relationship between the absorbance change and concentration
of glyphosate was observed over the range of 0.4—2.0 μg/mL (R
2 = 0.993) with a detection limit (3Ω/S) of 0.087 μg/mL. The colorimetric sensing method was simple, rapid, specific and sensitive, and the detection limit of 0.087 μg/mL is lower than the maximum
contaminant level of glyphosate in drinking water set by the Environment Protection Agency of USA and World Health Organization. This method was applied to detect glyphosate in environmental water samples with satisfactory results.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Zhuo Ma,Yunfeng Qiu,Huihui Yang,Yanmin Huang,Jingjing Liu,Yue Lu,Chen Zhang,PingAn Hu
Dipeptide-polyoxometalates (POMs)-graphene oxide (GO) ternary hybrid is an excellent peroxidase-like mimic, exhibiting enhanced peroxidase-like activity compared to POMs alone. The hybrid was readily prepared through a reprecipitation method involving electrostatic encapsulation of H3PW12O40 (PW12) by cationic diphenylalanine (FF) peptide and coassembly of FF@PW12 spheres with graphene oxide (GO). Using 3,3′,5,5′-tetramethylbenzidine (TMB) as the chromogenic substrate, the peroxidase-like activity of FF@PW12 was evaluated in the heterogeneous phase, and it is 13 times higher than that of pristine PW12 in the homogeneous phase. Furthermore, ternary hybrids of FF@PW12@GO containing 5 wt % GO could enhance the activity 1.7 times higher than that of FF@PW12. The noncovalent interactions of hydrogen bonding and ionic interaction between GO and POMs are speculated to result in the synergistic effect for the enhancement of peroxidase-like performance. The strong interactions between rGO and PW12 are evaluated by a four-probe Hall measurement via the van der Pauw method, and rGO is significantly p-doped by the doping effect of PW12 with lower LUMO energy than that of the energy level of rGO and also due to the electron reservoir feature of PW12. Cyclic voltammogram measurements also suggest that GO causes significant influence on the electronic structure of the reduced forms of the redox couples of PW12. The nature of the TMB catalytic reaction may originate from the generation of the hydroxyl radical (•OH) from the decomposition of H2O2 by ternary hybrids and the formation of peroxo species of POM. Taking advantage of the UV–vis signals of TMB being correlated to the concentration of H2O2, FF@PW12@GO can be used to detect H2O2 within the limit of detection of 0.11 μM, and the detection range is 1–75 μM. The present method indeed opens up a promising route in constructing heterogeneous peroxidase-like mimics through the use of POMs via the introduction of GO for building H2O2 sensors.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Zezhou Yang,Yun Zhu,Maoqiang Chi,Ce Wang,Yen Wei,Xiaofeng Lu
The development of highly sensitive and low-cost biosensors for the detection of dopamine is of paramount importance for medical diagnostics. Herein, we report the preparation of a new peroxidase-like catalyst with a uniform heterostructure by using a technique involving electrospinning, annealing and solvothermal reaction. In this catalyst system, cobalt sulfide (CoS) nanoparticles were homogenously distributed and supported on the surface of cobalt ferrite (CoFe 2 O 4 ) nanotubes. The as-prepared CoFe 2 O 4 /CoS hybrid nanotubes showed remarkably high catalytic efficiency as peroxidase mimics toward the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Owing to the synergistic effect between the CoFe 2 O 4 and CoS component, the prepared CoFe 2 O 4 /CoS hybrid nanotubes exhibited enhanced peroxidase-like activity, exceeding that of either the CoS nanoparticles or CoFe 2 O 4 nanotubes alone. Dopamine has been widely investigated due to its unique function in the nervous system. Consequently, various approaches have been developed for the sensitive determination of dopamine. In this work, a simple and sensitive colorimetric route for the detection of dopamine was established based on the ability of dopamine to induce the reduction of oxidized TMB to TMB with consequent fading of the blue color. This method shows a wide linear range (0–50 μM) and a low detection limit of 0.58 μM. The unique heterostructure with spinel/sulfide interfaces represents a new concept for the construction of highly efficient and multifunctional biocatalysts.
研究领域:
生物传感
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Ruyi Tang,Xuemin Xia,Xia Zhang,Huan Jiang,Baihui Wang,Peilei Zhang,Yujin Zhang,Yanxue Tang,Yi Zhou
A novel Au NPs/GeO 2 nanozymes are developed as Surface-Enhanced Raman Scattering (SERS) substrates with the promising prospect for detection ChI. Herein, it is discovered that both Au NPs and GeO 2 nanozymes have peroxidase-like activity, catalyzing colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue TMB ox . Interestingly, compared with single Au NPs or GeO 2 nanozymes, the Au NPs/GeO 2 nanozymes show stronger peroxidase-like activity, and significantly ameliorated SERS signal of TMB ox . The mentioned two enhancements are ascribed to a positive synergistic function of Au NPs/GeO 2 nanozymes. Surprisingly, choline iodide (ChI) can inhibit the positive synergy in Au NPs/GeO 2 nanozymes, and slow down the reaction of TMB-H 2 O 2 -Au NPs/GeO 2 system. On this foundation, a new Au NPs/GeO 2 SERS technique with high sensitivity, label-free detection method of choline iodide (ChI) is established, suggesting that Au NPs/GeO 2 nanozymes have the potential application of water environment.
研究领域:
生物化学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Shao-Bin He,Hui-Jing Yang,Liu Yang,Hamada A. A. Noreldeen,Hua-Ping Peng,Hao-Hua Deng,Wei Chen,Guo-Lin Hong
Coenzyme can work with a natural enzyme to achieve the enzymatic catalytic process. Inspired by nature, herein rutin was found to regulate the peroxidase-like activity of Fe-doped silicon nanodots (Fe-OSiNDs) acting as a coenzyme. To be specific, the peroxidase-like activity of Fe-OSiNDs can be activated by rutin at a low concentration (<200 µM), which accelerated the oxidation process between 3, 3′, 5, 5′-tetramethylbenzidine (TMB) and H 2 O 2 . Possibly as a reason, rutin at a low concentration could be easily attracted and oxidized by Fe 3+ in Fe-OSiNDs and then the produced Fe 2+ reacted with H 2 O 2 to form the hydroxyl radicals around the surface of Fe-OSiNDs thereby contributing to an enhancement. More interestingly, the addition of β-glucuronidase can hydrolysis rutin into quercetin and suppress the peroxidase-like activity of rutin-treated Fe-OSiNDs because quercetin is a sugar-free flavonoid with higher anti-oxidant activity than rutin. Thus, a colorimetric sensor for β-glucuronidase could be established. This sensor realizes a linear ranging from 2 to 40 nM (0.58 to 11.6 μg/mL) towards β-glucuronidase with a detection limit of 0.6 nM (0.174 μg/mL). The feasibility of this sensor for β-GUS detection in Escherichia coli has been realized. This work not only provides an interesting concept for coenzyme-dependent nanozyme and expands the promising use of silicon nanomaterials.
研究领域:
生物化学
·
纳米生物学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Donglian Deng,Jingyuan Xu,Tingting Li,Dongdong Tan,Yibing Ji,Ruijun Li
This work designed a fluorometric/colorimetric dual-mode sensor for detecting 2,6-dipicolinic acid (DPA) based on the blue emission property and peroxidase-like activity of Fe-MIL-88NH 2 . The fluorescence of Fe-MIL-88NH 2 was obviously turned off by Cu 2+ , but DPA was able to bring it back because it has a strong chelate bond with Cu 2+ . Fe-MIL-88NH 2 also displayed high peroxidase-like activity, which accelerated the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue oxidation product (oxTMB) when H 2 O 2 was present. When DPA was added, it efficiently inhibited the peroxidase-like activity of Fe-MIL-88NH 2 , causing less oxTMB and less absorbance at 652 nm. The fluorescence recovery of Fe-MIL-88NH 2 and the change in absorbance at 652 nm were used as analytical signals for dual-mode detection of DPA. The linear responses in the range of 10–60 μM and 60–160 μM were achieved for the fluorometric mode, and the limit of detection (LOD) was 1.46 μM. The respective values of linear range and LOD for the colorimetric mode were 5–25 μM and 3.00 μM, respectively. In summary, the dual-mode testing strategy successfully detected DPA in aqueous environmental samples, suggesting great potential in disease prevention and environmental analysis.
研究领域:
分析化学
·
环境科学
·
生物传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）