CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Zheng Chen,Jiulong Zhao,Hang Wu,Haibin Wang,Xuhua Lu,Mohammad-Ali Shahbazi,Shige Wang
Hydrogel is a kind of hemostatic agent with good application prospect. However, the water molecules on the wound made the hydrogel less adhesive to wet wound tissue. Herein, the carboxymethyl chitosan (CMCS)/oxidized dextran (OD)/γ-polyglutamic acid (γ-PGA) hydrogel was prepared using a double-barreled syringe for hemostasis of diffuse and incompressible wound bleeding. The hydrogel formation was based on the intramolecular lactam bonds, intermolecular amide bonds, and Schiff base bonds. In the hydrogel, the super hydrophilic γ-PGA could drain the surface moisture of the wound and create a local dry environment for enhanced surface adhesion. In vivo study showed that the CMCS/ODex/γ-PGA hydrogel possesses a good biosafety and biodegradability . Interestingly, the CMCS/ODex/γ-PGA hydrogel exhibited excellent hemostatic abilities in dynamic humid environment and resisted a high blood pressure of 238 mmHg, which exceeds the threshold systolic blood pressure of healthy adults ( i.e. , 120 mmHg). Together with the antibacterial and reactive nitrogen species scavenging activities, this study is expected to provide a new method to design the wet-surface adhesives for the efficient hemostatic application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Lumin Yang,Xiaoduo Zhao,Yunsong Kong,Renjie Li,Tao Li,Rui Wang,Zhengfeng Ma,Yong-min Liang,Shuanhong Ma,Feng Zhou
The typical symptoms of arthritis are inflammation and lubrication deficiency in joints, which increase wear of articular cartilage along with pain of patients. In the present study, one kind of novel macromolecular/microsphere-based injectable hydrogels (CMC-ODex NPs) with dual functionalities of drug release and lubrication, was fabricated via dynamic Schiff base crosslinking network between carboxymethyl chitosan (CMC) and oxidation dextran nanoparticles (ODex NPs). The CMC-ODex NPs hydrogels exhibited typical viscosity-thinning phenomenon at wide range of shear rates and obvious gel-sol transition feature at specific strain. As a result, CMC-ODex NPs hydrogels presented low friction coefficient at the sliding interface of bovine articular cartilages, resulting from the boundary lubrication of hydrogel and the rolling friction effect of ODex NPs. Furthermore, the anti-inflammatory drug (dexamethasone, DXM) encapsulated in ODex NPs exhibited sustainable drug release behavior during the dynamic shearing process, which making CMC-ODex NPs hydrogels possessed good and stable anti-inflammatory effect. CMC-ODex NPs hydrogels was prepared without utilizing any toxic agents, thus demonstrated excellent cytocompatibility. Our experimental results reveal the CMC-ODex NPs hydrogels is promising to be used as functional lubricant for inhibiting the development of arthritis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 18
·3404-3411
·
IF 3.614
Yudan Wang,Cheng Zhang,Lei Zhang,Zhen OuYang,Ming Zhao,Jiaoyang Luo,Meihua Yang
In this study, a sensitive and accurate immunoaffinity columns coupled with high-performance liquid chromatography method was established to monitor the presence of aflatoxins—aflatoxin B 1 , aflatoxin B 2 , aflatoxin G 1 , and aflatoxin G 2 —in different medicinal herbs. The proposed method was found to be suitable for the detection of aflatoxins in eight kinds of herbs and their corresponding granules. Two batches of Arecae semen were positive for aflatoxins, with high residue levels of different aflatoxins. To better understand the presence and transfer of aflatoxins during the formulation of dispensing granules from the herbs, the aflatoxins-free herbs were artificially inoculated with Aspergillus flavus to explore aflatoxins production. Both aflatoxin B 1 and aflatoxin B 2 were detected in all inoculated samples, while aflatoxin G 2 was only detected in Astragali radix samples. Additionally, the presence of aflatoxin B 1 was extremely high compared to other aflatoxins. More specifically, the transfer rate of the aflatoxin B 1 and the total aflatoxins from original herbs to granules were both approximately 40%. These findings indicated that the preparation of herbs into dispensing granules reduced the content of aflatoxins. The high-level presence of aflatoxins in inoculated herbs indicated that attention is needed to the safety of A. flavus -contaminated herbs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 13
·6928
·
IF 6.208
Meixing Wang,Huizhen Hu,Buyu Zhang,Yang Zheng,Pan Wu,Zhenghui Lu,Guimin Zhang
Panose is a type of functional sugar with diverse bioactivities. The enzymatic conversion bioprocess to produce high purity panose with high efficiency has become increasingly important. Here, a new neopullulanase (NPase), Amy117 fromB. pseudofirmus703, was identified and characterized. Amy117 presented the optimal activity at pH 7.0 and 30 °C, its activity is over 40% at 10 °C and over 80% at 20 °C, which is cold-active. The enzyme cleaved α-1, 4-glycosidic linkages of pullulan to generate panose as the only hydrolysis product, and degraded cyclodextrins (CDs) and starch to glucose and maltose, with an apparent preference for CDs. Furthermore, Amy117 can produce 72.7 mg/mL panose with a conversion yield of 91% (w/w) based on 80 mg/mL pullulan. The sequence and structure analysis showed that the low proportion of Arg, high proportion of Asn and Gln, and high α-helix levels in Amy117 may contribute to its cold-active properties. Root mean square deviation (RMSD) analysis also showed that Amy117 is more flexible than two mesophilic homologues. Hence, we discovered a new high-efficiency panose-producing NPase, which so far achieves the highest panose production and would be an ideal candidate in the food industry.Keywords:neopullulanase (NPase);pullulan;panose production;high-efficiency
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jiaqi Xing,Yuan Ding,Xiaoran Zheng,Peng Yu,Meng Qin,Rongmin Qiu,Yanyan Li,Siyuan Shang,Jing Xie,Jianshu Li
Oral ulcers (OUs) are the most common lesions of the oral mucosa and severely reduce patients' quality of life. Several types of patches for the self-treatment of OU have been developed, which have partially alleviated the problems caused by OUs. However, their wide application is limited by a few factors, including the suboptimal adhesion to the wound in the complex oral environment and the discoloration of the degraded products. Herein, an ideal Janus patch for the accelerated treatment and cure of OUs, comprising bifunctional sides, was designed. Inspired by the composition and hybrid adhesive mechanism of barnacles, glycine was modified and combined with hydroxyapatite nanoparticles (HAp) to create an adhesive layer that would form abundant covalent bonds with tissues; the HAp act as a bridge to bond polymer chains mimicking the organic–inorganic hybrid mechanism of barnacles, achieving analogous instant wet adhesion. Additionally, double bond-modified gellan gum served as a slippery layer to avoid undifferentiated adhesion and secondary damage. When applied to rat OUs, the Janus patch exhibited instant wet adhesion, with the longest residue time, overcoming the limitations of commercial products, and withstood chewing and biting and other mouth movements, thus achieving an excellent therapeutic efficacy.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 3
·1258-1264
·
IF 4.036
Zengchen Liu,Like Wang,Baodui Wang,Yahong Chen,Fengshou Tian,Yingying Xue,Yanxia Li,Wenping Zhu,Weijie Yang
As a class of important carbon nanomaterial, carbonized polymer dots (CPDs), also called carbon dots (CDs), have aroused wide interest owing to their unique water solubility, fluorescence properties, and rich surface functional groups. However, the directional tuning of the fluorescence properties of CPDs remains incomplete because of the influence of many factors like diameter, solvent and surface groups. Particularly, most carbonized polymer dots are synthesized in a neutral pH environment. Herein, by modulating the pH (strongly acidic or alkaline) of dextrin water solution, bicolor fluorescence emission (blue and yellow) CPDs were prepared by a hydrothermal reaction. Through systematic characterization, it was found that the different fluorescence properties are regulated by the diameters and surface groups of the carbon cores. Simultaneously, the pH value affected the nucleation process. Based on the excellent fluorescence properties, cell fluorescence imaging and cytotoxicity were tested. The bicolor fluorescence CPDs obtained by tuning the pH provide an important theoretical basis for the design of broadband CPDs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Weizhe Tang,Tong Zhao,Rui Dou,Li Wang
We prepare bimodal particle size photo-curable ceramic pastes with high solid loadings (up to 65 vol %) and fabricate porous alumina ceramic cores with complex shapes via ceramic stereolithography (Cer-SLA) 3D printing technique. The sintering temperature is carefully selected, ranging from 1500 °C to 1650 °C, and a high holding time (>4 h) is applied to guarantee that the materials can withstand the subsequent high temperature (>1500 °C) casting process for single-crystal nickel-based superalloy hollow turbine blades. Herein, the originally spherical fine particles are found to become platelet-like after sintering, and the forming mechanism is discussed in detail. In addition, we explore the influence of platelet-like particles, coarse particles and sintering process on the microstructural evolution of alumina particles, and reveal the relationship between microstructure and properties of ceramic cores. These results illustrate that the proposed materials for SLA 3D printing exhibit a great potential in the fabrication of complex-shaped alumina ceramic cores for high-precision investment casting, e.g., manufacturing single-crystal nickel-based superalloy hollow turbine blades for an advanced aircraft engine.
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Yuangan Chen,Bo Feng,Huashan Yan,Liangzhu Zhang,Chunhui Zhong,Tao Wang,Huihui Wang,Longhua Xu
In this work, dextrin , an effective and eco-friendly organic compound, was used to separate fluorite from calcite . The depression performance and adsorption mechanism of it on the calcite surface were investigated by flotation experiments, contact angle measurements, Fourier transform infrared spectroscopy (FTIR) measurements, and X-ray photoelectron spectroscopy (XPS) analyses. The results showed that fluorite can be selectively separated from calcite with 10 mg/L dextrin and 2 × 10 −5 mol/L sodium laurylsulfonate (SLS). Additionally, the contact angle, FTIR and adsorption measurements indicated that the –OH group of dextrin might be adsorbed on Ca sites on the surface of calcite through chemical bonds. The inner electron binding energy of Ca 2+ in fluorite is larger than the inner electron binding energy of Ca 2+ in calcite, which makes it more difficult to form chemical bonds. Therefore, the chemical adsorption of dextrin on the surface of calcite is stronger than that on the surface of fluorite. Both SLS and dextrin can be adsorbed on the surface of calcite. However, dextrin is a strong hydrophilic compound, the hydrophilicity caused by dextrin is stronger than the hydrophobicity caused by SLS, so dextrin can selectively depress calcite.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Simin Liu,Jiamei Liu,Linguo Zhao,Jianjun Pei
Glycosylation, one of the most common and significant modifications in nature, has prompted the development of a cellobiose phosphorolysis route for glycosylation in vivo. However, the process of glycosylation is hampered by the notably low conversion rate of cellobiose. In this work, regulation of the carbon source supply by changing the ratio of glucose to cellobiose improved the conversion rate of cellobiose, resulting in enhancing the efficiency of glycosylation and the production of vitexin. Moreover, three genes (pgm, agp, and ushA) involved in the degradation of UDP-glucose were knocked out to relieve the degradation and diversion of the cellobiose phosphorolysis route. Finally, through the optimization of conversion conditions, we observed a continuous enhancement in cellobiose conversion rate and vitexin production in BL21ΔushAΔagp-TcCGT-CepA, corresponding to an increased concentration of added glucose. The maximum production of vitexin reached 2228 mg/L with the addition of 2 g/L cellobiose and 6 g/L glucose, which was 312% of that in BL21-TcCGT-CepA with the addition of 2 g/L cellobiose. The conversion rate of cellobiose in BL21ΔushAΔagp-TcCGT-CepA reached 88%, which was the highest conversion rate of cellobiose to date. Therefore, this study presents a cost-effective and efficient method to enhance the conversion rate of cellobiose during the glycosylation process.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2023
·
IF 9.7
Lin Liu,Min Liu,Jingya Xiu,Bowen Zhang,Haiyang Hu,Mingxi Qiao,Dawei Chen,Jiulong Zhang,Xiuli Zhao
Depression is one of the most common mental diseases, which seriously affects patients’ physical and mental health. Emerging evidence has indicated that oxidative stress (OS) is a major cause of neurodegeneration involved in the pathogenesis of depression. Consequently, targeted reactive oxygen species (ROS) elimination is regarded as a promising strategy for efficient depression therapy. In addition, insufficient brain drug delivery is the main obstacle to depression therapy owing to the presence of the blood-brain barrier (BBB). To achieve the goals of bypassing the BBB and promoting antioxidant therapy for depression, a broad-spectrum ROS scavenging NPs was rationally designed through a nasal-brain pathway developed for combined ROS scavenging and brain drug delivery. A hexa-arginine (R6) modified ROS-responsive dextran (DEX) derivate was synthesized for antidepressant olanzapine (Olz) and H 2 donor amino borane (AB) loading to prepare Olz/RDPA nanoparticles (NPs). Subsequently, the NPs were dispersed into a thermoresponsive hydrogel system based on poloxamer. In vitro and in vivo results demonstrated that Olz/RDPA in situ thermoresponsive hydrogel system could effectively deliver NPs to the brain via the nasal-brain pathway and alleviate depression-like behaviors through combined ROS depletion and inhibition of 5-HT dysfunction of the oxidative stress-induced. The proposed ROS-scavenging nanotherapeutic would open a new window for depression treatment. Statement of Significance ROS is an innovative therapeutic target involving the pathology of depression whereas targeted delivery of ROS scavenging has not been achieved yet. In the current study, ROS-responsive nanoparticles (Olz/RDPA NPs) were prepared and dispersed in a thermosensitive hydrogel for delivery through the nasal-brain pathway for the treatment of depression. Sufficient ROS depletion and improvement of delivery capacity by the nasal-brain pathway effectively could reverse oxidative stress and alleviate depressive-like behavior. Collectively, these nanoparticles may represent a promising strategy for the treatment of depression.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
·
2023
·
IF 9
Run Qi,Zhaohao Li,Hongyuan Zhang,Hongming Fu,Heng Zhang,Dan Gao,Haiping Chen
Membrane absorption is a promising CO 2 capture technology, which is limited by the high cost of membrane materials. Coal fly ash (CFA)-based ceramic membrane as an alternative material for membrane modules can effectively reduce the preparation cost. In this study, CFA-based ceramic membrane with superhydrophobic properties is prepared by depositing SiO 2 nanoparticles and grafting 1H, 1H, 2H, 2H-perfluorooctyltriethoxysilane (POTS). Characters including pore size distribution, porosity and N 2 flux of superhydrophobic membrane are performed. The superhydrophobic modified ceramic membrane is applied to CO 2 capture experiments using ethanolamine (MEA) solution as the absorbent. The result shows that the contact angle of the modified ceramic membrane reaches 155.0°, which significantly improves the anti-wetting properties of the membrane surface . The CO 2 capture experiment demonstrates a maximum capture efficiency of 97.45% and a mass transfer rate of 21.17 mol/(m 2 ·h). In addition, the superhydrophobic modified ceramic membrane exhibits excellent thermal and chemical stability, sustaining high performance even after 8 h of continuous operation. This study provides the theoretical and empirical foundation for the application of CFA-based superhydrophobic ceramic membranes in CO 2 capture.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 20
·3814
·
IF 5.2
Jiahang Yu,Mingyuan Huang,Huixin Tian,Xinglian Xu
The series of biochemical reactions, metabolic pathways, and regulatory interactions that occur during the storage of meat are the main causes of meat loss and waste. The volatile compounds produced by these reactions, such as hydrogen sulfide, acids, and amines, can directly indicate changes in the freshness of meat during storage and sales. In this study, a one-pot hydrothermal method based on a surface control strategy was used to develop nanoparticles of silver with different reactivities, which were further immobilized in agar powder to develop a colorimetric sensor array. Due to the different chemical interactions with various volatile compounds, the colorimetric sensor array exhibited distinct color changes. The study demonstrates significant differences between 12 different volatile compounds and provides a quantitative and visual method to reveal rich detection indicators. The colorimetric sensor array is an economical and practical multi-analyte identification method. It has many potential applications such as food packaging, anti-counterfeiting, health monitoring, environmental monitoring, and optical filters.Keywords:colorimetric sensor array;Ag NPs;VOCs;nanosensors;chilled broiler meat
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）