COMBUSTION AND FLAME
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2019
·
IF 4.12
Bin Hu,Qiang Lu,Zhen-Xi Zhang,Yu-Ting Wu,Kai Li,Chang-Qing Dong,Yong-Ping Yang
The fast pyrolysis mechanism of xylose , xylobiose and xylan was investigated with combined fast pyrolysis experiments and quantum chemistry calculations. The results indicate that acyclic d -xylose is an important intermediate for the formation of the major pyrolytic products in xylose pyrolysis, i.e., 1,4-anhydro- d -xylopyranose (ADX), furfural (FF) and hydroxyacetaldehyde (HAA). ADX derives from the successive cyclization of d -xylose which is the only source for ADX formation. 3-Deoxy-xylose is the most important intermediate for FF formation. HAA results from the C C bond scission by retro-aldol and cyclic Grob fragment. In addition, the five-membered intermediate derived from the ring-contraction reaction can transform into FF with high selectivity. The dehydrated xylose is also another source for HAA formation. The fast pyrolysis mechanism of xylobiose is similar to that of xylose, and thus similar pyrolytic product distribution can be obtained from them. However, the pyrolytic products of xylan differ significantly from those of xylose and xylobiose, which is ascribed to its high degree of polymerization and the branches in it.
研究领域:
化学工程
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生物质能
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DOI
高影响因子
JOURNAL OF CHEMICAL SCIENCES
·
2020
·Vol. 132
·期 1
·1-9
·
IF 1.406
Ning Wensheng,Li Bei,Dai Hui,Hu Shiye,Yang Xiazhen,Wang Biao,Zhang Bo
Abstract The influences of sugars (sucrose, fructose and glucose) on the performance of FeAl catalysts were investigated for CO 2 hydrogenation. FeAl catalysts were prepared with two steps. At first, the catalyst precursors were obtained by co-precipitation.
During this step, three methods were used to add sucrose into the precursors. Then, promoter K and Cu were impregnated into the precursors. The improved CO 2 conversion and C 5+ hydrocarbon selectivity by sucrose addition were attributed to the formation of γ-Fe 2 O 3 phase in the catalyst precursor, which was different from the popular opinion that sucrose acted as a chelating agent. With the inspiration from sucrose hydrolysis effect, FeAl oxide, mainly in γ-Fe 2 O 3 phase was prepared by adding fructose and glucose (the products of sucrose hydrolysis) into the newly centrifuged precipitate. The formation of γ-Fe 2 O 3 phase was explained based on the results of XRD and XPS. The best catalyst possessed CO 2 conversion of 30.3% and C 5 + selectivity of 52.2% under the reaction conditions of H 2 :CO 2 = 3:1, 6.0 L/(h·g-cat), 1.6 MPa and 235 °C.
Graphic abstract
Effects of fructose and glucose on FeAl catalyst
研究领域:
化学工程
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催化科学
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DOI
高影响因子
Marine Drugs
·
2019
·Vol. 17
·期 4
·195
·
IF 3.772
Ruowei Guan,Yuan Peng,Lutan Zhou,Wenqi Zheng,Xixi Liu,Pin Wang,Qingxia Yuan,Na Gao,Longyan Zhao,Jinhua Zhao
Apostichopus japonicusis one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan fromA. japonicus(AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula ofl-FucS-α1,3-d-GlcA-β1,3-{d-GalNAc4S6S-β1,4-[l-FucS-α1,3-]d-GlcA-β1,3-}n-d-anTal-diol4S6S(n= 0, 1, 2, 3; FucS represents Fuc2S4S, Fuc3S4S, or Fuc4S). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-β1,3-d-GalNAc4S6S-β1-}n, which is similar to chondroitin sulfate E, and eachd-GlcA residue in the backbone was branched with al-FucS monosaccharide atO-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.Keywords:Apostichopus japonicus;fucosylated glycosaminoglycan;oligosaccharide;unambiguous structure;anti-Fxase
研究领域:
海洋生物学
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药理学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Zhen Li,Qiang Huang,Yu Zheng,Yong Zhang,Xin Li,Shiqing Zhong,Zhijiang Zeng
Identifying the components of Camellia oleifera honey and pollen and conducting corresponding toxicological tests are essential to revealing the mechanism of Camellia oleifera toxicity to honey bees. In this research, we investigated the saccharides and alkaloids in honey, nectar, and pollen from Camellia oleifera, which were compared with honey, nectar, and pollen from Brassica napus, a widely planted flowering plant. The result showed that melibiose, manninotriose, raffinose, stachyose, and lower amounts of santonin and caffeine were found in Camellia oleifera nectar, pollen, and honey but not in B. napus nectar, pollen, and honey. Toxicological experiments indicated that manninotriose, raffinose, and stachyose in Camellia oleifera honey are toxic to bees, while alkaloids in Camellia oleifera pollen are not toxic to honey bees. The toxicity mechanism of oligosaccharides revealed by temporal metabolic profiling is that oligosaccharides cannot be further digested by honey bees and thus get accumulated in honey bees, disturbing the synthesis and metabolism of trehalose, ultimately causing honey bee mortality.
研究领域:
昆虫学
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毒理学
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植物科学
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农业科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·67-77
·
IF 3.713
Mengqing Zhang,Ruijin Yang,Shuhuai Yu,Wei Zhao
Summary α-Glucosidase inhibitors, which can inhibit α-glucosidase to reduce the release of glucose after a mixed carbohydrate diet, has become a key tool in the management of type 2 diabetes mellitus (T2DM). SFP-1, an α-glucosidase inhibitor derived from Sargassum fusiforme ( S . fusiforme ), was studied as a food-derived hypoglycaemic agent. By binding α-glucosidase and then changing the conformation of the enzyme, SFP-1 with a mixed inhibition type exerted an excellent inhibitory effect (IC 50 value: 0.681 mg mL −1 ). Structural analyses suggested that the average molecular weight of SFP-1 was 1.61 × 10 5 Da, mainly composed of glucuronic acid, fucose, galactose, glucose, mannose and xylose, and the relative molar ratio was 23.07: 15.17: 7.72: 4.92: 4.04: 1.99. FTIR and NMR analysis suggested that SFP-1 was an acidic polysaccharide with both α-type and β-type glycosidic bonds. S . fusiforme polysaccharide (SFP-1) has the potential to develop as functional foods and nutraceuticals in T2DM management.
研究领域:
食品科学
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药理学
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海洋生物学
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DOI
高影响因子
BIOMASS & BIOENERGY
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2025
·
IF 5.8
Jingliang Xu,Shiwei Luo,Yaxian Qiao,Chunyu Li,Yuduo Zheng,Mohammad Asraful Alam,Shen Zhang
Mannose exhibits specific biological functions and can be acted as a precursor for many drugs. In this study, we used glucose as substrate for preparing mannose by thermos-chemical synthesis. Carbon nitride (C3N4) supported molybdenum and aluminum was prepared to selectively convert glucose to mannose and fructose in water. Results show that the yield and selectivity of mannose from glucose are 31.47 % and 85.00 %, respectively, at 130 °C for 5 min by C3N4 supported molybdenum (Mo-C3N4). The catalytic efficiency of Mo-C3N4 shown to be high. The yields of mannose and fructose are 18.72 % and 41.34 %, correspondingly and the Al-Mo-C3N4 exhibits a total selectivity of 91.91 % for mannose and fructose. C3N4 provided Brønsted bases (N-containing groups) facilitate isomerization of glucose to fructose. The Mo-C3N4 characterizations reveal that the 2D nanosheet structure embeds Mo species (MoO2, MoO3 and MoC2) at a size of 5–10 μm. The characterizations of Al-Mo-C3N4 proved the hierarchically porous structure and the homogeneous distributions of Al species (Al 2 O 3 ) and Mo species (Mo 4+ and Mo 6+ ) throughout the C3N4 skeletons. The work reveals a green and low-cost catalyst based on C3N4 support for highly selectivity catalyzing the conversion of glucose to mannose and fructose in aqueous.
研究领域:
化学
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生物学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yuanyuan Zhao,Yubin Wang,Qiancheng Ma,Dan Wang,Qianqian Jiang,Pan Wang,Zhiwen Ge,Junjuan Wang,Peiyou Qin,Xiaoyan Zhao
Dietary oligo- and polysaccharides modulate gut microbiota and thus exert prebiotic activity, which is determined by their heterogeneous structure. To explore the correlations between monosaccharide profile and microbial community, simulated gut fermentation of different glycans, including arabinan (ArB), galactooligosaccharide (GOS), arabinogalactan (ArG), rhamnogalacturonan (RhG), and xyloglucan (XyG) that are characterized by typical sugar residues were performed. Results showed that RhG displayed high contents of galacturonic acid (344.79 mg/g), rhamnose (171.70 mg/g), and galactose (151.77 mg/g), and the degradation ratio of them after fermentation was 73.87 %, 84.96 %, and 87.11 %, respectively. Meanwhile, the relative abundance of glycan-degrading bacteria Bacteroides in the RhG was boosted from 4 h (4.97 %) to 48 h (36.45 %). Butyrate-generating bacteria Megasphaera (56.69 %) and Bifidobacterium (28.02 %) are dominant genera in the ArB, which generated the highest concentration of carbohydrate-metabolite (94.58 mmol/L) in terms of acetate, propionate, butyrate and valerate, followed by the ArG (87.36 mmol/L). However, ammonia generation of the ArG increased rapidly, representing the highest content of protein-metabolite (66.36 mmol/L) including ammonia, isobutyrate, and isovalerate. As compared, metabolites generated from protein and carbohydrates grow steadily at a low level during the XyG fermentation. Correlation analysis further indicated that Bacteroide s was positively correlated with propionate ( p < 0.001), galacturonic acid ( p < 0.001), and rhamnose ( p < 0.05), while Bifidobacterium has positive correlation with butyrate and arabinose ( p < 0.01). Overall, monosaccharides composition in the different oligo- and polysaccharides induces distinct responses of the dominant microbiota and thus modulates the subsequent fermentation metabolites of carbohydrate and protein, promoting a deep understanding of the structure-fermentation relationship of dietary glycans.
研究领域:
微生物学
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营养学
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食品科学
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肠道微生物
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yongchun Huang,Jie Bai,Jinman Shui,Zhanhai Su,Chengzhu Cao,Haiyan Wang,Qiong Wu,Shoude Zhang
Analysing monosaccharide composition is an essential step in deciphering polysaccharide structures. However, it presents significant challenges due to the pronounced hydrophilicity and the vast array of monosaccharide isomers. Gas/liquid chromatography, coupled with an aldononitrile acetate derivatisation, has been widely used for monosaccharide composition analysis. However, it poses considerable difficulties in simultaneously determining the D/L configurations of these monosaccharides. In this study, a novel method that can simultaneously determine the composition of polysaccharides and their D/L configuration based on L-cysteine methyl ester derivatisation (CMED) and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the comprehensive analysis of monosaccharide types. This method enables the simultaneous characterization of aldoses, ketoses, alditols, and uronic acids. Furthermore, the reaction mechanisms and by-products were meticulously analysed, offering a robust theoretical basis for this novel methodology. Additionally, the developed method was employed to characterize the monosaccharide composition for six plant polysaccharides. Ultimately, the findings demonstrated that the proposed method is effective for confirming monosaccharide composition, encompassing both structural types and absolute configurations.
研究领域:
分析化学
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碳水化合物化学
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生物化学
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植物科学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 5
·1457
·
IF 4.927
Ying Zhang,Yanna Pan,Jiaqi Li,Zeng Zhang,Yanming He,Hongjie Yang,Ping Zhou
The prevention of postprandial hyperglycemia and diabetic complications is crucial for diabetes management. Inhibition of α-glucosidase to slow carbohydrate metabolism is a strategy to alleviate postprandial hyperglycemia. In addition, suppression of non-enzymatic glycation can diminish the advanced glycation end products and reduce the oxidative stress and inflammation, thereby preventing the diabetic complications. In this study, an anti-oxidative proteoglycan (namedFYGL) extracted fromGanoderma lucidumwas investigated in vitro for its inhibitory effect on α-glucosidase and non-enzymatic glycation using molecular kinetics, intrinsic fluorescence assay, and bovine serum albumin glycation models. The molecular kinetics and fluorescence assay revealed thatFYGLdecreases α-glucosidase activity by forming aFYGL–α-glucosidase complex. To evaluate the anti-glycation effect, fructose-glycated and methylglyoxal-glycated BSA models were analyzed by spectroscopic and SDS-PAGE methods. Results showed thatFYGLinhibited the glycation at every stage and suppressed glycoxidation, possibly due to its anti-oxidative capacity andFYGL–BSA complex formation. Furthermore, we demonstrated in vivo thatFYGLcould alleviate postprandial hyperglycemia indb/dbmice as well as AGE accumulation and vascular injury in diabetic rats. Overall,FYGLpossesses anti-postprandial hyperglycemia and anti-glycation functions and would be potentially used in clinic for diabetes and related complication management.Keywords:Ganoderma lucidum;postprandial hyperglycemia;α-glucosidase;non-enzymatic glycation;advanced glycation end products (AGEs)
研究领域:
药理学
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糖尿病研究
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ping Yang,Xumei Wang,Hui Wang,Yueming Hu,Pingwei Wen,Zongcai Tu
The mechanism of the effect of reducing sugar chain length and isomers on the allergenicity of Ara h 2 after glycation was investigated. Ara h 2 was more prone to glycation with ribose which had a short chain length. The glycation sites of Ara h 2 after glycation with galactose were higher than the glycation sites in galactose’s isomers-Ara h 2 conjugates, which might be affected by the configuration differences at position C-4 and the small steric effects in terminal groups –CHO of galactose. Ara h 2-ribose conjugate had the lowest allergenicity, and glycation with galactose was more capable of reducing Ara h 2 allergenicity than its isomers. The results indicated that glycation with ribose caused conformational epitope destruction and linear epitope masking of Ara h 2 greatly. Furthermore, since the small steric effects of –CHO, galactose was more capable of reducing Ara h 2 allergenicity than fructose. This study will provide a theoretical basis for selecting appropriate reducing sugars and preparing hypoallergenic products containing peanuts.
研究领域:
食品科学
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过敏学
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生物化学
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DOI
PubMed
高影响因子
ACS Chemical Neuroscience
·
2022
·
IF 5.78
Mingming Xu,Hong Jin,Zhen Wu,Ying Han,Jing Chen,Chengjie Mao,Piliang Hao,Xumin Zhang,Chun-Feng Liu,Shuang Yang
It is urgently needed to find reliable biofluid biomarkers for early diagnosis of Parkinson’s disease in order to achieve better treatment. Promising biomarkers can be found in Parkinson’s disease-related glycoproteins as aberrant protein glycosylation plays an important role in disease progression. However, current information on serum N-glycoproteomic changes in Parkinson’s disease is still limited. Here, we used glycoproteomics methods, which combine the solid-phase chemoenzymatic method, lectin affinity chromatography, and hydrophilic interaction chromatography with high-resolution mass spectrometry, to analyze the glycans, glycosites, and intact glycopeptides of serum. Increased abundance of glycans containing core fucose, sialic acid, and bisecting N-acetyl glucosamine was detected at the overall glycan level and also at specific glycosites of glycopeptides. Five Parkinson’s disease-associated proteins with this type of N-glycosylation changes were also identified. We propose that the revealed site-specific N-glycosylation changes in serum can be potential biomarkers for Parkinson’s disease.
研究领域:
神经学
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生物化学
·
蛋白质组学
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疾病生物标志物
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yu Lu,Wanjun Jin,Yuerong Yang,Yue Jia,Lujia Sun,Jie Liu,Langhong Wang,Fuxing Zhang,Wupeng Ge,Jiansheng Wang,Linjuan Huang,Zhongfu Wang
Goat milk oligosaccharides are complex carbohydrates with a variety of biological functions. Free oligosaccharides from goat milk show more similarity to human milk than cow milk. At present, changes in goat milk glycoconjugates at different parities remain poorly studied. Herein, we qualitatively and quantitatively compared the goat milk glycoprotein N/O-glycome at different parities using a stable isotope labeling followed by electrospray ionization mass spectrometry and online hydrophilic interaction chromatography. N-Glycans were mainly fucosylated and nonfucosylated nonsialylated, and both fucosylation and sialylation gradually increased with parity, amounting (at the third parity) to 1.25 times and 3.3 times those of the first parity, respectively. O-Glycans were mostly nonfucosylated and nonsialylated, and sialylation increased with increasing parity, and Neu5Ac-sialylated was up to 9 times higher in the third parity than in the first parity, whereas Neu5Gc-sialylated was 5.5 times higher. This study provides a reference for exploring an alternative milk source closest to human milk and for the development of humanized formula milk.
研究领域:
食品科学
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生物化学
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营养学
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DOI
高影响因子
危险品化学品经营许可证（带存储）