SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Qian Kuang,Chunli Li,Zhiwei Qiu,Guifen Jie,Shuyan Niu,Tingyu Huang
In this work, a target-triggered three-way junction (3-WJ) structure was combined with enzyme-powered cascade amplification strategy for ultrasensitive electrochemiluminescence (ECL) detection of microRNA via free-running DNA walker. In the presence of target microRNA-21 (miRNA-21), a three-way junction structure was formed to trigger enzyme-aided multiple DNA amplification, and thus exponentially amplified triggers were generated. Then, the trigger DNA could hybridize with the blocking DNA on the electrode to release walker DNA, which specifically combined with the quantum dots (QDs)-DNA probe, and formed a recognition site for Nt.BbvCI. The movement of DNA walker is powered by the nicking endonuclease that cleaves specific QDs-DNA probes on the track. Thus one walker DNA can automatically cleave multiple QDs probes on the electrode during the movement, resulting in significantly amplified changes of ECL signal, which could be used for the highly sensitive detection of target miRNA-21. Under the optimal conditions, the ECL signal change is linear with the concentration of miRNA-21 in the range from 10 −14 M to 10 -7 M with a detection limit of 1.5 fM. This newly established strategy could achieve rapid, isothermal, and homogeneous signal amplification for specific nucleic acids in complicated biomatrix, which hold great potential for application in early clinical diagnosis.
研究领域:
生物化学
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分子生物学
·
分析化学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Youxiong Zhang,Xianhu Wei,Qihui Gu,Jumei Zhang,Yu Ding,Liang Xue,Moutong Chen,Juan Wang,Shi Wu,Xiaojuan Yang,Shuhong Zhang,Tao Lei,Qingping Wu
Robust and efficient multimodal catalysis platforms that possess high catalytic activity and enhanced electrical conductivity are preferred for enhancing the electrical analysis performance under a low overpotential. This report describes a function-switchable amperometric sensor for electrochemical monitoring hydrogen peroxide and glucose under relatively low overpotential based on PEI-functionalized metal–organic framework (P-MOF) supported gold nanoparticles/nitrogen-doped graphene quantum dots (AuNPs/N-GQDs) and glucose oxidase. AuNPs/N-GQDs with high peroxidase mimicking activity was anchored on the P-MOF modified electrode surface as H 2 O 2 sensors, exhibiting a high sensitivity of 134.26 μA mM −1 cm −2 and a detection limit of 3.38 μM. Subsequently, AuNPs/N-GQDs-P-MOF used as a nanocarrier for glucose oxidase (GOx) to achieve “cascade nanoreactor” amplification for glucose detection. The amplified amperometric glucose biosensor showed excellent anti-interference ability, reproducibility, and a limit of detection as low as 0.7 μM (S/N = 3) with high sensitivity of 1512.4 μA mM −1 cm −2 . In addition, the feasibility of the electrochemical biosensor for accurate and quantitative detection of glucose in human serum samples was confirmed with a recovery ranging from 93.2 to 99.3%. The strategy of combining AuNPs/N-GQDs-P-MOF and glucose oxidase for cascade catalysis in this work provides a simple yet efficient approach to prepare nanomaterial-based enzyme-mimics and enzymes hybrids with high performance, which holds great potential in biological and chemical applications of electrochemical devices and biosensing.
研究领域:
生物传感器技术
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电化学分析
·
纳米材料科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Dongyan Li,Huaiyue Xia,Yingying Sun,Wenjie Liu,Wen Liu,Jianna Yu,Guoxing Jing,Jian Zhang,Wenshan Li
Ochratoxin A(OTA), a highly toxic mycotoxin commonly found in food, poses a serious threat to health even at low concentrations. Developing a sensitive, accurate, simple, and cost-effective detection method is of great significance for food safety and quality control. Herein, a triple cascade amplification strategy was used to construct the colorimetric assay for the detection of OTA, where the amplification process consists of an entropy-driven DNA circuit (EDC), a catalytic hairpin assembly (CHA), and Mg 2+ -assisted DNAzyme catalysis (MNAzyme). Through the specific binding of ochratoxin A (OTA) and its aptamer , an initiator strand is released to initiate upstream EDC and then produce a new trigger unit that motivates downstream CHA to generate MNAzyme, which further cleaves the substrate strand to induce the formation of G-quadruplex/hemin DNAzyme as a signal readout. The aptasensor was shown to detect OTA, with a low detection limit of 8.7 fM and good selectivity . The developed method could be used as a highly colorimetric aptasensor for the detection of OTA in spiked rice samples.
研究领域:
食品安全
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Qingyou Cai,Fanfan Wang,Jingying Ge,Zhiguo Xu,Mei Li,Hui Xu,Hua Wang
Herein, we reported the G-wire-based self-quenched fluorescence probe and its application in ultrasensitive microRNA (miRNA) detection by combining with target-activated isothermal cascade amplification. The terminal-single-fluorescein (FAM)-labeled G-rich oligonucletides self-assembled into G-wire nanostructures (G-wires) with K + and Mg 2+ . Thereafter, the G-wires brought terminal-labeled FAM into close proximity, as a result, the self-quenched signal probe formed. Besides, when there was the target miRNA, target-activated isothermal cascade amplification converted miRNA into the copious trigger DNA. After hybridization between trigger DNA and the self-quenched probe, the G-wires were splited and forced the apart of proximate FAM, and then the self-quenched probe displayed an “on” mechanism. Therefore, the approach gave a limit of detection (LOM) of 0.82 aM to miRNA-21 and could be implemented within a wide linear range of 2 aM to 2 nM. This approach was able to distinguish the single-mismatched miRNA-21, which was selective and sensitive in detecting human spiked serum samples.
研究领域:
分子生物学
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生物化学
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纳米技术
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癌症诊断
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Yanping He,Peixuan Li,Xin Zhao,Dongmei Wang,Fuqiang Zhang
The abuse or misuse of fleroxacin (FLE) in aquaculture and livestock may elevate FLE residues in foods, posing a serious threat to human health. Consequently, FLE detection has garnered extensive attention in food science. This study developed a magnetic separation-assisted colorimetric aptasensor for ultrasensitive FLE detection, leveraging the enhanced nanozyme activity of CoOOH nanoflakes with hairpins and dual cascade amplification (CHA-HCR). Under optimal experimental conditions, the proposed aptasensor exhibited high specificity for FLE with a wide linear range (1–100 nM) and a detection limit as low as 758 pM. Furthermore, the colorimetric aptasensor demonstrated robust performance in FLE-spiked honey analysis, achieving 96.70–101.06 % recoveries with 2.42–5.45 % RSDs, which showed strong agreement with commercial ELISA results. This study establishes a simple and effective platform for sensitive FLE detection in honey samples, demonstrating significant potential for practical food safety monitoring.
研究领域:
食品科学
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分析化学
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纳米技术
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环境卫生
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生物传感器
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 13
·2009
·
IF 4.7
Guifang Xie,Fujing Mao,Yirui Huang,Li Wen,Zhichang Sun,Zhenyun He,Xing Liu
Deoxynivalenol (DON) is a common mycotoxin that contaminates cereals. Therefore, the development of sensitive and efficient detection methods for DON is essential to guarantee food safety and human health. In this study, an enzyme cascade amplification-based immunoassay (ECAIA) using a dual-functional alkaline phosphatase-linked single-chain fragment variable fusion tracer (scFv-ALP) and MnO2nanosheets was established for DON detection. The scFv-ALP effectively catalyzes the hydrolysis of ascorbyl-2-phosphate (AAP) to produce ascorbic acid (AA). This AA subsequently interacts with MnO2nanosheets to initiate a redox reaction that results in the loss of oxidizing properties of MnO2. In the absence of ALP, MnO2nanosheets can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce the blue oxidized product of TMB, which exhibits a signal at a wavelength of 650 nm for quantitative analysis. After optimization, the ECAIA had a limit of detection of 0.45 ng/mL and a linear range of 1.2–35.41 ng/mL. The ECAIA exhibited good accuracy in recovery experiments and high selectivity for DON. Moreover, the detection results of the actual corn samples correlated well with those from high-performance liquid chromatography. Overall, the proposed ECAIA based on the scFv-ALP and MnO2nanosheets was demonstrated as a reliable tool for the detection of DON in corn samples.
研究领域:
食品安全
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiaqi Xue,Kang Mao,Zihui Tang,Jiming Hu,Hua Zhang
Owing to the severe environmental and health issues posed by organophosphorus pesticides (OPs), a dual-enzyme cascade biosensing platform based on manganese dioxide (MnO2) and CRISPR/Cas12a was developed in this study. Smartphones were innovatively integrated with a stacked ensemble learning (SEL) model for ultrasensitive detection and dynamic monitoring of OPs in environmental water samples. A dual-enzyme cascade signal amplification strategy was used to construct this sensing platform. Acetylcholinesterase (AChE) catalyzes the generation of thiocholine (TCh), which, in turn, regulates the degradation of MnO2 nanosheets, releasing Cas12a activators and generating a fluorescence signal. Owing to the irreversible inhibition of AChE activity by OPs, dichlorvos (DDVP) was successfully detected, with a detection limit as low as 4.62 pg/mL. Additionally, the SEL model, integrated into the smartphone biosensing platform and incorporating random forest (RF), XGBoost, and ridge regression algorithms, exhibited strong performance in detecting OPs after optimization (R2 = 0.9985). In real water samples, the SEL model achieved a recovery rate of 93.1–103.1%, and the degradation kinetics of DDVP were successfully monitored over 24 h, revealing significant differences in DDVP degradation rates across various water matrices. This study is the first to report the integration of CRISPR/Cas12a biosensing technology with an SEL model-driven smartphone detection platform, providing a novel approach for sensitive, portable, and intelligent monitoring of OPs and offering new insights for water quality monitoring and early detection of environmental risks.
研究领域:
环境监测
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分析化学
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生物传感
·
分子生物学
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环境毒理学
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水污染控制
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Mengran Wang,Linqing Shan,Xianglong Kong,Ruiyan Pan,Haiwei Wang,Jin Zhou,Jingjing Ming
Monitoring the information of aflatoxin M1 (AFM1) is of great significance for food safety since its widespread presence in milk and other dairy products. In view of this, we developed a label-free fluorescence DNA walker based on self-protected DNAzyme for determination of AFM1. By inserting the AFM1 aptamer into DNAzyme, the complete DNAzyme was split into two separated parts and designed to be an arch-like shield to avoid its degradation in complex matrix. This excellent structure of DNAzyme and the report probe were pre-assembled on the surface of gold nanoparticles (AuNPs). Through the high efficiency affinity between AFM1 and its aptamer, the DNAzyme could be activated and show its high catalytic cleavage towards its substrate of report probe. The releasing G-quardruplex (G4) of this report probe from AuNPs could combine with berberine and exhibit obvious fluorescence values. Under optimized experimental conditions, the AFM1 detection limit was achieved at 6.40 ng/L. This DNA walker exhibited good selectivity, reproductivity and stability. Furthermore, the feasibility for analysis in real sample of our method was performed towards the target spiked in milk, indicating its potential application for real sample determination.
研究领域:
分析化学
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食品科学
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纳米技术
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生物技术
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产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Yujia Liang,Zhengmin Cai,Yamei Tang,Chenglin Su,Liye Xie,Yan Li,Xinqiang Liang
Introduction: Reactive oxygen species (ROS)-mediated therapies have typically been considered as noninvasive tumor treatments owing to their high selectivity and efficiency. However, the harsh tumor microenvironment severely impairs their efficiency. Methods: Herein, the biodegradable Cu-doped zeolitic imidazolate framework-8 (ZIF-8) was synthesized for loading photosensitizer Chlorin e6 (Ce6) and CaO 2 nanoparticles, followed by surface decoration by hyaluronic acid (HA), obtaining HA/CaO 2 -Ce6@Cu-ZIF nano platform. Results and Discussion: Once HA/CaO 2 -Ce6@Cu-ZIF targets tumor sites, the degradation of Ce6 and CaO 2 release from the HA/CaO 2 -Ce6@Cu-ZIF in response to the acid environment, while the Cu 2+ active sites on Cu-ZIF are exposed. The released CaO 2 decompose to generate hydrogen peroxide (H 2 O 2 ) and oxygen (O 2 ), which alleviate the insufficiency of intracellular H 2 O 2 and hypoxia in tumor microenvironment (TME), effectively enhancing the production of hydroxyl radical (•OH) and singlet oxygen ( 1 O 2 ) in Cu 2+ -mediated chemodynamic therapy (CDT) and Ce6-induced photodynamic therapy (PDT), respectively. Importantly, Ca 2+ originating from CaO 2 could further enhance oxidative stress and result in mitochondrial dysfunction induced by Ca 2+ overloading. Conclusion: Thus, the H 2 O 2 /O 2 self-supplying and Ca 2+ overloading ZIF-based nanoplatform for cascade-amplified CDT/PDT synergistic strategy is promising for highly efficient anticancer therapy.
研究领域:
纳米技术
·
癌症治疗
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 35
·21760-21766
·
IF 3.361
Zeling Zhang,Benchao Su,Huan Xu,Zhenyun He,Yuling Zhou,Qi Chen,Zhichang Sun,Hongmei Cao,Xing Liu
Ochratoxin A (OTA) is a common food contaminant with multiple toxicities and thus rapid and accurate detection of OTA is indispensable to minimize the threat of OTA to public health. Herein a novel enzyme cascade-amplified immunoassay (ECAIA) based on the mutated nanobody–alkaline phosphatase fusion (mNb–AP) and MnO2 nanosheets was established for detecting OTA in coffee. The detection principle is that the dual functional mNb–AP could specifically recognize OTA and dephosphorylate the ascorbic acid-2-phosphate (AAP) into ascorbic acid (AA), and the MnO2 nanosheets mimicking the oxidase could be reduced by AA into Mn2+ and catalyze the 3,3′,5,5′-tetramethyl benzidine into blue oxidized product for quantification. Using the optimal conditions, the ECAIA could be finished within 132.5 min and shows a limit of detection of 3.38 ng mL−1 (IC10) with an IC50 of 7.65 ng mL−1 and a linear range (IC20–IC80) of 4.55–12.85 ng mL−1. The ECAIA is highly selective for OTA. Good recovery rates (84.3–113%) with a relative standard deviation of 1.3–3% were obtained and confirmed by high performance liquid chromatography with a fluorescence detector. The developed ECAIA was demonstrated to be a useful tool for the detection of OTA in coffee which provides a reference for the analysis of other toxic small molecules.
研究领域:
食品科学
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分析化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 6
·1585-1595
·
IF 3.641
Chen Ben-Qi,Yang Meng,Liao Wen-Chun,Li Zi-Ying,Chen Zhi-Xiong,Yang Yun-Hui,Yang Tong,Hu Rong
Developing a strategy for the determination of trace ochratoxin A (OTA) in food is very important to ensure food safety. Here, based on a tripod probe, an electrochemical aptasensing platform for detected OTA sensitively was designed, which used the 3D DNA walker by endonuclease Nb.BbvCI
powered and the nanozyme of porous Co3O4 nanodisks loaded Pt nanoparticles (Pt NPs@Co3O4) as a cascaded signal amplifying device. With OTA as a model target, the swing-arm probe of DNA walker was triggered. Next, probe on the magnetic beads was cleaved
and released by Nb.BbvCI to realize the first signal amplification. After that, the partial sequence of the released probe is well assembled with triple-strand DNA by strand reaction and the other sequence exposed as a capture probe was combined with the Pt NPs@Co3O4.
It can efficiently make the decomposition of H2O2, bring about significant current, and the signal was amplified again. The designed electrochemical aptasensor demonstrated outstanding detection linearity in the OTA concentration range of 1 pg/mL to 100 ng/mL, with a
detection limit of 0.194 pg/mL (S/N = 3), with excellent reproducibility and high specificity. It can be used to find OTA in food. This research supplies a potential split-new avenue for the sensitive detection of electrochemical aptasensor.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
BME Frontiers
·
2025
·
IF 7.7
Yirui Zhang,Jieyu Yan,Wangheng Hou,Qian Gao,Tao Zhang,Yan Gao,Jingwen Li,Kun Han
Objective: Accurate and rapid detection of disease biomarkers is critical for early diagnosis, timely intervention, and effective disease management. Impact Statement: This strategy is exemplified through the development of 2 biosensors for detecting HIV-1 DNA and HIV-1 p24, biomarkers associated with acquired immunodeficiency syndrome (AIDS). Introduction: We propose a general biosensing strategy that leverages a treble signal amplification cascade, demonstrating its versatility and applicability across diverse biomolecular targets. Methods: The method integrates multiple amplification mechanisms to achieve unparalleled sensitivity. Initially, the hybridization of 2 aided probes with the target triggers isothermal amplification facilitated by polymerase and nicking enzyme, providing a robust preliminary signal enhancement. Repeated cycles of primer extension, nicking, and signal primer dissociation then generate multiple signal primers. These primers are further amplified via rolling circle amplification (RCA), resulting in an important secondary signal boost. Finally, the amplified products activate CRISPR-Cas12a-mediated trans-cleavage, achieving a tertiary level of signal enhancement. Results: This cascade amplification approach achieves remarkable sensitivity, with detection limits of 62 aM for nucleic acids and 8.48 pg/ml for proteins, positioning it as a broadly applicable framework. Clinical samples were assayed, which indicates its capability in clinical diagnosis. Conclusion: Beyond HIV detection, the modular design of this strategy allows adaptation for various biomarkers, showcasing its potential as a universal platform for molecular diagnostics in healthcare and research.
研究领域:
生物传感器
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分子诊断
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病毒学
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临床诊断
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生物医学工程
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核酸扩增
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蛋白质检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）