NEW JOURNAL OF CHEMISTRY
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2023
·Vol. 47
·期 16
·7588-7594
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IF 3.3
Jianfeng Yan,Quanbin Fu,Shikai Zhang,Xianbao Shi,Yuanhong Zhang,Juying Hou,Junling Duan,Shiyun Ai
As a kind of organic basic compound containing nitrogen, biogenic amine is released from food spoilage and considered as an indicator for monitoring food freshness. Herein, an excellent dual-emission ratiometric fluorescent sensor for eight kinds of biogenic amines was established via the facile mixing of pH-responsive carbon dots and Rhodamine B. The developed sensor shows a rapid response (10 s), low limit of detection (0.733–3.943 μM) and a wide linear range (0–150 μM). More importantly, a portable fluorescent filter paper was prepared by simple dispersing the filter paper in a solution mixture of carbon dots and Rhodamine B. The obtained fluorescent filter paper based sensor showed an obvious color change in the presence of biogenic amine vapor derived from shrimp and mushroom. The results revealed that this sensor could realize the on-site and visual detection of biogenic amines in real food samples, making it suitable for potential application in food safety monitoring.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-13
·
IF 4.6
Xu Rui-Qi,Ma Ling,Chen Timson,Zhang Wei-Xiong,Chang Kuan,Wang Jing
Biosurfactants are attracting much interest due to their potential application as therapeutic agents in the medical and cosmetic field. Previous studies have demonstrated that biosurfactant such as sophorolipid (SL) exhibits immunomodulatory effects. In this article, we found the potential of sophorolipid for inhibiting histamine-induced itch and preliminarily explored its molecular basis. First, behavioral tests indicated that SL can remit the histamine-induced scratching behaviors of mice. Second, SL can suppress the the calcium influx induced by histamine, HTMT and VUF8430 in HaCaT cells. RT-PCR analysis showed that the histamine-induced upregulation of mRNA levels of phospholipase Cγ1, 1,4,5-trisphosphate receptor (IP3R), and protein kinase Cα can be inhibted by SL, suggesting that SL may impede the PLC/IP3R signaling pathway activated by histamine. In further tests, the capsaicin-induced calcium influx can also be inhibited by SL. The immunofluorescence and molecular docking analysis indicated that SL acts as an inhibitor of transient receptor potential vanilloid-1 (TRPV1) activation to decrease calcium influx against stimuli. In summary, these results revealed that SL may inhibit histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity. This paper indicates that SL can be a useful treatment for histamine-dependent itch.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Nuo Duan,Kexin Ren,Mingqian Song,Xinyue Zhang,Zhouping Wang,Fei Jia,Shijia Wu
The presence of biogenic amines (BAs) in food is not only important from a toxicological view, but can also be used as an indicator of food spoilage. However, the design of biosensors for the detection of BAs is very limited. Hence, in this work, a dual-donor fluorescence resonance energy transfer (FRET) aptasensor was established for simultaneous determination of histamine (HIS) and tyramine (TYR) using dual-color quantum dots (QDs) as energy donors and two-dimensional molybdenum sulfide nanosheets (MoS 2 NSs) as energy acceptor. Specific aptamer for HIS (Apt 1) and TYR (Apt 2) were labeled with two fluorescence emission QDs (QD 530 and QD 625 ), respectively. On the basis of the excellent adsorption ability of MoS 2 NSs toward aptamer, the fluorescence quenching can be observed between MoS 2 NSs and QD 530 -Apt1 and QD 625 -Apt2. Upon the addition of HIS and TYR, the aptamer conformation switches induced by the target binding mitigate the interaction force of MoS 2 NSs and QD 530 -Apt1 and QD 625 -Apt2, resulting in the fluorescence recovery. The method displays good sensitivity with limit of detection (LOD) of 0.1 ng/mL for HIS and 0.25 ng/mL for TYR, respectively. It can also be applied to the HIS and TYR detection in spiked fish samples with recovery of 95.2%∼106.6%, demonstrating its prospect in the sensitive detection of BAs in animal-derived foodstuff.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Qi Nie,Jingjing Deng,Bing Xie,Tianshu Zhou
In this study, a novel solvent-assisted strategy was developed for the rapid and sensitive detection of kanamycin (KAN) based on glutathione-capped silver-doped gold nanoclusters (AuAg NCs). Ethanol solvent played a key role in disrupting the hydration shell of metal(I)-thiolate complexes in AuAg NCs. KAN, as a specific linker between AuAg NCs through electrostatic interaction and metal-amine coordination, induced the formation of large aggregates and thus triggered prolonged photoluminescence lifetime as well as strong luminescence enhancement by restricting molecular motions in AuAg NCs. The KAN-stimulated turn-on principle allows AuAg NCs to exhibit 4-fold sensitivity in 40% ethanol/water (V/V) mixed solution compared to that in water. The improved aggregation-induced-emission enhancement (AIEE) feature endows the AuAg NCs with selective recognition for KAN and realizes rapid detection in a short response time of 60 s with a low detection limit of 8.6 nM. By integrating AuAg NCs with a Pasteur pipette, a facile sample-to-answer test kit for on-site determination with low cost and high efficiency was constructed, offering potential applications in the timely analysis of contaminants in environmental emergencies.
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JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Xiaoqi Lu,Siyu Ji,Zhengbing Ren,Shangkun Jiang,Qian Yu,Jiaqi Guo,Alan X. Wang,Xianming Kong
Food poisoning caused by histamine ingestion is one of the prevalent allergies associated with fish consumption in the world. Reliable detection of histamine from fish by a portable platform was of urgent importance to food safety. A portable technology for on-site monitoring of histamine in tuna was established through combined azo-derivatized thin-layer chromatography (TLC) with surface-enhanced Raman scattering (SERS) spectroscopy. The real tuna meat sample was directly applied onto the portable sensor for the separation of histamine and azo-derivatizing of histamine was reacted on the TLC plate. The colorless histamine was visualized by azo-derivatization after spraying Pauly reagent onto the diatomite TLC plate. The molecule information and concentration of the histamine was measured and calculated by SERS spectra. Diatomite TLC plate was capable of separating histamine with 1.32 × 10 −7 M of Au colloid for the SERS enhancement. Accordingly, the limit of detection of histamine from mixture sample could achieve 2.8 × 10 −4 ppm. These results indicated that the portable azo-derivatized TLC-SERS sensor not only visualizes the histamine but also improves the intensity of the Raman spectra. The azo-derivatized TLC-SERS sensor could be applied for rapid, convenient, and ultrasensitive point-of-care sensing of histamine in fish.
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JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Xin Huang,Rui Wang,Yikai Wang,Changbao Chen,Shuying Liu
Ethnopharmacological relevance The properties are the body's response to traditional Chinese medicine (TCM). The essence of traditional Chinese medicine properties are cold, hot, warm, and cool. In the theory of traditional Chinese medicine, ginseng is warm and American ginseng is cool, they present two opposite properties. The material basis of property differences and effect mechanism of property degree need further investigation. Aim of the study The aim of this work was to screen out the neurochemicals related to warm and cool properties of ginseng and American ginseng, and investigate the distributions of identified neurochemicals in rat brain and the metabolic mechanism. Materials and methods Spatial metabolomics was applied to study the effects of ginseng and American ginseng on the distributions of neurochemicals in rat brain by desorption electrospray ionization mass spectrometry imaging (DESI-MSI). Based on discriminant coefficients in partial least square discriminant analysis (PLS-DA) processing, neurochemicals related to warm and cool properties were classified. In addition, the score contributions of the neurochemicals markers could be used to evaluate the warm and cool property degrees. Results A total of 25 neurochemicals were imaged and identified in brain section. The distribution regions of main neurochemicals were consistent with in sagittal and coronal sections of brain reported in literature. 17 neurochemicals were classified as warm markers. Meanwhile, 8 neurochemicals were identified as cool markers, correlated with the cool properties of American ginseng. It demonstrated that the score contributions of the 25 neurochemicals markers could be used to evaluate the warm and cool property degrees. Based on the regulatory effects of neurochemicals, the warm markers could promote the body's energy metabolism, improve the function of endocrine system, and enhance the excitability of central nervous system. The cool property markers have reduced excitability of central nervous system, weakened metabolism and stress response ability, thus presented the biological activity of cool and cold. Conclusions Our findings provided a rapid and effective visualization method for the spatial distribution and metabolism of small molecular neurochemicals in rat brain. DESI-MSI was a reference methodology for evaluating the properties of TCM.
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Biomass Conversion and Biorefinery
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2023
·
IF 4
Si Lin,Hou Juying,Lv Wei,Hou Qin,Xu Zhixiang,Ai Shiyun
In this paper, lignocellulose components were separated under mild conditions to achieve hierarchical utilization of biomass. Cellulose was retained in cellulose pulp with highly crystalline structure, and lignin powder was recovered, which offered a possibility for the high-value utilization of lignocellulosic biomass. The extracted lignin (EL) was carbonized and activated to prepare lignin-based porous carbon microspheres (LPCMs) and further used as a substrate to interact with fluorescein-5-isothiocyanate (5-FITC) for the detection of biogenic amines (BAs). The fluorescence of 5-FITC was found to be quenched in the presence of LPCMs. And then, upon addition of BAs, 5-FITC was released from the surface of LPCMs, which resulted in the fluorescence recovery of 5-FITC. Thus, a novel “turn off–on” fluorescent probe was constructed for sensitive and visual detection of BAs. Meanwhile, the retained cellulose skeleton structure was used to prepare a smart identification label for real-time and visual evaluation of the freshness of pork and shrimp. This work realized the improvement of comprehensive utilization rate of lignocellulose and developed a rapid, efficient, and sustainable method which combined natural lignocellulose materials with green analytical technology for visually evaluating food quality.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 415
·期 18
·4265-4275
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IF 4.478
Wang Tingting,Chen Yihui,Hou Chunyan,Qiao Xiaoqiang
A novel type of PEG-modified halloysite was prepared and used as a hydrophilic interaction and cation exchange mixed-mode sorbent for solid-phase extraction of biogenic amines in fish samples. The eluates were analyzed by high-performance liquid chromatography-ultraviolet detection after the derivatization with benzoyl chloride. The developed sorbent was characterized by scanning electron microscopy, infrared spectroscopy, X-ray diffraction, zeta potential analyzer, and thermo-gravimetric analysis. After the optimization of various parameters influencing the extraction efficiency, the PEG-modified halloysite-based SPE method was evaluated. The adsorption capacities of putrescine, spermine, phenethylamine, and histamine were as high as 9.3, 8.5, 5.7, and 5.6 mg g −1 , respectively. Satisfactory reproducibility of sorbent preparation was obtained with within-batch and batch-to-batch relative standard deviations (RSDs) lower than 3.9% and 8.6%, respectively. The biogenic amine spiking recoveries in fish samples ranged from 84.3 to 105.5% with good RSDs lower than 7.8%. Intra-day and inter-day precision, expressed as RSDs, were better than 8.8%. The limits of detection of histamine, putrescine, phenethylamine, and spermine were 9.4, 1.9, 0.5, and 0.9 μg L −1 , respectively. This work provides a new hydrophilic interaction and cation exchange mixed-mode sorbent and is successfully applied to the extraction of trace biogenic amines from fish samples. Graphical Abstract
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TALANTA
·
2022
·
IF 6.556
Bin Zhou,Runlin Yang,Muhammad Sohail,Xiaoxue Kong,Xing Zhang,Ninghua Fu,Bingzhi Li
CRISPR is reshaping biosensing technology due to its programmability, sensitivity, and specificity. Most current CRISPR-based biosensors are developed based on Cas12 and Cas13, while the biosensing potentials of the newly discovered Cas14 have not been fully elucidated yet. Herein, a fluorometric biosensor named HARRY ( h ighly sensitive a ptamer- r egulated Cas14 R -loop for bioanal y sis) was developed. The diblock ssDNA is designed to contain the activator sequence of Cas14 and the aptamer sequence of specific targets. In the absence of targets, the ssDNA activates Cas14a, then the Cas14a trans -cleavages the fluorescent reporter, causing fluorescence enhancement. In the presence of the targets, ssDNA-target assembly is formed via aptamer interaction, resulting in the inhibition of Cas14a activation. HARRY can detect ATP , Cd 2+ , histamine, aflatoxin B1 , and thrombin with detection limits at the low-nanomolar level, which shows improvement compared with Cas12a-based aptasensors in sensitivity and versatility. We reasoned that the improvement is derived from the ssDNA specificity of Cas14a and found that the detection limit of HARRY is correlated to the binding affinities of aptamers. This study unlocks the potential of Cas14a in versatile aptasensing, which may inspire the development of CRISPR-based biosensors from the Cas14a branch.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiang Gao,Peng Nie,Peiran Li,Zhi Zheng,Jieshun Cheng,Ying Gu,Yizhong Shen
To combat food freshness fraud, it is urgent to develop a method which could realize the detection of biogenic amines (BAs) present in food. In our study, we developed a colorimetric and ratiometric fluorescence dual-mode sensor which integrated with silver metallization-based response system of AIE liposome + OPD + RSM + Ag + toward BAs in foods for fighting freshness fraud. With the hydrolysis from the alkaline of BAs to resorcinol monoacetate (RSM), the production resorcinol (RS) could metallize silver ion (Ag + ) to silver atoms (Ag 0 ) which could lead to a BAs concentration-dependent decrease of the oxidation product 2,3-diaminophenothiazine (DAP) of Ag + to o -phenylenediamine (OPD). As a result, the dual-mode sensor has a low detection limit and wide linear range in the spiked detection of soy products, pork and milk samples for BAs. Thus, providing a reliable method for food safety and forestalling food freshness fraud.
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DYES AND PIGMENTS
·
2023
·
IF 4.5
Zekun Shi,Yongning Wu,Xue Zhang,Yanyan Gao,Luqin Qiao,Qin Hou,Minglin Wang
Fluorescent cuprous iodide (CuI)-based coordination polymers (CuI-CPs) were widely used in the sensor field for high selectivity and sensitivity. Here, a new CuI-CP fluorescent probe was fabricated for the detection of spermine, spermidine and cysteine with dual-mode, which was capable of both convenient colorimetric analysis and ratiometric fluorescent detection. The fluorescence of CuI-CP solution was quenched and the solution changed from white to purple upon addition of spermine or spermidine, while the solution changed from white to yellow for cysteine. This probe exhibited selective, sensitive, rapid and linear response to spermine, spermidine and cysteine with a limit of detection of 0.57, 1.06 and 1.18 μM, respectively. Furthermore, cellulose acetate was used as the matrix to fabricate intelligent fluorescent film for real-time and visual monitoring the freshness of pork samples based on distinct fluorescence changes under UV light. This work realized a rapid, efficient, and profitable method for visually evaluating food quality.
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Jing Han,Xinping Lin,Huipeng Liang,Sufang Zhang,Beiwei Zhu,Chaofan Ji
Biogenic amines (BA) are often present at high concentrations in fermented meat and cause foodborne illness. The aim of this work was to screen amine-degrading starters for Roucha (a fermented meat) to improve product safety and quality. Firstly, Weissella viridescens F2 and Lactiplantibacillus plantarum His6 with multi-copper oxidase activity and high degradation rates towards histamine and tyramine were selected as single or mixed starters. Additionally, the effect of starters on bacterial community succession, acid production, BA accumulation, free amino acid profiles, and volatile compound profiles were evaluated during the fermentation of Roucha. Results indicated that all starters could effectively reduce the accumulation of BA in Roucha. At the end of fermentation, Lpb. plantarum His6 as a single starter reduced the histamine level (38.15 %), while mixed starters ( Lpb. plantarum His6 + W. viridescens F2) possessed a high tyramine-reduction rate (54.95 %) and total BA-reduction rate (46.64 %). Bacterial succession analysis revealed that starters could help establish the dominance of lactobacilli or Weissella quickly, which contributed to the direct degradation of BA and inhibited the growth of amine-producing bacteria. Furthermore, Lpb. plantarum His6 could promote the accumulation of essential amino acids (leucine, phenylalanine, threonine, isoleucine), sweet and umami amino acids (glycine, threonine, glutamate), and volatile compounds of good odor, indicating that this strain greatly contributed to the development of good taste and aroma characteristics in Roucha. Overall, this work shows that the application of amine-degrading starters is an effective strategy to reduce the BA accumulation and improve the quality of Roucha. Thus, these findings can provide foundations for the industrial production of Roucha.
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