EUROPEAN JOURNAL OF PHARMACOLOGY
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2020
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IF 3.263
Yanzheng Xie,Lei Xie,Ailan Chen,Shengnan Wu,Yuxiao Mo,Xiaoqiang Guo,Changchun Zeng,Xinan Huang,Jinyang He
AIDS , a serious fatal disease caused by the human immunodeficiency virus (HIV), is an epidemic disease for which no effective vaccine has been established. The current therapeutic interventions for AIDS have limited efficacy because they are unable to clear HIV infections and the continuous occurrence of resistant HIV strains. Therefore, the exploitation of new drugs to prevent the spread of AIDS remains a high priority. In this study, the effects of icariin and its metabolite anhydroicaritin on SIV/HIV replication were investigated. In CEM × 174 cells and PBMC cells, both icariin and anhydroicaritin can significantly inhibit HIV-1 or SIVmac251 replication. Furthermore, molecular docking studies revealed that icariin and anhydroicaritin can act on both HIV reverse transcriptase and protease but could not bind to integrase . Reverse transcriptase and protease inhibition biological assays showed that both icariin and anhydroicaritin could significantly inhibit only HIV reverse transcriptase. In summary, the two compounds can significantly inhibit HIV/SIV in vitro and their targets may be mainly involved with HIV reverse transcriptase.
研究领域:
病毒学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Tianle Wu,Peng Wang,Yuyu Zhang,Ping Zhan,Yu Zhao,Honglei Tian,Wanying He
Mutton possesses a typical flavor, known as “muttony” or “goaty”, which significantly limits consumers’ acceptability and its further popularization. Generally, this unpleasant flavor originates from mutton tallow. Thus, we first characterized the key volatiles of the cooked mutton tallow (CMT) via gas chromatography-mass spectrometry/olfactometry (GC–MS/O) and odor activity value (OAV). Combined with aroma recombination and omission tests, eleven compounds, involving 4-methyloctanoic acid, 4-methynonanoic acid, octanoic acid, decanoic acid, hexanal, heptanal, ( E )-2-octenal, ( E )-2-nonenal, ( E )-2-decenal, 2-nonanone and 2-penty-furan, were screened out to be responsible for the “muttony” flavor. The objective of this study was to investigate the sensory property and acceptability of CMTs, elaborated with 4 different levels of thyme phenolic extract (TPE), through descriptive sensory analysis and key muttony-related compounds identification. The results showed that, of different TPEs employed, CMT plus TPE3 was the most effective strategy to control the key “muttony” contributors, thereby to improve flavor profile of CMT.
研究领域:
食品科学
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风味化学
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感官评价
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Zhenhua Li,Xiaoqin Wang,Xiaoli Deng,Jiansen Song,Tong Yang,Yujie Liao,Guiping Gong,Linjuan Huang,Yu Lu,Zhongfu Wang
Glycosphingolipids (GSLs) in human milk regulate the immune system, support intestinal maturation, and prevent gut pathogens. The structural complexity and low abundance of GSLs limits their systematic analysis. Here, we coupled the use of monosialoganglioside 1–2-amino-N-(2-aminoethyl) benzamide (GM1-AEAB) derivatives as internal standards with HILIC-MS/MS to qualitatively and quantitatively compare GSLs in human, bovine, and goat milk. One neutral glycosphingolipid (GB) and 33 gangliosides were found in human milk, of which 22 were newly detected and three were fucosylated. Five GB and 26 gangliosides were identified in bovine milk , of which 21 were newly discovered. Four GB and 33 gangliosides were detected in goat milk, 23 of them newly reported. GM1 was the main GSL in human milk; whereas disialoganglioside 3 (GD3) and monosialogangloside 3 (GM3) were dominant in bovine and goat milk, respectively; N -acetylneuraminic acid (Neu5Ac) was detected in >88 % of GSLs in bovine and goat milk. N -hydroxyacetylneuraminic acid (Neu5Gc)-modified GSLs were 3.5 times more abundant in goat than in bovine milk; whereas GSLs modified with both Neu5Ac and Neu5Gc were 3 times more abundant in bovine than in goat milk. Given the health benefits of different GSLs, these results will facilitate the development of custom-designed human milk-based infant formula.
研究领域:
营养学
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生物化学
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代谢组学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2018
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IF 5.158
Pan Zhang,Nan Zhang,Qiang Wang,Ping Wang,Jiugang Yuan,Jinsong Shen,Xuerong Fan
Chitosan, a natural biopolymer, is used as a multifunctional agent for modification of wool either through chemical crosslinking or physical coating. For the first time, wool fabric has been modified with chitosan through disulfide bond breaking and reforming reactions. The chitosan was thiolated and then grafted onto the reduced wool fibers through disulfide bonds. In order to understand the mechanism of the grafting of thiolated chitosan onto wool, glutathione was used as a model compound for wool in the research. The structures of thiolated chitosan reacted with glutathione and wool fabrics grafted with thiolated chitosan were investigated by FTIR, 13 CNMR, XPS, XRD, SEM. The dyeability, shrink-resistance and biocompatibility were also tested. The results suggested that glutathione reacted with thiolated chitosan and formed disulfide bond. The thiolated chitosan-grafted wool fabric had good shrink-resistance and dyeability. Hydrophilicity and antibacterial properties were also improved compared with untreated wool fabric. The results provide a novel approach for modification of wool through fiber-intrinsic groups like disulfide bonds.
研究领域:
材料科学
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纺织工程
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生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Electronic Materials
·
2024
·
IF 4.3
Xianxian Li,Xiaohe Song,Liran Zhang,Yi Huang,Yiting Tong,Yang Chen,Changhuan Zhang,Han Wu,Pibo Ma
The extraction of wool keratin protein (WK) from wool waste has garnered attention for its potential application in tissue engineering, drug delivery, and flexible pressure sensors, contributing to the efficient utilization of waste wool. Herein, we utilized biobased polylactic acid (PLA) as the polymer for electrospinning and incorporated carbon nanotubes (CNTs) as conductive additives in the electrospinning solution. WK, extracted from waste wool via a reduction method, exhibited excellent dispersibility and stability of carbon nanotubes in the spinning solution, enabling the preparation of a highly sensitive resistive flexible pressure sensor. The results demonstrate that the PLA/WK/CNTs @CNTs flexible pressure sensor exhibits a high sensitivity of 12.64 kPa–1 and a fast response/recovery time of 63 ms. Moreover, it possesses the lowest detection limit of 5.64 Pa, excellent stability over 6000 cycles, and good linearity within a wide working range (0–30 kPa), making it suitable for wearable biometric monitoring applications such as finger pressure, finger bending, and pulse monitoring, as well as visual interaction such as interactive blankets for infants. Therefore, we propose a high-quality wool waste utilization scheme, showcasing the potential application of discarded wool in smart living.
研究领域:
材料科学
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纳米技术
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生物医学工程
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可持续材料
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产品关联
DOI
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2019
·
IF 4.462
Yongqiang Zheng,Lingli Lu,Zhengli Yan,Sufang Jiang,Shanyi Yang,Yingzi Zhang,Kangwei Xu,Chunlian He,Xiaojun Tao,Qiufang Zhang
Icariin (ICA), a major active ingredient from Chinese medicine, has unique pharmacological effects on ischaemic heart disease. However, its hydrophobic property limits its administration and leads to poor efficacy. This work aimed to change its hydrophobic property and improve the treatment efficacy. We designed a new nano-drug to increase the ICA delivery. ICA was modified with hydrophilic polyethylene glycol monomethyl ether (mPEG) by a succinic anhydride linker to form a polyethylene glycol-icariin (mPEG-ICA) polymer. The structure of this polymer was identified by Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The content of ICA in the polymer was 32% as detected by ultraviolet spectrophotometry. mPEG-ICA nanoparticles, of 143.3 nm, were prepared by the dialysis method, and zeta potential was 0.439 mV by dynamic light scattering. The nanoparticles had a spherical shape on transmission electron microscopy. In media with pH 7.4 and 6.8, ICA release from mPEG-ICA nanoparticles after 72 h was about 0.78% and 64.05%, respectively, so the ICA release depended on the release media pH. On MTT and lactate dehydrogenase activity assay, mPEG-ICA nanoparticles could reduce cell damage induced by oxgen-glucose deprivation. Hoechst 33258 staining and TUNEL and AnnexinV-FITC/PI double staining showed that ICA nanoparticles could increase the activity of H9c2 cardiomyocytes under oxgen-glucose deprivation conditions by decreasing apoptosis. ICA modified by hydrophilic mPEG could improve its efficacy.
研究领域:
药理学
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纳米医学
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心脏病学
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Kai Jiang,Yiwei Huang,Rui Cui,Yuyue Qin,Thanapop Soteyome,Margaret Brennan,Charles Brennan
In this study, we screened out the dominant spoilage microorganisms during the cold storage of morels, prepared composite paper with a targeted selection of antimicrobial active substances, applied the composite paper to morels for cold storage and preservation, and investigated the changes of physicochemical indexes, flavor-presenting nucleotides and enzyme activities throughout the process of storage, and evaluated the freshness preservation effect. The pathogenic microorganisms during the natural spoilage of Morels were isolated and purified at 4 °C. The dominant spoilage microorganisms obtained were: bacterium B1 ( Lelliia amnigena ), fungi F2 ( Paecilomyces penicillatus ) and F3 ( Mucor hiemalis ). Inhibition experiments were carried out by selecting various active antimicrobial agents—lemon essential oil, clove essential oil and nisin (NI)—against the dominant spoilage microorganisms B1, F2 and F3. Comparing the inhibitory effects, NI was selected as an inhibitor for the dominant spoilage microorganisms following comprehensive consideration. The antimicrobial system (NI/C-CNC) was prepared by incorporating NI into carboxylated cellulose nanocrystals (C-CNC), and it exhibited a strong blocking effect on ultraviolet rays, which became more pronounced with the increase of NI addition. The composite paper NI-C was prepared using the NI/C-CNC antimicrobial system and absorbent paper (100 % virgin wood pulp), which showed better mechanical properties and reduced water vapor transmission rate (WVP). NI-C composite paper was applied to the preservation of morels, which effectively inhibited the dominant pathogenic bacteria. The flavor 5′-nucleotides were effectively protected, and it significantly slowed down the change in hardness during storage of morels. It also inhibited lipid peroxidation, as evidenced by the accumulation of malondialdehyde (MAD) content and reactive oxygen species (ROS), and had a positive effect on the control of enzyme activity. NI-C antimicrobial composite paper has good application prospects in the field of packaging of morels.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2022
·
IF 4.225
Shen Duan,Xin-Yao Wang,Xu Feng,Yue Ma,Zhong Zheng,Chao-Mei Ma
ABSTRACT Cynomorium songaricum is a traditional medicine and also a food material that is eaten raw or processed as tea or beverages. As a featured plant in semi-desert grasslands, C. songaricum is also eaten by the cattle and sheep in the area. This research study fed dairy sheep C. songaricum to determine the flavan-3-ols in sheep milk. Catechin (Cat), epicatechin (Epi), procyanidin A1 (A1), procyanidin A2 (A2), and procyanidin B1 (B1) were detected in sheep milk with the concentration being Epi > A2 > Cat > B1 > A1 at 24 h after the administration of C. songaricum . Neither A1 nor A2 were detected in the methanol extract of C. songaricum . Cysteine degradation of the plant revealed that in addition to Epi, A2 was the extending unit of the polymeric flavan-3-ols in C. songaricum , indicating that A2 is released digestively from the polymers and enters the milk. Procyanidin B-1 was converted to A1 on incubation in raw but not heated milk, indicating that the A1 in milk is the enzymatically transformed product of B1. Accelerated oxidation showed that the flavan-3-ols, B1, Cat, and Epi significantly protects the unsaturated triacyglycerols in the milk from oxidation. The flavan-3-ol could slow down the oxidation of glutathione and the latter may play an important role in preventing the milk triglycerides from oxidation. Flavan-3-ols are polyphenols with many health benefits. The present research revealed the antioxidant activities of flavan-3-ols that could be absorbed to sheep milk, adding new evidences for the values of these flavan-3-ols and for the milk.
研究领域:
食品科学
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动物营养学
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植物化学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Wei Jia,Zibian Fan,Qingyun Shi,Rong Zhang,Xin Wang,Lin Shi
The current study applied an untargeted metabolomics approach by ultra high performance liquid chromatography quadrupole-orbitaltrap high resolution mass spectrometry (UHPLC-Q-Oritrap-MS) to identify the chemical composition of irradiated goat meat and investigate the effect of irradiation on its metabolic profile and meat quality. A total of 103 metabolites were identified as differential metabolites responsible for metabolic changes in irradiated goat meat, which were involved in phenylalanine , tyrosine and tryptophan biosynthesis, phenylalanine metabolism and purine metabolism. Differential metabolites comprising amino acids , nucleotides and their derivatives were determined as the discriminating factors responsible for the meat quality during irradiation. Specifically, the levels of L-phenylalanine, L-isoleucine, L-histidine, guanosine, guanine, creatinine, glutathione and nicotinic acid were increased while inosine 5′-monophosphate (IMP) and guanosine 5′-monophosphate (GMP) were decreased. Overall, except for L-phenylalanine and guanine, other related metabolites significantly decreased with storage. This study contributes to a comprehensive understanding of the effect of irradiation doses and storage time on goat meat metabolism at the molecular level, so as to assess the quality of irradiated goat meat. Satisfactory results with linearity (R 2 > 0.995), precision (RSD less than 8.9%) and recovery (83%-106%) were obtained, demonstrating that the untargeted mebabolomics approach was appropriate for monitoring the changes of small molecular metabolites in irradiated goat meat and irradiation is a feasible method for goat meat preservation .
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 21
·13553
·
IF 6.208
Bo Li,Jiaying Li,Yanqin Shen,Hailiang Wu,Yanli Sun,Pengfei Zhang,Meihui Yang
The particular scale structure and mechanical properties of wool fiber make its associated fabrics prone to felting, seriously affecting the service life of wool products. Although the existing Chlorine–Hercosett treatment has a remarkable effect, it can lead to environmental pollution. Therefore, it is of great significance to develop an environmentally friendly and effective shrink-proof finishing technology. For this study, L-cysteine was mixed with protease to form a treatment solution system for shrink-proof finishing of wool fibers. The reduction performance of L-cysteine and its effect on wool were compared with those of other reagents, demonstrating that L-cysteine has an obvious reduction and destruction effect on the wool scale layer. Based on this, L-cysteine and protease 16L were mixed in a certain proportion to prepare an L-cysteine/protease treatment solution system (L/PTSS). The shrink-proof finishing of a wool top was carried out by the continuous multiple-padding method, and the processing parameters were optimized using the response surface method. The results indicated that when the concentrations of L-cysteine and protease 16L were 9 g/L and 1 g/L, respectively, the wool was padded five times at 50 °C, and each immersion time was 30 s, the felt ball density of the treated wool reduced from 135.86 kg/m3to 48.65 kg/m3. The structure and properties of the treated wool were also characterized using SEM, TG, and tensile strength tests, which indicated that the fiber scale structure was stripped evenly. Meanwhile, the treated fibers still retained adequate thermal and mechanical properties, indicating suitable application value. XPS, FT-IR, Raman, UV absorbance, and other test results revealed the reaction mechanism of L/PTSS with the wool fibers. After L-cysteine rapidly reduced the disulfide bonds in wool, protease can hydrolyze peptide chains more effectively, causing the scale layer to gradually peel off. Compared with the chlorination method and other protease shrink-proof technologies, L/PTSS can achieve the finishing effect on wool rapidly and effectively, without causing excessive pollution to the environment. The conclusions of this study provide a foundation for the development and industrial application of biological enzyme shrink-proof finishing technology.Keywords:wool;shrink-proof finishing;protease;L-cysteine
研究领域:
纺织科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Jiajun Pan,Zhaopeng Xia,Haibao Zhang,Liwei Wu,Yang Lu,Yong Liu,Yujie Cao
Animal fibres are coloured using dyes to satisfy the individual needs; thus, dark animal fibres must be bleached. Compared with frequently used Fe 2+ -catalysed H 2 O 2 (Fe 2+ /H 2 O 2 ) bleaching, persulphate (PDS)/H 2 O 2 bleaching is advantageous owing to its high efficiency, low damage of fibre and environmental friendliness. Herein, different free radical species were precisely controlled by changing the pH of the PDS/H 2 O 2 solution and regulating the existence of NH 4 + . When NH 4 + was absent, sulphate radicals (·SO 4 − ) and hydroxyl radicals (·HO) could be produced at pH = 8–9 and superoxide anions (·O 2 − ) could be produced at pH = 10. In the presence of NH 4 + , ·SO 4 − and ·HO could be produced at pH = 8, and ·O 2 − was dominant in the solution when pH = 9–10. Black yak wool was used as the research object to demonstrate that the bleaching ability of the generated ·O 2 − was stronger than those of the generated ·SO 4 − and ·HO. Compared with that of the commercial product (prepared using Fe 2+ /H 2 O 2 bleaching), the bleaching time of the decolourised yak wool was reduced by 83.33% (from 24 to 4 h) without the catalysis by heavy metal ions . Meanwhile, the whiteness of the decolourised yak wool (72.31%) was close to that of the commercial product (78.79%). Furthermore, the damage to the cuticle layer and cortex of yak wool was low after bleaching. The breaking strength of the decolourised yak wool was 1.79 cN·dtex −1 , which only decreased by 17.13% compared with that of raw yak wool. These results hold great potential in developing environmental-friendly bleaching methods for dark animal fibres with high efficiency and low damage.
研究领域:
纺织科学
·
环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jingjing Zhang,Jing Huang,Yufan Yang,Daxin Zhao,Cong Wang,Peng Zhong,Jianhua Jia,Weifan Dang,Qiwei Lu,Chengyu Zhang,Xiaohui Yan
Icaritin (ICT), a compound with diverse biological activities derived from Epimedium folium, is typically present in low concentrations in EFs. However, the abundant glycosyl-modified ICT compounds facilitate its transformation into ICT. Current biocatalytic production faces challenges, including low conversion rates and limited enzyme activity. This study developed a one-pot enzymatic cascade strategy for directly biotransform crude extracts of Epimedium folium (EEF) to produce ICT. The feasibility of catalyzing different ICT-related compounds in EEF was validated through molecular docking and substrate reactions. The selected glycosidase exhibited simultaneous activities as a glucosidase, xylosidase, and α-1,6-rhamnosidase, with the rhamnosidase showing outer-rhamnosidic activity and weak glucosidase activity. By using EFs as the substrate and employing whole-cells ( Escherichia coli ) containing LacS and BtRha proteins for synergistic catalysis, icariin can be efficiently synthesized within 6 h, achieving a conversion rate of 100 %. The enzymatic cascade for ICT production from crude extracts was elucidated by analyzing catalytic intermediates via HPLC. Compared to strategies using single or traditional multi-enzyme applications, this method shows advantages of ease to operation, high efficiency, and large production yield performance. This method has the potential to become an eco-friendly catalytic strategy for the large-scale production of icaritin.
研究领域:
生物技术
·
生物化学
·
生药学
·
酶学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）