SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.6
Lian-Lian Duan,Wen-Jun Liu,Rui Zhai,Zhen-Guang Wang,Hong-Yuan Yan,Yun-Kai Lv
This study employed a pore-confined synthesis strategy to achieve the in situ growth of bovine serum albumin-capped copper nanoclusters (BSA-CuNCs) within the UiO-66 framework (BSA-CuNCs@UiO-66). This nanocomposite enables highly sensitive and specific detection of creatinine (CR). Results demonstrated that the spatial confinement imposed by UiO-66 induced aggregation of the BSA-CuNCs and suppressed non-radiative transitions, leading to an approximately 10-fold enhancement in fluorescence intensity and a 11-fold increase in quantum yield. Leveraging the specific adsorption and enrichment capability of the UiO-66 framework toward CR, the BSA-CuNCs@UiO-66 fluorescence probe exhibited significant fluorescence quenching upon exposure to CR, achieving a detection range of 50–1000 nM and a detection limit of 30.81 nM. This work presented a novel confinement engineering strategy utilizing metal-organic frameworks (MOFs), establishing a new design paradigm for high-performance fluorescence probes with significant potential in bioanalytical applications.
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Nature Communications
·
2026
·
IF 15.7
Chen Yue,Chen Qinyi,Ma Yuanyuan,Zhang Cheng,Xu Jie,Li Kejing,Sun Yajie,Su Xinge,Wei Mingguang,Bao Rui,Ding Tong,Tang Longguang,Yu Wenying,Ju Shenghong,Fan Wenpei
Radiotherapy (RT) is a clinical mainstay of cancer treatment that triggers tumor-specific immune responses. However, the effectiveness is usually hampered due to the hypoxic tumor microenvironment (TME) and the ambivalent impact of RT on the immune landscape of tumors. Herein, we develop an injectable hydrogel encapsulating interleukin-12 (IL-12)/anti-CTLA-4 (aCTLA-4) co-engineered red blood cells (RBC), which is in situ self-assembled within the TME to increase oxygen supply and instigate sequential aCTLA-4/IL-12 release, thus achieving Ba/O 2 self-compensated radiosensitization and activating multistage immune responses. Once in the acidic TME, the in situ injected BaO 2 undergoes hydrolysis to generate H 2 O 2 and Ba 2+ , followed by the rapid reaction of Ba 2+ with sodium alginate to afford a biocompatible hydrogel. Meanwhile, catalase presented on RBC converts H 2 O 2 into O 2 , thereby alleviating hypoxia-induced radioresistance and inducing O 2 -mediated pore formation on RBC membrane for rapid release of aCTLA-4 to relieve tumor immunosuppression. Subsequently, IL-12 anchored on RBC is dilatorily released and interacts with T/NK cells within the TME to induce IFN-γ-dependent antitumor immunity. Taken together, the in situ self-assembled cell reservoir hydrogel offers a futuristic avenue to realize multistage radioimmunotherapy for effective tumor regression by programmable immunoregulation with significant clinical value.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
·
IF 5.6
Dechan Lu,Xiaoqi Zhou,Dandan Su,Jundong Li,Huizhen Lin,Rongmao Qiu
Abnormal serum creatinine levels are a key clinical biomarker for kidney dysfunction, making its accurate monitoring essential for early diagnosis and prevention. Current commercial kits based on the creatine kinase method are often affected by matrix interference, limiting their reliability in clinical settings. To address this issue, we developed a pH- assisted ratiometric surface-enhanced Raman scattering (SERS) approach for precise creatinine detection in biological samples. We synthesized core-shell nanoparticles (Au@MPA@Ag NPs) with 3-mercaptopropionic acid (MPA) as the internal standard. Under optimized alkaline conditions (pH = 9), the adsorption of creatinine onto the substrate was significantly enhanced, leading to a strong and reproducible SERS response. Moreover, the internal standard-calibrated Raman intensity exhibited a strong linear correlation with creatinine concentration, achieving the limit of detection as 3.3 μM. As a proof of concept, this method was successfully applied to quantify creatinine in human serum. The recovery rates ranged from 98% to 107%, demonstrating high consistency with results obtained using a commercial creatinine assay kit. This method provides a clinically accurate and reliable for creatinine monitoring, offering strong potential for point-of-care testing.
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MICROCHEMICAL JOURNAL
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2026
·
IF 5.2
Hongming Hou,Xiaoxue Liang,Xianglong Li
Efficient Cu 2+ -creatinine complexation is essential for the sensitivity of Cu-based non-enzymatic electrochemical creatinine sensors. Herein, a morphological optimization strategy for Cu nanostructures was applied to promote Cu 2+ -creatinine complexation. Accordingly, a screen-printed carbon electrode (SPCE) was pretreated with saturated sodium carbonate to form P-SPCE and subsequently subjected to one-step electrodeposition to fabricate a multi-morphological Cu-modified electrode (Cu/P-SPCE). The resulting multi-morphological Cu architecture enhances creatinine adsorption and facilitates the efficient formation of Cu 2+ -creatinine complexes, leading to improved detection sensitivity. Electrochemical testing revealed that the Cu/P-SPCE sensor achieved a wide linear range from 5 to 400 μM, a sensitivity of 5.01 μA·μM −1 ·cm −2 , and a detection limit of 2.86 μM. It showed promising selectivity for common interferences, such as uric acid and ascorbic acid, and excellent batch-to-batch reproducibility, with a relative standard deviation of 1.40% ( n = 12). Validation using human urine samples demonstrated good agreement with clinical enzyme assays, with accuracies exceeding 91.50%. These results indicate that morphological optimization of Cu-based electrodes provides an effective strategy for sensitive creatinine detection.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
·
IF 5.9
Yuheng Wang,Yumin Zhang,Yixiang Huang,Zixuan Xu,Min Li,Gaohong He,Xuehua Ruan,Wenjun Zhang
The catalytic activity of Ce-based nanozymes is significantly positive-correlated with the Ce 3 + ratio. In this work, Ce 3+ content in cerium oxyfluoride nanozymes was efficiently and directly regulated with fluorine-induced charge compensation between fluorine and oxygen, and stabilized by the electronegativity-driven inductive effect, through the facile and room-temperature synthesis for the enzyme /H 2 O 2 -free and highly sensitive UA detection. Remarkably, cerium oxyfluoride exhibited V max of 196 × 10 −8 M·s −1 and K m of 0.347 mM, indicating the superior oxidase-like activity. Thereby, ascribed to the antioxidant property of uric acid (UA), UA was effectively and promptly detected, i.e., a wide linear range of 0.3–1.2 mM, and ultra-low detection limit of 4.5 μM, as well as the significant anti-interference against the common urinary components. Prospectively, the electron-density reforming strategy through the strong electronegative fluorine introduction, extremely regulated the valence of cerium in cerium oxyfluoride to enhance the catalytic activity, via the synthetic route of mildness and facileness, fulfilling the effective real-time detection of UA.
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ACS Omega
·
2026
·
IF 5.2
Kermue Vasco Jarnda,Heng Dai,Huiting Hu,Yuan Tian,Richmond Anaman,Pian Wu,Ping Ding
Recent advancements in microfluidic paper-based analytical devices (μPADs) have demonstrated their significant potential for point-of-care testing (POCT), particularly in low-resource settings. These devices are especially promising for monitoring glucose levels in patients with diabetes, offering a cost-effective and portable solution. However, one of the primary challenges hindering the widespread adoption of smartphone-coupled μPADs has been their sensitivity to varying ambient lighting conditions, which can interfere with accurate colorimetric detection. To address this issue, researchers have introduced a novel approach involving a metal–organic framework (MOF), specifically Co-TCPP (Fe) [cobalt Fe(III) meso-tetra(4-carboxyphenyl) porphyrin chloride], synthesized using a straightforward method. Co-TCPP (Fe) exhibits remarkable peroxidase-like activity when exposed to hydrogen peroxide (H2O2), enabling it to interact effectively with classic chromogenic substrates and thus improving the colorimetric reaction crucial for accurate glucose detection. In this study, the team developed paper-based μPADs that incorporate the Co-TCPP (Fe) MOF, allowing it to accurately detect glucose levels across a wide concentration range. The limit of detection (LOD) achieved was 5.3 μM, with a linear detection range from 5 to 750 μM. This innovation addresses previous concerns regarding the impact of environmental lighting fluctuations and varying shooting angles, ensuring the reliability of glucose measurements under uncontrolled conditions. By integrating this algorithm into a smartphone-coupled μPAD system, the researchers have created a portable, high-resolution platform that delivers dependable results, even in challenging and fluctuating environments. This breakthrough marks a significant step toward enhancing the practicality and accessibility of glucose monitoring in resource-constrained settings, providing a valuable tool for both clinical and everyday use.
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Polymers
·
2026
·Vol. 18
·期 13
·1647
·
IF 5.8
Zhongqiu Ge,Hengfeng Zhu,Yiyang Chen,Yihang Rong,Zhuqun Shi,Quanling Yang
Most adsorbent materials typically face difficulties such as poor blood compatibility, weak mechanical strength, and high cost. In this study, oxidized 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) was used to obtain cellulose nanofiber (TOCN), and cellulose beads were prepared using a drop curing method. The structure, adsorption properties, and blood compatibility of the prepared beads were thoroughly investigated. The TOCN beads exhibit a uniform, nanometer-scale, three-dimensional porous structure. With increasing carboxyl content, after adsorption of TOCN beads, the bilirubin concentration in rabbit plasma decreased from 0.03 to 0.0089 mg mL−1within 90 min, which is significantly lower than the average bilirubin concentration in humans (about 0.01 mg mL−1), and the bilirubin concentration decreased by about 70%. The results illustrated the excellent blood compatibility, self-anticoagulant ability, and superior toxin removal capabilities of the TOCN beads, highlighting their potential as an ideal blood purification adsorbent.
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ANALYTICA CHIMICA ACTA
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2026
·
IF 6.1
Haiyan Cao,Bin Yang,Wenfei Dong,Wenbing Shi
Background The accurate detection of 2,3,5,6-tetramethylpyrazine (TMP) in complex matrices is significant for food quality control and biomedical analysis. Despite this need, a dedicated optical sensing platform for TMP has not been reported to date. Results Here, we present a novel ratiometric fluorescent probe based on a Tb–EDTA complex, which introduces a sensing strategy founded on coordination-induced modulation of the ligand-to-metal charge transfer (LMCT) process. Specific coordination of TMP's pyrazine nitrogen atoms to the Tb 3+ center results in a characteristic ratiometric response: quenching of the emission at 548 nm and concomitant enhancement at 650 nm. The probe exhibits excellent analytical performance with a linear range of 0.1―40 μM, a detection limit of 0.038 μM, and high selectivity over structurally similar interferents. Its practical applicability is demonstrated by reliable quantification of TMP in real samples, including sauce-flavor Baijiu, sake, and human urine, with recoveries of 94.54–104.82% and relative standard deviations below 10.57%. Significance This work establishes a pioneering optical sensing approach for TMP detection, providing a valuable analytical tool for flavor assessment in alcoholic beverages and pharmacological monitoring in biological systems.
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CELLULOSE
·
2023
·Vol. 30
·期 8
·5187-5200
·
IF 5.7
Li Jie,Lu Dechan,Yang Jinglei,You Ruiyun,Chen Jingbo,Weng Jingzheng,Lu Yudong
We developed a method for the fabrication of in-situ reduced bacterial cellulose membrane (BCM) for the simple synthesis of three-dimensional (3D) flexible SERS substrate. Ag NPs with high density were in-situ grown and uniformly distributed on the 3D BCM nanofibers through a controlled “silver mirror reaction” and “volume shrinkage” treatment, which created highly stable and uniform hot spots in the networked SERS substrate. “Hydrogel” BCM was formed by soaking in ammonia solution to hydrolyze the hemiacetal structure of cellulose, followed by in-situ reduction in silver trifluoroacetate (CF 3 COOAg) solution, the BCM enriched with silver nanospheres (Ag NPs-BCM) substrate was prepared. The results showed that the optimized Ag NPs-BCM substrate exhibited high SERS activity, and displayed an average EF of 5.3 × 10 8 and RSD of 11.0%, especially when protected from light for 45 days, the SERS performance kept steady. The Ag NPs-BCM substrate was used to detect glutathione (GSH). Interestingly, adding Ellman's reagent (DTNB) to serum, we clearly distinguished the amount of GSH content in cancer and normal using Ag NPs-BCM substrate. This has provided the opportunity to use flexible substrate in rapid biomedical assays.
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Arabian Journal of Chemistry
·
2023
·
IF 6
Bushra Rafique,Rizwan Ullah Khan,Aysha Sarfraz Rizvi,Muhammad Irfan,Ghulam Murtaza,Lili Qiu,Min Xue,Zihui Meng
There is a demand for simple, selective, and efficient assays for the determination of clinically important metabolites such as creatinine for healthcare. Creatinine is the by-product of muscle energy metabolism and is excreted by the kidneys. To measure creatinine in the human serum, a creatinine imprinted photonic crystal hydrogel (CIPC hydrogel) for naked-eye detection is developed. CIPC hydrogel utilizes polystyrene-based two-dimensional (2D) photonic crystal colloidal arrays (PCs-array) embedded in the polyacrylamide hydrogel containing methacrylic acid which imprinted the creatinine template. The nanocavities in the hydrogel produced after the removal of the template bind to and recognize creatinine in the serum samples. The binding is selective and specific for creatinine. The binding is observed as shrinkage of the hydrogel volume and a decrease in the particle spacing which is monitored through changes in the Debye diffraction ring diameter and a visible blue-green to blue color shift. The binding event and the mechanism are investigated by molecular dockings. The CIPC sensor demonstrates a limit of detection (LOD) of 2.45 ± 1.6 µM, a linear detection range (25–500 µM), and recovery from 85.6 % to 99.9 % in the serum samples. CIPC hydrogel is available for the rapid and quantitative onsite detection of creatinine in the human serum sample.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 19
·e53895
·
IF 3
Xingtong Gao,Kai Liu,Peng Liu,Xiaoyu Bai,Aixiang Li,Zijian Lyu,Qiuhong Li
In this paper, acrylic acid (AA) and sodium styrene sulfonate (SSS) were grafted onto the surface of cellulose acetate (CA) by free radical polymerization to obtain a heparin unit-containing graft copolymer CA-g-P(AA/SSS). It was used to coat the adsorbent resin for disposable hemoperfusion devices to improve blood compatibility. The adsorption capacity of the modified CA-coated adsorbent resin to creatinine, Vitamin B12, bilirubin, and imidacloprid was tested. The results showed that the modified CA-coated adsorbent resin had a good adsorption effect on small and medium-sized molecules. The hemolysis and coagulation tests showed that the CA-g-P(AA/SSS) coating could greatly improve the blood compatibility of the adsorbent resin. The hemolysis rate was 1.6%, which met the requirement of international standards for the hemolysis rate of blood contact equipment less than 5%. The coagulation index was higher than 1.55, which was comparable to the heparin-preflashed HA130 currently in use. In addition, the CA-g-P(AA/SSS)-coated adsorbent resin has good biocompatibility, no pyrogenic reaction, low vitro cytotoxicity, and no acute systemic toxicity. The resin has a good application prospect in uremia, hyperbilirubinemia, and rapid treatment of pesticide poisoning.
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Advanced Science
·
2023
·Vol. 10
·期 15
·2206494
·
IF 15.1
Shaowei Xie,Xiaochen Fei,Jiayi Wang,Yi-Cheng Zhu,Jiazhou Liu,Xinxing Du,Xuesong Liu,Liang Dong,Yinjie Zhu,Jiahua Pan,Baijun Dong,Jianjun Sha,Yu Luo,Wenshe Sun,Wei Xue
High-throughput metabolic fingerprinting has been deemed one of the most promising strategies for addressing the high false positive rate of prostate cancer (PCa) diagnosis in the prostate-specific antigen (PSA) gray zone. However, the current metabolic fingerprinting remains challenging in achieving high-precision metabolite detection in complex biological samples (e.g., serum and urine). Herein, a novel self-assembly MoS 2 /MXene heterostructure nanocomposite with a tailored doping ratio of 10% is presented as a matrix for laser desorption ionization mass spectrometry analysis in clinical biosamples. Notably, owing to the two-dimensional architecture and doping effect, MoS 2 /MXene demonstrates favorable laser desorption ionization performance with low adsorption energy, which is evidenced by efficient urinary metabolic fingerprinting with an enhanced area under curve (AUC) diagnosis capability of 0.959 relative to that of serum metabolic fingerprinting (AUC = 0.902) for the diagnosis of PCa in the PSA gray zone. Thus, this MoS 2 /MXene heterostructure is anticipated to offer a novel strategy to precisely and noninvasively diagnose PCa in the PSA gray zone.
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