JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
Yichong Wang,Danyan Li,Luqing Zhao,Jixiang Liu,Dan Dou,Nian Liu,Yudi Zhuo,Shengsheng Zhang
Ethnopharmacological relevance Yinxu Weitong Capsule (YXWTC) is a Chinese patent medicine used to treat chronic gastritis. However, its efficacy and mechanisms of action in treating precancerous lesions of gastric cancer (PLGC) remain unclear. Aim of the study To evaluate the effects of YXWTC on PLGC and explore the underlying mechanisms. Materials and methods YXWTC components were identified using ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole-exactive orbitrap mass spectrometry. A PLGC animal model was established and the protective effects of YXWTC on the gastric mucosa in PLGC rats were evaluated using hematoxylin and eosin (H&E), Alcian blue-periodic acid-Schiff and Alcian blue-high iron diamine staining, and transmission electron microscopy (TEM). The vital organs of the rats were examined using H&E staining to evaluate biosafety. Network pharmacology identified potential targets and pathways of YXWTC in PLGC treatment, followed by molecular docking validation. Various techniques, including enzyme-linked immunosorbent assay, real-time quantitative reverse transcription PCR, Western blotting, immunohistochemistry, apoptosis detection, and reactive oxygen species fluorescence staining were employed to elucidate the underlying mechanisms. Results In total, 340 YXWTC components were identified. YXWTC effectively improves gastric mucosal pathology in rats with PLGC. Network pharmacology identified 403 targets common to PLGC and YXWTC. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses identified 2,323 biological processes and 206 signaling pathways, respectively. Molecular docking revealed that the primary target proteins and major drug molecules exhibited strong binding affinities. Animal studies demonstrated that YXWTC inhibited the IL-6/STAT3 pathway, promoted mitochondrial apoptosis, and induced ROS release. Conclusions We verified the pharmacodynamic effects of YXWTC in PLGC. In summary, the effects are mediated by inhibition of the IL-6/STAT3 pathway, promotion of mitochondrial apoptosis, and induction of ROS release.
研究领域:
药理学
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肿瘤学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CELL BIOCHEMISTRY AND BIOPHYSICS
·
2024
·
IF 1.8
Jiang Qianqian,Fan Guoxia,Wu Kaiwei
Chronic atrophic gastritis (CAG) is a critical initial step in gastric cancer tumorigenesis accompanied by high malignancy. Erianin has been proposed as a promising agent in treating precancerous lesions of gastric cancer. Considering that little work has been implemented concerning the specific role and possible regulatory mechanism of Erianin in CAG, the goal of the study is to disclose the effects and mechanism of erianin on the malignant transformation in the process of CAG. CAG cell model was generated in human gastric epithelium GES-1 cells induced by Nmethyl-N’-nitro-N-nitrosoguanidine (MNNG). CCK-8 method determined cell viability. ELISA and corresponding assay kits severally appraised the contents of inflammatory cytokines and oxidative stress markers. Cellular reactive oxygen species (ROS) formation was measured by flow cytometry analysis using DCFH-DA probe. GFP-LC3 immunofluorescence staining and Western blotting evaluated autophagy. Also, Western blotting analyzed the expression of components in mitogen activated protein kinase (MAPK)/mechanistic target of rapamycin (mTOR) signaling. The results manifested that MNNG treatment diminished the viability and autophagy whereas intensified the inflammation and oxidative stress in GES-1 cells, which were all reversed by Erianin. Besides, Erianin blocked mTOR/MAPK signaling in MNNG-exposed GES-1 cells. Autophagy inhibitor 3-methyladenine (3-MA) or p38 MAPK agonist asiatic acid partially counteracted the protection elicited by Erianin against viability loss, inflammatory reaction as well as oxidative stress in MNNG-induced GES-1 cells. Combined with the findings, Erianin might mediate autophagy to improve MNNG-elicited CAG via MAPK/mTOR signaling.
研究领域:
肿瘤学
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胃肠病学
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药理学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
NanoImpact
·
2025
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IF 5.5
Yun Wang,Chen Li,Te Ba,Shengyuan Wang,Langzhi He,Zhangjian Chen,Ji Pu,Xiaoxing Cui,Guang Jia
Titanium dioxide nanoparticles (TiO 2 NPs) have promising applications in food additives and pharmaceutical dressings, raising concerns about their oral safety. The current studies mainly focus on healthy groups, and the effect of TiO 2 NPs on the patient population is rarely known. Here, a comprehensive toxicity study of TiO 2 NPs (75 ± 15 nm, anatase) in gastric ulcer rats ( male 8-week old Sprague-Dawley rats) is reported following oral exposure at dose of 0, 10, 50, 200 mg/kg body weight per day for 30 days. The gastric ulcer rats were produced by submucosal injection of acetic acid solution into the rat stomach. The healthy rats were used as the normal control. We evaluated nanoparticle biodistribution, systemic toxicity, and gastrointestinal function indices in the rats. Our findings indicate that oral administration of TiO 2 NPs resulted in minimal intestinal absorption and transport with limited systemic organ toxicity. The internalization of TiO2 NPs and activation of mast cells in the stomach tissues, along with the low serum levels of histamine and IgE, suggest a localized allergic reaction rather than a systemic one. Furthermore, the notably reduced plasma levels of D-lactate and the activity of diamine oxidase (DAO) indicated the upregulation of intestinal barrier function. These statistically significant results indicated that gastrointestinal dysfunction was the main performance of the oral toxicity of TiO 2 NPs on gastric ulcer rats, emphasizing the importance of controlling the intake of TiO 2 NPs in patients with gastric ulcers.
研究领域:
毒理学
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消化病学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Chenzhao Wang,Yuhao Jiang,Zhiyu Wu,Xiujuan Wang,Jiawei Yang,Junxian Li,Shanshan Wei,Guoying Sun,Zhibing Wang
Salivary pepsin has emerged as a promising biomarker for rapid screening and diagnosis of gastroesophageal reflux disease (GERD). Pepsin mainly exists in strongly acidic environments and exhibits its highest activity. However, the poor stability of most fluorescent sensors in strong acidic environments brings a significant challenge for pepsin detection. Herein, an innovative biosensor was developed for the highly specific and sensitive detection of pepsin on the basis of green-emitting ionic liquid-based carbon dots (G-IL-CDs) conjugated with whey proteins (WPs). The G-IL-CDs exhibited aggregation-induced fluorescence enhancement when interacting with WP, and the fluorescence intensity decreased after incubation with pepsin due to the disruption of the aggregation structure. This strategy is highly selective for pepsin due to the strongly acidic environment in which other proteases are inactivated. Under optimal experimental conditions, this biosensor successfully detected pepsin in real human saliva with a satisfying recovery. Furthermore, this study not only developed a CDs-based sensor for detecting pepsin but also laid a solid theoretical foundation for the future development of novel biosensors combining CDs and proteins.
研究领域:
生物技术
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Bingqian Yang,Kaikai Li,Meng Niu,Jianying Wei,Siming Zhao,Caihua Jia,Yan Xu
Wheat bran is an abundant yet underutilized agricultural byproduct. Herein, the insoluble dietary fiber from wheat bran (WBIDF) was ultra-milled to investigate its impact on physicochemical properties and gastrointestinal emptying. SEM and CLSM showed that the laminar structure of WBIDF was disrupted as the particle size was significantly reduced. In the similar characteristic peaks appearing at 3410, 2925, 1635, 1041, and 895 cm −1 in the FT-IR spectra and at 2940, 1593, 1080, and 526 cm −1 in the Raman spectra, the peak intensity was increased as the particle size decreased. It may be that the hydrogen bonding between cellulose, hemicellulose, or other macromolecules was enhanced. X-ray diffraction showed cellulose type I results for all five samples. Correspondingly, the water-holding, swelling, and oil-holding capacities increased by 75.33 %, 52.62 %, and 75.00 %, respectively, in WBIDF-CW 1.8 compared with WBIDF-CWy. Additionally, smaller particle sizes had lower viscosity, thereby enhancing intestinal propulsion and gastric emptying rates. Enhanced contact of the cecal tissue growth factor with the intestinal mucosa delayed ghrelin secretion and stimulated the secretion of motilin, gastrin, and cholecystokinin. In conclusion, the particle sizes of WBIDF were reduced through ultramicro-grinding, leading to altered structure, enhanced hydration and oil-holding capacities, decreased viscosity, and improved gastrointestinal emptying capacity.
研究领域:
食品科学
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营养学
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胃肠病学
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显微镜学
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光谱学
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动物生理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2024
·Vol. 25
·期 5
·2817
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IF 4.9
Gengchen Xie,Bo Lei,Zhijie Yin,Fei Xu,Xinghua Liu
Our previous study has reported that metastasis-associated protein 2 (MTA2) plays essential roles in tumorigenesis and aggressiveness of gastric cancer (GC). However, the underlying molecular mechanisms ofMTA2-mediated GC and its upstream regulation mechanism remain elusive. In this study, we identified a novel circular RNA (circRNA) generated from theMTA2gene (circMTA2) as a crucial regulator in GC progression. CircMTA2was highly expressed in GC tissues and cell lines, and circMTA2promoted the proliferation, invasion, and metastasis of GC cells both in vitro and in vivo. Mechanistically, circMTA2interacted with ubiquitin carboxyl-terminal hydrolase L3 (UCHL3) to restrainMTA2ubiquitination and stabilizeMTA2protein expression, thereby facilitating tumor progression. Moreover, circMTA2was mainly encapsulated and transported by exosomes to promote GC cell progression. Taken together, these findings uncover that circMTA2suppressesMTA2degradation by interacting with UCHL3, thereby promoting GC progression. In conclusion, we identified a cancer-promoting axis (circMTA2/UCHL3/MTA2) in GC progression, which paves the way for us to design and synthesize targeted inhibitors as well as combination therapies.Keywords:CircRNAs;MTA2;gastric cancer;UCHL3;ubiquitination
研究领域:
分子生物学
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肿瘤学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
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2015
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IF 0.906
Caihui Wang,Wen Su,Xingli Su,Guojun Ni,Tao Liu,Yi Kong
The gastric mucosa protection effect of three natural plant extracts, Hericium erinaceus (HE), Centella asiatica (CA) and Amomum villosum (AV), were evaluated using the indomethacin damage model. Compared with a single extract, a combination of HE/CA/AV, especially with the ratios of 80:10:10, 45:45:10 and 45:10:45, showed significant synergistic effects for protection of the gastric mucosa with gastric ulcer inhibition rates of 97.8 ± 0.7%, 86.5 ± 2.8% and 86.1 ± 3.6%, respectively. Microscopic appearances of the gastric mucosa were carried out to help confirm the results.
研究领域:
药理学
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胃肠病学
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天然产物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Haibo Shi,Ruyi Zhang,Jiabao Zheng,Xianqi Yao,Wei Wang,Igor Tomasevic,Weizheng Sun
The effects of NaCl partially substituted by KCl, MgCl 2 , and KCl/MgCl 2 on the gel characteristics, oral mastication, and in vitro gastric digestion of myofibrillar protein (MP) gel were investigated. The results indicated that partial substitution of NaCl by KCl or MgCl 2 did not cause obvious deterioration of gel properties within 30 % substitution levels. MgCl 2 substitution significantly increased the surface hydrophobicity of MP, and formed homogeneous network structure for effective limitation of moisture loss. The MgCl 2 -substituted gel exhibited relatively lower viscosity and hardness for better swallowing. The high pepsin diffusivity in MgCl 2 -substituted gel (59.57 ± 4.28 μm 2 /s, 30 %-Mg group) improved protein hydrolysis, and produced more shorter peptides due to the loose and relatively uniform structure compared to those of the gels only containing NaCl and partial KCl. Therefore, the changes of textural parameters, microstructure, and water mobility in sodium-reduced gel with different substitution levels could influence the subsequent gastric digestion characteristics.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Chunhuan Liu,Yumeng Shi,Yuchun Liu,Yan Liu,Chenjing Yang,Huan Chen,Cheng Yang,Rong Liang
Hydrogel beads have been explored to be normal platform for nutrition encapsulation and delivery. However, hydrogel beads are naturally denser than water, which make hydrogel beads limite in some floating applications such as gastric floating systems. Here, we designed alginate-based floating beads featuring a core-shell structure, which were composed of a vegetable oil core encapsulated within an alginate network shell, utilizing coaxial co-extrusion microfluidic devices. The shell thickness and core size of the core-shell beads (with the size of 2.80–3.40 mm) can be controlled by simply adjusting the inner phase flow rate ( Q in ) and outer phase flow rate ( Q out ). Notably, the fabrication of floating core-shell beads was achievable with Q in ≥ 40 μ L/min and Q out set at 300 μ L/min. The Hen's egg test – chorioallantoic membrane (HET-CAM) test showed the floating core-shell beads were non-irritant, indicating their safety and biocompatibility properties. Given their floating properties and biocompatibility, these floating core-shell beads hold great potential for applications in the fields of gastric-retentive nutrition delivery. Following that, the floating core-shell beads were further encapsuled with pepsin. They displayed possessed satisfactory pepsin activity (90%) even after 15 days, suggesting that floating core-shell beads can protect the enzyme ingredients against damage. Interestingly, the floating core-shell beads exhibited both efficient sustained-release of pepsin and floating ability in simulated gastric solution. This work sheds light on the design and development of controllable floating core-shell hydrogel beads for various floating system applications.
研究领域:
材料科学
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药学
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生物技术
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 16
·2800
·
IF 5.1
Yangzu Gao,Haoyu Li,Qian Wu,Bang Chen,Kangzhen Xu,Cong Li,Yehua Shen
The functional exploration of natural foods, coupled with the increasing prevalence of gastrointestinal motility disorders and the associated therapeutic challenges, has generated significant interest in this field. This study aims to investigate the ameliorative effects of the extract fromPugionium cornutum(L.) Gaertn (EAEPC), a traditional edible vegetable in northwest China’s desert region, on atropine-induced gastroparesis in mice, as well as to elucidate its mechanism in terms of the gut microbiota and major metabolites. The findings indicate that EAEPC effectively reduces the rate of pigment residual in the stomach while shortening the gastrointestinal transit time and alleviating other symptoms associated with atropine-induced gastroparesis. These effects may be mediated through modulation of the expression levels of major intestinal metabolites, such as short-chain fatty acids (SCFAs), bile acids (BAs), and L-tryptophan, alongside remodeling of both the diversity and relative abundance of the gut microbiota. Furthermore, correlation analyses were conducted on significantly altered strains and metabolites to clarify their interactions. Moreover, the chemical constituents of EAEPC were identified by UPLC-Q-TOF-MS/MS, and the key active components responsible for improving gastroparesis were predicted through network pharmacology approaches and validated experimentally. These results provide a foundation for further research into the functions ofPugioniumand offer scientific support for developing natural plant-based strategies aimed at treating gastrointestinal motility disorders.
研究领域:
消化病学
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天然产物药理学
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肠道微生态学
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代谢组学
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分子药理学
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植物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Translational Oncology
·
2025
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IF 4.1
Zhe Qin,Fangzhou Ye,Jiayi Wang,Jun Jiang,Xiaohong Zhang,Huanqing Li,Li Feng
Background Cisplatin resistance significantly impedes the treatment of gastric cancer (GC). This work examined the possible therapeutic target status and function of BUB1B in controlling cisplatin resistance. Methods Following the identification of differentially expressed genes (DEGs), protein-protein interaction (PPI) network analysis was conducted using datasets from the Cancer Genome Atlas-stomach adenocarcinoma (TCGA-STAD), GSE51575, and GSE79973. Functional tests assessed the effect of BUB1B overexpression and knockdown on the GC cells. Enrichment analysis and RNA-seq identified pathways linked to BUB1B . Additionally, the function of BUB1B in GC cells resistant to cisplatin in regulating DNA repair was examined, as its relationship with Rad51 inhibitor (B02) in regulating cell cycle, proliferation, and apoptosis. The combined effects of Rad51 suppression and BUB1B overexpression on tumor development in cisplatin-resistant GC cells were further validated in vivo xenograft models. Results Significant overexpression of six critical overlapping genes was seen in GC tissues. The GC cell invasion, migration, and proliferation processes were improved by BUB1B overexpression, whereas BUB1B knockdown prevented these outcomes. Genes involved in DNA repair were downregulated by BUB1B knockdown, according to an RNA-seq study. BUB1B overexpression boosted cell survival via modulating cell cycle proteins, but BUB1B knockdown hampered DNA repair and increased death in cisplatin-resistant GC cells. Overexpression of BUB1B enhanced tumor development in vivo and counteracted the inhibitory effects of B02 on cell growth. Conclusion BUB1B enhances cisplatin resistance in gastric cancer by regulating DNA repair and cell cycle progression, suggesting that targeting BUB1B may be a feasible therapeutic strategy.
研究领域:
肿瘤学
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分子生物学
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癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Research
·
2025
·
IF 9.6
Yunsheng Lu,Yibin Huang,Chenchen Mao,Pengfei Shan,Chenkang Wu,Jiongzhou Zhu,Yujie Lin,Zhongyu Li,Mingdong Lu
Silencing NOP2/Sun RNA methyltransferase family member 2 (NSUN2) effectively inhibits gastric cancer (GC) progression but is limited by RNase degradation, rapid renal clearance, and low uptake. Based on the characteristic high levels of reactive oxygen species (ROS) in the tumor microenvironment, this study designed and synthesized a novel ROS-responsive ferrocene nanoparticle loaded with siNSUN2 (PRPFc@siNSUN2). Under ROS conditions, the nanoparticle disintegrates to release siNSUN2. Characterization by proton nuclear magnetic resonance, transmission electron microscopy, dynamic light scattering, and ultraviolet–visible spectrophotometry revealed that PRPFc@siNSUN2 is spherical, with an average diameter of 88.79 ± 1.14 nm, an encapsulation efficiency of 83.10%, and a drug loading capacity of 13.85%. Moreover, these nanoparticles demonstrated excellent stability and, under hydrogen peroxide conditions, exhibited structural disruption leading to the release of siNSUN2, thereby confirming their high ROS responsiveness. In vitro, PRPFc@siNSUN2 markedly enhanced the inhibition of GC cell proliferation, migration, and invasion, and promoted apoptosis, accompanied by increased intracellular ROS and improved siNSUN2 uptake. In vivo studies further confirmed that PRPFc@siNSUN2 markedly enhanced the therapeutic efficacy of siNSUN2 against GC, while exhibiting low cytotoxicity and good biocompatibility. Overall, our findings indicate that PRPFc@siNSUN2, with its favorable morphology, stability, and ROS-triggered release, substantially improves the anti-GC effects of siNSUN2 by inhibiting GC cell proliferation, migration, and invasion, as well as by promoting apoptosis. These results support NSUN2 as a promising therapeutic target and underscore the potential of PRPFc@siNSUN2 nanoparticles in drug delivery, offering a novel strategy to improve clinical outcomes for GC patients.
研究领域:
肿瘤学
·
分子生物学
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药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）